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The map label for this gene is thiH [H]

Identifier: 220932766

GI number: 220932766

Start: 2069224

End: 2070669

Strand: Reverse

Name: thiH [H]

Synonym: Hore_19320

Alternate gene names: 220932766

Gene position: 2070669-2069224 (Counterclockwise)

Preceding gene: 220932767

Following gene: 220932765

Centisome position: 80.32

GC content: 43.91

Gene sequence:

>1446_bases
ATGGGCAGTTTATCATTTCCAGATCATCGCAGAGGGGAGTCGAGGAGTAAAGTCTGTGAATACATTAATGGGGATAAAAT
CGAGGCCATTCTGGAGGAGGCCAGTAATCCTTCAGAAGAGGAAGTTAACCACATTATAAAAAAGTCCCTGGAGCTCAAGG
GACTATCAGTAGAAGAAGCAGCTGTATTGCTCCAGGTAGAGGACCAGGAATTAATCAATAAATTCCTGGAAGCAGCCAGA
AAGGTGAAAGAAAAGATTTACGGGAAAAGGCTTGTTCTTTTTGCTCCCCTGTATTTTTCGAATCTATGTACTAATAGCTG
TCTTTACTGTAGCTTCAGGCATAATAACAATAAAGTTAAAAGAAAAAAACTCAGTATAGAAGAGATTAAGGAAGAAGTCA
GAGCCCTGGAAAGAGAAGGACATAAACGCCTGCTTGTTTTGACCGGGGAAACCCCCGAAACGGACCTTGATTATGTGGTT
GAAGGTATTAAAGCAGCTTATGAAACCAGGACTGAACATGGTGGTGAGATCAGGAGGATAAATGTAGAGATTGCCCCCCT
GACCACAGAAGATTTCAAAAAACTCAAGGAAGCTAAAATTGGAACCTATACCTGTTTTCAGGAGACATATCACCGTCCTA
CCTATAAGAAAATGCATCCATCTGGCCCCAAAGCCGATTATGACTGGCGGTTGTCAGTTATGGACCGGGCCCAGCAGGCC
GGTATTGATGATATAGGTATCGGGGCTCTCTTTGGACTGTATGACTATAAATTTGAGGTTATTGCCCTGTTACTACATTC
TGAATACCTGGATAAGACCTATGGTGTTGGCCCCCATACAATATCGGTTCCCCGTTTAAACCCCGCCCTGGGGGCACCTG
TCCAGGAGCCACCATATCCTGTGTCTGATGAGGATTTCAGGAAACTTGTAGCAATCTTGAGGCTGGCTGTTCCCTATACA
GGGATAATTTTATCAACCAGGGAGAGTATTGACATGCGTAATGAATTGTTTTTGCACGGAGTTTCCCAGATAAGTGCCGG
ATCCCGGACTACTCCAGGGGGGTATAGAGAGGCCCGGGAACGGGAGCATGATCTGGAGCAATTTTCTCTCCATGACATCA
GGCCCATGGATGAAATTATAGCTGAAATTAGCAAACAGGGGTATATACCGAGTTTCTGTACTGCCTGTTACCGCCTGGGG
CGGACCGGTCAGGATTTTATGGACCTTGCTAAACCGGGTAAGATTCAGGAATTTTGTAAACCCAATGCCATGTTTACCTT
TAAAGAATACCTGGTTGACTACGCCAGTCCTGAAACACGCAAACTGGGTGAGGAGTGTTTACAGGCTCATTTAAAAGAAA
TTAAAGATCTAAATCCCATGCTGGCTCAAAAAGTGAAGACAAATCTTACTAAAATAGAAAATGGTGAACATGACCTGTAC
TTTTAA

Upstream 100 bases:

>100_bases
AACTCAGGGTAAGGATAGTCATTAAGGCCAGTAATCACCAGGTGAGGTTTGTGTTTAAGGTAAAGGGAAATTAAAAAATT
TGAAGGGGGATTGTTTATCT

Downstream 100 bases:

>100_bases
TCCAGGGGCTTTTATAAATGATTTTCTGTTAACAAAGTTGGCTTATGACTTTCTTTCAATATCACTTCAATTTATTTACG
ATGGATTGGGGATTAAGCAT

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MGSLSFPDHRRGESRSKVCEYINGDKIEAILEEASNPSEEEVNHIIKKSLELKGLSVEEAAVLLQVEDQELINKFLEAAR
KVKEKIYGKRLVLFAPLYFSNLCTNSCLYCSFRHNNNKVKRKKLSIEEIKEEVRALEREGHKRLLVLTGETPETDLDYVV
EGIKAAYETRTEHGGEIRRINVEIAPLTTEDFKKLKEAKIGTYTCFQETYHRPTYKKMHPSGPKADYDWRLSVMDRAQQA
GIDDIGIGALFGLYDYKFEVIALLLHSEYLDKTYGVGPHTISVPRLNPALGAPVQEPPYPVSDEDFRKLVAILRLAVPYT
GIILSTRESIDMRNELFLHGVSQISAGSRTTPGGYREAREREHDLEQFSLHDIRPMDEIIAEISKQGYIPSFCTACYRLG
RTGQDFMDLAKPGKIQEFCKPNAMFTFKEYLVDYASPETRKLGEECLQAHLKEIKDLNPMLAQKVKTNLTKIENGEHDLY
F

Sequences:

