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The map label for this gene is purF [H]

Identifier: 220933044

GI number: 220933044

Start: 2408710

End: 2410152

Strand: Reverse

Name: purF [H]

Synonym: Hore_22120

Alternate gene names: 220933044

Gene position: 2410152-2408710 (Counterclockwise)

Preceding gene: 220933045

Following gene: 220933043

Centisome position: 93.48

GC content: 44.84

Gene sequence:

>1443_bases
ATGCCATATGAAATAATGAGCGGAAAACCCAGGGAAGAGTGTGGTGTATTTGGTATTTTTTCACCGGACAGGAATGATGC
CGGTAGTCTTATCTACCTCGGGTTGCATTCTTTACAGCACCGGGGGCAGGAGAGTGCCGGTATTGCTGTAAGCCAGGATA
AAGGGATTAACCTCTATAAGCGGATGGGTCTGGTAGATAATGTTTTTAATAAATCCATCATTGAGACCCTGTCGGGTTGG
GCAGGGATTGGTCATGTCAGGTATTCAACTACCGGTTCCAGCCTGGCTGCTAATTCCCAGCCCATCCTGATTAATTCAAT
CAAGGGTCAGGTAGCCCTGGCCCACAATGGTAACCTTGTTAATGGTTATGAATTAAGGATTGCCCTCGAGAAAAAGGGTT
CTGTTTTTCATTCTACCCTGGATACGGAAGTGATTGCCCACCTTATTGCCCGTTCTCAATACAACGATATCCCCAGTGCT
CTTCTCGATAGTTTAAAAACAATTGAAGGGGCCTATTCTCTTGTAGTTTTAACAAGGGATAAGCTTATTGGCGTCCGGGA
TCCCCGGGGTTTCAGGCCTCTGGTCATGGGAAAGCTGGGTGAGGGCATAGTCCTGGCTTCAGAAACCTGTGCCCTGAATA
TAATCGGGGCTGAATATGTACGGGATATTGAACCCGGGGAGATGGTAGTGATAGATGAAAATGGTGTTCAGAGCTATTCC
TTTAATCCGGAGGTTGAACCCCGGTTCTGTATATTTGAGTATATCTATTTTGCCCGTCCCGACAGCTCTTTTAATGGAAA
TAACGTCCATCTGATCCGTAAAGATATGGGGAGGCAGCTGGCCCGGGAAGCCGGTGACCTCCTGAAAAAAATAGATGTAG
TCGTTCCTGTACCTGATTCGGGAATTTCTTCAGCCCAGGGTTTTGCTGAAGAAAGCGGCCTTCCGTTTCAATATGGCCTT
ATTAAAAACAGATATGTGGGTAGAACCTTTATTAATCCTACCCAGGAAATGAGGAATCTGAAGGTTAGAATGAAATTAAA
TCCTATTAAGAATATTATAAGGGGGAAGAATATTGCTCTGATAGATGATTCAATTGTCAGGGGAACAACCAGCCGTCAGA
TCGTTAATATGCTTAAAGAGGCCGGGGCCCGGGACGTTCATCTCTTTGTCAGTTCACCTCCGGTAACCCATTCCTGTTAT
TATGGTCTTGATACCTCCAATAGGCAGGAATTAATTGCTTCCCGGCTAAATGTCAAAGATATTGCCCGGGAAATTGGAGC
TGATTCGTTAACCTATCTCAGTATCGAGGGATTATTATCTACTGTTGAGAGAAAAGAAAAAGGTTGCTGTGTCGCCTGTT
TTTCGGGTGATTACCCTACCAGGACCGGAAATGGGAAACTGGCTTTAGAAAAAAACGTCCCTGAGATTAAATCAGGACAA
TAA

Upstream 100 bases:

>100_bases
ATAGAAATATCGGTAAAACGGGTGGGAACCGACTAATAATTGATAGTTATATTGACCTGGAGCTCACTGAAATAAAAGAT
AGGTGGGAGAGGACAATACC

Downstream 100 bases:

>100_bases
TCTGATACCGGGCAAGAAATATTTAAAAATGGAGGGGTTTAATGAAAAAGGGCATAACCTATGCTGATTCGGGGGTAAAT
ATTGATAAAGGTAACGAGTT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MPYEIMSGKPREECGVFGIFSPDRNDAGSLIYLGLHSLQHRGQESAGIAVSQDKGINLYKRMGLVDNVFNKSIIETLSGW
AGIGHVRYSTTGSSLAANSQPILINSIKGQVALAHNGNLVNGYELRIALEKKGSVFHSTLDTEVIAHLIARSQYNDIPSA
LLDSLKTIEGAYSLVVLTRDKLIGVRDPRGFRPLVMGKLGEGIVLASETCALNIIGAEYVRDIEPGEMVVIDENGVQSYS
FNPEVEPRFCIFEYIYFARPDSSFNGNNVHLIRKDMGRQLAREAGDLLKKIDVVVPVPDSGISSAQGFAEESGLPFQYGL
IKNRYVGRTFINPTQEMRNLKVRMKLNPIKNIIRGKNIALIDDSIVRGTTSRQIVNMLKEAGARDVHLFVSSPPVTHSCY
YGLDTSNRQELIASRLNVKDIAREIGADSLTYLSIEGLLSTVERKEKGCCVACFSGDYPTRTGNGKLALEKNVPEIKSGQ

Sequences:

>Translated_480_residues
MPYEIMSGKPREECGVFGIFSPDRNDAGSLIYLGLHSLQHRGQESAGIAVSQDKGINLYKRMGLVDNVFNKSIIETLSGW
AGIGHVRYSTTGSSLAANSQPILINSIKGQVALAHNGNLVNGYELRIALEKKGSVFHSTLDTEVIAHLIARSQYNDIPSA
LLDSLKTIEGAYSLVVLTRDKLIGVRDPRGFRPLVMGKLGEGIVLASETCALNIIGAEYVRDIEPGEMVVIDENGVQSYS
FNPEVEPRFCIFEYIYFARPDSSFNGNNVHLIRKDMGRQLAREAGDLLKKIDVVVPVPDSGISSAQGFAEESGLPFQYGL
IKNRYVGRTFINPTQEMRNLKVRMKLNPIKNIIRGKNIALIDDSIVRGTTSRQIVNMLKEAGARDVHLFVSSPPVTHSCY
YGLDTSNRQELIASRLNVKDIAREIGADSLTYLSIEGLLSTVERKEKGCCVACFSGDYPTRTGNGKLALEKNVPEIKSGQ
>Mature_479_residues
PYEIMSGKPREECGVFGIFSPDRNDAGSLIYLGLHSLQHRGQESAGIAVSQDKGINLYKRMGLVDNVFNKSIIETLSGWA
GIGHVRYSTTGSSLAANSQPILINSIKGQVALAHNGNLVNGYELRIALEKKGSVFHSTLDTEVIAHLIARSQYNDIPSAL
LDSLKTIEGAYSLVVLTRDKLIGVRDPRGFRPLVMGKLGEGIVLASETCALNIIGAEYVRDIEPGEMVVIDENGVQSYSF
NPEVEPRFCIFEYIYFARPDSSFNGNNVHLIRKDMGRQLAREAGDLLKKIDVVVPVPDSGISSAQGFAEESGLPFQYGLI
KNRYVGRTFINPTQEMRNLKVRMKLNPIKNIIRGKNIALIDDSIVRGTTSRQIVNMLKEAGARDVHLFVSSPPVTHSCYY
GLDTSNRQELIASRLNVKDIAREIGADSLTYLSIEGLLSTVERKEKGCCVACFSGDYPTRTGNGKLALEKNVPEIKSGQ

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=507, Percent_Identity=39.2504930966469, Blast_Score=345, Evalue=5e-95,
Organism=Homo sapiens, GI4826742, Length=186, Percent_Identity=31.7204301075269, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788651, Length=482, Percent_Identity=40.0414937759336, Blast_Score=301, Evalue=4e-83,
Organism=Escherichia coli, GI1790167, Length=230, Percent_Identity=29.5652173913043, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17554892, Length=484, Percent_Identity=37.396694214876, Blast_Score=308, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI17532897, Length=185, Percent_Identity=32.4324324324324, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17532899, Length=185, Percent_Identity=32.4324324324324, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17539970, Length=184, Percent_Identity=31.5217391304348, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323958, Length=483, Percent_Identity=36.8530020703934, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6322745, Length=148, Percent_Identity=31.7567567567568, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24659598, Length=485, Percent_Identity=42.0618556701031, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI28573187, Length=489, Percent_Identity=41.7177914110429, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24659604, Length=464, Percent_Identity=42.4568965517241, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI21357745, Length=183, Percent_Identity=29.5081967213115, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 52684; Mature: 52553

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYEIMSGKPREECGVFGIFSPDRNDAGSLIYLGLHSLQHRGQESAGIAVSQDKGINLYK
CCCCCCCCCCHHHCCEEEEECCCCCCCCCEEEEEHHHHHHCCCCCCCEEEECCCCCHHHH
RMGLVDNVFNKSIIETLSGWAGIGHVRYSTTGSSLAANSQPILINSIKGQVALAHNGNLV
HHCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEECCCEEEEEECCCEE
NGYELRIALEKKGSVFHSTLDTEVIAHLIARSQYNDIPSALLDSLKTIEGAYSLVVLTRD
CCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEECC
KLIGVRDPRGFRPLVMGKLGEGIVLASETCALNIIGAEYVRDIEPGEMVVIDENGVQSYS
CEEECCCCCCCCCHHHHCCCCCEEEEECCEEEEEECHHHHHCCCCCCEEEEECCCCEEEC
FNPEVEPRFCIFEYIYFARPDSSFNGNNVHLIRKDMGRQLAREAGDLLKKIDVVVPVPDS
CCCCCCCCCEEEEEHHEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCEEEEECCCC
GISSAQGFAEESGLPFQYGLIKNRYVGRTFINPTQEMRNLKVRMKLNPIKNIIRGKNIAL
CCCHHCCCHHHCCCCEEECCCCCCCCCCCCCCHHHHHCCEEEEEEECHHHHHHCCCCEEE
IDDSIVRGTTSRQIVNMLKEAGARDVHLFVSSPPVTHSCYYGLDTSNRQELIASRLNVKD
ECCHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHCCHHH
IAREIGADSLTYLSIEGLLSTVERKEKGCCVACFSGDYPTRTGNGKLALEKNVPEIKSGQ
HHHHHCCCCEEEEEHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCC
>Mature Secondary Structure 
PYEIMSGKPREECGVFGIFSPDRNDAGSLIYLGLHSLQHRGQESAGIAVSQDKGINLYK
CCCCCCCCCHHHCCEEEEECCCCCCCCCEEEEEHHHHHHCCCCCCCEEEECCCCCHHHH
RMGLVDNVFNKSIIETLSGWAGIGHVRYSTTGSSLAANSQPILINSIKGQVALAHNGNLV
HHCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEECCCEEEEEECCCEE
NGYELRIALEKKGSVFHSTLDTEVIAHLIARSQYNDIPSALLDSLKTIEGAYSLVVLTRD
CCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEECC
KLIGVRDPRGFRPLVMGKLGEGIVLASETCALNIIGAEYVRDIEPGEMVVIDENGVQSYS
CEEECCCCCCCCCHHHHCCCCCEEEEECCEEEEEECHHHHHCCCCCCEEEEECCCCEEEC
FNPEVEPRFCIFEYIYFARPDSSFNGNNVHLIRKDMGRQLAREAGDLLKKIDVVVPVPDS
CCCCCCCCCEEEEEHHEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCEEEEECCCC
GISSAQGFAEESGLPFQYGLIKNRYVGRTFINPTQEMRNLKVRMKLNPIKNIIRGKNIAL
CCCHHCCCHHHCCCCEEECCCCCCCCCCCCCCHHHHHCCEEEEEEECHHHHHHCCCCEEE
IDDSIVRGTTSRQIVNMLKEAGARDVHLFVSSPPVTHSCYYGLDTSNRQELIASRLNVKD
ECCHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHCCHHH
IAREIGADSLTYLSIEGLLSTVERKEKGCCVACFSGDYPTRTGNGKLALEKNVPEIKSGQ
HHHHHCCCCEEEEEHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]