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The map label for this gene is cycB [H]

Identifier: 220932455

GI number: 220932455

Start: 1736004

End: 1737209

Strand: Reverse

Name: cycB [H]

Synonym: Hore_16190

Alternate gene names: 220932455

Gene position: 1737209-1736004 (Counterclockwise)

Preceding gene: 220932456

Following gene: 220932454

Centisome position: 67.38

GC content: 41.71

Gene sequence:

>1206_bases
ATGAAAAAAGTTTTAACTTTACTCTCTGTTTTAGTTTTAGTTGTTGGTATGACTTTAAGTGCCAGTGCTGTAAAGCTGGT
AGTCTGGGAATCTCCCGGTCCTGAAGAAGAATTTATTCAGGAAATGGGTAAAATTTACACTGAACAAACCGGTGTTGAAA
TTGAGGTGCAACCAGTAGACCAGATTAACCAGGATGATAAACTGGCCCTGGATGGTCCTGCTGGAAAGGGAGCCGATATT
GTTGTCTGGCCCCATGATGGTATCGGTCGTTCTGTAGAGCAGGGTTTAATCTGGCCTATTCCTGAAGATAAGGTGGATAC
CAGTGCCTTTACAGAGTCTTCTCTCAACGCGCTGACTTACAAGGGTAAGCTATATGGATTACCATATGCTGTTGAAAGTG
TGGCCCTGTTATATAACAAGGATCTGTTACCAGAAGTACCTGAAACCTTTGATGAGTTTTTGGCTAAAGTAAAAGAACTA
AATAAACCGGCTGAGGGCCAGTTTGGTTTTATGGCCAACATCGGTGACCTCTACCACGTTTTCGGGTTTATCTCCGGTTA
TGGTGGTTATATCTTTAAACAGACCGAAAATGGTCTTGACATAAATGATATCGGTCTGGATAGCCCTGGTGCTATTAAAG
CCATGAAGTTTATAAAGAGCTTCAGGACCTCAGGTTTAATGCCTGAAGGTACTACCGGTGATGTTATGAATGGTCTCTTT
TCCCAGGGTTCTCTGGCAGCTGTTATTGACGGACTATGGGCTTTAGAAGGTTATCGTGAAGCCGGGGTTAACTTTGGTGT
TGCTCCCCTGCCCAGGCTTGATAATGGTGAATATCCCCATACCTTCATAGGCGTTAAAGGTTACTACATCAGTGCCTTCA
GTGAACATAAAGAAGAAGCCCTGAAATTCATTCAGTGGTTAACCACTAAAGAGAATTCCTTTAAACATTATCAGAAGACA
TATGTAATTCCTCCACGTAAAGATGTAATGGAAATGCCTGAATTTAAAGAAAATAAAGTTGTTGAAGCTTTTGCTATTCA
GGCTTCAAGGGGTATGCCAATGCCGAATGTACCTGAAATGATGGCTGTATGGGAACCGGCTAATAATGCCCTTTCTTTCA
TCCTTCAGGATCAGGTTACACCTGAAGAAGCAGCTAAACTCTGTGTTCAGAGAATCCAGGATAATATTGAAATGATGAAA
GAATAA

Upstream 100 bases:

>100_bases
GAATTTTATTAACTCAAACGTTTGTAAGTAATTTATTCCTCTGGTAATTTCAATATTTAAAATACATTTAAAAAAACAAA
TGAAAGGAAGGGTTTGAATT

Downstream 100 bases:

>100_bases
TCGGAGTTTAAATCTATTCAGATATGAAACAATAAATATTTTCACCGGTTGGCCCTGTCCGCAACGGATGGGGCCAACCC
TTAATAAATACCCAGAGTGA

Product: family 1 extracellular solute-binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MKKVLTLLSVLVLVVGMTLSASAVKLVVWESPGPEEEFIQEMGKIYTEQTGVEIEVQPVDQINQDDKLALDGPAGKGADI
VVWPHDGIGRSVEQGLIWPIPEDKVDTSAFTESSLNALTYKGKLYGLPYAVESVALLYNKDLLPEVPETFDEFLAKVKEL
NKPAEGQFGFMANIGDLYHVFGFISGYGGYIFKQTENGLDINDIGLDSPGAIKAMKFIKSFRTSGLMPEGTTGDVMNGLF
SQGSLAAVIDGLWALEGYREAGVNFGVAPLPRLDNGEYPHTFIGVKGYYISAFSEHKEEALKFIQWLTTKENSFKHYQKT
YVIPPRKDVMEMPEFKENKVVEAFAIQASRGMPMPNVPEMMAVWEPANNALSFILQDQVTPEEAAKLCVQRIQDNIEMMK
E

Sequences:

>Translated_401_residues
MKKVLTLLSVLVLVVGMTLSASAVKLVVWESPGPEEEFIQEMGKIYTEQTGVEIEVQPVDQINQDDKLALDGPAGKGADI
VVWPHDGIGRSVEQGLIWPIPEDKVDTSAFTESSLNALTYKGKLYGLPYAVESVALLYNKDLLPEVPETFDEFLAKVKEL
NKPAEGQFGFMANIGDLYHVFGFISGYGGYIFKQTENGLDINDIGLDSPGAIKAMKFIKSFRTSGLMPEGTTGDVMNGLF
SQGSLAAVIDGLWALEGYREAGVNFGVAPLPRLDNGEYPHTFIGVKGYYISAFSEHKEEALKFIQWLTTKENSFKHYQKT
YVIPPRKDVMEMPEFKENKVVEAFAIQASRGMPMPNVPEMMAVWEPANNALSFILQDQVTPEEAAKLCVQRIQDNIEMMK
E
>Mature_401_residues
MKKVLTLLSVLVLVVGMTLSASAVKLVVWESPGPEEEFIQEMGKIYTEQTGVEIEVQPVDQINQDDKLALDGPAGKGADI
VVWPHDGIGRSVEQGLIWPIPEDKVDTSAFTESSLNALTYKGKLYGLPYAVESVALLYNKDLLPEVPETFDEFLAKVKEL
NKPAEGQFGFMANIGDLYHVFGFISGYGGYIFKQTENGLDINDIGLDSPGAIKAMKFIKSFRTSGLMPEGTTGDVMNGLF
SQGSLAAVIDGLWALEGYREAGVNFGVAPLPRLDNGEYPHTFIGVKGYYISAFSEHKEEALKFIQWLTTKENSFKHYQKT
YVIPPRKDVMEMPEFKENKVVEAFAIQASRGMPMPNVPEMMAVWEPANNALSFILQDQVTPEEAAKLCVQRIQDNIEMMK
E

Specific function: Binding protein for cyclodextrin; involved in its cellular uptake. Interacts with all natural cyclodextrins:alpha, beta and gamma [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=412, Percent_Identity=30.8252427184466, Blast_Score=170, Evalue=1e-43,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 44425; Mature: 44425

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLTLLSVLVLVVGMTLSASAVKLVVWESPGPEEEFIQEMGKIYTEQTGVEIEVQPVD
CHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECHH
QINQDDKLALDGPAGKGADIVVWPHDGIGRSVEQGLIWPIPEDKVDTSAFTESSLNALTY
CCCCCCCEEEECCCCCCCEEEEECCCCCCCHHHCCCCCCCCCCCCCHHHHHHHCCCEEEE
KGKLYGLPYAVESVALLYNKDLLPEVPETFDEFLAKVKELNKPAEGQFGFMANIGDLYHV
CCEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
FGFISGYGGYIFKQTENGLDINDIGLDSPGAIKAMKFIKSFRTSGLMPEGTTGDVMNGLF
HHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
SQGSLAAVIDGLWALEGYREAGVNFGVAPLPRLDNGEYPHTFIGVKGYYISAFSEHKEEA
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCEEEHHHHHHHHHH
LKFIQWLTTKENSFKHYQKTYVIPPRKDVMEMPEFKENKVVEAFAIQASRGMPMPNVPEM
HHHHHHHHCCCCCHHHHHHEECCCCHHHHHHCCCCCCCCHHHHHEEEHHCCCCCCCCCHH
MAVWEPANNALSFILQDQVTPEEAAKLCVQRIQDNIEMMKE
HEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKVLTLLSVLVLVVGMTLSASAVKLVVWESPGPEEEFIQEMGKIYTEQTGVEIEVQPVD
CHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECHH
QINQDDKLALDGPAGKGADIVVWPHDGIGRSVEQGLIWPIPEDKVDTSAFTESSLNALTY
CCCCCCCEEEECCCCCCCEEEEECCCCCCCHHHCCCCCCCCCCCCCHHHHHHHCCCEEEE
KGKLYGLPYAVESVALLYNKDLLPEVPETFDEFLAKVKELNKPAEGQFGFMANIGDLYHV
CCEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
FGFISGYGGYIFKQTENGLDINDIGLDSPGAIKAMKFIKSFRTSGLMPEGTTGDVMNGLF
HHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
SQGSLAAVIDGLWALEGYREAGVNFGVAPLPRLDNGEYPHTFIGVKGYYISAFSEHKEEA
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCEEEHHHHHHHHHH
LKFIQWLTTKENSFKHYQKTYVIPPRKDVMEMPEFKENKVVEAFAIQASRGMPMPNVPEM
HHHHHHHHCCCCCHHHHHHEECCCCHHHHHHCCCCCCCCHHHHHEEEHHCCCCCCCCCHH
MAVWEPANNALSFILQDQVTPEEAAKLCVQRIQDNIEMMKE
HEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]