Click here to switch to the map view.

The map label for this gene is hisJ [H]

Identifier: 220932713

GI number: 220932713

Start: 2005123

End: 2005857

Strand: Reverse

Name: hisJ [H]

Synonym: Hore_18770

Alternate gene names: 220932713

Gene position: 2005857-2005123 (Counterclockwise)

Preceding gene: 220932715

Following gene: 220932712

Centisome position: 77.8

GC content: 36.87

Gene sequence:

>735_bases
ATGAAAAAATTATCAATTATATTAGTAACACTGTTACTGGTAATGCTGGCAGGGATAGGTGTGTCTGCTGAAAAATATGT
AGTGGGGACCAGTGCTGATTTTCCACCATTTGAGTATGTTGAAGACGGGGAATTTGTTGGTTTTGACATGGATTTAATAA
GGGCCATTGCAGAGGTTGAGGGTTTTGAAGTAGAGATTAGAGATATGAGTTTTGATTCCCTGATTGCAGCCTTGAAAAGC
GGTAATATTGATATTGCAATTGCCGGTATGACCATAACTGAAAAGAGGGAAAAGGTTGTTGATTTCTCTATTCCTTACTA
TACGGCAAACCAGAGTGTAATTGTTAAAGAGGATTCTGACCTTAATTTAACAGTGCTTTACGGTGACAATAATGTTGGGG
TTCAGACCAGTACTACCGGGGATTTATGGGTAACAGATAATTTAGAAAAGAAAGGTATTTTAACCGGTAAAGTAGTAAGA
TATGACACCTTTGTTCTGGCTGTAACTGACCTGGTTAATGGAAACCTTGATGCTATTGTCCTTGATAGCCCTGTGGCTGA
AAGGTTTGTGGATAGCCATCCCCTGAAGGAAGTTGGAATAATAATTACAGGTGAAGAGTATGGTATTGTCGTTAATGAGG
GTAATAAGGACCTTTTAAATAAAATAAATAACGGAATTAAAAAATTACAGGAATCTGGTGTGATATCTAAACTGGTTGGT
AAGTATTTTAAATAA

Upstream 100 bases:

>100_bases
CTTGCATAACTATAGATTTTCATGTATACTATAGTTTAATAGAAAATCAGTTGCAACTGTTTTTTAGAAAGAGTTTTTAA
ATAAACAAGGGGGTTTTTTA

Downstream 100 bases:

>100_bases
GATTTATATATCAAGGGTCCTCTCATGATTAAATTTTCCGGTTATATAAAAATGAGCAACTGGATTAGCATCGAGGCCAT
TGAGCCTCCTTGCGGTTACA

Product: family 3 extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MKKLSIILVTLLLVMLAGIGVSAEKYVVGTSADFPPFEYVEDGEFVGFDMDLIRAIAEVEGFEVEIRDMSFDSLIAALKS
GNIDIAIAGMTITEKREKVVDFSIPYYTANQSVIVKEDSDLNLTVLYGDNNVGVQTSTTGDLWVTDNLEKKGILTGKVVR
YDTFVLAVTDLVNGNLDAIVLDSPVAERFVDSHPLKEVGIIITGEEYGIVVNEGNKDLLNKINNGIKKLQESGVISKLVG
KYFK

Sequences:

>Translated_244_residues
MKKLSIILVTLLLVMLAGIGVSAEKYVVGTSADFPPFEYVEDGEFVGFDMDLIRAIAEVEGFEVEIRDMSFDSLIAALKS
GNIDIAIAGMTITEKREKVVDFSIPYYTANQSVIVKEDSDLNLTVLYGDNNVGVQTSTTGDLWVTDNLEKKGILTGKVVR
YDTFVLAVTDLVNGNLDAIVLDSPVAERFVDSHPLKEVGIIITGEEYGIVVNEGNKDLLNKINNGIKKLQESGVISKLVG
KYFK
>Mature_244_residues
MKKLSIILVTLLLVMLAGIGVSAEKYVVGTSADFPPFEYVEDGEFVGFDMDLIRAIAEVEGFEVEIRDMSFDSLIAALKS
GNIDIAIAGMTITEKREKVVDFSIPYYTANQSVIVKEDSDLNLTVLYGDNNVGVQTSTTGDLWVTDNLEKKGILTGKVVR
YDTFVLAVTDLVNGNLDAIVLDSPVAERFVDSHPLKEVGIIITGEEYGIVVNEGNKDLLNKINNGIKKLQESGVISKLVG
KYFK

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=252, Percent_Identity=34.1269841269841, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1787031, Length=243, Percent_Identity=31.6872427983539, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1787088, Length=251, Percent_Identity=31.8725099601594, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1788228, Length=223, Percent_Identity=29.5964125560538, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788649, Length=252, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1788648, Length=228, Percent_Identity=28.5087719298246, Blast_Score=84, Evalue=9e-18,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 26663; Mature: 26663

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLSIILVTLLLVMLAGIGVSAEKYVVGTSADFPPFEYVEDGEFVGFDMDLIRAIAEVE
CCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECHHHHHHHHHCC
GFEVEIRDMSFDSLIAALKSGNIDIAIAGMTITEKREKVVDFSIPYYTANQSVIVKEDSD
CCEEEEECCCHHHHHHHHHCCCEEEEEEEEEEECCCCEEEEEECCEEECCCEEEEEECCC
LNLTVLYGDNNVGVQTSTTGDLWVTDNLEKKGILTGKVVRYDTFVLAVTDLVNGNLDAIV
CEEEEEECCCCCCEEECCCCCEEEECCCCCCCCEECEEEEEEEEEEEEEHHHCCCCCEEE
LDSPVAERFVDSHPLKEVGIIITGEEYGIVVNEGNKDLLNKINNGIKKLQESGVISKLVG
ECCHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHH
KYFK
HHCC
>Mature Secondary Structure
MKKLSIILVTLLLVMLAGIGVSAEKYVVGTSADFPPFEYVEDGEFVGFDMDLIRAIAEVE
CCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECHHHHHHHHHCC
GFEVEIRDMSFDSLIAALKSGNIDIAIAGMTITEKREKVVDFSIPYYTANQSVIVKEDSD
CCEEEEECCCHHHHHHHHHCCCEEEEEEEEEEECCCCEEEEEECCEEECCCEEEEEECCC
LNLTVLYGDNNVGVQTSTTGDLWVTDNLEKKGILTGKVVRYDTFVLAVTDLVNGNLDAIV
CEEEEEECCCCCCEEECCCCCEEEECCCCCCCCEECEEEEEEEEEEEEEHHHCCCCCEEE
LDSPVAERFVDSHPLKEVGIIITGEEYGIVVNEGNKDLLNKINNGIKKLQESGVISKLVG
ECCHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHH
KYFK
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1448614 [H]