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The map label for this gene is fliM [H]

Identifier: 220932512

GI number: 220932512

Start: 1797428

End: 1798432

Strand: Reverse

Name: fliM [H]

Synonym: Hore_16750

Alternate gene names: 220932512

Gene position: 1798432-1797428 (Counterclockwise)

Preceding gene: 220932513

Following gene: 220932511

Centisome position: 69.76

GC content: 42.09

Gene sequence:

>1005_bases
ATGGCTGATGTATTGAGTCAGAGTGAAATAGATTCGTTACTATCTGCCCTGTCATCCGGTGATGTGGATGTCGAGGAGAT
AAAAAAAGAGACAGAAGAAGAAAAGGTAAAGGTTTATGATTTTCGGCGCCCCGATAAGCTGTCTAAAGAACAGATGAGAA
CCCTGGAAATGATCCATGAAAACTTTGCCCGAATCCTGACCACTACCTTATCCACCCAGCTCAGGAGTAGAGTCGATTTT
GAGGTGGCTTCTATTGAACAGTTAACCTATGAAGAATTTATCCGGTCTCTACCAGAACCGACCATTATCGGTATCAGCGA
GATGGAGCCCTTTAACGGACAGTTTATCTTTGAAATAAACCCTGATATTGGTTTTGCTATCATTGACAGGTTATTCGGGG
GAATGGGGAATCCCCTGGACAGGGTCAGGACCTATACAGATATTGAAAAGGTTGTTTTACGAAAGGTAATATCCTGGTTT
TTGAGTGGTTTACCGGAAGCCTGGGAAAACATTATCAGGGTTAAACCGAGGTTAAGGGAAATAGAGTCCAACCCTCAGTT
TACCCAGGTTGTTCCCAGTAATGATATGACTATCCTGATTACCATGTCAGCCAAGATAGCAAATAGTGAAGGTCTGGTAA
ATGTCTGTATTCCCTACATCATGATAGAACCCATTGTACCACAATTAAATGCCCAGCAGTGGTTTTCCAATACCAGGGAA
GAAAGAACAGCCCAGCATATTGAGGCTATCAAGAAGAAACTTAAGCGGGCCCCTCTTGAGGTTATTGCTGAAGTAGGGAA
AACCCAGCTTACTGTTACTGATTTACTATATCTTCAACCCGGTGATGTCATCAGGCTAGACAAAAAGGCCGAAGAAAAGG
TTGATATACGTATCGGGAAAAACGTTAAATTTCAGGGGGTTGCCGGGGTTAGTAATAAACATATGGCTGTAAAAATTACT
GATGTTATCAGTAATGAAGAGGAAGGTGAAGAGAATGATGGATAA

Upstream 100 bases:

>100_bases
AAAAAATAGAGTCAGGGCTGAGTTAAATCAGGTTTTAACTACCGGGAAAATCACCAGTGTTTGGTTTACTCAGCTGGTGG
TTCAGTAGGAGGTTAATCAT

Downstream 100 bases:

>100_bases
CCAGGATAAAGATATACTATCCCAGGAAGAAATTGATGCCCTTATGGATAACAGTGATGAGGATAGCAATGAGTTAACCC
ATGAGGAAAGGGATGTTCTG

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MADVLSQSEIDSLLSALSSGDVDVEEIKKETEEEKVKVYDFRRPDKLSKEQMRTLEMIHENFARILTTTLSTQLRSRVDF
EVASIEQLTYEEFIRSLPEPTIIGISEMEPFNGQFIFEINPDIGFAIIDRLFGGMGNPLDRVRTYTDIEKVVLRKVISWF
LSGLPEAWENIIRVKPRLREIESNPQFTQVVPSNDMTILITMSAKIANSEGLVNVCIPYIMIEPIVPQLNAQQWFSNTRE
ERTAQHIEAIKKKLKRAPLEVIAEVGKTQLTVTDLLYLQPGDVIRLDKKAEEKVDIRIGKNVKFQGVAGVSNKHMAVKIT
DVISNEEEGEENDG

Sequences:

>Translated_334_residues
MADVLSQSEIDSLLSALSSGDVDVEEIKKETEEEKVKVYDFRRPDKLSKEQMRTLEMIHENFARILTTTLSTQLRSRVDF
EVASIEQLTYEEFIRSLPEPTIIGISEMEPFNGQFIFEINPDIGFAIIDRLFGGMGNPLDRVRTYTDIEKVVLRKVISWF
LSGLPEAWENIIRVKPRLREIESNPQFTQVVPSNDMTILITMSAKIANSEGLVNVCIPYIMIEPIVPQLNAQQWFSNTRE
ERTAQHIEAIKKKLKRAPLEVIAEVGKTQLTVTDLLYLQPGDVIRLDKKAEEKVDIRIGKNVKFQGVAGVSNKHMAVKIT
DVISNEEEGEENDG
>Mature_333_residues
ADVLSQSEIDSLLSALSSGDVDVEEIKKETEEEKVKVYDFRRPDKLSKEQMRTLEMIHENFARILTTTLSTQLRSRVDFE
VASIEQLTYEEFIRSLPEPTIIGISEMEPFNGQFIFEINPDIGFAIIDRLFGGMGNPLDRVRTYTDIEKVVLRKVISWFL
SGLPEAWENIIRVKPRLREIESNPQFTQVVPSNDMTILITMSAKIANSEGLVNVCIPYIMIEPIVPQLNAQQWFSNTREE
RTAQHIEAIKKKLKRAPLEVIAEVGKTQLTVTDLLYLQPGDVIRLDKKAEEKVDIRIGKNVKFQGVAGVSNKHMAVKITD
VISNEEEGEENDG

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=328, Percent_Identity=28.6585365853659, Blast_Score=132, Evalue=3e-32,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 37979; Mature: 37848

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADVLSQSEIDSLLSALSSGDVDVEEIKKETEEEKVKVYDFRRPDKLSKEQMRTLEMIHE
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHH
NFARILTTTLSTQLRSRVDFEVASIEQLTYEEFIRSLPEPTIIGISEMEPFNGQFIFEIN
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEC
PDIGFAIIDRLFGGMGNPLDRVRTYTDIEKVVLRKVISWFLSGLPEAWENIIRVKPRLRE
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHH
IESNPQFTQVVPSNDMTILITMSAKIANSEGLVNVCIPYIMIEPIVPQLNAQQWFSNTRE
HCCCCCEEEEECCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
ERTAQHIEAIKKKLKRAPLEVIAEVGKTQLTVTDLLYLQPGDVIRLDKKAEEKVDIRIGK
HHHHHHHHHHHHHHHHCHHHHHHHCCCCEEEEHHEEEECCCCEEEECCCCCCCEEEEECC
NVKFQGVAGVSNKHMAVKITDVISNEEEGEENDG
CEEEEEECCCCCCEEEEEEHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ADVLSQSEIDSLLSALSSGDVDVEEIKKETEEEKVKVYDFRRPDKLSKEQMRTLEMIHE
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHH
NFARILTTTLSTQLRSRVDFEVASIEQLTYEEFIRSLPEPTIIGISEMEPFNGQFIFEIN
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEC
PDIGFAIIDRLFGGMGNPLDRVRTYTDIEKVVLRKVISWFLSGLPEAWENIIRVKPRLRE
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHH
IESNPQFTQVVPSNDMTILITMSAKIANSEGLVNVCIPYIMIEPIVPQLNAQQWFSNTRE
HCCCCCEEEEECCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
ERTAQHIEAIKKKLKRAPLEVIAEVGKTQLTVTDLLYLQPGDVIRLDKKAEEKVDIRIGK
HHHHHHHHHHHHHHHHCHHHHHHHCCCCEEEEHHEEEECCCCEEEECCCCCCCEEEEECC
NVKFQGVAGVSNKHMAVKITDVISNEEEGEENDG
CEEEEEECCCCCCEEEEEEHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8755894; 9665876; 8529894 [H]