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The map label for this gene is tgt

Identifier: 220932066

GI number: 220932066

Start: 1322662

End: 1323783

Strand: Reverse

Name: tgt

Synonym: Hore_12290

Alternate gene names: 220932066

Gene position: 1323783-1322662 (Counterclockwise)

Preceding gene: 220932067

Following gene: 220932065

Centisome position: 51.35

GC content: 42.69

Gene sequence:

>1122_bases
ATGTCCATTAGTTTCAATCTATTACATCGAGAGATAAACACCCGGGCCCGGCTGGGAAAGCTCAAAACACCACATGGTGA
TATAGATACTCCTATTTTTATGCCGGTAGGAACCCAGGCTACAGTTAAATCTATGACTCCTGATGAATTAGAGAATATTA
AGGCCCAGATTATTCTATCAAATACATATCACCTTTACCTGCGGCCAGGTTCGAGTTTGATAGATGAGGCTGGAGGGCTC
CATAACTTTATGAACTGGCAGAAACCAATTTTAACAGATAGTGGGGGCTTTCAGGTATTTAGTCTATCTGACCTCAGAGA
GATAAAAGAAGAAGGAGTTTATTTTAGGTCTCACCTTGATGGTTCCAGGCATTTTATATCCCCTGAAAAGGCTATTCAAA
TTCAGATGGAACTGGGAGCGGATATTATCATGGCTTTTGATGAATGCCCTCCCTATCCCAGTGATTATGATTATGTGGCC
CGGTCCCTTGAAAGAACAGTCAGGTGGGCAAGGCGCTGTAAAAAAGCCCATGAGAGGGAGGACCAGGCCCTGTTTGGAAT
AATTCAGGGTGGTGTCTACCGGGATTTAAGAAAGCAAAGTGTTGAAGCATTGATAGATATTGGTTTTCCGGGGTATGCTA
TAGGTGGATTAAGCGTTGGTGAGGAGAAAGAAAAGATGCTGGAGGTCCTGGACTTTACAGTACCATTGATGCCAGAAAAT
AAACCCCGGTATTTAATGGGTGTTGGTGCTCCTGAAGACCTTGTCGAAGGTGTTATCAGGGGGGTTGATATGTTTGACTG
TGTTTTACCGACCAGAATTGCCAGGCACGGGACTGTCTTTACCAGTCAGGGCCGGCTAACTGTCAGGAATGCCGGGTATG
AGAGAGATTTTACACCCCTTGATCCCGAGTGTGATTGTTATGTCTGTAAAAACTATACCCGGGCCTATATCCGCCACTTA
ATAAAAAGAAAAGAAATCCTTGGTGTAAGATTGACTACCTACCATAACCTATATTTTCTCTTATCTTTGATGGAGAAAAT
AAGGGGAGCTATAAAGGAAGACGCCCTGCTTGAATTTAGAGATGAATTCCTTAGAAAATATTTAAATAAAGCCTCAATTT
AG

Upstream 100 bases:

>100_bases
ATTATGAATCTAAGATCCAGTAAAAGATAGTTTTAAAAGAGGAATTAGAAAAGGAATTACAAATTTGAGATGTACCAGTA
AAAGGGGGATTTATAAGTAT

Downstream 100 bases:

>100_bases
CAGGAATTTAAAGCTATGTGTTGAATAGTAAACAAGAGGTTCTCTTTTTTGGGTAAGGGCCCATTCACTACTATAACCGG
GTAAGAGATTAAAAAAATAA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MSISFNLLHREINTRARLGKLKTPHGDIDTPIFMPVGTQATVKSMTPDELENIKAQIILSNTYHLYLRPGSSLIDEAGGL
HNFMNWQKPILTDSGGFQVFSLSDLREIKEEGVYFRSHLDGSRHFISPEKAIQIQMELGADIIMAFDECPPYPSDYDYVA
RSLERTVRWARRCKKAHEREDQALFGIIQGGVYRDLRKQSVEALIDIGFPGYAIGGLSVGEEKEKMLEVLDFTVPLMPEN
KPRYLMGVGAPEDLVEGVIRGVDMFDCVLPTRIARHGTVFTSQGRLTVRNAGYERDFTPLDPECDCYVCKNYTRAYIRHL
IKRKEILGVRLTTYHNLYFLLSLMEKIRGAIKEDALLEFRDEFLRKYLNKASI

Sequences:

>Translated_373_residues
MSISFNLLHREINTRARLGKLKTPHGDIDTPIFMPVGTQATVKSMTPDELENIKAQIILSNTYHLYLRPGSSLIDEAGGL
HNFMNWQKPILTDSGGFQVFSLSDLREIKEEGVYFRSHLDGSRHFISPEKAIQIQMELGADIIMAFDECPPYPSDYDYVA
RSLERTVRWARRCKKAHEREDQALFGIIQGGVYRDLRKQSVEALIDIGFPGYAIGGLSVGEEKEKMLEVLDFTVPLMPEN
KPRYLMGVGAPEDLVEGVIRGVDMFDCVLPTRIARHGTVFTSQGRLTVRNAGYERDFTPLDPECDCYVCKNYTRAYIRHL
IKRKEILGVRLTTYHNLYFLLSLMEKIRGAIKEDALLEFRDEFLRKYLNKASI
>Mature_372_residues
SISFNLLHREINTRARLGKLKTPHGDIDTPIFMPVGTQATVKSMTPDELENIKAQIILSNTYHLYLRPGSSLIDEAGGLH
NFMNWQKPILTDSGGFQVFSLSDLREIKEEGVYFRSHLDGSRHFISPEKAIQIQMELGADIIMAFDECPPYPSDYDYVAR
SLERTVRWARRCKKAHEREDQALFGIIQGGVYRDLRKQSVEALIDIGFPGYAIGGLSVGEEKEKMLEVLDFTVPLMPENK
PRYLMGVGAPEDLVEGVIRGVDMFDCVLPTRIARHGTVFTSQGRLTVRNAGYERDFTPLDPECDCYVCKNYTRAYIRHLI
KRKEILGVRLTTYHNLYFLLSLMEKIRGAIKEDALLEFRDEFLRKYLNKASI

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=352, Percent_Identity=46.875, Blast_Score=340, Evalue=1e-93,
Organism=Escherichia coli, GI1786607, Length=356, Percent_Identity=56.1797752808989, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17544680, Length=353, Percent_Identity=47.0254957507082, Blast_Score=327, Evalue=5e-90,
Organism=Caenorhabditis elegans, GI25146502, Length=245, Percent_Identity=24.8979591836735, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19920510, Length=361, Percent_Identity=44.3213296398892, Blast_Score=332, Evalue=3e-91,
Organism=Drosophila melanogaster, GI21355311, Length=233, Percent_Identity=27.4678111587983, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_HALOH (B8CXG0)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002508974.1
- ProteinModelPortal:   B8CXG0
- SMR:   B8CXG0
- GeneID:   7313930
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_12290
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 42635; Mature: 42504

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: ACT_SITE 93-93 BINDING 94-94

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISFNLLHREINTRARLGKLKTPHGDIDTPIFMPVGTQATVKSMTPDELENIKAQIILS
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHCCCCHHHHHCCEEEEEEE
NTYHLYLRPGSSLIDEAGGLHNFMNWQKPILTDSGGFQVFSLSDLREIKEEGVYFRSHLD
CCEEEEECCCCHHHHHCCCHHHHHCCCCCCEECCCCEEEEEHHHHHHHHHCCCEEEECCC
GSRHFISPEKAIQIQMELGADIIMAFDECPPYPSDYDYVARSLERTVRWARRCKKAHERE
CCCCCCCCCHHEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DQALFGIIQGGVYRDLRKQSVEALIDIGFPGYAIGGLSVGEEKEKMLEVLDFTVPLMPEN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCC
KPRYLMGVGAPEDLVEGVIRGVDMFDCVLPTRIARHGTVFTSQGRLTVRNAGYERDFTPL
CCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEECCCEEEEECCCCCCCCCCC
DPECDCYVCKNYTRAYIRHLIKRKEILGVRLTTYHNLYFLLSLMEKIRGAIKEDALLEFR
CCCCCEEEHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEFLRKYLNKASI
HHHHHHHHHHCCC
>Mature Secondary Structure 
SISFNLLHREINTRARLGKLKTPHGDIDTPIFMPVGTQATVKSMTPDELENIKAQIILS
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHCCCCHHHHHCCEEEEEEE
NTYHLYLRPGSSLIDEAGGLHNFMNWQKPILTDSGGFQVFSLSDLREIKEEGVYFRSHLD
CCEEEEECCCCHHHHHCCCHHHHHCCCCCCEECCCCEEEEEHHHHHHHHHCCCEEEECCC
GSRHFISPEKAIQIQMELGADIIMAFDECPPYPSDYDYVARSLERTVRWARRCKKAHERE
CCCCCCCCCHHEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DQALFGIIQGGVYRDLRKQSVEALIDIGFPGYAIGGLSVGEEKEKMLEVLDFTVPLMPEN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCC
KPRYLMGVGAPEDLVEGVIRGVDMFDCVLPTRIARHGTVFTSQGRLTVRNAGYERDFTPL
CCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEECCCEEEEECCCCCCCCCCC
DPECDCYVCKNYTRAYIRHLIKRKEILGVRLTTYHNLYFLLSLMEKIRGAIKEDALLEFR
CCCCCEEEHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEFLRKYLNKASI
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA