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The map label for this gene is thrC [H]

Identifier: 220931647

GI number: 220931647

Start: 862764

End: 863819

Strand: Direct

Name: thrC [H]

Synonym: Hore_08030

Alternate gene names: 220931647

Gene position: 862764-863819 (Clockwise)

Preceding gene: 220931646

Following gene: 220931648

Centisome position: 33.46

GC content: 44.03

Gene sequence:

>1056_bases
ATGAAAAAATGGCCTGGAATAATTGAAGCTTATAGAGAATTTATGCCCCTTAAGGAAACAACAAAACCGGTAACCCTGCA
GGAGGGAAATACCCCTTTAATATATGCTGACAGGTTAAGTGAAAAACTTGGTATTCAACTATACTTAAAATATGATGGAG
CCAATCCAACTGGTTCTTTTAAAGATAGGGGTATGACTCTGGCTGTAACTAAAGCTATAGAAGATGGATCAGAGGCTATT
ATGTGTGCATCAACAGGTAATACTTCTGCAGCAGCAGCAGCCTATGGGGCCAGAAGTGGCCTTAAGACAGCTGTAGTTAT
TCCTGAAGGAAAGATAGCCCTGGGGAAACTGGCCCAGGCCTTAATGCATGGGGCTGAGGTAATACCTATCAAGGGAAATT
TTGATGATGCCCTGGCTATAGTTAAGGAGATTACCAGTAAATATCCCATAACACTTGTTAATTCTTTAAATCCATACCGG
ATAGAAGGCCAGAAAAGTGGTGCCTTTGAGGTCTGTGATCAACTCGGAAACGCCCCTGATGTTCTGGCTATACCGGTTGG
TAATGCCGGAAATATAACTGCCTACTGGAAGGGTTTTAAAGAATATTACCGGGCTAAAAAGATAGAGTCATTGCCCCGTA
TGCTTGGTTTTGAAGCTGAAGGTTCAGCAGCCATAGCTAAAAATACTGTAATCAGTGAACCGGAAACAATTGCTACTGCA
ATAAGAATCGGTAATCCAGCCAGCTGGGATAAAGCTGTTAAAGCTGCTGAAGAATCCCGGGGTCTTATTGATTATGTAAC
AGATAATGAGATTATTGAGACCTACAGGGAAATTGCCTCAGAGGTTGGTATATTTGCTGAGCCGGCGTCGGCCGCTTCAG
TGGCAGGGGTTAAAAAAATGGTGGCCCGGAGTGTGATCAAGCCCGGGAGTCAGGTTGTGGCTGTCTTAACCGGTCACGGG
CTTAAAGACCCAACCACTGCAATTGATACCATCAAAAAACCAGAACCTGTTTCCGCAGAGGTAGAGGCGGTTCTTGAGAA
AATGGGAACATGGTAG

Upstream 100 bases:

>100_bases
CTGTTAAAGAGCAATTTATTAATAAGTCATTAAAGGAATTAAAGAAGATTGAAGATGTTGTTAGCATTGATAGTTTAATA
AGGGTTGAGGAGGAATCAAT

Downstream 100 bases:

>100_bases
TAATACAGCGAATGGAGTTGGTATTATGGCTTTAATAGTACAAAAATACGGTGGTACATCGGTGGGAACTATTGAAAGGA
TTAAAAATGTTGCCGACCGT

Product: threonine synthase

Products: NA

Alternate protein names: TS [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MKKWPGIIEAYREFMPLKETTKPVTLQEGNTPLIYADRLSEKLGIQLYLKYDGANPTGSFKDRGMTLAVTKAIEDGSEAI
MCASTGNTSAAAAAYGARSGLKTAVVIPEGKIALGKLAQALMHGAEVIPIKGNFDDALAIVKEITSKYPITLVNSLNPYR
IEGQKSGAFEVCDQLGNAPDVLAIPVGNAGNITAYWKGFKEYYRAKKIESLPRMLGFEAEGSAAIAKNTVISEPETIATA
IRIGNPASWDKAVKAAEESRGLIDYVTDNEIIETYREIASEVGIFAEPASAASVAGVKKMVARSVIKPGSQVVAVLTGHG
LKDPTTAIDTIKKPEPVSAEVEAVLEKMGTW

Sequences:

>Translated_351_residues
MKKWPGIIEAYREFMPLKETTKPVTLQEGNTPLIYADRLSEKLGIQLYLKYDGANPTGSFKDRGMTLAVTKAIEDGSEAI
MCASTGNTSAAAAAYGARSGLKTAVVIPEGKIALGKLAQALMHGAEVIPIKGNFDDALAIVKEITSKYPITLVNSLNPYR
IEGQKSGAFEVCDQLGNAPDVLAIPVGNAGNITAYWKGFKEYYRAKKIESLPRMLGFEAEGSAAIAKNTVISEPETIATA
IRIGNPASWDKAVKAAEESRGLIDYVTDNEIIETYREIASEVGIFAEPASAASVAGVKKMVARSVIKPGSQVVAVLTGHG
LKDPTTAIDTIKKPEPVSAEVEAVLEKMGTW
>Mature_351_residues
MKKWPGIIEAYREFMPLKETTKPVTLQEGNTPLIYADRLSEKLGIQLYLKYDGANPTGSFKDRGMTLAVTKAIEDGSEAI
MCASTGNTSAAAAAYGARSGLKTAVVIPEGKIALGKLAQALMHGAEVIPIKGNFDDALAIVKEITSKYPITLVNSLNPYR
IEGQKSGAFEVCDQLGNAPDVLAIPVGNAGNITAYWKGFKEYYRAKKIESLPRMLGFEAEGSAAIAKNTVISEPETIATA
IRIGNPASWDKAVKAAEESRGLIDYVTDNEIIETYREIASEVGIFAEPASAASVAGVKKMVARSVIKPGSQVVAVLTGHG
LKDPTTAIDTIKKPEPVSAEVEAVLEKMGTW

Specific function: Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine [H]

COG id: COG0498

COG function: function code E; Threonine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790207, Length=311, Percent_Identity=27.6527331189711, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI2367138, Length=313, Percent_Identity=25.5591054313099, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786185, Length=291, Percent_Identity=25.7731958762887, Blast_Score=62, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322631, Length=268, Percent_Identity=25.3731343283582, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR004450 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.3.1 [H]

Molecular weight: Translated: 37436; Mature: 37436

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWPGIIEAYREFMPLKETTKPVTLQEGNTPLIYADRLSEKLGIQLYLKYDGANPTGSF
CCCCCHHHHHHHHHCCHHHCCCCEEEECCCCCEEEHHHHHCCCCEEEEEEECCCCCCCCH
KDRGMTLAVTKAIEDGSEAIMCASTGNTSAAAAAYGARSGLKTAVVIPEGKIALGKLAQA
HHCCEEEEEEEHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
LMHGAEVIPIKGNFDDALAIVKEITSKYPITLVNSLNPYRIEGQKSGAFEVCDQLGNAPD
HHCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCHHHHHHHHCCCCC
VLAIPVGNAGNITAYWKGFKEYYRAKKIESLPRMLGFEAEGSAAIAKNTVISEPETIATA
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCHHHHHE
IRIGNPASWDKAVKAAEESRGLIDYVTDNEIIETYREIASEVGIFAEPASAASVAGVKKM
EECCCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHH
VARSVIKPGSQVVAVLTGHGLKDPTTAIDTIKKPEPVSAEVEAVLEKMGTW
HHHHHHCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKKWPGIIEAYREFMPLKETTKPVTLQEGNTPLIYADRLSEKLGIQLYLKYDGANPTGSF
CCCCCHHHHHHHHHCCHHHCCCCEEEECCCCCEEEHHHHHCCCCEEEEEEECCCCCCCCH
KDRGMTLAVTKAIEDGSEAIMCASTGNTSAAAAAYGARSGLKTAVVIPEGKIALGKLAQA
HHCCEEEEEEEHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
LMHGAEVIPIKGNFDDALAIVKEITSKYPITLVNSLNPYRIEGQKSGAFEVCDQLGNAPD
HHCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCHHHHHHHHCCCCC
VLAIPVGNAGNITAYWKGFKEYYRAKKIESLPRMLGFEAEGSAAIAKNTVISEPETIATA
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCHHHHHE
IRIGNPASWDKAVKAAEESRGLIDYVTDNEIIETYREIASEVGIFAEPASAASVAGVKKM
EECCCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHH
VARSVIKPGSQVVAVLTGHGLKDPTTAIDTIKKPEPVSAEVEAVLEKMGTW
HHHHHHCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3098560; 9384377; 3139660 [H]