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The map label for this gene is mnmE [H]

Identifier: 220932765

GI number: 220932765

Start: 2067760

End: 2068995

Strand: Reverse

Name: mnmE [H]

Synonym: Hore_19310

Alternate gene names: 220932765

Gene position: 2068995-2067760 (Counterclockwise)

Preceding gene: 220932766

Following gene: 220932764

Centisome position: 80.25

GC content: 44.66

Gene sequence:

>1236_bases
ATGCAGCAGACACCCAGGGCCAATCGTCCTCATATAGCAGTCTTCGGCCGCCGTAATGTGGGGAAATCAACCCTGATTAA
TACCCTGACCAATCAGGAACTGGCTTTAGTATCTGATGTGCCGGGAACAACAACTGACCCGGTTTATAAATCTATGGAGT
TACTGCCCCTGGGCCCCGTTGTTATGATAGATACAGCCGGGATTGATGATGTCGGTTCCCTGGGGAAATTAAGGATAAAA
AAAACCAGGGAAGTTATCAGGAGAACTGACCTGGCCATTCTGGTTATAGATCCGTTCCATGGAGCAGGCAATTACGAGAA
GGATTTATATAATAAATTGCAGGATAATAATATTCCGGTTGTAGTAGTCATAAATAAGATTGACCGGGTGAAGGGTATAA
AACAGGATTTATTAGATAAGGTAAAGTTATTATTCGGTGTAACCCCCATTAAGGTCAGTGCCCACTCAGGGACAGGAATA
GAAGAACTCCGGGAGGTCCTTGTAAACAGGGTTCCCAGAGACCATGAACAGCCCCATATTATCGGTGATTTGATAGATGA
AGGTGATACCGTAATCCTGGTTACCCCTATTGACAGTGCTGCTCCGAAGGGAAGGTTAATCCTGCCCCAGGTCCAGACCC
TGAGGGATATTCTCGATAATCATGGGATGGGCCTGGTTGTTAAAGAAACAGAGTTAGAGGGGGCTTTAAAAACACTCAGG
GAAAAACCTAGATTGGTGGTTACCGATTCCCAGGTCTTTGGCCTGGTAAGTAAGATTGTGCCTGAAGACATCTATTTAAC
CGGTTTTTCTATTCTCTTTGCCCGTTATAAAGGGGATTTAATGAAATATCTGGAAGGGGTTGCGAAACTGGAGAATCTCA
GGCCCGGGGATAAGATTCTAATAGCAGAGGCCTGTACCCATCGCCGTCAGCCAGATGACATCGGAACTGTTAAACTTCCG
GGATGGATCAGGTCAAGGATTTGTAATGAGGTTAAATTTGACCTTGTTTCGGGACGGGAATATCCCGATAACCTCGAACA
GTATGATCTGGTATTACACTGTGCGGGATGTATGCTCAATAGAAAAGAGGTTTTATCCAGGTTGAAAGAAGCAAATACAT
CTGGGGTACCTGTTGTAAACTATGGTATGGCTATTGCTTATCTCCATGGTATTCTGGACAGGGCTTTAAAACCTTTTGGT
GAAGCCTATCAGACGTGGAAGGGTTATAGAAAGTGA

Upstream 100 bases:

>100_bases
TTTAATGAAATCTTTAATTTTTTAAACGCAATGTTGGTTGGAGAATTTAAGCGTGTATCAGTAATTTATTATTTTTAAAG
AGAGGAGTGTTAGGTTAGAT

Downstream 100 bases:

>100_bases
TTTAATTTTAATTTTTATTTAAAAATTCCTTGCATAAATGAATAATTGCTGTTATACTAAATATAAATTAAAAAATAGTA
AATTAAATTTTATTCTAAAA

Product: small GTP-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MQQTPRANRPHIAVFGRRNVGKSTLINTLTNQELALVSDVPGTTTDPVYKSMELLPLGPVVMIDTAGIDDVGSLGKLRIK
KTREVIRRTDLAILVIDPFHGAGNYEKDLYNKLQDNNIPVVVVINKIDRVKGIKQDLLDKVKLLFGVTPIKVSAHSGTGI
EELREVLVNRVPRDHEQPHIIGDLIDEGDTVILVTPIDSAAPKGRLILPQVQTLRDILDNHGMGLVVKETELEGALKTLR
EKPRLVVTDSQVFGLVSKIVPEDIYLTGFSILFARYKGDLMKYLEGVAKLENLRPGDKILIAEACTHRRQPDDIGTVKLP
GWIRSRICNEVKFDLVSGREYPDNLEQYDLVLHCAGCMLNRKEVLSRLKEANTSGVPVVNYGMAIAYLHGILDRALKPFG
EAYQTWKGYRK

