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The map label for this gene is nfsA [C]

Identifier: 220932159

GI number: 220932159

Start: 1421543

End: 1422379

Strand: Reverse

Name: nfsA [C]

Synonym: Hore_13220

Alternate gene names: 220932159

Gene position: 1422379-1421543 (Counterclockwise)

Preceding gene: 220932160

Following gene: 220932158

Centisome position: 55.17

GC content: 39.67

Gene sequence:

>837_bases
ATGTCCGGTATTGTATTTTTTAAGACAACCAGGCTGGAAGAAATAAAAAAATTTTACCAGGATAGAATAGGTATGGACCT
CTGGTTAGATCAGGGGCAATGTCTTATTTTTGAAAAGGGTAATTTACAGCTTGGTTTCTGTAAGGGAGATAGAGCTGACA
TAGAAGGGATTATTACTTTTTACTTTGATTCAAAATCAACTGTGGATGAATATTATCAACGGTTTGAGAAGGAAGCCCTT
GATAAACCGGTTATAAATGAAAAATTTAATATTTATCATTTTTATATAAAAGACCCGGAAGGGCGAAAGGTAGAATTTCA
GACTTTTCTCCATGATCTTAATCCTTATCTTACCGGTGTTGAATTACTCCTGACACGTCGGAGTATTCGAAAATACAGGG
ATAGAGAAGTCCCGGAAGAAATACTGTGGAACGTTTTTGAGAGGTGCCGTTATGCCCCAACTTCCCGCAATTCCCAGTCC
TATTATTTTGTTGTGGTCAAAGATAGAAACAAATTAGAATACCTGGCCTCTTTAAGAGGTAGTAACAGCAGACCTATTGC
AACTGCTCCTATGGCTGTAGCTATTTGCTCTGATAGTAAAAAATCTAAAAGGTTTATTCAGGATGGCTGTATTGCCGCCT
ACCATTTTCTTTTAGCAGCCCGTCTGTTCGGTCTGGGTACCTGCTGGATAGCAGCCATGGATAGAAAAGAAGTCAAAAAG
GTCCTTGATATTCCCCGGGATCATTATGTAGCCACGGTAACCCCACTTGGTTATCCTGCCGAAAAACCAAATATACCTCC
CAGGAAGAAGGTTCAGGAGATGGTCAGGTTTGTGTAA

Upstream 100 bases:

>100_bases
CTGGGGATTTTACTTATAGTTGGCTGGGTGGTTTTCCGTTATAAAAAAGCAGGTATTTGTACCGGGTTGGCAGAGTATAA
CCATATTAAGGAGGTGTAAA

Downstream 100 bases:

>100_bases
CTGGTAAAAGACCGGGCTGTTATTTGTGGTTGTTTTTATAACCAGAGAAAAGCCCGGTTTAATTTTTACTTTTTTCAGTG
GTGTTACTTACATAAAAGTA

Product: nitroreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MSGIVFFKTTRLEEIKKFYQDRIGMDLWLDQGQCLIFEKGNLQLGFCKGDRADIEGIITFYFDSKSTVDEYYQRFEKEAL
DKPVINEKFNIYHFYIKDPEGRKVEFQTFLHDLNPYLTGVELLLTRRSIRKYRDREVPEEILWNVFERCRYAPTSRNSQS
YYFVVVKDRNKLEYLASLRGSNSRPIATAPMAVAICSDSKKSKRFIQDGCIAAYHFLLAARLFGLGTCWIAAMDRKEVKK
VLDIPRDHYVATVTPLGYPAEKPNIPPRKKVQEMVRFV

Sequences:

>Translated_278_residues
MSGIVFFKTTRLEEIKKFYQDRIGMDLWLDQGQCLIFEKGNLQLGFCKGDRADIEGIITFYFDSKSTVDEYYQRFEKEAL
DKPVINEKFNIYHFYIKDPEGRKVEFQTFLHDLNPYLTGVELLLTRRSIRKYRDREVPEEILWNVFERCRYAPTSRNSQS
YYFVVVKDRNKLEYLASLRGSNSRPIATAPMAVAICSDSKKSKRFIQDGCIAAYHFLLAARLFGLGTCWIAAMDRKEVKK
VLDIPRDHYVATVTPLGYPAEKPNIPPRKKVQEMVRFV
>Mature_277_residues
SGIVFFKTTRLEEIKKFYQDRIGMDLWLDQGQCLIFEKGNLQLGFCKGDRADIEGIITFYFDSKSTVDEYYQRFEKEALD
KPVINEKFNIYHFYIKDPEGRKVEFQTFLHDLNPYLTGVELLLTRRSIRKYRDREVPEEILWNVFERCRYAPTSRNSQSY
YFVVVKDRNKLEYLASLRGSNSRPIATAPMAVAICSDSKKSKRFIQDGCIAAYHFLLAARLFGLGTCWIAAMDRKEVKKV
LDIPRDHYVATVTPLGYPAEKPNIPPRKKVQEMVRFV

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 32552; Mature: 32421

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGIVFFKTTRLEEIKKFYQDRIGMDLWLDQGQCLIFEKGNLQLGFCKGDRADIEGIITF
CCCEEEEEHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCEEEEEECCCCCCCCEEEEE
YFDSKSTVDEYYQRFEKEALDKPVINEKFNIYHFYIKDPEGRKVEFQTFLHDLNPYLTGV
EECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCEEHHHHHHHHHCHHHHHH
ELLLTRRSIRKYRDREVPEEILWNVFERCRYAPTSRNSQSYYFVVVKDRNKLEYLASLRG
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHHCC
SNSRPIATAPMAVAICSDSKKSKRFIQDGCIAAYHFLLAARLFGLGTCWIAAMDRKEVKK
CCCCCEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
VLDIPRDHYVATVTPLGYPAEKPNIPPRKKVQEMVRFV
HHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SGIVFFKTTRLEEIKKFYQDRIGMDLWLDQGQCLIFEKGNLQLGFCKGDRADIEGIITF
CCEEEEEHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCEEEEEECCCCCCCCEEEEE
YFDSKSTVDEYYQRFEKEALDKPVINEKFNIYHFYIKDPEGRKVEFQTFLHDLNPYLTGV
EECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCEEHHHHHHHHHCHHHHHH
ELLLTRRSIRKYRDREVPEEILWNVFERCRYAPTSRNSQSYYFVVVKDRNKLEYLASLRG
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHHCC
SNSRPIATAPMAVAICSDSKKSKRFIQDGCIAAYHFLLAARLFGLGTCWIAAMDRKEVKK
CCCCCEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
VLDIPRDHYVATVTPLGYPAEKPNIPPRKKVQEMVRFV
HHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]