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The map label for this gene is folC [H]

Identifier: 220932273

GI number: 220932273

Start: 1522357

End: 1523730

Strand: Reverse

Name: folC [H]

Synonym: Hore_14370

Alternate gene names: 220932273

Gene position: 1523730-1522357 (Counterclockwise)

Preceding gene: 220932275

Following gene: 220932272

Centisome position: 59.1

GC content: 41.48

Gene sequence:

>1374_bases
ATGGACCCCTATAAGTATATTAATAGTTTATCTAGATTCGGGACAGATGCTGGATTTAAGCCAGGGCTTGACAGGATAAA
TGCCTTACTGGGAAGCTTTAACCACCCGGAAGACCGGTTAAAGGTTATTCACGTAGCCGGAACCAATGGTAAGGGTTCAA
CGATAGCTTTTTTAAAAAGTATATATACTGAAGCCGGGTACCGGGTCGGAGTCTATACTTCACCACATTTGATTCACTTT
AATGAAAGAATAGAAGTCGGGAACAGGGCTATTAGTACAGCTGAGCTAACAGACCTTGTTTCTGAACTGGTTCCCCGGGT
TGAAGATTTGAAGAAAGACCCGGAGGTTGGCGGTCCGACCTATTTTGAGTTTGTGACAGCCCTGGCTTTATTATATTTTG
CCCGAAAAGATGTAGACCTGGTTCTGCTTGAAGTTGGGCTGGGAGGCAGACTGGATGCCACCAATGTTATTAAAGAGCCC
CTGGTAAGTGTAATTACAAGTATATCCCTTGATCACACCTCCATTCTGGGAAATGATGTAAAAAGTATTGCCCGGGAAAA
GTCAGGTATTATTAAAAGGGACTGTCCTGTAGTTACCGGGGTCAGGAATAAGGAGGCCTTAAGAGTAATTGAAGAGACTG
CTAAATTAAAAAATTCCCCCCTGAAAATTATTGATAAAGAGTATTCATACAACATAATTGAGAGTTCTCTGGAGGGCCAG
TCATTTACCGTATTCCGTCTTAATAATGGGGATTACAGCCTGGAGAAAGAAGCAGAATCTATAAACGGGACTGAGAAGAT
TGAGTATTTTATTAAACTTCCCGGTGTTTATCAGGTCCGAAATGCCCTGCTGGCGATGGCAGTGGTTGATTACTTACAGG
AACAGTATCCTGTAAATCTAGTACAAATTAAAAAGGGTCTAGCCTCAGCTTTTATTCCGGGGCGGATGGAGGTTTTAGCC
CGGTCTCCGCTGATTATAGCCGATGGTGCCCATAATGTTGAAGGGGTAAAAAAACTGGTAAATAGTTTGGGACCATTAAT
TAATGAAGATAGACAATTTTATATAATAGTCGGCATCCTGGGGGATAAGGATGTTGAAGGAATGATAGAGATGCTTGATT
TTGGAAAGGCTAAAAAAGAGCTGATAATTACCAGAAATACTAACAGCCGGTCCTGTTCCCCTGAAGTTATCGGTCAATAT
GCTCAAAGACGGGGAATAAAGTACAGGGTATATCCTGATTTAAAGACCTCCCTCACGGGGGTGTTAAATGATGCCAGGCC
TGAAGATGTAATATGTGTCACCGGTTCTCTATATACAGTTTCAGAAGCTAAAATATTAGTTCAAAGTGGTATAAAACATA
ACAGTCAATCATAA

Upstream 100 bases:

>100_bases
TTTGTATATAAAAGGTGTTAATTACGGGTGCAGTTAACCCTGTGAACTGGATTCAAAATGTATACAGACAGGATGGGAGT
TGCGGAAAGGAAGATGAACC

Downstream 100 bases:

>100_bases
TAGTATAAAGAAAAGGAGTGTGGTGAAAAAATGAGCAGGCAGAATACTTTTTCACTTACCATTATGGTAATTGTTATGGC
CCTGCTGGCAATATTTGTTG

Product: Folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MDPYKYINSLSRFGTDAGFKPGLDRINALLGSFNHPEDRLKVIHVAGTNGKGSTIAFLKSIYTEAGYRVGVYTSPHLIHF
NERIEVGNRAISTAELTDLVSELVPRVEDLKKDPEVGGPTYFEFVTALALLYFARKDVDLVLLEVGLGGRLDATNVIKEP
LVSVITSISLDHTSILGNDVKSIAREKSGIIKRDCPVVTGVRNKEALRVIEETAKLKNSPLKIIDKEYSYNIIESSLEGQ
SFTVFRLNNGDYSLEKEAESINGTEKIEYFIKLPGVYQVRNALLAMAVVDYLQEQYPVNLVQIKKGLASAFIPGRMEVLA
RSPLIIADGAHNVEGVKKLVNSLGPLINEDRQFYIIVGILGDKDVEGMIEMLDFGKAKKELIITRNTNSRSCSPEVIGQY
AQRRGIKYRVYPDLKTSLTGVLNDARPEDVICVTGSLYTVSEAKILVQSGIKHNSQS

Sequences:

>Translated_457_residues
MDPYKYINSLSRFGTDAGFKPGLDRINALLGSFNHPEDRLKVIHVAGTNGKGSTIAFLKSIYTEAGYRVGVYTSPHLIHF
NERIEVGNRAISTAELTDLVSELVPRVEDLKKDPEVGGPTYFEFVTALALLYFARKDVDLVLLEVGLGGRLDATNVIKEP
LVSVITSISLDHTSILGNDVKSIAREKSGIIKRDCPVVTGVRNKEALRVIEETAKLKNSPLKIIDKEYSYNIIESSLEGQ
SFTVFRLNNGDYSLEKEAESINGTEKIEYFIKLPGVYQVRNALLAMAVVDYLQEQYPVNLVQIKKGLASAFIPGRMEVLA
RSPLIIADGAHNVEGVKKLVNSLGPLINEDRQFYIIVGILGDKDVEGMIEMLDFGKAKKELIITRNTNSRSCSPEVIGQY
AQRRGIKYRVYPDLKTSLTGVLNDARPEDVICVTGSLYTVSEAKILVQSGIKHNSQS
>Mature_457_residues
MDPYKYINSLSRFGTDAGFKPGLDRINALLGSFNHPEDRLKVIHVAGTNGKGSTIAFLKSIYTEAGYRVGVYTSPHLIHF
NERIEVGNRAISTAELTDLVSELVPRVEDLKKDPEVGGPTYFEFVTALALLYFARKDVDLVLLEVGLGGRLDATNVIKEP
LVSVITSISLDHTSILGNDVKSIAREKSGIIKRDCPVVTGVRNKEALRVIEETAKLKNSPLKIIDKEYSYNIIESSLEGQ
SFTVFRLNNGDYSLEKEAESINGTEKIEYFIKLPGVYQVRNALLAMAVVDYLQEQYPVNLVQIKKGLASAFIPGRMEVLA
RSPLIIADGAHNVEGVKKLVNSLGPLINEDRQFYIIVGILGDKDVEGMIEMLDFGKAKKELIITRNTNSRSCSPEVIGQY
AQRRGIKYRVYPDLKTSLTGVLNDARPEDVICVTGSLYTVSEAKILVQSGIKHNSQS

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=356, Percent_Identity=31.1797752808989, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI66932990, Length=356, Percent_Identity=31.1797752808989, Blast_Score=164, Evalue=2e-40,
Organism=Escherichia coli, GI1788654, Length=426, Percent_Identity=32.1596244131455, Blast_Score=180, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI71984923, Length=184, Percent_Identity=39.6739130434783, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17553150, Length=184, Percent_Identity=39.6739130434783, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17553148, Length=184, Percent_Identity=39.6739130434783, Blast_Score=140, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323760, Length=456, Percent_Identity=31.140350877193, Blast_Score=177, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6324815, Length=195, Percent_Identity=36.4102564102564, Blast_Score=126, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6322718, Length=317, Percent_Identity=25.8675078864353, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24641571, Length=309, Percent_Identity=32.3624595469256, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24581568, Length=215, Percent_Identity=29.7674418604651, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 50499; Mature: 50499

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPYKYINSLSRFGTDAGFKPGLDRINALLGSFNHPEDRLKVIHVAGTNGKGSTIAFLKS
CCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHH
IYTEAGYRVGVYTSPHLIHFNERIEVGNRAISTAELTDLVSELVPRVEDLKKDPEVGGPT
HHHHCCCEEEEECCCEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
YFEFVTALALLYFARKDVDLVLLEVGLGGRLDATNVIKEPLVSVITSISLDHTSILGNDV
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
KSIAREKSGIIKRDCPVVTGVRNKEALRVIEETAKLKNSPLKIIDKEYSYNIIESSLEGQ
HHHHHHHCCCEECCCCEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHCCCCC
SFTVFRLNNGDYSLEKEAESINGTEKIEYFIKLPGVYQVRNALLAMAVVDYLQEQYPVNL
EEEEEEECCCCCCHHHHHHHCCCHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHH
VQIKKGLASAFIPGRMEVLARSPLIIADGAHNVEGVKKLVNSLGPLINEDRQFYIIVGIL
HHHHHHHHHHCCCCHHHHHHCCCEEEECCCCCHHHHHHHHHHHCHHHCCCCEEEEEEEEE
GDKDVEGMIEMLDFGKAKKELIITRNTNSRSCSPEVIGQYAQRRGIKYRVYPDLKTSLTG
CCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHH
VLNDARPEDVICVTGSLYTVSEAKILVQSGIKHNSQS
HHCCCCCCCEEEEECCEEEEHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDPYKYINSLSRFGTDAGFKPGLDRINALLGSFNHPEDRLKVIHVAGTNGKGSTIAFLKS
CCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHH
IYTEAGYRVGVYTSPHLIHFNERIEVGNRAISTAELTDLVSELVPRVEDLKKDPEVGGPT
HHHHCCCEEEEECCCEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
YFEFVTALALLYFARKDVDLVLLEVGLGGRLDATNVIKEPLVSVITSISLDHTSILGNDV
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
KSIAREKSGIIKRDCPVVTGVRNKEALRVIEETAKLKNSPLKIIDKEYSYNIIESSLEGQ
HHHHHHHCCCEECCCCEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHCCCCC
SFTVFRLNNGDYSLEKEAESINGTEKIEYFIKLPGVYQVRNALLAMAVVDYLQEQYPVNL
EEEEEEECCCCCCHHHHHHHCCCHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHH
VQIKKGLASAFIPGRMEVLARSPLIIADGAHNVEGVKKLVNSLGPLINEDRQFYIIVGIL
HHHHHHHHHHCCCCHHHHHHCCCEEEECCCCCHHHHHHHHHHHCHHHCCCCEEEEEEEEE
GDKDVEGMIEMLDFGKAKKELIITRNTNSRSCSPEVIGQYAQRRGIKYRVYPDLKTSLTG
CCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHH
VLNDARPEDVICVTGSLYTVSEAKILVQSGIKHNSQS
HHCCCCCCCEEEEECCEEEEHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]