Sequences:

>Translated_411_residues
MQQTPRANRPHIAVFGRRNVGKSTLINTLTNQELALVSDVPGTTTDPVYKSMELLPLGPVVMIDTAGIDDVGSLGKLRIK
KTREVIRRTDLAILVIDPFHGAGNYEKDLYNKLQDNNIPVVVVINKIDRVKGIKQDLLDKVKLLFGVTPIKVSAHSGTGI
EELREVLVNRVPRDHEQPHIIGDLIDEGDTVILVTPIDSAAPKGRLILPQVQTLRDILDNHGMGLVVKETELEGALKTLR
EKPRLVVTDSQVFGLVSKIVPEDIYLTGFSILFARYKGDLMKYLEGVAKLENLRPGDKILIAEACTHRRQPDDIGTVKLP
GWIRSRICNEVKFDLVSGREYPDNLEQYDLVLHCAGCMLNRKEVLSRLKEANTSGVPVVNYGMAIAYLHGILDRALKPFG
EAYQTWKGYRK
>Mature_411_residues
MQQTPRANRPHIAVFGRRNVGKSTLINTLTNQELALVSDVPGTTTDPVYKSMELLPLGPVVMIDTAGIDDVGSLGKLRIK
KTREVIRRTDLAILVIDPFHGAGNYEKDLYNKLQDNNIPVVVVINKIDRVKGIKQDLLDKVKLLFGVTPIKVSAHSGTGI
EELREVLVNRVPRDHEQPHIIGDLIDEGDTVILVTPIDSAAPKGRLILPQVQTLRDILDNHGMGLVVKETELEGALKTLR
EKPRLVVTDSQVFGLVSKIVPEDIYLTGFSILFARYKGDLMKYLEGVAKLENLRPGDKILIAEACTHRRQPDDIGTVKLP
GWIRSRICNEVKFDLVSGREYPDNLEQYDLVLHCAGCMLNRKEVLSRLKEANTSGVPVVNYGMAIAYLHGILDRALKPFG
EAYQTWKGYRK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG1160

COG function: function code R; Predicted GTPases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Escherichia coli, GI87082120, Length=166, Percent_Identity=34.3373493975904, Blast_Score=72, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323665, Length=181, Percent_Identity=30.939226519337, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 45814; Mature: 45814

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQTPRANRPHIAVFGRRNVGKSTLINTLTNQELALVSDVPGTTTDPVYKSMELLPLGPV
CCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCCEECCCCE
VMIDTAGIDDVGSLGKLRIKKTREVIRRTDLAILVIDPFHGAGNYEKDLYNKLQDNNIPV
EEEECCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCE
VVVINKIDRVKGIKQDLLDKVKLLFGVTPIKVSAHSGTGIEELREVLVNRVPRDHEQPHI
EEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCC
IGDLIDEGDTVILVTPIDSAAPKGRLILPQVQTLRDILDNHGMGLVVKETELEGALKTLR
HHHHHCCCCEEEEEECCCCCCCCCCEECCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHH
EKPRLVVTDSQVFGLVSKIVPEDIYLTGFSILFARYKGDLMKYLEGVAKLENLRPGDKIL
HCCCEEEECHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
IAEACTHRRQPDDIGTVKLPGWIRSRICNEVKFDLVSGREYPDNLEQYDLVLHCAGCMLN
EEEHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
RKEVLSRLKEANTSGVPVVNYGMAIAYLHGILDRALKPFGEAYQTWKGYRK
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQQTPRANRPHIAVFGRRNVGKSTLINTLTNQELALVSDVPGTTTDPVYKSMELLPLGPV
CCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCCEECCCCE
VMIDTAGIDDVGSLGKLRIKKTREVIRRTDLAILVIDPFHGAGNYEKDLYNKLQDNNIPV
EEEECCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCE
VVVINKIDRVKGIKQDLLDKVKLLFGVTPIKVSAHSGTGIEELREVLVNRVPRDHEQPHI
EEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCC
IGDLIDEGDTVILVTPIDSAAPKGRLILPQVQTLRDILDNHGMGLVVKETELEGALKTLR
HHHHHCCCCEEEEEECCCCCCCCCCEECCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHH
EKPRLVVTDSQVFGLVSKIVPEDIYLTGFSILFARYKGDLMKYLEGVAKLENLRPGDKIL
HCCCEEEECHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
IAEACTHRRQPDDIGTVKLPGWIRSRICNEVKFDLVSGREYPDNLEQYDLVLHCAGCMLN
EEEHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
RKEVLSRLKEANTSGVPVVNYGMAIAYLHGILDRALKPFGEAYQTWKGYRK
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA