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The map label for this gene is sigG [H]

Identifier: 220931759

GI number: 220931759

Start: 990674

End: 991456

Strand: Direct

Name: sigG [H]

Synonym: Hore_09160

Alternate gene names: 220931759

Gene position: 990674-991456 (Clockwise)

Preceding gene: 220931758

Following gene: 220931760

Centisome position: 38.43

GC content: 39.34

Gene sequence:

>783_bases
ATGGGAAACAAGGTAGAAATAAGTGGAGTAAACACTTCAGAACTACCTGTTTTATCCAATGAAGAAATGCGTAAGCTCTT
TAAGAAAATGCAGCAGGGCGACTGGTCAGCTCGTCAGAAGATAATAAATGGGAACTTAAGATTAGTATTAAGTGTGATAC
AACGTTTTAATAATCGAGGAGAAAATGTTGATGACCTTTTTCAGGTTGGTTGTATTGGTTTAATTAAAGCCATTGACAAC
TTTGATTTAAGTCAAAATGTCAGGTTTTCAACCTATGCTGTTCCCATGATTATCGGTGAGATAAGAAGGTATTTAAGGGA
CAATAATCCTATCAGGGTCAGTCGTTCATTACGGGATACTGCCTATAAAGCCCTGCAGATGAAAGAGAGCTTAAAAAATA
AAAAGTCAGAAGAACCCTCATTAAAAGAAGTTTCTGAAGAACTGGGAATTCCCAGATCTGAGATTATATTTGCGCTGGAT
GCGATTCAGGACCCGATTTCCCTCTTCGAACCTATCTACCATGATGGTGGTGACCCCATATTTGTCATGGACCAGATAAG
TGATGAGAAAACAGAGGATGAAAACTGGCTTGAAGGTATTGCGGTCAGGGAGGGATTAAGAAAGTTAAGTCCCAGGGAAA
AGCTTATTCTGGCTTATAGATTTTATGAAGGCAAGACCCAGATGGAGGTAGCCAATGAGATCGGAATATCTCAGGCCCAG
GTTTCAAGACTGGAAAAGGCTGCATTAAAAAGGTTAAAAAGGCATGTAAAGGAGGATAGATAA

Upstream 100 bases:

>100_bases
ACCAGGGGCTTATGGTTACCTATATTTACATGTATAAATAGGGTAACGATGGCAATAATGATAATAGGAAAAAAATAATA
CCAGTAGCGGGGGGATAAAC

Downstream 100 bases:

>100_bases
CCATGAAAGTATTGAAAGTTATAACCGGACTAATATTTTTAACTGTTGTATTATTGAGTATAAATGCAAATAGCGCCTAT
GTAATTAAAGATACTTCTGA

Product: sporulation sigma factor SigG

Products: NA

Alternate protein names: Stage III sporulation protein G [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MGNKVEISGVNTSELPVLSNEEMRKLFKKMQQGDWSARQKIINGNLRLVLSVIQRFNNRGENVDDLFQVGCIGLIKAIDN
FDLSQNVRFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRDTAYKALQMKESLKNKKSEEPSLKEVSEELGIPRSEIIFALD
AIQDPISLFEPIYHDGGDPIFVMDQISDEKTEDENWLEGIAVREGLRKLSPREKLILAYRFYEGKTQMEVANEIGISQAQ
VSRLEKAALKRLKRHVKEDR

Sequences:

>Translated_260_residues
MGNKVEISGVNTSELPVLSNEEMRKLFKKMQQGDWSARQKIINGNLRLVLSVIQRFNNRGENVDDLFQVGCIGLIKAIDN
FDLSQNVRFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRDTAYKALQMKESLKNKKSEEPSLKEVSEELGIPRSEIIFALD
AIQDPISLFEPIYHDGGDPIFVMDQISDEKTEDENWLEGIAVREGLRKLSPREKLILAYRFYEGKTQMEVANEIGISQAQ
VSRLEKAALKRLKRHVKEDR
>Mature_259_residues
GNKVEISGVNTSELPVLSNEEMRKLFKKMQQGDWSARQKIINGNLRLVLSVIQRFNNRGENVDDLFQVGCIGLIKAIDNF
DLSQNVRFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRDTAYKALQMKESLKNKKSEEPSLKEVSEELGIPRSEIIFALDA
IQDPISLFEPIYHDGGDPIFVMDQISDEKTEDENWLEGIAVREGLRKLSPREKLILAYRFYEGKTQMEVANEIGISQAQV
SRLEKAALKRLKRHVKEDR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes in the forespore [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=255, Percent_Identity=30.9803921568627, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1789448, Length=251, Percent_Identity=29.8804780876494, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1789871, Length=255, Percent_Identity=28.6274509803922, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788231, Length=201, Percent_Identity=29.3532338308458, Blast_Score=71, Evalue=6e-14,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014212
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29869; Mature: 29738

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNKVEISGVNTSELPVLSNEEMRKLFKKMQQGDWSARQKIINGNLRLVLSVIQRFNNRG
CCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHCCC
ENVDDLFQVGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRDT
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHH
AYKALQMKESLKNKKSEEPSLKEVSEELGIPRSEIIFALDAIQDPISLFEPIYHDGGDPI
HHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCE
FVMDQISDEKTEDENWLEGIAVREGLRKLSPREKLILAYRFYEGKTQMEVANEIGISQAQ
EEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHEEEEEEEECCCHHHHHHHHHCCCHHH
VSRLEKAALKRLKRHVKEDR
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GNKVEISGVNTSELPVLSNEEMRKLFKKMQQGDWSARQKIINGNLRLVLSVIQRFNNRG
CCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHCCC
ENVDDLFQVGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRDT
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHH
AYKALQMKESLKNKKSEEPSLKEVSEELGIPRSEIIFALDAIQDPISLFEPIYHDGGDPI
HHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCE
FVMDQISDEKTEDENWLEGIAVREGLRKLSPREKLILAYRFYEGKTQMEVANEIGISQAQ
EEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHEEEEEEEECCCHHHHHHHHHCCCHHH
VSRLEKAALKRLKRHVKEDR
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2497052; 2459711; 9384377 [H]