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The map label for this gene is purN [H]

Identifier: 220933042

GI number: 220933042

Start: 2407009

End: 2407626

Strand: Reverse

Name: purN [H]

Synonym: Hore_22100

Alternate gene names: 220933042

Gene position: 2407626-2407009 (Counterclockwise)

Preceding gene: 220933043

Following gene: 220933041

Centisome position: 93.39

GC content: 42.56

Gene sequence:

>618_bases
ATGGGCAATAAAATTAATCTGGCTGTATTTATTTCCGGGAATGGAACCAATCTTCAGGCAATTATAGATTCTATTAAAGC
TGGCAGGGTTGAGGCTGAATTAAAAATGGTGATCAGTGACAAAAAGAACGCCTATGGCCTGGTCAGGGCCGAAAAGGCCG
GCATTGAAAATATCTTTATTGATCCAGCTGATTTTAACTCCCGCCAGGGGTATGAGAAGGAATTGCTGGATTATCTGGAT
AAAAAGAATATAGACCTGGTAGCCCTGGCCGGCTTTATGCGTTTGTTAAGCCCCTATTTTATTAATCAGTTTTCAGGAAA
GATTATGAATATACATCCTTCATTGCTGCCCAGTTTTCCGGGGCTTCATGCCCAGAGGCAGGCCCTGGAATACGGGGTTA
AGGTCAGTGGTTGTACAGTTCATTTTGTAGATGAAGGAATGGATACTGGCCCCATCATCTTACAGGCTCCAGTCCCGGTT
TACAGTGACGATACAGAAGAAAGACTGGCTTCCCGGATAAGGGAGAAAGAACATGAACTTTACCCGGAAGCTATTCAGCT
TTTTGCTGAAAACAGGTTAACAATACAGGGTAGAAAGGTATATATAAATCAACCATAA

Upstream 100 bases:

>100_bases
GAAAATGTTATGTCAGATATAAAGGCCCGGGGAGAAAAAGTATACCATATTGGAGAAATAAATAGTATCGGTAAAAAAGA
GGGTAAGGTGGTTATCTACA

Downstream 100 bases:

>100_bases
TGTTCCTGTACGGTTTTTTTAAATACGGAGTGCCTGTGGCACAACCATAATGTTCCTGTACGGTTTTTTTAAATACGGAG
TGCCTGTGGCACAACCATAA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MGNKINLAVFISGNGTNLQAIIDSIKAGRVEAELKMVISDKKNAYGLVRAEKAGIENIFIDPADFNSRQGYEKELLDYLD
KKNIDLVALAGFMRLLSPYFINQFSGKIMNIHPSLLPSFPGLHAQRQALEYGVKVSGCTVHFVDEGMDTGPIILQAPVPV
YSDDTEERLASRIREKEHELYPEAIQLFAENRLTIQGRKVYINQP

Sequences:

>Translated_205_residues
MGNKINLAVFISGNGTNLQAIIDSIKAGRVEAELKMVISDKKNAYGLVRAEKAGIENIFIDPADFNSRQGYEKELLDYLD
KKNIDLVALAGFMRLLSPYFINQFSGKIMNIHPSLLPSFPGLHAQRQALEYGVKVSGCTVHFVDEGMDTGPIILQAPVPV
YSDDTEERLASRIREKEHELYPEAIQLFAENRLTIQGRKVYINQP
>Mature_204_residues
GNKINLAVFISGNGTNLQAIIDSIKAGRVEAELKMVISDKKNAYGLVRAEKAGIENIFIDPADFNSRQGYEKELLDYLDK
KNIDLVALAGFMRLLSPYFINQFSGKIMNIHPSLLPSFPGLHAQRQALEYGVKVSGCTVHFVDEGMDTGPIILQAPVPVY
SDDTEERLASRIREKEHELYPEAIQLFAENRLTIQGRKVYINQP

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=204, Percent_Identity=44.6078431372549, Blast_Score=179, Evalue=1e-45,
Organism=Homo sapiens, GI209869995, Length=204, Percent_Identity=44.6078431372549, Blast_Score=179, Evalue=1e-45,
Organism=Homo sapiens, GI209869993, Length=204, Percent_Identity=44.6078431372549, Blast_Score=179, Evalue=1e-45,
Organism=Escherichia coli, GI1788846, Length=199, Percent_Identity=45.7286432160804, Blast_Score=206, Evalue=6e-55,
Organism=Escherichia coli, GI1787483, Length=136, Percent_Identity=35.2941176470588, Blast_Score=93, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=182, Percent_Identity=43.4065934065934, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6320616, Length=195, Percent_Identity=31.2820512820513, Blast_Score=85, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24582400, Length=184, Percent_Identity=51.6304347826087, Blast_Score=199, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22940; Mature: 22808

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNKINLAVFISGNGTNLQAIIDSIKAGRVEAELKMVISDKKNAYGLVRAEKAGIENIFI
CCCEEEEEEEEECCCCCHHHHHHHHHCCCHHHEEEEEEECCCCCEEEEEECCCCCCEEEE
DPADFNSRQGYEKELLDYLDKKNIDLVALAGFMRLLSPYFINQFSGKIMNIHPSLLPSFP
CCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECHHHCCCCC
GLHAQRQALEYGVKVSGCTVHFVDEGMDTGPIILQAPVPVYSDDTEERLASRIREKEHEL
CCHHHHHHHHCCCEECCEEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
YPEAIQLFAENRLTIQGRKVYINQP
CHHHHHHHHCCCEEEECCEEEEECC
>Mature Secondary Structure 
GNKINLAVFISGNGTNLQAIIDSIKAGRVEAELKMVISDKKNAYGLVRAEKAGIENIFI
CCEEEEEEEEECCCCCHHHHHHHHHCCCHHHEEEEEEECCCCCEEEEEECCCCCCEEEE
DPADFNSRQGYEKELLDYLDKKNIDLVALAGFMRLLSPYFINQFSGKIMNIHPSLLPSFP
CCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECHHHCCCCC
GLHAQRQALEYGVKVSGCTVHFVDEGMDTGPIILQAPVPVYSDDTEERLASRIREKEHEL
CCHHHHHHHHCCCEECCEEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
YPEAIQLFAENRLTIQGRKVYINQP
CHHHHHHHHCCCEEEECCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]