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The map label for this gene is purR [H]

Identifier: 220931796

GI number: 220931796

Start: 1028791

End: 1029813

Strand: Direct

Name: purR [H]

Synonym: Hore_09530

Alternate gene names: 220931796

Gene position: 1028791-1029813 (Clockwise)

Preceding gene: 220931795

Following gene: 220931797

Centisome position: 39.9

GC content: 29.72

Gene sequence:

>1023_bases
ATGATTACTATTTATGATGTAGCTAAGGAAGCGGGAGTTTCACCTTCTACAGTATCAAGAGTTTTAAATAATTATAATAA
TGTAACTGAAACAACAAGAAAAAAAGTAGAAGCGGCCTGTAAAAAACTAAAATATGTCCCCAATGCTAATGCCAGTAGCC
TTAAAAAAGATAATACTAAAACATTAGCATTAATAATTCCAGATATAGAGAATCCTTTTTTTATTTCAATTTTAAAAGGA
TTTGATGATAAGGCTAATAAGTTAGGTTATGATACTATTTTATGTAATACGGATGAAAGGTTAGAGAAAGAAAAAGATTA
TATAAAGATGGTATTAAAAAAAAGAATAGATGGTGTAGCTATAAGTACAGTAGGAAAAACGAGTGAACATATAAGTGAAT
TTAGTGATAGAAATATTCCATATATATTATTAGATCGTAAAGTTGAGGGATTAGATGCAGATATTGTATGTGGTGATAAT
TATCAAGGTGCAATAGACTTAGTCAACCATCTTATAAATAATGGACATAAGGATATAGCTATGATTACTGGGCCTTTACA
TGTATCAACTAGTAGAGAGAGATTTGAAGGGTATAAAACTGCTTTAAATAGAGCAGGTATCAGTGTTGAAGAGGATTATA
TTAAAATTGATTATGTAAGTCAAGATTATTCTGGAGAAAAAGCTTATGAAATGACAAAGGAATTATTGGGTTTAAATGAA
CCCCCAACTGCTATATTTGTAGCTAATAATTTAATGGCCCTGGAAGTTTATAAAGCTTTGAAAGAGGAAGGAATATCTGT
ACCTGATGATATTGCGATAGTGTGTTTTGATGATCTTAATTTAATTTATGAAATAGAGCCGTTCTTTACTGTTATGAAGC
AACCTGCTTATACAATGGGAGAAGCTGCGGCAGAGATATTAATTAAAAGAATTGAAATGAGTGATAATGGACATAGAAAA
AGAAAAATAGTTTTTGAACCAGAATTGATAATCAGAAAATCATCCATTAAAGATAGTACGTAA

Upstream 100 bases:

>100_bases
TATTAAGTTTTAACAAACAAAAAATTATTAGTATTATTTAATTATATTTTTTATTTTTTAAAAATATAAAACCGATTTCA
TAATTAAAAGGGTGTTAGTT

Downstream 100 bases:

>100_bases
AATTAAAGATAGTACGTAAAATAAAAAATTAAAAAAATTAAATCAAAAAAATATTTTAAAAAAAGCTTGACTATTTAAAA
TTGATATATTATAATTAAAA

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MITIYDVAKEAGVSPSTVSRVLNNYNNVTETTRKKVEAACKKLKYVPNANASSLKKDNTKTLALIIPDIENPFFISILKG
FDDKANKLGYDTILCNTDERLEKEKDYIKMVLKKRIDGVAISTVGKTSEHISEFSDRNIPYILLDRKVEGLDADIVCGDN
YQGAIDLVNHLINNGHKDIAMITGPLHVSTSRERFEGYKTALNRAGISVEEDYIKIDYVSQDYSGEKAYEMTKELLGLNE
PPTAIFVANNLMALEVYKALKEEGISVPDDIAIVCFDDLNLIYEIEPFFTVMKQPAYTMGEAAAEILIKRIEMSDNGHRK
RKIVFEPELIIRKSSIKDST

Sequences:

>Translated_340_residues
MITIYDVAKEAGVSPSTVSRVLNNYNNVTETTRKKVEAACKKLKYVPNANASSLKKDNTKTLALIIPDIENPFFISILKG
FDDKANKLGYDTILCNTDERLEKEKDYIKMVLKKRIDGVAISTVGKTSEHISEFSDRNIPYILLDRKVEGLDADIVCGDN
YQGAIDLVNHLINNGHKDIAMITGPLHVSTSRERFEGYKTALNRAGISVEEDYIKIDYVSQDYSGEKAYEMTKELLGLNE
PPTAIFVANNLMALEVYKALKEEGISVPDDIAIVCFDDLNLIYEIEPFFTVMKQPAYTMGEAAAEILIKRIEMSDNGHRK
RKIVFEPELIIRKSSIKDST
>Mature_340_residues
MITIYDVAKEAGVSPSTVSRVLNNYNNVTETTRKKVEAACKKLKYVPNANASSLKKDNTKTLALIIPDIENPFFISILKG
FDDKANKLGYDTILCNTDERLEKEKDYIKMVLKKRIDGVAISTVGKTSEHISEFSDRNIPYILLDRKVEGLDADIVCGDN
YQGAIDLVNHLINNGHKDIAMITGPLHVSTSRERFEGYKTALNRAGISVEEDYIKIDYVSQDYSGEKAYEMTKELLGLNE
PPTAIFVANNLMALEVYKALKEEGISVPDDIAIVCFDDLNLIYEIEPFFTVMKQPAYTMGEAAAEILIKRIEMSDNGHRK
RKIVFEPELIIRKSSIKDST

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=337, Percent_Identity=33.8278931750742, Blast_Score=201, Evalue=5e-53,
Organism=Escherichia coli, GI1790369, Length=334, Percent_Identity=33.2335329341317, Blast_Score=197, Evalue=8e-52,
Organism=Escherichia coli, GI1787948, Length=336, Percent_Identity=33.0357142857143, Blast_Score=184, Evalue=6e-48,
Organism=Escherichia coli, GI1788474, Length=335, Percent_Identity=32.5373134328358, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI1787580, Length=325, Percent_Identity=32.6153846153846, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1789202, Length=339, Percent_Identity=30.0884955752212, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1789068, Length=342, Percent_Identity=31.5789473684211, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1786540, Length=348, Percent_Identity=29.5977011494253, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1787906, Length=342, Percent_Identity=28.3625730994152, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI48994940, Length=315, Percent_Identity=28.2539682539683, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1790715, Length=318, Percent_Identity=24.5283018867925, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1786268, Length=314, Percent_Identity=24.203821656051, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790689, Length=328, Percent_Identity=27.1341463414634, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1789456, Length=344, Percent_Identity=26.7441860465116, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790192, Length=258, Percent_Identity=27.5193798449612, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38191; Mature: 38191

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIYDVAKEAGVSPSTVSRVLNNYNNVTETTRKKVEAACKKLKYVPNANASSLKKDNTK
CEEEEECHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCC
TLALIIPDIENPFFISILKGFDDKANKLGYDTILCNTDERLEKEKDYIKMVLKKRIDGVA
EEEEEECCCCCCEEEHHHHCCCHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCEE
ISTVGKTSEHISEFSDRNIPYILLDRKVEGLDADIVCGDNYQGAIDLVNHLINNGHKDIA
EEECCCCHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEEE
MITGPLHVSTSRERFEGYKTALNRAGISVEEDYIKIDYVSQDYSGEKAYEMTKELLGLNE
EEECCEEECCCHHHHHHHHHHHHHCCCCEECCEEEEEEEECCCCCCHHHHHHHHHHCCCC
PPTAIFVANNLMALEVYKALKEEGISVPDDIAIVCFDDLNLIYEIEPFFTVMKQPAYTMG
CCCEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEECHHHHHHHCCCHHHH
EAAAEILIKRIEMSDNGHRKRKIVFEPELIIRKSSIKDST
HHHHHHHHHHHHCCCCCCCCEEEEECCHHEEEECCCCCCC
>Mature Secondary Structure
MITIYDVAKEAGVSPSTVSRVLNNYNNVTETTRKKVEAACKKLKYVPNANASSLKKDNTK
CEEEEECHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCC
TLALIIPDIENPFFISILKGFDDKANKLGYDTILCNTDERLEKEKDYIKMVLKKRIDGVA
EEEEEECCCCCCEEEHHHHCCCHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCEE
ISTVGKTSEHISEFSDRNIPYILLDRKVEGLDADIVCGDNYQGAIDLVNHLINNGHKDIA
EEECCCCHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEEE
MITGPLHVSTSRERFEGYKTALNRAGISVEEDYIKIDYVSQDYSGEKAYEMTKELLGLNE
EEECCEEECCCHHHHHHHHHHHHHCCCCEECCEEEEEEEECCCCCCHHHHHHHHHHCCCC
PPTAIFVANNLMALEVYKALKEEGISVPDDIAIVCFDDLNLIYEIEPFFTVMKQPAYTMG
CCCEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEECHHHHHHHCCCHHHH
EAAAEILIKRIEMSDNGHRKRKIVFEPELIIRKSSIKDST
HHHHHHHHHHHHCCCCCCCCEEEEECCHHEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA