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The map label for this gene is nfo

Identifier: 220931635

GI number: 220931635

Start: 850408

End: 851244

Strand: Direct

Name: nfo

Synonym: Hore_07910

Alternate gene names: 220931635

Gene position: 850408-851244 (Clockwise)

Preceding gene: 220931634

Following gene: 220931636

Centisome position: 32.99

GC content: 35.36

Gene sequence:

>837_bases
ATGAGATTAGGTAAGCATGTATCTATCGCTGGTGGGCTTTATAAAGCAACAGATAGAGCCACTAAAATAGGGTGTAATGC
CCTGCAAATTTTTGTAAAAAACCCACGTGGCTGGAAAATTAAAGAGGTTTCTGACTCAGAAATAAAAAAACTTAAAGACA
ATATTAAAAAAGAGAATATGTATCCCCTGGTGGTTCACTCTTCTTATTTAATTAACATGGCTACTCCCCGTGATGAGTTG
TGGGAGAAGTCTGTCAATTCCTTAAAGAAAGAATATAAGAGGACTGAATTAATAAATGCTGATTATTTTGTAGTACATCC
AGGTAGTCATACCGGAAAAGGCCTTCATTTTGGTATTAACAGGATAATCGAGGCTATAAACAGTGTTTTTGGAGAAGTTA
AAAATGGTCCCCAGCTATTGCTTGAAAATGTTGCTGGAGCCGGTTCAAGTATAGGCTCGAATTTTACTGAGTTACGTGAT
ATAATAAATAAAGTAGATGATTATGCCCGTATAGGGGTATGTCTTGATACCTGTCATGCCTTTGCAGCAGGTTATGACCT
AAGATATGAAGATGGTCTTGAAGAACTTTTAAATGATTTCGATAAGATTATCGGTCTTGATCTATTAAAGGTAATTCATT
TAAATGATTCCAAATATGGACTCGCTTCTAATAAAGATGAACATGCCCATATAGGGGAAGGAGAAATTGGTGAAAAAGGA
ATTTCAAATATAATTAACCATCCACTCTTAAAGGACAAACCCTTTATACTGGAAACACCAAAATTTAGTGGTAGAGATAA
AGATGTTGAGCTGGTTAATTTATTACGGAGGGATTAG

Upstream 100 bases:

>100_bases
AATATAAAGGGATTAAAAGGCATAAATTCATTAAACATAAACTAGGGCCCTAGTTTATGTTTTTTTATTATCACAAAATT
AGAGCAGGTGATAATTTATT

Downstream 100 bases:

>100_bases
TAACTAAAAGTGACTGTTGAAGAATTGTTTGCTAAATCAATAATAACAAATATTAGACATGACATTCAGGAAGCTGGAGG
ACAGGAAATATATTTGATCG

Product: apurinic endonuclease Apn1

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MRLGKHVSIAGGLYKATDRATKIGCNALQIFVKNPRGWKIKEVSDSEIKKLKDNIKKENMYPLVVHSSYLINMATPRDEL
WEKSVNSLKKEYKRTELINADYFVVHPGSHTGKGLHFGINRIIEAINSVFGEVKNGPQLLLENVAGAGSSIGSNFTELRD
IINKVDDYARIGVCLDTCHAFAAGYDLRYEDGLEELLNDFDKIIGLDLLKVIHLNDSKYGLASNKDEHAHIGEGEIGEKG
ISNIINHPLLKDKPFILETPKFSGRDKDVELVNLLRRD

Sequences:

>Translated_278_residues
MRLGKHVSIAGGLYKATDRATKIGCNALQIFVKNPRGWKIKEVSDSEIKKLKDNIKKENMYPLVVHSSYLINMATPRDEL
WEKSVNSLKKEYKRTELINADYFVVHPGSHTGKGLHFGINRIIEAINSVFGEVKNGPQLLLENVAGAGSSIGSNFTELRD
IINKVDDYARIGVCLDTCHAFAAGYDLRYEDGLEELLNDFDKIIGLDLLKVIHLNDSKYGLASNKDEHAHIGEGEIGEKG
ISNIINHPLLKDKPFILETPKFSGRDKDVELVNLLRRD
>Mature_278_residues
MRLGKHVSIAGGLYKATDRATKIGCNALQIFVKNPRGWKIKEVSDSEIKKLKDNIKKENMYPLVVHSSYLINMATPRDEL
WEKSVNSLKKEYKRTELINADYFVVHPGSHTGKGLHFGINRIIEAINSVFGEVKNGPQLLLENVAGAGSSIGSNFTELRD
IINKVDDYARIGVCLDTCHAFAAGYDLRYEDGLEELLNDFDKIIGLDLLKVIHLNDSKYGLASNKDEHAHIGEGEIGEKG
ISNIINHPLLKDKPFILETPKFSGRDKDVELVNLLRRD

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=260, Percent_Identity=38.4615384615385, Blast_Score=177, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI17531193, Length=279, Percent_Identity=39.0681003584229, Blast_Score=190, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6322735, Length=263, Percent_Identity=36.8821292775665, Blast_Score=183, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_HALOH (B8CW79)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002508543.1
- ProteinModelPortal:   B8CW79
- SMR:   B8CW79
- GeneID:   7314778
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_07910
- HOGENOM:   HBG565018
- OMA:   QIALETM
- ProtClustDB:   CLSK2806303
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 31126; Mature: 31126

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLGKHVSIAGGLYKATDRATKIGCNALQIFVKNPRGWKIKEVSDSEIKKLKDNIKKENM
CCCCCEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCEEECCCHHHHHHHHHHHHHCCC
YPLVVHSSYLINMATPRDELWEKSVNSLKKEYKRTELINADYFVVHPGSHTGKGLHFGIN
CEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHH
RIIEAINSVFGEVKNGPQLLLENVAGAGSSIGSNFTELRDIINKVDDYARIGVCLDTCHA
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FAAGYDLRYEDGLEELLNDFDKIIGLDLLKVIHLNDSKYGLASNKDEHAHIGEGEIGEKG
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCHHH
ISNIINHPLLKDKPFILETPKFSGRDKDVELVNLLRRD
HHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MRLGKHVSIAGGLYKATDRATKIGCNALQIFVKNPRGWKIKEVSDSEIKKLKDNIKKENM
CCCCCEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCEEECCCHHHHHHHHHHHHHCCC
YPLVVHSSYLINMATPRDELWEKSVNSLKKEYKRTELINADYFVVHPGSHTGKGLHFGIN
CEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHH
RIIEAINSVFGEVKNGPQLLLENVAGAGSSIGSNFTELRDIINKVDDYARIGVCLDTCHA
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FAAGYDLRYEDGLEELLNDFDKIIGLDLLKVIHLNDSKYGLASNKDEHAHIGEGEIGEKG
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCHHH
ISNIINHPLLKDKPFILETPKFSGRDKDVELVNLLRRD
HHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA