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The map label for this gene is rph [H]

Identifier: 220932375

GI number: 220932375

Start: 1647368

End: 1648108

Strand: Reverse

Name: rph [H]

Synonym: Hore_15390

Alternate gene names: 220932375

Gene position: 1648108-1647368 (Counterclockwise)

Preceding gene: 220932376

Following gene: 220932374

Centisome position: 63.93

GC content: 47.91

Gene sequence:

>741_bases
ATGAGTGAAACCAATAATCTCCGGGTAGATGGTCGTAAAAATGACCAGCTACGTGATGTCAAAATAAGCAGGCAATATAC
CAAGTATGCAGAAGGTTCAGTATTGATTGAAACCGGTGATACCAAGGTTATCTGCACAGCCTCAGTGGAGGAAAGTGTTC
CTCCCTTTTTAAGGGGACAGAATACTGGCTGGCTAACGGCTGAATATTCCCTGTTACCGCGGTCTACCCATAGCAGAACC
ATCAGGGAAGTGGCCAGGGGCCGGTTGAGCGGGCGGACCCAGGAGATTCAGCGGTTGATAGGCCGGAGTCTCAGGGCCGT
TGTTGACCTTGGTAAGCTGGGTGAGAGAACCATCTGGGTAGATTGTGATGTTATCCAGGCCGATGGTGGAACCAGAACCG
CTTCTATTACCGGGGCCTATGTGGCTTTAGTTGACGCCATTAATTATCTGATTGAAGAAGGAGTTTTAGAAGAATCACCG
TTAAATAGTTTTATGGCCGCCACCAGTGTTGGTGTAGTCGATGGTGAGATGTTGCTCGATCTGTGTTATGAAGAAGACTT
TCAGGCCCAGGTCGATATGAATATAGTTATGACCGAAGATGGTCGGGTTATTGAAATTCAGGGTACTGCCGAGAAATTCC
CCTTCTCCCGGGACCAGATGAATACTTTGATGGACCTGGCTGAAAAGGGAATCAGGGAACTGGTTTCCTTCCAGAAAAAG
AGCCTGGGGATTGAGGAATAA

Upstream 100 bases:

>100_bases
ACAAAGCTATAATTTGTAACCATATCCCGAATTATAGTTGTAAAATATATTATAAGGATAATAATAAGAAAAAACAAAAA
AAGGGAAGGTGTCTTATTGT

Downstream 100 bases:

>100_bases
TGCCCCTTAAACTCCTTGTAGCCAGTGGTAACCAGGGGAAGATAAGGGAGATAAAAAAATACCTTAATGACCTTGATATA
GAGATTGTTGGCCTTGACGA

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSETNNLRVDGRKNDQLRDVKISRQYTKYAEGSVLIETGDTKVICTASVEESVPPFLRGQNTGWLTAEYSLLPRSTHSRT
IREVARGRLSGRTQEIQRLIGRSLRAVVDLGKLGERTIWVDCDVIQADGGTRTASITGAYVALVDAINYLIEEGVLEESP
LNSFMAATSVGVVDGEMLLDLCYEEDFQAQVDMNIVMTEDGRVIEIQGTAEKFPFSRDQMNTLMDLAEKGIRELVSFQKK
SLGIEE

Sequences:

>Translated_246_residues
MSETNNLRVDGRKNDQLRDVKISRQYTKYAEGSVLIETGDTKVICTASVEESVPPFLRGQNTGWLTAEYSLLPRSTHSRT
IREVARGRLSGRTQEIQRLIGRSLRAVVDLGKLGERTIWVDCDVIQADGGTRTASITGAYVALVDAINYLIEEGVLEESP
LNSFMAATSVGVVDGEMLLDLCYEEDFQAQVDMNIVMTEDGRVIEIQGTAEKFPFSRDQMNTLMDLAEKGIRELVSFQKK
SLGIEE
>Mature_245_residues
SETNNLRVDGRKNDQLRDVKISRQYTKYAEGSVLIETGDTKVICTASVEESVPPFLRGQNTGWLTAEYSLLPRSTHSRTI
REVARGRLSGRTQEIQRLIGRSLRAVVDLGKLGERTIWVDCDVIQADGGTRTASITGAYVALVDAINYLIEEGVLEESPL
NSFMAATSVGVVDGEMLLDLCYEEDFQAQVDMNIVMTEDGRVIEIQGTAEKFPFSRDQMNTLMDLAEKGIRELVSFQKKS
LGIEE

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=63.9810426540284, Blast_Score=286, Evalue=6e-79,
Organism=Caenorhabditis elegans, GI71981632, Length=227, Percent_Identity=25.9911894273128, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 27360; Mature: 27229

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETNNLRVDGRKNDQLRDVKISRQYTKYAEGSVLIETGDTKVICTASVEESVPPFLRGQ
CCCCCCEEECCCCCCCCCEEEHHHHHHHHCCCEEEEEECCCEEEEEECCHHCCCHHHCCC
NTGWLTAEYSLLPRSTHSRTIREVARGRLSGRTQEIQRLIGRSLRAVVDLGKLGERTIWV
CCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEE
DCDVIQADGGTRTASITGAYVALVDAINYLIEEGVLEESPLNSFMAATSVGVVDGEMLLD
EEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHH
LCYEEDFQAQVDMNIVMTEDGRVIEIQGTAEKFPFSRDQMNTLMDLAEKGIRELVSFQKK
HHHCCCCCEEEEEEEEEECCCCEEEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLGIEE
HCCCCC
>Mature Secondary Structure 
SETNNLRVDGRKNDQLRDVKISRQYTKYAEGSVLIETGDTKVICTASVEESVPPFLRGQ
CCCCCEEECCCCCCCCCEEEHHHHHHHHCCCEEEEEECCCEEEEEECCHHCCCHHHCCC
NTGWLTAEYSLLPRSTHSRTIREVARGRLSGRTQEIQRLIGRSLRAVVDLGKLGERTIWV
CCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEE
DCDVIQADGGTRTASITGAYVALVDAINYLIEEGVLEESPLNSFMAATSVGVVDGEMLLD
EEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHH
LCYEEDFQAQVDMNIVMTEDGRVIEIQGTAEKFPFSRDQMNTLMDLAEKGIRELVSFQKK
HHHCCCCCEEEEEEEEEECCCCEEEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLGIEE
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA