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The map label for this gene is cca [H]

Identifier: 220932275

GI number: 220932275

Start: 1524403

End: 1525839

Strand: Reverse

Name: cca [H]

Synonym: Hore_14390

Alternate gene names: 220932275

Gene position: 1525839-1524403 (Counterclockwise)

Preceding gene: 220932276

Following gene: 220932273

Centisome position: 59.18

GC content: 38.83

Gene sequence:

>1437_bases
GTGTTTACAAAAGATTATAAACTGATTTTCCCTGTCTTACAGATACTCCCTGTCTTAAGGGATATCCATGACAGATATCA
GCAGGAATTATATCTCGTCGGGGGTGCTGTCAGGGATATCCTGCTTCAGAGAAATCTAAAGGACTTTGATTTTGTCTTAA
AAGAACGGGTTAGAGAAGTAGCATTGGATTTCAGCTCTAAAACAGGGGGTTCTTTTGTACCTCTGGATAGAGAAAGGGAT
ATTTACCGGGTGGTTGCTGATGGGTATATTTATGATTTTGCTAAAATGGTGGGGTCTACCCTGAAAGAAGATCTATCCAG
ACGTGATTTTACCATTAATTCTATGGCGGTAAATGTAATGGATATTCCCGGTAATTTCGGTAATAAAGAGCCATATCTAA
TTACCGAATATATAAATGATTACATAATAGACCCCTTTGGTGGCTGGACTGATCTGGAGAAGGGTATTATTAGGGTTACT
GATGACAAAGTTTTTATCAAGGACCCACTTCGTCTCTTAAGGGCTTTCAGGTTTAAATCAGAATTAGGATTTTTAATTGA
AAAAGATACTGAGAACCTGATTCACAGGGCCTCTCCCCTTATCAGGAAAATTGCCAGGGAACGGATTGGTTATGAATTTA
GCAGGATATTATCAAACCCCGGGGTGGCAAGAGTAGTTAAATATGCAGAAGATAATTTTAATCTCTTTTCTTATGTTATT
CCTGTTATTAAAAGAATGAAAGAAAAAACACTTGAAGAGGGGTCAGGTAAGGGGTGGAATCTGGATGCCTGGAATCTGGG
AATCAGGACACTTGAATGCCTTGAAGATTACATTAGTAAGGGTTTTTATAGAGATAGAATTAAAGATGACAGGCTGGCCT
GCCTTAAATTAGCCGCCTTCTTTTTCTATATTGGTAACATTAAGGGTAGTCGAATGAAAAGCAGGGCTAAATACACCCCT
GATAGGCAGGGGGGCAGGATGGTTGAAAAAATATTACTGGATTTAAGGATGGGCAAAAAGTGCCGGAGGTATGTGAAACA
GCTTATCTGTTATCATGACTATCCGTATTATTTATTTCAAAAGAAGAAATTACAACCCCTGGATAAAACCCGTTTCTGGC
TGGAATTAAGCGGAGACGGGTTTGATGTTTGTCTCCTGTCTCTGGCCAGTTTTACTGGCAGCAATTCAGAGCCGGTTAGA
ATAAAAGCCTACAGGGAGTTTATTAATGGTTTGCTATTATATGGAGACCGGGTCAAAAAGCTAACAACTACCCCTTTGCT
AAATGGGGAGGAAATAAGGAGTTGTTTTAACCTTGAGGAAGGGCCTGTCATTGGATATTTATTAAAAAAGCTTAAAGAAG
CCCAGGCAAACGGTAAAGTTATGACCAGAAAAGAGGCTATAAATTATCTGGAGAAATATATAAAAGAGGAATTATAG

Upstream 100 bases:

>100_bases
ATACCCAGATCAAGAATGGAGACAGTATTGAAATAGCTTTAAATGATTCTGACAGGAAACAGAAAAGTAATGAAACTGGA
CCAGGGGGTTTGTATTAAAG

Downstream 100 bases:

>100_bases
GTTTGTGATATTATGTTTTTGTTAATATATTATTGACAATATAGTGTTAAGTAAAGTTAGGTATATTAGGATTAAAGATA
ATAATAATTTATGAGGGCAA

Product: poly(A) polymerase/tRNA nucleotidyl transferase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MFTKDYKLIFPVLQILPVLRDIHDRYQQELYLVGGAVRDILLQRNLKDFDFVLKERVREVALDFSSKTGGSFVPLDRERD
IYRVVADGYIYDFAKMVGSTLKEDLSRRDFTINSMAVNVMDIPGNFGNKEPYLITEYINDYIIDPFGGWTDLEKGIIRVT
DDKVFIKDPLRLLRAFRFKSELGFLIEKDTENLIHRASPLIRKIARERIGYEFSRILSNPGVARVVKYAEDNFNLFSYVI
PVIKRMKEKTLEEGSGKGWNLDAWNLGIRTLECLEDYISKGFYRDRIKDDRLACLKLAAFFFYIGNIKGSRMKSRAKYTP
DRQGGRMVEKILLDLRMGKKCRRYVKQLICYHDYPYYLFQKKKLQPLDKTRFWLELSGDGFDVCLLSLASFTGSNSEPVR
IKAYREFINGLLLYGDRVKKLTTTPLLNGEEIRSCFNLEEGPVIGYLLKKLKEAQANGKVMTRKEAINYLEKYIKEEL

Sequences:

>Translated_478_residues
MFTKDYKLIFPVLQILPVLRDIHDRYQQELYLVGGAVRDILLQRNLKDFDFVLKERVREVALDFSSKTGGSFVPLDRERD
IYRVVADGYIYDFAKMVGSTLKEDLSRRDFTINSMAVNVMDIPGNFGNKEPYLITEYINDYIIDPFGGWTDLEKGIIRVT
DDKVFIKDPLRLLRAFRFKSELGFLIEKDTENLIHRASPLIRKIARERIGYEFSRILSNPGVARVVKYAEDNFNLFSYVI
PVIKRMKEKTLEEGSGKGWNLDAWNLGIRTLECLEDYISKGFYRDRIKDDRLACLKLAAFFFYIGNIKGSRMKSRAKYTP
DRQGGRMVEKILLDLRMGKKCRRYVKQLICYHDYPYYLFQKKKLQPLDKTRFWLELSGDGFDVCLLSLASFTGSNSEPVR
IKAYREFINGLLLYGDRVKKLTTTPLLNGEEIRSCFNLEEGPVIGYLLKKLKEAQANGKVMTRKEAINYLEKYIKEEL
>Mature_478_residues
MFTKDYKLIFPVLQILPVLRDIHDRYQQELYLVGGAVRDILLQRNLKDFDFVLKERVREVALDFSSKTGGSFVPLDRERD
IYRVVADGYIYDFAKMVGSTLKEDLSRRDFTINSMAVNVMDIPGNFGNKEPYLITEYINDYIIDPFGGWTDLEKGIIRVT
DDKVFIKDPLRLLRAFRFKSELGFLIEKDTENLIHRASPLIRKIARERIGYEFSRILSNPGVARVVKYAEDNFNLFSYVI
PVIKRMKEKTLEEGSGKGWNLDAWNLGIRTLECLEDYISKGFYRDRIKDDRLACLKLAAFFFYIGNIKGSRMKSRAKYTP
DRQGGRMVEKILLDLRMGKKCRRYVKQLICYHDYPYYLFQKKKLQPLDKTRFWLELSGDGFDVCLLSLASFTGSNSEPVR
IKAYREFINGLLLYGDRVKKLTTTPLLNGEEIRSCFNLEEGPVIGYLLKKLKEAQANGKVMTRKEAINYLEKYIKEEL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081691, Length=240, Percent_Identity=29.1666666666667, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789436, Length=211, Percent_Identity=30.3317535545024, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 55766; Mature: 55766

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00014 ER_TARGET

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTKDYKLIFPVLQILPVLRDIHDRYQQELYLVGGAVRDILLQRNLKDFDFVLKERVREV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ALDFSSKTGGSFVPLDRERDIYRVVADGYIYDFAKMVGSTLKEDLSRRDFTINSMAVNVM
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
DIPGNFGNKEPYLITEYINDYIIDPFGGWTDLEKGIIRVTDDKVFIKDPLRLLRAFRFKS
ECCCCCCCCCCEEEHHHHHHHEECCCCCHHHHCCCEEEEECCCEEHHHHHHHHHHHHHHH
ELGFLIEKDTENLIHRASPLIRKIARERIGYEFSRILSNPGVARVVKYAEDNFNLFSYVI
HHCCEEECCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHCCCCHHHHHHH
PVIKRMKEKTLEEGSGKGWNLDAWNLGIRTLECLEDYISKGFYRDRIKDDRLACLKLAAF
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHH
FFYIGNIKGSRMKSRAKYTPDRQGGRMVEKILLDLRMGKKCRRYVKQLICYHDYPYYLFQ
HHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHC
KKKLQPLDKTRFWLELSGDGFDVCLLSLASFTGSNSEPVRIKAYREFINGLLLYGDRVKK
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCHHHH
LTTTPLLNGEEIRSCFNLEEGPVIGYLLKKLKEAQANGKVMTRKEAINYLEKYIKEEL
HHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFTKDYKLIFPVLQILPVLRDIHDRYQQELYLVGGAVRDILLQRNLKDFDFVLKERVREV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ALDFSSKTGGSFVPLDRERDIYRVVADGYIYDFAKMVGSTLKEDLSRRDFTINSMAVNVM
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
DIPGNFGNKEPYLITEYINDYIIDPFGGWTDLEKGIIRVTDDKVFIKDPLRLLRAFRFKS
ECCCCCCCCCCEEEHHHHHHHEECCCCCHHHHCCCEEEEECCCEEHHHHHHHHHHHHHHH
ELGFLIEKDTENLIHRASPLIRKIARERIGYEFSRILSNPGVARVVKYAEDNFNLFSYVI
HHCCEEECCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHCCCCHHHHHHH
PVIKRMKEKTLEEGSGKGWNLDAWNLGIRTLECLEDYISKGFYRDRIKDDRLACLKLAAF
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHH
FFYIGNIKGSRMKSRAKYTPDRQGGRMVEKILLDLRMGKKCRRYVKQLICYHDYPYYLFQ
HHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHC
KKKLQPLDKTRFWLELSGDGFDVCLLSLASFTGSNSEPVRIKAYREFINGLLLYGDRVKK
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCHHHH
LTTTPLLNGEEIRSCFNLEEGPVIGYLLKKLKEAQANGKVMTRKEAINYLEKYIKEEL
HHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA