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The map label for this gene is rsmE [H]

Identifier: 220932114

GI number: 220932114

Start: 1370054

End: 1370803

Strand: Reverse

Name: rsmE [H]

Synonym: Hore_12770

Alternate gene names: 220932114

Gene position: 1370803-1370054 (Counterclockwise)

Preceding gene: 220932115

Following gene: 220932113

Centisome position: 53.17

GC content: 37.47

Gene sequence:

>750_bases
ATGCACCGTTTTTTTGTATCTGAAGAACAAATACGGGATGATCTGGTAATAATAAAAGGGAATGATTATAACCATATTGT
TAATTCTTTACGGCTGAATCCGGGTGATAAACTCATTGTCTGCCCCGGAAACTGTCGGGATTATGTTGCTACTATTGAGG
AGATAAAGGAAAACCAGATAACCGGGAAAATAATTAAAGAATATAAAAACAGGAATGAACCGTCATTAAATATTACCCTG
GCCCAGGCTCTTCCTAAAAAAAGAAAAATGGATTTAGTTATCCAGAAGGCAACTGAAATCGGGGTTCGTAGTATTATCCC
CCTTAAAACAACAAGGACTATAGTTAGATTAAATAAAAAAAAAGAACGAAAGAAAACCGATAGATGGCAAAAGATTGCTA
AAGAAGCTGCCAAACAGTCGGGGCGGGGAAGGATACCCACAGTTGAAAGGGTATATAGTCTCAAAGAGTTAACGCAATTT
AAATCTAATTTTGATCTTATAATAATTCCCTGGGAAGAGGAACAGGGTAAAAACTTAAAAAACGTTTTATCAACAGTAAA
TGTCAGTGAAATCAGTAAGATATTAGTGATAATCGGTCCTGAAGGGGGCTTTCCCCCGGAGGAAATTAAGTTTATTGAAC
AACTTGGGGGAATTCCTATAACTTTAGGACCACGTATCTTGAGGACTGAAACTGCTGGACTGGTGGTATTGACCATGCTA
TTATATGAATCAGGTGATTTAGGGGGCTGA

Upstream 100 bases:

>100_bases
GAATTGCTACGGTTTTAGTAAATTCCGGGTTTAACCTGCTAGAAGAAGTAAAACTCAACGAATGGGTAAGTCTGGTTGCC
AGAAAGGATTGAGCTGTTTT

Downstream 100 bases:

>100_bases
TGATGAATACAGTAGCATTCCACACCCTTGGTTGTAAGGTGAATCATTATGAGACAGAAGCCATGATGGGCATATTTGAA
GAGGCCGGTTATAAAGTTGT

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MHRFFVSEEQIRDDLVIIKGNDYNHIVNSLRLNPGDKLIVCPGNCRDYVATIEEIKENQITGKIIKEYKNRNEPSLNITL
AQALPKKRKMDLVIQKATEIGVRSIIPLKTTRTIVRLNKKKERKKTDRWQKIAKEAAKQSGRGRIPTVERVYSLKELTQF
KSNFDLIIIPWEEEQGKNLKNVLSTVNVSEISKILVIIGPEGGFPPEEIKFIEQLGGIPITLGPRILRTETAGLVVLTML
LYESGDLGG

Sequences:

>Translated_249_residues
MHRFFVSEEQIRDDLVIIKGNDYNHIVNSLRLNPGDKLIVCPGNCRDYVATIEEIKENQITGKIIKEYKNRNEPSLNITL
AQALPKKRKMDLVIQKATEIGVRSIIPLKTTRTIVRLNKKKERKKTDRWQKIAKEAAKQSGRGRIPTVERVYSLKELTQF
KSNFDLIIIPWEEEQGKNLKNVLSTVNVSEISKILVIIGPEGGFPPEEIKFIEQLGGIPITLGPRILRTETAGLVVLTML
LYESGDLGG
>Mature_249_residues
MHRFFVSEEQIRDDLVIIKGNDYNHIVNSLRLNPGDKLIVCPGNCRDYVATIEEIKENQITGKIIKEYKNRNEPSLNITL
AQALPKKRKMDLVIQKATEIGVRSIIPLKTTRTIVRLNKKKERKKTDRWQKIAKEAAKQSGRGRIPTVERVYSLKELTQF
KSNFDLIIIPWEEEQGKNLKNVLSTVNVSEISKILVIIGPEGGFPPEEIKFIEQLGGIPITLGPRILRTETAGLVVLTML
LYESGDLGG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=254, Percent_Identity=31.1023622047244, Blast_Score=123, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 28226; Mature: 28226

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRFFVSEEQIRDDLVIIKGNDYNHIVNSLRLNPGDKLIVCPGNCRDYVATIEEIKENQI
CCCCCCCHHHHCCCEEEEECCCHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHCCH
TGKIIKEYKNRNEPSLNITLAQALPKKRKMDLVIQKATEIGVRSIIPLKTTRTIVRLNKK
HHHHHHHHHCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHH
KERKKTDRWQKIAKEAAKQSGRGRIPTVERVYSLKELTQFKSNFDLIIIPWEEEQGKNLK
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHH
NVLSTVNVSEISKILVIIGPEGGFPPEEIKFIEQLGGIPITLGPRILRTETAGLVVLTML
HHHHHCCHHHHCEEEEEECCCCCCCHHHHHHHHHHCCCEEEECCEEEEECHHHHHHHHHH
LYESGDLGG
HHCCCCCCC
>Mature Secondary Structure
MHRFFVSEEQIRDDLVIIKGNDYNHIVNSLRLNPGDKLIVCPGNCRDYVATIEEIKENQI
CCCCCCCHHHHCCCEEEEECCCHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHCCH
TGKIIKEYKNRNEPSLNITLAQALPKKRKMDLVIQKATEIGVRSIIPLKTTRTIVRLNKK
HHHHHHHHHCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHH
KERKKTDRWQKIAKEAAKQSGRGRIPTVERVYSLKELTQFKSNFDLIIIPWEEEQGKNLK
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHH
NVLSTVNVSEISKILVIIGPEGGFPPEEIKFIEQLGGIPITLGPRILRTETAGLVVLTML
HHHHHCCHHHHCEEEEEECCCCCCCHHHHHHHHHHCCCEEEECCEEEEECHHHHHHHHHH
LYESGDLGG
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]