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The map label for this gene is metG [H]

Identifier: 220932862

GI number: 220932862

Start: 2189439

End: 2191400

Strand: Reverse

Name: metG [H]

Synonym: Hore_20300

Alternate gene names: 220932862

Gene position: 2191400-2189439 (Counterclockwise)

Preceding gene: 220932863

Following gene: 220932861

Centisome position: 85.0

GC content: 39.55

Gene sequence:

>1962_bases
ATGTCCGAAAAAACATTTTATGTGACAACTCCTATTTACTATCCCAGTGACAGATTACACATTGGTCATGCCTATACCAC
TGTAGCAGCTGATACTCTGGCCAAATTCAAAGAGATGCAGGATTATGATACCATGTTTTTGACAGGTTCTGATGAACATG
GTCAGAAGATTGAGAGAAAAGCAAGTGAAGCGGGTAAAAAACCCGGAGAATATGTTGATGAAATTGTTGCTACTTTTAAG
GATTTATGGAAGAGACTTGGGGTGGATTATGATTACTTTATCAGGACAACCAACCCTGACCATGAAAAGGTGGTCCAGGA
GATTTTCAAAAAGATATATGAGAAGGGAGATATTTATAAAGGGAAATATGAAGGATGGTACTGTACTCCCTGTGAAACTT
TCTGGACTGAAAGGCAGTTAGAAGGGGAAAAGGTCTGTCCTGACTGTGGACGTCCGGTTGAATGGGTAGAAGAAGAGAGC
TATTTCTTTAAATTAAGTAAATATGCTGACCGTCTTCTGGAACATATTAAAAAACACCCGGAATTTATTCAACCTGAAAG
CCGTCGTAAAGAAATGATAAATTTCATTGAATCTGGCCTTGAAGACCTCAGTGTTTCCCGAACAACCTTTGACTGGGGAA
TACCGGTTCCCATAGATGATGATCATGTTATCTATGTGTGGTTTGATGCCTTAAGTAATTATATAAGCGCTATTGGGTAT
TTATCTGATGATAAAAAGTTTAATCATTACTGGCCGGCTGATGTCCATATTGTTGGTAAGGATATTCTGAGATTCCATAC
CATTATCTGGCCTATAATTTTAATGGCCCTCGACCTGCCCTTACCTAAAAAGGTGTTCGGCCATGGCTGGCTTTTAACTG
AAACCGGGAAGATGTCAAAATCTAAGGGGAATGTTGTTGATCCCAATAAACTTATAGATGATTTCGGGGTAGACCCGATA
AGGTATTATCTGTTACGTGAAGTTGCTTTTGGCCAGGACGGGACATACTCAACTGAGGCTTTAATTAAGAGAATTAATTC
TGATCTGGCCAATGACCTCGGGAATCTTCTGCACCGGACTGTTACTATGGTTGATAAGTACTTTGACGGGGTAATACCTG
AACCAGGTGTTGAGAAAGAGGTGGATAAAGACCTTAAGAATTATGCCCTGAAAACCCTGGATGAAATTGATAACAGTATG
GACAACCTCAAATATGCAGTAGCTCTGGAGTCATTATGGAATTTTATCAGGAGAAGTAATAAGTATATAGACCAGACAAC
CCCGTGGATTCTGGCCCGGGATGAGGATAAAAAAGACCGGTTGGGGACTGTTCTGTATAATTTAATTGAGGCCCTGAGAA
TTATTGCCGGTATGCTGAAACCATTTCTGGTGGAAACACCATACATGATTGGTGAACAGATTAATGCCAGGGATTTAATA
GAGAAAGCATCATGGAGTGATTTAAAACAATGGGGTCTACTATCTCCGGGAAGAAAAGTAAAAAAGGGTAAACCGATTTA
TCCCAGGATTGATATAGAAGAATATTTTGAAAGACTGAACAACAATGAAAATAAGGAAACCAGTGATAATAAAGGAAAAG
GAGGTAATGATATGGCAGAACAGGATAAAAATACAGGAACTATTTCGTTTAATGATTTTACTAAACTTGATTTAAGGGTA
GCTGAGGTCAAGGAAGCAGAAAAAATAGAGGGGAGTAATAAGCTCTTAAAGCTAAAAGTGTCTCTGGGAGACGAGGAAAG
ACAGGTGGTTGCCGGTATAGCCAAACACTATAATCCTGAGGAACTTGTTGGTAAAAAAGTAATCCTGGTGGCTAATCTTG
AACCGGCTACTATTTTTGGGGTAAAATCAGAAGGTATGGTGCTGGCTGCCTCCAATGACAGTGGTGAATTAACTCTGACT
ACAGTTGAGAAGGATATATCCAGTGGTAGTCAGGTAAAATAG

Upstream 100 bases:

>100_bases
TAGAAAAATGTATAGTGAGAAAGAGGTATAGAAAGCATCCATTAACATGTTTGCATAATGTTGTAGACAACTGAATTGTG
AAGGAAAGGAGATGAATATC

Downstream 100 bases:

>100_bases
TTAAACTTTTTCAGGAAGTAGAGTTTAAAACCCACCAGCCTTTTCTGTGGATGGTGGGTTTTTAATATCGATGATTATAC
TGGTACCATGATGATTTTTC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 653; Mature: 652

Protein sequence:

>653_residues
MSEKTFYVTTPIYYPSDRLHIGHAYTTVAADTLAKFKEMQDYDTMFLTGSDEHGQKIERKASEAGKKPGEYVDEIVATFK
DLWKRLGVDYDYFIRTTNPDHEKVVQEIFKKIYEKGDIYKGKYEGWYCTPCETFWTERQLEGEKVCPDCGRPVEWVEEES
YFFKLSKYADRLLEHIKKHPEFIQPESRRKEMINFIESGLEDLSVSRTTFDWGIPVPIDDDHVIYVWFDALSNYISAIGY
LSDDKKFNHYWPADVHIVGKDILRFHTIIWPIILMALDLPLPKKVFGHGWLLTETGKMSKSKGNVVDPNKLIDDFGVDPI
RYYLLREVAFGQDGTYSTEALIKRINSDLANDLGNLLHRTVTMVDKYFDGVIPEPGVEKEVDKDLKNYALKTLDEIDNSM
DNLKYAVALESLWNFIRRSNKYIDQTTPWILARDEDKKDRLGTVLYNLIEALRIIAGMLKPFLVETPYMIGEQINARDLI
EKASWSDLKQWGLLSPGRKVKKGKPIYPRIDIEEYFERLNNNENKETSDNKGKGGNDMAEQDKNTGTISFNDFTKLDLRV
AEVKEAEKIEGSNKLLKLKVSLGDEERQVVAGIAKHYNPEELVGKKVILVANLEPATIFGVKSEGMVLAASNDSGELTLT
TVEKDISSGSQVK

Sequences:

>Translated_653_residues
MSEKTFYVTTPIYYPSDRLHIGHAYTTVAADTLAKFKEMQDYDTMFLTGSDEHGQKIERKASEAGKKPGEYVDEIVATFK
DLWKRLGVDYDYFIRTTNPDHEKVVQEIFKKIYEKGDIYKGKYEGWYCTPCETFWTERQLEGEKVCPDCGRPVEWVEEES
YFFKLSKYADRLLEHIKKHPEFIQPESRRKEMINFIESGLEDLSVSRTTFDWGIPVPIDDDHVIYVWFDALSNYISAIGY
LSDDKKFNHYWPADVHIVGKDILRFHTIIWPIILMALDLPLPKKVFGHGWLLTETGKMSKSKGNVVDPNKLIDDFGVDPI
RYYLLREVAFGQDGTYSTEALIKRINSDLANDLGNLLHRTVTMVDKYFDGVIPEPGVEKEVDKDLKNYALKTLDEIDNSM
DNLKYAVALESLWNFIRRSNKYIDQTTPWILARDEDKKDRLGTVLYNLIEALRIIAGMLKPFLVETPYMIGEQINARDLI
EKASWSDLKQWGLLSPGRKVKKGKPIYPRIDIEEYFERLNNNENKETSDNKGKGGNDMAEQDKNTGTISFNDFTKLDLRV
AEVKEAEKIEGSNKLLKLKVSLGDEERQVVAGIAKHYNPEELVGKKVILVANLEPATIFGVKSEGMVLAASNDSGELTLT
TVEKDISSGSQVK
>Mature_652_residues
SEKTFYVTTPIYYPSDRLHIGHAYTTVAADTLAKFKEMQDYDTMFLTGSDEHGQKIERKASEAGKKPGEYVDEIVATFKD
LWKRLGVDYDYFIRTTNPDHEKVVQEIFKKIYEKGDIYKGKYEGWYCTPCETFWTERQLEGEKVCPDCGRPVEWVEEESY
FFKLSKYADRLLEHIKKHPEFIQPESRRKEMINFIESGLEDLSVSRTTFDWGIPVPIDDDHVIYVWFDALSNYISAIGYL
SDDKKFNHYWPADVHIVGKDILRFHTIIWPIILMALDLPLPKKVFGHGWLLTETGKMSKSKGNVVDPNKLIDDFGVDPIR
YYLLREVAFGQDGTYSTEALIKRINSDLANDLGNLLHRTVTMVDKYFDGVIPEPGVEKEVDKDLKNYALKTLDEIDNSMD
NLKYAVALESLWNFIRRSNKYIDQTTPWILARDEDKKDRLGTVLYNLIEALRIIAGMLKPFLVETPYMIGEQINARDLIE
KASWSDLKQWGLLSPGRKVKKGKPIYPRIDIEEYFERLNNNENKETSDNKGKGGNDMAEQDKNTGTISFNDFTKLDLRVA
EVKEAEKIEGSNKLLKLKVSLGDEERQVVAGIAKHYNPEELVGKKVILVANLEPATIFGVKSEGMVLAASNDSGELTLTT
VEKDISSGSQVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=483, Percent_Identity=36.4389233954451, Blast_Score=318, Evalue=1e-86,
Organism=Homo sapiens, GI14043022, Length=577, Percent_Identity=26.6897746967071, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI7661872, Length=183, Percent_Identity=27.3224043715847, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1788432, Length=692, Percent_Identity=27.7456647398844, Blast_Score=275, Evalue=8e-75,
Organism=Escherichia coli, GI1786861, Length=179, Percent_Identity=28.4916201117318, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1789455, Length=107, Percent_Identity=35.5140186915888, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI212645096, Length=483, Percent_Identity=35.4037267080745, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI71992781, Length=377, Percent_Identity=35.0132625994695, Blast_Score=203, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17540794, Length=580, Percent_Identity=24.4827586206897, Blast_Score=181, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI71997510, Length=177, Percent_Identity=25.9887005649718, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6321610, Length=515, Percent_Identity=32.0388349514563, Blast_Score=271, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6321704, Length=564, Percent_Identity=26.4184397163121, Blast_Score=174, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6323414, Length=174, Percent_Identity=27.0114942528736, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21358483, Length=479, Percent_Identity=33.8204592901879, Blast_Score=298, Evalue=7e-81,
Organism=Drosophila melanogaster, GI24655311, Length=603, Percent_Identity=26.3681592039801, Blast_Score=197, Evalue=2e-50,
Organism=Drosophila melanogaster, GI21355409, Length=178, Percent_Identity=26.4044943820225, Blast_Score=76, Evalue=8e-14,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 74891; Mature: 74760

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKTFYVTTPIYYPSDRLHIGHAYTTVAADTLAKFKEMQDYDTMFLTGSDEHGQKIERK
CCCCCEEEECCEECCCCCEEECHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
ASEAGKKPGEYVDEIVATFKDLWKRLGVDYDYFIRTTNPDHEKVVQEIFKKIYEKGDIYK
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCC
GKYEGWYCTPCETFWTERQLEGEKVCPDCGRPVEWVEEESYFFKLSKYADRLLEHIKKHP
CCCCCEEECCHHHHHHHHHCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCC
EFIQPESRRKEMINFIESGLEDLSVSRTTFDWGIPVPIDDDHVIYVWFDALSNYISAIGY
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHC
LSDDKKFNHYWPADVHIVGKDILRFHTIIWPIILMALDLPLPKKVFGHGWLLTETGKMSK
CCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEECCCCCCC
SKGNVVDPNKLIDDFGVDPIRYYLLREVAFGQDGTYSTEALIKRINSDLANDLGNLLHRT
CCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VTMVDKYFDGVIPEPGVEKEVDKDLKNYALKTLDEIDNSMDNLKYAVALESLWNFIRRSN
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KYIDQTTPWILARDEDKKDRLGTVLYNLIEALRIIAGMLKPFLVETPYMIGEQINARDLI
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHH
EKASWSDLKQWGLLSPGRKVKKGKPIYPRIDIEEYFERLNNNENKETSDNKGKGGNDMAE
HHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
QDKNTGTISFNDFTKLDLRVAEVKEAEKIEGSNKLLKLKVSLGDEERQVVAGIAKHYNPE
CCCCCCEEEECCCCCHHEEHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCHH
ELVGKKVILVANLEPATIFGVKSEGMVLAASNDSGELTLTTVEKDISSGSQVK
HHCCCEEEEEEECCCCEEEEECCCCEEEEECCCCCCEEEEEEHHHHCCCCCCC
>Mature Secondary Structure 
SEKTFYVTTPIYYPSDRLHIGHAYTTVAADTLAKFKEMQDYDTMFLTGSDEHGQKIERK
CCCCEEEECCEECCCCCEEECHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
ASEAGKKPGEYVDEIVATFKDLWKRLGVDYDYFIRTTNPDHEKVVQEIFKKIYEKGDIYK
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCC
GKYEGWYCTPCETFWTERQLEGEKVCPDCGRPVEWVEEESYFFKLSKYADRLLEHIKKHP
CCCCCEEECCHHHHHHHHHCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCC
EFIQPESRRKEMINFIESGLEDLSVSRTTFDWGIPVPIDDDHVIYVWFDALSNYISAIGY
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHC
LSDDKKFNHYWPADVHIVGKDILRFHTIIWPIILMALDLPLPKKVFGHGWLLTETGKMSK
CCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEECCCCCCC
SKGNVVDPNKLIDDFGVDPIRYYLLREVAFGQDGTYSTEALIKRINSDLANDLGNLLHRT
CCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VTMVDKYFDGVIPEPGVEKEVDKDLKNYALKTLDEIDNSMDNLKYAVALESLWNFIRRSN
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KYIDQTTPWILARDEDKKDRLGTVLYNLIEALRIIAGMLKPFLVETPYMIGEQINARDLI
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHH
EKASWSDLKQWGLLSPGRKVKKGKPIYPRIDIEEYFERLNNNENKETSDNKGKGGNDMAE
HHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
QDKNTGTISFNDFTKLDLRVAEVKEAEKIEGSNKLLKLKVSLGDEERQVVAGIAKHYNPE
CCCCCCEEEECCCCCHHEEHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCHH
ELVGKKVILVANLEPATIFGVKSEGMVLAASNDSGELTLTTVEKDISSGSQVK
HHCCCEEEEEEECCCCEEEEECCCCEEEEECCCCCCEEEEEEHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]