>Translated_481_residues
MGSLSFPDHRRGESRSKVCEYINGDKIEAILEEASNPSEEEVNHIIKKSLELKGLSVEEAAVLLQVEDQELINKFLEAAR
KVKEKIYGKRLVLFAPLYFSNLCTNSCLYCSFRHNNNKVKRKKLSIEEIKEEVRALEREGHKRLLVLTGETPETDLDYVV
EGIKAAYETRTEHGGEIRRINVEIAPLTTEDFKKLKEAKIGTYTCFQETYHRPTYKKMHPSGPKADYDWRLSVMDRAQQA
GIDDIGIGALFGLYDYKFEVIALLLHSEYLDKTYGVGPHTISVPRLNPALGAPVQEPPYPVSDEDFRKLVAILRLAVPYT
GIILSTRESIDMRNELFLHGVSQISAGSRTTPGGYREAREREHDLEQFSLHDIRPMDEIIAEISKQGYIPSFCTACYRLG
RTGQDFMDLAKPGKIQEFCKPNAMFTFKEYLVDYASPETRKLGEECLQAHLKEIKDLNPMLAQKVKTNLTKIENGEHDLY
F
>Mature_480_residues
GSLSFPDHRRGESRSKVCEYINGDKIEAILEEASNPSEEEVNHIIKKSLELKGLSVEEAAVLLQVEDQELINKFLEAARK
VKEKIYGKRLVLFAPLYFSNLCTNSCLYCSFRHNNNKVKRKKLSIEEIKEEVRALEREGHKRLLVLTGETPETDLDYVVE
GIKAAYETRTEHGGEIRRINVEIAPLTTEDFKKLKEAKIGTYTCFQETYHRPTYKKMHPSGPKADYDWRLSVMDRAQQAG
IDDIGIGALFGLYDYKFEVIALLLHSEYLDKTYGVGPHTISVPRLNPALGAPVQEPPYPVSDEDFRKLVAILRLAVPYTG
IILSTRESIDMRNELFLHGVSQISAGSRTTPGGYREAREREHDLEQFSLHDIRPMDEIIAEISKQGYIPSFCTACYRLGR
TGQDFMDLAKPGKIQEFCKPNAMFTFKEYLVDYASPETRKLGEECLQAHLKEIKDLNPMLAQKVKTNLTKIENGEHDLYF

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=361, Percent_Identity=31.5789473684211, Blast_Score=191, Evalue=9e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 54893; Mature: 54762

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSLSFPDHRRGESRSKVCEYINGDKIEAILEEASNPSEEEVNHIIKKSLELKGLSVEEA
CCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHH
AVLLQVEDQELINKFLEAARKVKEKIYGKRLVLFAPLYFSNLCTNSCLYCSFRHNNNKVK
EEEEEECHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHCCCEEEEEEECCCCHHH
RKKLSIEEIKEEVRALEREGHKRLLVLTGETPETDLDYVVEGIKAAYETRTEHGGEIRRI
HHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
NVEIAPLTTEDFKKLKEAKIGTYTCFQETYHRPTYKKMHPSGPKADYDWRLSVMDRAQQA
EEEEECCCHHHHHHHHHHCCCHHHHHHHHHCCCCHHCCCCCCCCCCCCEEHHHHHHHHHC
GIDDIGIGALFGLYDYKFEVIALLLHSEYLDKTYGVGPHTISVPRLNPALGAPVQEPPYP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCC
VSDEDFRKLVAILRLAVPYTGIILSTRESIDMRNELFLHGVSQISAGSRTTPGGYREARE
CCHHHHHHHHHHHHHHCCHHCEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
REHDLEQFSLHDIRPMDEIIAEISKQGYIPSFCTACYRLGRTGQDFMDLAKPGKIQEFCK
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHC
PNAMFTFKEYLVDYASPETRKLGEECLQAHLKEIKDLNPMLAQKVKTNLTKIENGEHDLY
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCC
F
C
>Mature Secondary Structure 
GSLSFPDHRRGESRSKVCEYINGDKIEAILEEASNPSEEEVNHIIKKSLELKGLSVEEA
CCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHH
AVLLQVEDQELINKFLEAARKVKEKIYGKRLVLFAPLYFSNLCTNSCLYCSFRHNNNKVK
EEEEEECHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHCCCEEEEEEECCCCHHH
RKKLSIEEIKEEVRALEREGHKRLLVLTGETPETDLDYVVEGIKAAYETRTEHGGEIRRI
HHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
NVEIAPLTTEDFKKLKEAKIGTYTCFQETYHRPTYKKMHPSGPKADYDWRLSVMDRAQQA
EEEEECCCHHHHHHHHHHCCCHHHHHHHHHCCCCHHCCCCCCCCCCCCEEHHHHHHHHHC
GIDDIGIGALFGLYDYKFEVIALLLHSEYLDKTYGVGPHTISVPRLNPALGAPVQEPPYP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCC
VSDEDFRKLVAILRLAVPYTGIILSTRESIDMRNELFLHGVSQISAGSRTTPGGYREARE
CCHHHHHHHHHHHHHHCCHHCEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
REHDLEQFSLHDIRPMDEIIAEISKQGYIPSFCTACYRLGRTGQDFMDLAKPGKIQEFCK
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHC
PNAMFTFKEYLVDYASPETRKLGEECLQAHLKEIKDLNPMLAQKVKTNLTKIENGEHDLY
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]