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The map label for this gene is iscS [H]

Identifier: 220931731

GI number: 220931731

Start: 956038

End: 957216

Strand: Direct

Name: iscS [H]

Synonym: Hore_08880

Alternate gene names: 220931731

Gene position: 956038-957216 (Clockwise)

Preceding gene: 220931730

Following gene: 220931732

Centisome position: 37.08

GC content: 40.8

Gene sequence:

>1179_bases
ATGAAGAATATCTATTTAGATAATGCTGCAACCACACCGGTTGCACCTGAAGTTATTAAAGTTATGGAACCCTATTTTAA
TATATACTACGGAAATCCCTCAAGTGTCCACACCCCTGGGCAGGATGCTGCCAGAGCTGTAAGTGAAGCCCGGGAGAAAG
TTGCAGAATTAATTGGAGCAAGAGATGAAAGGGAGATAATTTTTACCAGTGGTGGTACTGAAGCCGATAACCTGGCCATA
AAAGGAGTGGCTATGTCTTTACAGGACAGGGGTAAACACATTATAACCTCAAGTGTCGAACACCATGCCGTTCTTCATAC
CTGTGAATATCTGGAGAAATACCTTGGTTTTGATGTAACCTATTTACCTGTTGATGAAAAAGGGTTTGTAGACCCTTCTA
AGGTGGAAGAGGCTATAAGAGAAGATACGATTTTGATATCAATTATGTTGGCGAACAATGAGATTGGGACCATTCAGCCA
GTTAAAGAGATCGCTAAAATAGCCAATGAACATGATATTTATTTCCATACTGATGCTGTTCAGGCTATCGGTCAGATACC
GGTAGATGTAGAAAAACTGGGAGTTGATTTATTATCTCTATCCGGTCATAAGTTTAATGGTCCTAAAGGGGTAGGAGCCC
TGTATATAAGAAAGGGTGTTAAATTAGCACCCCAGATGTCCGGGGGTGCTCAGGAAAGGAGAAGGAGAGCTGGAACGGAG
AATGTTCCCGGCATTGTTGGTCTGGGTAAAGCTGCTGAAATGGCTGCACATAACCTGGAAGAAAAACGTCTTAAGCTGAA
AAAACTTCGTGATAAATTAATAAACGGCATTGAAAATGAAATTGATGAAGTATATTTAAATGGTCCCCGGGGGGAAGATA
GGCTTCCCAATAATGTTAATTTCTGTTTTAGGTATATTGAAGGGGAATCGATTCTATTAAATCTCGATATGATGGGGATT
GCGGGATCAAGTGGTTCTGCCTGTACTTCAGGTTCTCTGGATCCTTCCCATGTTTTACTGGCTATAGGTAGGCCTCATGA
GATTGCCCATGGCTCTTTAAGATTAACCCTGGGATATAACAATACCGAAGAAGAAGTTGATTATGTTCTTGAAGTATTAC
CGGGGATTATAAAAAAATTAAGGGCTATGTCTCCATTGTTTGATTCAGCTTCTGAGTAA

Upstream 100 bases:

>100_bases
GTTACTTTAAGAGACTTACAGGAAAGGGCAAAAGAACTAATACGTAATAAAAATAAAGGTTTGATGTATCATATCTGAAT
ATTTTAAGGGGTGTTTTTAT

Downstream 100 bases:

>100_bases
GTTCTCAGCATAGTTTTTAAAATGGAGGAGATGATAAAAATGTATTCTGAAAAAGTAATGGAACACTTCAATAATCCCAG
AAATGTCGGTGAGATAAAGG

Product: class V aminotransferase

Products: NA

Alternate protein names: NifS protein homolog [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MKNIYLDNAATTPVAPEVIKVMEPYFNIYYGNPSSVHTPGQDAARAVSEAREKVAELIGARDEREIIFTSGGTEADNLAI
KGVAMSLQDRGKHIITSSVEHHAVLHTCEYLEKYLGFDVTYLPVDEKGFVDPSKVEEAIREDTILISIMLANNEIGTIQP
VKEIAKIANEHDIYFHTDAVQAIGQIPVDVEKLGVDLLSLSGHKFNGPKGVGALYIRKGVKLAPQMSGGAQERRRRAGTE
NVPGIVGLGKAAEMAAHNLEEKRLKLKKLRDKLINGIENEIDEVYLNGPRGEDRLPNNVNFCFRYIEGESILLNLDMMGI
AGSSGSACTSGSLDPSHVLLAIGRPHEIAHGSLRLTLGYNNTEEEVDYVLEVLPGIIKKLRAMSPLFDSASE

Sequences:

>Translated_392_residues
MKNIYLDNAATTPVAPEVIKVMEPYFNIYYGNPSSVHTPGQDAARAVSEAREKVAELIGARDEREIIFTSGGTEADNLAI
KGVAMSLQDRGKHIITSSVEHHAVLHTCEYLEKYLGFDVTYLPVDEKGFVDPSKVEEAIREDTILISIMLANNEIGTIQP
VKEIAKIANEHDIYFHTDAVQAIGQIPVDVEKLGVDLLSLSGHKFNGPKGVGALYIRKGVKLAPQMSGGAQERRRRAGTE
NVPGIVGLGKAAEMAAHNLEEKRLKLKKLRDKLINGIENEIDEVYLNGPRGEDRLPNNVNFCFRYIEGESILLNLDMMGI
AGSSGSACTSGSLDPSHVLLAIGRPHEIAHGSLRLTLGYNNTEEEVDYVLEVLPGIIKKLRAMSPLFDSASE
>Mature_392_residues
MKNIYLDNAATTPVAPEVIKVMEPYFNIYYGNPSSVHTPGQDAARAVSEAREKVAELIGARDEREIIFTSGGTEADNLAI
KGVAMSLQDRGKHIITSSVEHHAVLHTCEYLEKYLGFDVTYLPVDEKGFVDPSKVEEAIREDTILISIMLANNEIGTIQP
VKEIAKIANEHDIYFHTDAVQAIGQIPVDVEKLGVDLLSLSGHKFNGPKGVGALYIRKGVKLAPQMSGGAQERRRRAGTE
NVPGIVGLGKAAEMAAHNLEEKRLKLKKLRDKLINGIENEIDEVYLNGPRGEDRLPNNVNFCFRYIEGESILLNLDMMGI
AGSSGSACTSGSLDPSHVLLAIGRPHEIAHGSLRLTLGYNNTEEEVDYVLEVLPGIIKKLRAMSPLFDSASE

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=392, Percent_Identity=48.9795918367347, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI156713448, Length=416, Percent_Identity=39.4230769230769, Blast_Score=251, Evalue=6e-67,
Organism=Escherichia coli, GI48994898, Length=390, Percent_Identity=48.974358974359, Blast_Score=355, Evalue=3e-99,
Organism=Escherichia coli, GI1787970, Length=398, Percent_Identity=27.8894472361809, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1789175, Length=388, Percent_Identity=26.0309278350515, Blast_Score=115, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI25143064, Length=393, Percent_Identity=47.5826972010178, Blast_Score=347, Evalue=9e-96,
Organism=Caenorhabditis elegans, GI17533177, Length=319, Percent_Identity=32.6018808777429, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI193211090, Length=374, Percent_Identity=27.0053475935829, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319831, Length=392, Percent_Identity=46.9387755102041, Blast_Score=342, Evalue=5e-95,
Organism=Drosophila melanogaster, GI20129463, Length=392, Percent_Identity=46.9387755102041, Blast_Score=345, Evalue=4e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42838; Mature: 42838

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIYLDNAATTPVAPEVIKVMEPYFNIYYGNPSSVHTPGQDAARAVSEAREKVAELIGA
CCCEEECCCCCCCCCHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
RDEREIIFTSGGTEADNLAIKGVAMSLQDRGKHIITSSVEHHAVLHTCEYLEKYLGFDVT
CCCCEEEEECCCCCCCCEEEEHEEEEHHHCCCEEECCCHHHHHHHHHHHHHHHHHCCCEE
YLPVDEKGFVDPSKVEEAIREDTILISIMLANNEIGTIQPVKEIAKIANEHDIYFHTDAV
EEECCCCCCCCHHHHHHHHHCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEECHHH
QAIGQIPVDVEKLGVDLLSLSGHKFNGPKGVGALYIRKGVKLAPQMSGGAQERRRRAGTE
HHHCCCCCCHHHHCCHHEECCCCCCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHCCCC
NVPGIVGLGKAAEMAAHNLEEKRLKLKKLRDKLINGIENEIDEVYLNGPRGEDRLPNNVN
CCCCEEECCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCE
FCFRYIEGESILLNLDMMGIAGSSGSACTSGSLDPSHVLLAIGRPHEIAHGSLRLTLGYN
EEEEEECCCEEEEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCEECCCEEEEEECCC
NTEEEVDYVLEVLPGIIKKLRAMSPLFDSASE
CCHHHHHHHHHHHHHHHHHHHHHCHHHCCCCC
>Mature Secondary Structure
MKNIYLDNAATTPVAPEVIKVMEPYFNIYYGNPSSVHTPGQDAARAVSEAREKVAELIGA
CCCEEECCCCCCCCCHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
RDEREIIFTSGGTEADNLAIKGVAMSLQDRGKHIITSSVEHHAVLHTCEYLEKYLGFDVT
CCCCEEEEECCCCCCCCEEEEHEEEEHHHCCCEEECCCHHHHHHHHHHHHHHHHHCCCEE
YLPVDEKGFVDPSKVEEAIREDTILISIMLANNEIGTIQPVKEIAKIANEHDIYFHTDAV
EEECCCCCCCCHHHHHHHHHCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEECHHH
QAIGQIPVDVEKLGVDLLSLSGHKFNGPKGVGALYIRKGVKLAPQMSGGAQERRRRAGTE
HHHCCCCCCHHHHCCHHEECCCCCCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHCCCC
NVPGIVGLGKAAEMAAHNLEEKRLKLKKLRDKLINGIENEIDEVYLNGPRGEDRLPNNVN
CCCCEEECCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCE
FCFRYIEGESILLNLDMMGIAGSSGSACTSGSLDPSHVLLAIGRPHEIAHGSLRLTLGYN
EEEEEECCCEEEEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCEECCCEEEEEECCC
NTEEEVDYVLEVLPGIIKKLRAMSPLFDSASE
CCHHHHHHHHHHHHHHHHHHHHHCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10930739 [H]