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The map label for this gene is pnp

Identifier: 220931634

GI number: 220931634

Start: 848183

End: 850300

Strand: Direct

Name: pnp

Synonym: Hore_07900

Alternate gene names: 220931634

Gene position: 848183-850300 (Clockwise)

Preceding gene: 220931633

Following gene: 220931635

Centisome position: 32.9

GC content: 40.23

Gene sequence:

>2118_bases
ATGCATAAAGATTGGACAATCGATGTTGCCGGAGGAAAGATGAAATTTGAGACTGGCAAATATGCTAAACAAGCAAATGG
TTCTGTAGTGGCACGATATGGTGATACTACAGTACTGGTCACTGCGACCATGTCTGAACCCCGGGAAGGTATTGATTACT
TTCCATTAATGGTTAATTATGAAGAAAGGGTATATGCAATTGGGAAAATACCGGGGAGTATTACAAGAAGGGAAGGAAGA
CCGAGGGATGTAGCTACTTTAGCGGCCCGTTTAATAGATAGACCGTTGCGGCCTCTTTTCCCTGAAGGATTTAGACATGA
TGTACAGATTATCGCTACAGTTCTTTCTGTAGATAATGACTGTGAGCCAGATATACTGGCCCTGAATGGTGCCTCAGTTG
CTCTTACCCTATCTGATATTCCTTTTGACGGGCCCATTGGTGGTGTTAAAGTAGGTCTAGTTGACGGTGAGCTTGTTATT
AATCCCGATGAGGAAGAAAGGGAAAAAAGTAAGCTTGACCTGACGGTAGCTGGAACCAGGGATGCCGTTTTAATGGTTGA
AGCAGGAGCAAATGAAGTTTCTGAAGATGTTATGCTTGATGCTATAGAACTTGCCCACCAGGAGATTAAAAGACTGGTGC
TTTTACAGGAAGAAATAGGGGAAGAAGCCGGGAAAAAGAAATTTGAATTTACAAAAGATGAAATTACTCCTGAACTAGAT
GAAGAAATAAGGAAATATATTAGCTCTGATATGGAAAATGCGTTGAGAATTCCAGAGAAACTGGAACGTAACGCAAAAGT
TGATGAAATCAAGGAAAATACTTTACAGTACTTTGAAGACATGTTTGAAAATAATGGCCTTGACAATGAAGAAAAAAATA
AACAGTTGAAAATGGTAGAAAGAACAATTGAGAAGGTTATGAAGGAAAAAGTCAGGAAAATGATAATAGAAGAAGGAATC
AGGCCTGATGGAAGGAAACCTGATGAAATACGACCTATATGGTGTGAGGTTGGTACTTTACCACGGGTCCATGGTTCCGG
GGTATTTACACGTGGTCAAACCCAGGCTTTAAGTGTTGTCACTCTGGGAGCTACGTCTGATGAACAGATCCTATTTGGAC
TCGGTGAAGAAGAAACAAAGCGGTATATGCATCATTACAATTTCCCCCCATATAGTGTTGGCGAAACAAGCCCTTTAAGA
TCTCCGGGTCGTAGAGAGATAGGACATGGAGCCCTTGGTGAAAGGGCCCTACAGCCTGTGATACCTGATCAGGAAGAATT
TCCCTATACAATCAGGGTTGTCTCCGAAGTCCTCGAGTCTAATGGTTCAACTTCACAGGCCAGTATTTGTGGTAGTACCC
TGGCTTTAATGGATGCTGGTGTCCCAATAAAGGAACCGGTAGCTGGAATTGCCATGGGATTGTTAAAAGAAGATGAAAAA
GTTGTTATTCTTTCCGATATACAGGGTCTGGAAGATTTTTATGGAGATATGGATTTTAAGGTTGCCGGTACCAGAAATGG
TATTACTGCCTTACAGATGGATATTAAAATTCATGGAATTTCCAAGGAGATCCTGAAAAAGGCCCTTAAAAGAGCAAGAG
AAGGTAGATTGTACATTTTAGATAAAATGTTACAGGTTATTGATAAGCCACGCCCTGAATTATCACCATATGCCCCTCTA
ATGATTACCATGAAGGTTTCACCGGACAAAATAAGGCACATTATTGGGCCTGGTGGTAAAATAATTAACAAGATAATTGA
TGAAACTGGCGTTGAAATAGATATCGATGATGATGGTTCAGTTTATATTCTGGCCCAGGATCAGGAAAGTGGTAACAGGG
CTAAAGAGATAATTAATAAACTGACAAAAGAGGTTGAAGTTGGAGATATATATGAAGGAAGGGTAAAAAAGATTACCAAC
TTTGGGGCTTTTGTTGAGATATTACCCGGGCGTGAAGGGCTGGTTCACATTTCTGAGCTTGCTGACCACCATGTAAAAAA
AGTAGAAGATATTGTTAAAATAGGTGACAGAATACCTGTAAAGGTTATTGAAATAGATGAACTGGGAAGGATAAATCTTT
CAAGAAAACGGGCCCTGAAGGAACAAAAAAAAGAATAA

Upstream 100 bases:

>100_bases
AAACAAAAAAGTACATATTTTTGTTTTTATTAATATTCAAAATTTTAATTTCATGAAGTACAGGTTAATCAGAAAGATGT
AAAGTTAAGGAGGATTATTT

Downstream 100 bases:

>100_bases
AAATAAAAATATAAAGGGATTAAAAGGCATAAATTCATTAAACATAAACTAGGGCCCTAGTTTATGTTTTTTTATTATCA
CAAAATTAGAGCAGGTGATA

Product: Polyribonucleotide nucleotidyltransferase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MHKDWTIDVAGGKMKFETGKYAKQANGSVVARYGDTTVLVTATMSEPREGIDYFPLMVNYEERVYAIGKIPGSITRREGR
PRDVATLAARLIDRPLRPLFPEGFRHDVQIIATVLSVDNDCEPDILALNGASVALTLSDIPFDGPIGGVKVGLVDGELVI
NPDEEEREKSKLDLTVAGTRDAVLMVEAGANEVSEDVMLDAIELAHQEIKRLVLLQEEIGEEAGKKKFEFTKDEITPELD
EEIRKYISSDMENALRIPEKLERNAKVDEIKENTLQYFEDMFENNGLDNEEKNKQLKMVERTIEKVMKEKVRKMIIEEGI
RPDGRKPDEIRPIWCEVGTLPRVHGSGVFTRGQTQALSVVTLGATSDEQILFGLGEEETKRYMHHYNFPPYSVGETSPLR
SPGRREIGHGALGERALQPVIPDQEEFPYTIRVVSEVLESNGSTSQASICGSTLALMDAGVPIKEPVAGIAMGLLKEDEK
VVILSDIQGLEDFYGDMDFKVAGTRNGITALQMDIKIHGISKEILKKALKRAREGRLYILDKMLQVIDKPRPELSPYAPL
MITMKVSPDKIRHIIGPGGKIINKIIDETGVEIDIDDDGSVYILAQDQESGNRAKEIINKLTKEVEVGDIYEGRVKKITN
FGAFVEILPGREGLVHISELADHHVKKVEDIVKIGDRIPVKVIEIDELGRINLSRKRALKEQKKE

Sequences:

>Translated_705_residues
MHKDWTIDVAGGKMKFETGKYAKQANGSVVARYGDTTVLVTATMSEPREGIDYFPLMVNYEERVYAIGKIPGSITRREGR
PRDVATLAARLIDRPLRPLFPEGFRHDVQIIATVLSVDNDCEPDILALNGASVALTLSDIPFDGPIGGVKVGLVDGELVI
NPDEEEREKSKLDLTVAGTRDAVLMVEAGANEVSEDVMLDAIELAHQEIKRLVLLQEEIGEEAGKKKFEFTKDEITPELD
EEIRKYISSDMENALRIPEKLERNAKVDEIKENTLQYFEDMFENNGLDNEEKNKQLKMVERTIEKVMKEKVRKMIIEEGI
RPDGRKPDEIRPIWCEVGTLPRVHGSGVFTRGQTQALSVVTLGATSDEQILFGLGEEETKRYMHHYNFPPYSVGETSPLR
SPGRREIGHGALGERALQPVIPDQEEFPYTIRVVSEVLESNGSTSQASICGSTLALMDAGVPIKEPVAGIAMGLLKEDEK
VVILSDIQGLEDFYGDMDFKVAGTRNGITALQMDIKIHGISKEILKKALKRAREGRLYILDKMLQVIDKPRPELSPYAPL
MITMKVSPDKIRHIIGPGGKIINKIIDETGVEIDIDDDGSVYILAQDQESGNRAKEIINKLTKEVEVGDIYEGRVKKITN
FGAFVEILPGREGLVHISELADHHVKKVEDIVKIGDRIPVKVIEIDELGRINLSRKRALKEQKKE
>Mature_705_residues
MHKDWTIDVAGGKMKFETGKYAKQANGSVVARYGDTTVLVTATMSEPREGIDYFPLMVNYEERVYAIGKIPGSITRREGR
PRDVATLAARLIDRPLRPLFPEGFRHDVQIIATVLSVDNDCEPDILALNGASVALTLSDIPFDGPIGGVKVGLVDGELVI
NPDEEEREKSKLDLTVAGTRDAVLMVEAGANEVSEDVMLDAIELAHQEIKRLVLLQEEIGEEAGKKKFEFTKDEITPELD
EEIRKYISSDMENALRIPEKLERNAKVDEIKENTLQYFEDMFENNGLDNEEKNKQLKMVERTIEKVMKEKVRKMIIEEGI
RPDGRKPDEIRPIWCEVGTLPRVHGSGVFTRGQTQALSVVTLGATSDEQILFGLGEEETKRYMHHYNFPPYSVGETSPLR
SPGRREIGHGALGERALQPVIPDQEEFPYTIRVVSEVLESNGSTSQASICGSTLALMDAGVPIKEPVAGIAMGLLKEDEK
VVILSDIQGLEDFYGDMDFKVAGTRNGITALQMDIKIHGISKEILKKALKRAREGRLYILDKMLQVIDKPRPELSPYAPL
MITMKVSPDKIRHIIGPGGKIINKIIDETGVEIDIDDDGSVYILAQDQESGNRAKEIINKLTKEVEVGDIYEGRVKKITN
FGAFVEILPGREGLVHISELADHHVKKVEDIVKIGDRIPVKVIEIDELGRINLSRKRALKEQKKE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=722, Percent_Identity=37.3961218836565, Blast_Score=445, Evalue=1e-125,
Organism=Homo sapiens, GI9506689, Length=227, Percent_Identity=25.1101321585903, Blast_Score=67, Evalue=8e-11,
Organism=Escherichia coli, GI145693187, Length=691, Percent_Identity=50.2170767004341, Blast_Score=674, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=711, Percent_Identity=34.4585091420534, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI281362905, Length=717, Percent_Identity=38.4937238493724, Blast_Score=462, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24651641, Length=717, Percent_Identity=38.4937238493724, Blast_Score=462, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24651643, Length=717, Percent_Identity=38.4937238493724, Blast_Score=462, Evalue=1e-130,
Organism=Drosophila melanogaster, GI161079377, Length=668, Percent_Identity=38.0239520958084, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24584046, Length=227, Percent_Identity=26.8722466960352, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_HALOH (B8CW78)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002508542.1
- ProteinModelPortal:   B8CW78
- GeneID:   7314777
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_07900
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   CLSK2806302
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 78673; Mature: 78673

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKDWTIDVAGGKMKFETGKYAKQANGSVVARYGDTTVLVTATMSEPREGIDYFPLMVNY
CCCCEEEEECCCEEEECCCCHHHHCCCCEEEEECCEEEEEEEECCCCCCCCCCCCEEEEC
EERVYAIGKIPGSITRREGRPRDVATLAARLIDRPLRPLFPEGFRHDVQIIATVLSVDND
CCCEEEEECCCCCHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
CEPDILALNGASVALTLSDIPFDGPIGGVKVGLVDGELVINPDEEEREKSKLDLTVAGTR
CCCCEEEECCCEEEEEEECCCCCCCCCCEEEEEECCEEEECCCHHHHHHHHEEEEEECCC
DAVLMVEAGANEVSEDVMLDAIELAHQEIKRLVLLQEEIGEEAGKKKFEFTKDEITPELD
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHH
EEIRKYISSDMENALRIPEKLERNAKVDEIKENTLQYFEDMFENNGLDNEEKNKQLKMVE
HHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
RTIEKVMKEKVRKMIIEEGIRPDGRKPDEIRPIWCEVGTLPRVHGSGVFTRGQTQALSVV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCCEEEEE
TLGATSDEQILFGLGEEETKRYMHHYNFPPYSVGETSPLRSPGRREIGHGALGERALQPV
EECCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHHCCCC
IPDQEEFPYTIRVVSEVLESNGSTSQASICGSTLALMDAGVPIKEPVAGIAMGLLKEDEK
CCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
VVILSDIQGLEDFYGDMDFKVAGTRNGITALQMDIKIHGISKEILKKALKRAREGRLYIL
EEEEECCCHHHHHHCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCEEHH
DKMLQVIDKPRPELSPYAPLMITMKVSPDKIRHIIGPGGKIINKIIDETGVEIDIDDDGS
HHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCC
VYILAQDQESGNRAKEIINKLTKEVEVGDIYEGRVKKITNFGAFVEILPGREGLVHISEL
EEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCCCCEEHHHH
ADHHVKKVEDIVKIGDRIPVKVIEIDELGRINLSRKRALKEQKKE
HHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MHKDWTIDVAGGKMKFETGKYAKQANGSVVARYGDTTVLVTATMSEPREGIDYFPLMVNY
CCCCEEEEECCCEEEECCCCHHHHCCCCEEEEECCEEEEEEEECCCCCCCCCCCCEEEEC
EERVYAIGKIPGSITRREGRPRDVATLAARLIDRPLRPLFPEGFRHDVQIIATVLSVDND
CCCEEEEECCCCCHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
CEPDILALNGASVALTLSDIPFDGPIGGVKVGLVDGELVINPDEEEREKSKLDLTVAGTR
CCCCEEEECCCEEEEEEECCCCCCCCCCEEEEEECCEEEECCCHHHHHHHHEEEEEECCC
DAVLMVEAGANEVSEDVMLDAIELAHQEIKRLVLLQEEIGEEAGKKKFEFTKDEITPELD
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHH
EEIRKYISSDMENALRIPEKLERNAKVDEIKENTLQYFEDMFENNGLDNEEKNKQLKMVE
HHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
RTIEKVMKEKVRKMIIEEGIRPDGRKPDEIRPIWCEVGTLPRVHGSGVFTRGQTQALSVV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCCEEEEE
TLGATSDEQILFGLGEEETKRYMHHYNFPPYSVGETSPLRSPGRREIGHGALGERALQPV
EECCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHHCCCC
IPDQEEFPYTIRVVSEVLESNGSTSQASICGSTLALMDAGVPIKEPVAGIAMGLLKEDEK
CCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
VVILSDIQGLEDFYGDMDFKVAGTRNGITALQMDIKIHGISKEILKKALKRAREGRLYIL
EEEEECCCHHHHHHCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCEEHH
DKMLQVIDKPRPELSPYAPLMITMKVSPDKIRHIIGPGGKIINKIIDETGVEIDIDDDGS
HHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCC
VYILAQDQESGNRAKEIINKLTKEVEVGDIYEGRVKKITNFGAFVEILPGREGLVHISEL
EEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCCCCEEHHHH
ADHHVKKVEDIVKIGDRIPVKVIEIDELGRINLSRKRALKEQKKE
HHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA