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The map label for this gene is trmI [H]

Identifier: 220932460

GI number: 220932460

Start: 1745873

End: 1746640

Strand: Reverse

Name: trmI [H]

Synonym: Hore_16240

Alternate gene names: 220932460

Gene position: 1746640-1745873 (Counterclockwise)

Preceding gene: 220932461

Following gene: 220932459

Centisome position: 67.75

GC content: 39.19

Gene sequence:

>768_bases
ATGGCAGGTGGTTTAACCAATAAAAGGGTTATAACTGACCGGAAAAGGTTTAAAAAGGTTATCAGTATAGAACCGCCCCA
TAAGATAGGTTTACCAACGGGTCAGATAGATAGTAAGGTACTTAAGGATATAAGACCAGGAAGTTATTTTGAAATAAATG
GTTCCCGTTATTATGTCCTGGAGTGTGACCTTCATAACTTTATCATGAATGATTTAAAAAGGAGAACCCAGATTGTTTAC
CCCAAGGATGCTTCCTATATTATTTTTAAACTGGATATAGCTCCCGGGAAACGTATCGGTGAAGCCGGTACAGGTTCGGG
AGCTTTAACCAGTGTTTTTTCCAGGATGGTAGGACGGGATGGCCGGGTATATACATATGAAAAAAGAAAGGATTTTATAA
AAGTTATAAAGAGGAATCTGGCTCAGGTTCAGGAATTTGATAATGTTGTTCTTCACAACCAGTCTCTGGAAGATGGAATC
GAGGAAAAGGGGCTTGATGCCTTCTTCTTAGATGTAAGGGAGCCATGGCAGGTTCTTAAAAAGGTCAAATTATCCCTGAA
AGGTAATGGTCATCTGGGGTTAATACTTCCCACTACAAATCAGGTCAGTAAAACTCTTGAAGTTTTAGAGAAATTAAATT
TTTATGTATCTGAGGTGGTAGAGGTTTTATACCGGGAATACAAGCTCATACCGGAGAGGCTACGACCCGACGATACGATG
GTTGGCCATACAGGTTATTTAATATTTGCCCGTAACTTAATTGATTAA

Upstream 100 bases:

>100_bases
CCAGTATAAAGGTTATTTTTTGTTTAATTCCCGGCTCTTAAGTATTAAAGGTAGGATTTGTCCGGTGTAGTGGATATTAT
ATTATAAGGAGTGGGTGTGT

Downstream 100 bases:

>100_bases
AAACTTAGTCAATGTTGACCCCGCATAATTTAAAATAGCCGGGGTCATTTTAATTTATGAATCATATTATCTTATTTTGA
GTTTTTTTTTTTTTAAAATT

Product: tRNA methyltransferase complex GCD14 subunit

Products: NA

Alternate protein names: tRNA(m1A58)-methyltransferase; tRNA(m1A58)MTase [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MAGGLTNKRVITDRKRFKKVISIEPPHKIGLPTGQIDSKVLKDIRPGSYFEINGSRYYVLECDLHNFIMNDLKRRTQIVY
PKDASYIIFKLDIAPGKRIGEAGTGSGALTSVFSRMVGRDGRVYTYEKRKDFIKVIKRNLAQVQEFDNVVLHNQSLEDGI
EEKGLDAFFLDVREPWQVLKKVKLSLKGNGHLGLILPTTNQVSKTLEVLEKLNFYVSEVVEVLYREYKLIPERLRPDDTM
VGHTGYLIFARNLID

Sequences:

>Translated_255_residues
MAGGLTNKRVITDRKRFKKVISIEPPHKIGLPTGQIDSKVLKDIRPGSYFEINGSRYYVLECDLHNFIMNDLKRRTQIVY
PKDASYIIFKLDIAPGKRIGEAGTGSGALTSVFSRMVGRDGRVYTYEKRKDFIKVIKRNLAQVQEFDNVVLHNQSLEDGI
EEKGLDAFFLDVREPWQVLKKVKLSLKGNGHLGLILPTTNQVSKTLEVLEKLNFYVSEVVEVLYREYKLIPERLRPDDTM
VGHTGYLIFARNLID
>Mature_254_residues
AGGLTNKRVITDRKRFKKVISIEPPHKIGLPTGQIDSKVLKDIRPGSYFEINGSRYYVLECDLHNFIMNDLKRRTQIVYP
KDASYIIFKLDIAPGKRIGEAGTGSGALTSVFSRMVGRDGRVYTYEKRKDFIKVIKRNLAQVQEFDNVVLHNQSLEDGIE
EKGLDAFFLDVREPWQVLKKVKLSLKGNGHLGLILPTTNQVSKTLEVLEKLNFYVSEVVEVLYREYKLIPERLRPDDTMV
GHTGYLIFARNLID

Specific function: Catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in tRNA. Is required for cell growth at extreme temperatures [H]

COG id: COG2519

COG function: function code J; tRNA(1-methyladenosine) methyltransferase and related methyltransferases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmI family [H]

Homologues:

Organism=Homo sapiens, GI123173772, Length=224, Percent_Identity=27.2321428571429, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI222831587, Length=213, Percent_Identity=30.0469483568075, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17509459, Length=167, Percent_Identity=29.3413173652695, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6322336, Length=184, Percent_Identity=30.4347826086957, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24650257, Length=209, Percent_Identity=27.7511961722488, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014816 [H]

Pfam domain/function: PF08704 GCD14 [H]

EC number: =2.1.1.36 [H]

Molecular weight: Translated: 29189; Mature: 29058

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGGLTNKRVITDRKRFKKVISIEPPHKIGLPTGQIDSKVLKDIRPGSYFEINGSRYYVL
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEECCCEEEEE
ECDLHNFIMNDLKRRTQIVYPKDASYIIFKLDIAPGKRIGEAGTGSGALTSVFSRMVGRD
EECHHHHHHHHHHHHHEEECCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCC
GRVYTYEKRKDFIKVIKRNLAQVQEFDNVVLHNQSLEDGIEEKGLDAFFLDVREPWQVLK
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHCCCCEEEECCHHHHHHHH
KVKLSLKGNGHLGLILPTTNQVSKTLEVLEKLNFYVSEVVEVLYREYKLIPERLRPDDTM
HHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VGHTGYLIFARNLID
ECCCEEEEEECCCCC
>Mature Secondary Structure 
AGGLTNKRVITDRKRFKKVISIEPPHKIGLPTGQIDSKVLKDIRPGSYFEINGSRYYVL
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEECCCEEEEE
ECDLHNFIMNDLKRRTQIVYPKDASYIIFKLDIAPGKRIGEAGTGSGALTSVFSRMVGRD
EECHHHHHHHHHHHHHEEECCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCC
GRVYTYEKRKDFIKVIKRNLAQVQEFDNVVLHNQSLEDGIEEKGLDAFFLDVREPWQVLK
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHCCCCEEEECCHHHHHHHH
KVKLSLKGNGHLGLILPTTNQVSKTLEVLEKLNFYVSEVVEVLYREYKLIPERLRPDDTM
HHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VGHTGYLIFARNLID
ECCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA