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The map label for this gene is queA

Identifier: 220932067

GI number: 220932067

Start: 1323877

End: 1324902

Strand: Reverse

Name: queA

Synonym: Hore_12300

Alternate gene names: 220932067

Gene position: 1324902-1323877 (Counterclockwise)

Preceding gene: 220932068

Following gene: 220932066

Centisome position: 51.39

GC content: 38.6

Gene sequence:

>1026_bases
ATGAAGGTTGAGGAATTTGATTATGAACTACCAGAAGAATTAATAGCCCAGAAGCCTTTACCTAACAGAGATGATTCCCG
GTTAATGGTTTTACACCGGAAAACTGGAGAAATTGAAGAAACAGTCTTCAAAAATATTATTAAGTTTCTGGAGCCAGGAG
ATTTACTTGTTTTAAATGATACTAAAGTAATACCTGCCCGGTTGTTTGGTGAAAAAATACCGAGTGGGACCAGGATAGAA
GTCTTATTACTAAAAAGTATAACAGAAGGCCTCTGGGAAGTTCTGGTAAGACCTGGTAGAAGGATGAAGAAAGGTAACCG
GGTTTCTTTTGGTGATGGAAAACTGGTTGGTATTGTTAAAGATTATACCGATTTTGGTGGTAGGATTATGCAATTTGAAT
ATGACGGTGATTTTGATAAGGTTATTGAAGAATTAGGGGAAATGCCACTACCACCGTATATAACCAGGAAAGTTGAAGAC
AAAGGCCGGTATCAGACGGTTTATGCCCGGAAAAAGGGATCTGTAGCTGCCCCTACAGCAGGTTTGCACTTTACTGACCG
ACTTCTAAAGAAAATAAAGGAACAGGGAATTGGAATCATCTATTTGACCCTTCATGTGGGGCTGGGCACTTTTAGACCAG
TACGTGCGGAAAATGTTGAGGACCATAAGATGCATAGTGAGTATTTTGAGGTTTCAACAGATGTGGTCAACAGGATTAAA
GAGACAAAAGAAAAAGGGAAAAAAGTTATTGCTGTCGGTACTACAGTGACCAGGGCCCTTGAATCTGCTGCTGTAGAAAA
AAGACACCTAAAAGCCATGAGGGGCTGGACTGATATTTTTATATACCCTGGTTATAATTTTAAGGTCATAGATGGGCTTA
TTACCAATTTCCATTTACCTAAATCAACTTTATTGATGCTGGTCAGTGCCTTTGCCGGCAAAGATATGGTTATGTCTGCC
TATAAGTTAGCCATCAAAAATAAATATAGATTTTTTAGTTTTGGTGATGCCATGTTAATATTATGA

Upstream 100 bases:

>100_bases
TGCTTACGAACACCTTGATGTAGACTATAAGAATAAGTCGCAATCTCTTTTTAATAATTAATACATGAGTTAATATTGAA
CTAATTATGGAGGTCTGAAA

Downstream 100 bases:

>100_bases
ATCTAAGATCCAGTAAAAGATAGTTTTAAAAGAGGAATTAGAAAAGGAATTACAAATTTGAGATGTACCAGTAAAAGGGG
GATTTATAAGTATATGTCCA

Product: S-adenosylmethionine--tRNA-ribosyltransferase- isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKVEEFDYELPEELIAQKPLPNRDDSRLMVLHRKTGEIEETVFKNIIKFLEPGDLLVLNDTKVIPARLFGEKIPSGTRIE
VLLLKSITEGLWEVLVRPGRRMKKGNRVSFGDGKLVGIVKDYTDFGGRIMQFEYDGDFDKVIEELGEMPLPPYITRKVED
KGRYQTVYARKKGSVAAPTAGLHFTDRLLKKIKEQGIGIIYLTLHVGLGTFRPVRAENVEDHKMHSEYFEVSTDVVNRIK
ETKEKGKKVIAVGTTVTRALESAAVEKRHLKAMRGWTDIFIYPGYNFKVIDGLITNFHLPKSTLLMLVSAFAGKDMVMSA
YKLAIKNKYRFFSFGDAMLIL

Sequences:

>Translated_341_residues
MKVEEFDYELPEELIAQKPLPNRDDSRLMVLHRKTGEIEETVFKNIIKFLEPGDLLVLNDTKVIPARLFGEKIPSGTRIE
VLLLKSITEGLWEVLVRPGRRMKKGNRVSFGDGKLVGIVKDYTDFGGRIMQFEYDGDFDKVIEELGEMPLPPYITRKVED
KGRYQTVYARKKGSVAAPTAGLHFTDRLLKKIKEQGIGIIYLTLHVGLGTFRPVRAENVEDHKMHSEYFEVSTDVVNRIK
ETKEKGKKVIAVGTTVTRALESAAVEKRHLKAMRGWTDIFIYPGYNFKVIDGLITNFHLPKSTLLMLVSAFAGKDMVMSA
YKLAIKNKYRFFSFGDAMLIL
>Mature_341_residues
MKVEEFDYELPEELIAQKPLPNRDDSRLMVLHRKTGEIEETVFKNIIKFLEPGDLLVLNDTKVIPARLFGEKIPSGTRIE
VLLLKSITEGLWEVLVRPGRRMKKGNRVSFGDGKLVGIVKDYTDFGGRIMQFEYDGDFDKVIEELGEMPLPPYITRKVED
KGRYQTVYARKKGSVAAPTAGLHFTDRLLKKIKEQGIGIIYLTLHVGLGTFRPVRAENVEDHKMHSEYFEVSTDVVNRIK
ETKEKGKKVIAVGTTVTRALESAAVEKRHLKAMRGWTDIFIYPGYNFKVIDGLITNFHLPKSTLLMLVSAFAGKDMVMSA
YKLAIKNKYRFFSFGDAMLIL

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=346, Percent_Identity=48.5549132947977, Blast_Score=325, Evalue=3e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_HALOH (B8CXG1)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002508975.1
- ProteinModelPortal:   B8CXG1
- SMR:   B8CXG1
- GeneID:   7313931
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_12300
- HOGENOM:   HBG286076
- OMA:   SEFYRLT
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38823; Mature: 38823

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVEEFDYELPEELIAQKPLPNRDDSRLMVLHRKTGEIEETVFKNIIKFLEPGDLLVLND
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECC
TKVIPARLFGEKIPSGTRIEVLLLKSITEGLWEVLVRPGRRMKKGNRVSFGDGKLVGIVK
CCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHCCCCEEECCCCCEEEEEE
DYTDFGGRIMQFEYDGDFDKVIEELGEMPLPPYITRKVEDKGRYQTVYARKKGSVAAPTA
CHHCCCCEEEEEEECCCHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEEEECCCCCCCCCC
GLHFTDRLLKKIKEQGIGIIYLTLHVGLGTFRPVRAENVEDHKMHSEYFEVSTDVVNRIK
CCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ETKEKGKKVIAVGTTVTRALESAAVEKRHLKAMRGWTDIFIYPGYNFKVIDGLITNFHLP
HHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEHHHHHHHCCCC
KSTLLMLVSAFAGKDMVMSAYKLAIKNKYRFFSFGDAMLIL
HHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCEEEEC
>Mature Secondary Structure
MKVEEFDYELPEELIAQKPLPNRDDSRLMVLHRKTGEIEETVFKNIIKFLEPGDLLVLND
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECC
TKVIPARLFGEKIPSGTRIEVLLLKSITEGLWEVLVRPGRRMKKGNRVSFGDGKLVGIVK
CCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHCCCCEEECCCCCEEEEEE
DYTDFGGRIMQFEYDGDFDKVIEELGEMPLPPYITRKVEDKGRYQTVYARKKGSVAAPTA
CHHCCCCEEEEEEECCCHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEEEECCCCCCCCCC
GLHFTDRLLKKIKEQGIGIIYLTLHVGLGTFRPVRAENVEDHKMHSEYFEVSTDVVNRIK
CCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ETKEKGKKVIAVGTTVTRALESAAVEKRHLKAMRGWTDIFIYPGYNFKVIDGLITNFHLP
HHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEHHHHHHHCCCC
KSTLLMLVSAFAGKDMVMSAYKLAIKNKYRFFSFGDAMLIL
HHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA