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The map label for this gene is sigE [H]

Identifier: 220931758

GI number: 220931758

Start: 989236

End: 989973

Strand: Direct

Name: sigE [H]

Synonym: Hore_09150

Alternate gene names: 220931758

Gene position: 989236-989973 (Clockwise)

Preceding gene: 220931757

Following gene: 220931759

Centisome position: 38.37

GC content: 35.91

Gene sequence:

>738_bases
ATGATTAAAAGGTTTTATTTCCTGTTAAAGTTTAAAATACAAATATTATGGATTAAGTCCCTTCAGAAATTGGGATTATA
TAAGTCTCCGGTTTATTACCTGGGTAGTAGTGAAACCCTGCCACCTCCATTGGAGGAAGATGAGGAAGATTACCTTTTGA
AAAGGTTGAGTGAGGGGGATAATAGTGTCCGGCAGTTGCTGATAAAACACAACCTTAGACTGGTTGTTTATATTGCCAGG
AAATTTAGTAATACCGGTATTGATATTGAGGACCTGGTTTCAATAGGAACTATTGGCTTGATCAAAGCGGTTAAGACCTT
TGATGTTAACAAAAATATTAAACTGGCTACCTACGCTTCCCGGTGTATTGAGAATGAAATACTTATGTATTTGAGGAAAA
ATAGCCGTAAGAAGGCAGAAATTTCGTTCGATGACCCCCTGAATATTGACTGGGATGGTAATGAATTAAAACTGTCAGAT
GTTATGGGGACTGAAGGTGATATAATTTACAGGAATATAGAATCAGAAATTGACTGGGAACTATTGAAAGATGCCATGAA
AACATTAAGTGATAGGGAGAAAAAAATCCTTATATTGAGATTTGGACTTTTAAATTCAGGAGAAACAAAGACCCAGAAGG
ATGTAGCTGATTTACTGGGAATATCCCAGTCATATATATCCAGGCTTGAAAAAAGAATTATAAGAAAATTAAAGAGAGAA
GTGGCCAGGATGTGTTAG

Upstream 100 bases:

>100_bases
AATTGGTTTATCCGGACATAAACTTGATGATGAGGATAGTTATCAGGTTTTACTTCATCCTCAACTTATAAGACTCCCGG
TGTAAGGAGGTATTAAGATT

Downstream 100 bases:

>100_bases
TGCTCTTTGTAAAATTAAGTAAAAGCCCAGCCGATAACTAATCGGTTGGGCTTTAATTTTAATTAAATAAGGGACTGGCT
TTGCTAAATAAGGAAAAATA

Product: sporulation sigma factor SigE

Products: NA

Alternate protein names: P31; Sigma-29; Stage II sporulation protein GB [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MIKRFYFLLKFKIQILWIKSLQKLGLYKSPVYYLGSSETLPPPLEEDEEDYLLKRLSEGDNSVRQLLIKHNLRLVVYIAR
KFSNTGIDIEDLVSIGTIGLIKAVKTFDVNKNIKLATYASRCIENEILMYLRKNSRKKAEISFDDPLNIDWDGNELKLSD
VMGTEGDIIYRNIESEIDWELLKDAMKTLSDREKKILILRFGLLNSGETKTQKDVADLLGISQSYISRLEKRIIRKLKRE
VARMC

Sequences:

>Translated_245_residues
MIKRFYFLLKFKIQILWIKSLQKLGLYKSPVYYLGSSETLPPPLEEDEEDYLLKRLSEGDNSVRQLLIKHNLRLVVYIAR
KFSNTGIDIEDLVSIGTIGLIKAVKTFDVNKNIKLATYASRCIENEILMYLRKNSRKKAEISFDDPLNIDWDGNELKLSD
VMGTEGDIIYRNIESEIDWELLKDAMKTLSDREKKILILRFGLLNSGETKTQKDVADLLGISQSYISRLEKRIIRKLKRE
VARMC
>Mature_245_residues
MIKRFYFLLKFKIQILWIKSLQKLGLYKSPVYYLGSSETLPPPLEEDEEDYLLKRLSEGDNSVRQLLIKHNLRLVVYIAR
KFSNTGIDIEDLVSIGTIGLIKAVKTFDVNKNIKLATYASRCIENEILMYLRKNSRKKAEISFDDPLNIDWDGNELKLSD
VMGTEGDIIYRNIESEIDWELLKDAMKTLSDREKKILILRFGLLNSGETKTQKDVADLLGISQSYISRLEKRIIRKLKRE
VARMC

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=95, Percent_Identity=40, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789871, Length=120, Percent_Identity=36.6666666666667, Blast_Score=67, Evalue=8e-13,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014200
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 28486; Mature: 28486

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRFYFLLKFKIQILWIKSLQKLGLYKSPVYYLGSSETLPPPLEEDEEDYLLKRLSEGD
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCH
NSVRQLLIKHNLRLVVYIARKFSNTGIDIEDLVSIGTIGLIKAVKTFDVNKNIKLATYAS
HHHHHHHHHCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHH
RCIENEILMYLRKNSRKKAEISFDDPLNIDWDGNELKLSDVMGTEGDIIYRNIESEIDWE
HHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEEEHHHCCCCCCEEEECCCCCCCHH
LLKDAMKTLSDREKKILILRFGLLNSGETKTQKDVADLLGISQSYISRLEKRIIRKLKRE
HHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VARMC
HHHCC
>Mature Secondary Structure
MIKRFYFLLKFKIQILWIKSLQKLGLYKSPVYYLGSSETLPPPLEEDEEDYLLKRLSEGD
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCH
NSVRQLLIKHNLRLVVYIARKFSNTGIDIEDLVSIGTIGLIKAVKTFDVNKNIKLATYAS
HHHHHHHHHCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHH
RCIENEILMYLRKNSRKKAEISFDDPLNIDWDGNELKLSDVMGTEGDIIYRNIESEIDWE
HHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEEEHHHCCCCCCEEEECCCCCCCHH
LLKDAMKTLSDREKKILILRFGLLNSGETKTQKDVADLLGISQSYISRLEKRIIRKLKRE
HHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VARMC
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6438529; 9384377; 3104904; 2106671 [H]