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The map label for this gene is thiN [H]

Identifier: 220931847

GI number: 220931847

Start: 1094453

End: 1095100

Strand: Direct

Name: thiN [H]

Synonym: Hore_10040

Alternate gene names: 220931847

Gene position: 1094453-1095100 (Clockwise)

Preceding gene: 220931846

Following gene: 220931848

Centisome position: 42.45

GC content: 35.49

Gene sequence:

>648_bases
ATGGGTGAAAAGAAAGCTGTGATTGCTTTAAACGGCTTCCTGACAGGAAAAAAAGAAGATTATCAAAAATATATCAGGGA
TATAGACCTTGTAATCGGGGCAGATGGTGGGGCTCTTTTATTAAAAAAAATTAATGTAATTCCTGATTTAGTTATAGGTG
ATTTTGATTCCCTGACTGAGAGTGAACTCAACTTTTTTAAAAAACAGGGGGTAACTATAAGAAAATACCCTGTTGAGAAG
GATGAAACTGATGGTGAATTGGCGTTAAATTATTGTATTGAGAAAGGTTATGGTATAGTATATTTTATTGGAGCTCTGGG
AGGCAGGGTAGATCAGCAGCTTGCCAACATATTTTTGCTGGAAATGGCCAATAGATATGGTGTTAAGGCCATTGTTAAAG
AACCTGATGAGGAGATAGGCTTAATTAATGACAGATTATTGATAAGAGATAAACAGGGGGCCAGATTCTCAATACTTCCC
CTGGACGACAGGGTTTCTGGTGTTAGCCTTAAGGGATTTAAGTATCCCCTTAAAAATGCCATTTTGAATCGATATAAAAC
CAGGGGAATAAGTAATAAGATTGTTGAAAATAAAGCAACAGTTAAAGTGGAAAAAGGATTGTTGATGTATATTATTTCAG
AAGAATAA

Upstream 100 bases:

>100_bases
CTTATTTAATACCGGCCCTCCTGTTATCAATGGTTTTAATTATCCCTTTATACAGAAAGTTGATTTTATATAATAATGAA
AATATATGAGGTAGGATAAA

Downstream 100 bases:

>100_bases
AACCCCTTCCTTTAAAATATTATTTAAGTAGAGGAAAGGGGAGGGGGTAATGGAACTAAAAAGTTTGAAACTGGGGGATC
TGGTTTTAAAGAAAGATTCC

Product: thiamine pyrophosphokinase

Products: NA

Alternate protein names: TPK; Thiamine diphosphokinase [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MGEKKAVIALNGFLTGKKEDYQKYIRDIDLVIGADGGALLLKKINVIPDLVIGDFDSLTESELNFFKKQGVTIRKYPVEK
DETDGELALNYCIEKGYGIVYFIGALGGRVDQQLANIFLLEMANRYGVKAIVKEPDEEIGLINDRLLIRDKQGARFSILP
LDDRVSGVSLKGFKYPLKNAILNRYKTRGISNKIVENKATVKVEKGLLMYIISEE

Sequences:

>Translated_215_residues
MGEKKAVIALNGFLTGKKEDYQKYIRDIDLVIGADGGALLLKKINVIPDLVIGDFDSLTESELNFFKKQGVTIRKYPVEK
DETDGELALNYCIEKGYGIVYFIGALGGRVDQQLANIFLLEMANRYGVKAIVKEPDEEIGLINDRLLIRDKQGARFSILP
LDDRVSGVSLKGFKYPLKNAILNRYKTRGISNKIVENKATVKVEKGLLMYIISEE
>Mature_214_residues
GEKKAVIALNGFLTGKKEDYQKYIRDIDLVIGADGGALLLKKINVIPDLVIGDFDSLTESELNFFKKQGVTIRKYPVEKD
ETDGELALNYCIEKGYGIVYFIGALGGRVDQQLANIFLLEMANRYGVKAIVKEPDEEIGLINDRLLIRDKQGARFSILPL
DDRVSGVSLKGFKYPLKNAILNRYKTRGISNKIVENKATVKVEKGLLMYIISEE

Specific function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate [H]

COG id: COG1564

COG function: function code H; Thiamine pyrophosphokinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine pyrophosphokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006282
- InterPro:   IPR007373
- InterPro:   IPR007371 [H]

Pfam domain/function: PF04263 TPK_catalytic [H]

EC number: =2.7.6.2 [H]

Molecular weight: Translated: 24080; Mature: 23949

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEKKAVIALNGFLTGKKEDYQKYIRDIDLVIGADGGALLLKKINVIPDLVIGDFDSLTE
CCCCEEEEEEECEECCCHHHHHHHHHHEEEEEECCCCEEEEEHHCCCCCCEECCCCHHHH
SELNFFKKQGVTIRKYPVEKDETDGELALNYCIEKGYGIVYFIGALGGRVDQQLANIFLL
HHHHHHHHCCCEEEECCCCCCCCCCHHHHEEHHHCCCEEEEEEEHHCCHHHHHHHHHHHH
EMANRYGVKAIVKEPDEEIGLINDRLLIRDKQGARFSILPLDDRVSGVSLKGFKYPLKNA
HHHHHCCEEEEECCCCHHCCEECCEEEEEECCCCEEEEEECCCCCCCEEECCCCCCHHHH
ILNRYKTRGISNKIVENKATVKVEKGLLMYIISEE
HHHHHHHCCCCHHHHCCCCEEEEECCEEEEEEECC
>Mature Secondary Structure 
GEKKAVIALNGFLTGKKEDYQKYIRDIDLVIGADGGALLLKKINVIPDLVIGDFDSLTE
CCCEEEEEEECEECCCHHHHHHHHHHEEEEEECCCCEEEEEHHCCCCCCEECCCCHHHH
SELNFFKKQGVTIRKYPVEKDETDGELALNYCIEKGYGIVYFIGALGGRVDQQLANIFLL
HHHHHHHHCCCEEEECCCCCCCCCCHHHHEEHHHCCCEEEEEEEHHCCHHHHHHHHHHHH
EMANRYGVKAIVKEPDEEIGLINDRLLIRDKQGARFSILPLDDRVSGVSLKGFKYPLKNA
HHHHHCCEEEEECCCCHHCCEECCEEEEEECCCCEEEEEECCCCCCCEEECCCCCCHHHH
ILNRYKTRGISNKIVENKATVKVEKGLLMYIISEE
HHHHHHHCCCCHHHHCCCCEEEEECCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]