Click here to switch to the map view.

The map label for this gene is nusA [H]

Identifier: 220931626

GI number: 220931626

Start: 840765

End: 841898

Strand: Direct

Name: nusA [H]

Synonym: Hore_07820

Alternate gene names: 220931626

Gene position: 840765-841898 (Clockwise)

Preceding gene: 220931625

Following gene: 220931627

Centisome position: 32.61

GC content: 40.04

Gene sequence:

>1134_bases
ATGAATATTGAATTTTTACATGCGCTGGATGATATTGAAAAAGAAAAAGGAATATCCAAGGAAGTATTAATTGAAGCTAT
AGAAACAGCACTCCTTTCTGCCTATAAAAAAGAATTTGGTTCCAAGGAAAATGTTAGAGTACATATAGTTCCAGATAGTG
GTGAGGTACATGTTTATAGTCAGAAAAAAGTAGTTGATGAAGTTAATAATGAGCGAAAGGAAATTTCCCTGGAAGAGGCA
CAAAAATTAAACAAAAATTACGAACCAGGGGATATTGTGGAGAAGGAGGTAACTCCAGCTAATTTTGGTAGAATTGCTGC
CCAGACTGCAAAACAGGTTGTTATGCAGAGAATCAGAGAAGCTGAACGTGATGTAATTTATGAAGAATATAAACAAAAAG
AGGGGGAAATAATTACCGGTACTGTCCAGCGCTTTCATAATAACAACGTGCTTATTGACCTGGGGAGGACTGAAGCCCTG
TTACCGCCATCTGAACAAATCCCCGGGGAATTATATGAAATGGGTAGAAGGATTAAAGTATATGTTGTCGAAGTTGCTTC
AACTACAAAAGGGCCACGTATCCTCGTTTCCAGGTCCCATCCCGGCCTCCTTAAAAGATTATTTGAAGTTGAAGTACCGG
AGATATTTGATGGTATTGTTGAAATCAAGGCAGTGGCCCGGGAAGCAGGCTATAGATCAAAGATGGCAGTCAGTTCCTAT
GATGAGAATGTTGATCCGGTAGGTTCCTGTGTGGGGCCTAAAGGAATGAGGGTACAGGCGGTAGTTGACCAGCTTAACGG
TGAAAAGATTGATATTATTGAATGGAGTGAGGACCCTGCTATACTTGTAGCCAATGCCCTTAATCCGGCCAAGGTTATAA
ATGTTAGCCTTGATGAAGAAGAAAAGGTTGCTGAAGTAGTTGTACCTGATTTTCAATTATCTCTGGCTATTGGTAAAGAG
GGTCAGAATGCGAGATTAGCAGCCAAATTAACAGGATGGAAGGTTGATATCAAAAGTGAATCCCAGTTTGAGAAAGAAAC
CGGAGAGGTTGAGAAAAAAATTCCCGCAGATGATGGGGAAAAAGTTGATGAAGTAGGGGTTGAAGATGATAGTCTAGATA
TAGAAGAGAGTTAG

Upstream 100 bases:

>100_bases
AAATACCTTTTTCACAGATTGCTAAAGCCCATTTAATGGTTGATTTTGATAATATTACAGGTTAGATAATGTTGCAAGTT
TTTTATAAGGAGGAATTGGA

Downstream 100 bases:

>100_bases
GAGGGATTTATTATGTCCCGAAAGGTTCCGGTTCGAATGTGTATCGGGTGTCAGACCAGGAAGCCAAAAAAAGAGTTAAT
AAGAATAATATATAATAAAA

Product: NusA antitermination factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MNIEFLHALDDIEKEKGISKEVLIEAIETALLSAYKKEFGSKENVRVHIVPDSGEVHVYSQKKVVDEVNNERKEISLEEA
QKLNKNYEPGDIVEKEVTPANFGRIAAQTAKQVVMQRIREAERDVIYEEYKQKEGEIITGTVQRFHNNNVLIDLGRTEAL
LPPSEQIPGELYEMGRRIKVYVVEVASTTKGPRILVSRSHPGLLKRLFEVEVPEIFDGIVEIKAVAREAGYRSKMAVSSY
DENVDPVGSCVGPKGMRVQAVVDQLNGEKIDIIEWSEDPAILVANALNPAKVINVSLDEEEKVAEVVVPDFQLSLAIGKE
GQNARLAAKLTGWKVDIKSESQFEKETGEVEKKIPADDGEKVDEVGVEDDSLDIEES

Sequences:

>Translated_377_residues
MNIEFLHALDDIEKEKGISKEVLIEAIETALLSAYKKEFGSKENVRVHIVPDSGEVHVYSQKKVVDEVNNERKEISLEEA
QKLNKNYEPGDIVEKEVTPANFGRIAAQTAKQVVMQRIREAERDVIYEEYKQKEGEIITGTVQRFHNNNVLIDLGRTEAL
LPPSEQIPGELYEMGRRIKVYVVEVASTTKGPRILVSRSHPGLLKRLFEVEVPEIFDGIVEIKAVAREAGYRSKMAVSSY
DENVDPVGSCVGPKGMRVQAVVDQLNGEKIDIIEWSEDPAILVANALNPAKVINVSLDEEEKVAEVVVPDFQLSLAIGKE
GQNARLAAKLTGWKVDIKSESQFEKETGEVEKKIPADDGEKVDEVGVEDDSLDIEES
>Mature_377_residues
MNIEFLHALDDIEKEKGISKEVLIEAIETALLSAYKKEFGSKENVRVHIVPDSGEVHVYSQKKVVDEVNNERKEISLEEA
QKLNKNYEPGDIVEKEVTPANFGRIAAQTAKQVVMQRIREAERDVIYEEYKQKEGEIITGTVQRFHNNNVLIDLGRTEAL
LPPSEQIPGELYEMGRRIKVYVVEVASTTKGPRILVSRSHPGLLKRLFEVEVPEIFDGIVEIKAVAREAGYRSKMAVSSY
DENVDPVGSCVGPKGMRVQAVVDQLNGEKIDIIEWSEDPAILVANALNPAKVINVSLDEEEKVAEVVVPDFQLSLAIGKE
GQNARLAAKLTGWKVDIKSESQFEKETGEVEKKIPADDGEKVDEVGVEDDSLDIEES

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=340, Percent_Identity=40.8823529411765, Blast_Score=278, Evalue=4e-76,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 42033; Mature: 42033

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIEFLHALDDIEKEKGISKEVLIEAIETALLSAYKKEFGSKENVRVHIVPDSGEVHVYS
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEE
QKKVVDEVNNERKEISLEEAQKLNKNYEPGDIVEKEVTPANFGRIAAQTAKQVVMQRIRE
HHHHHHHHCCHHHHCCHHHHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHH
AERDVIYEEYKQKEGEIITGTVQRFHNNNVLIDLGRTEALLPPSEQIPGELYEMGRRIKV
HHHHHHHHHHHHCCCCEEEEEHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCEEEE
YVVEVASTTKGPRILVSRSHPGLLKRLFEVEVPEIFDGIVEIKAVAREAGYRSKMAVSSY
EEEEEECCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEHHCC
DENVDPVGSCVGPKGMRVQAVVDQLNGEKIDIIEWSEDPAILVANALNPAKVINVSLDEE
CCCCCCHHHCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCEEEEECCCCEEEEEEECCCH
EKVAEVVVPDFQLSLAIGKEGQNARLAAKLTGWKVDIKSESQFEKETGEVEKKIPADDGE
HHHHHHCCCCEEEEEEECCCCCCCEEEEEEECEEEEECCCHHHHHHHHHHHHCCCCCCCC
KVDEVGVEDDSLDIEES
CHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNIEFLHALDDIEKEKGISKEVLIEAIETALLSAYKKEFGSKENVRVHIVPDSGEVHVYS
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEE
QKKVVDEVNNERKEISLEEAQKLNKNYEPGDIVEKEVTPANFGRIAAQTAKQVVMQRIRE
HHHHHHHHCCHHHHCCHHHHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHH
AERDVIYEEYKQKEGEIITGTVQRFHNNNVLIDLGRTEALLPPSEQIPGELYEMGRRIKV
HHHHHHHHHHHHCCCCEEEEEHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCEEEE
YVVEVASTTKGPRILVSRSHPGLLKRLFEVEVPEIFDGIVEIKAVAREAGYRSKMAVSSY
EEEEEECCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEHHCC
DENVDPVGSCVGPKGMRVQAVVDQLNGEKIDIIEWSEDPAILVANALNPAKVINVSLDEE
CCCCCCHHHCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCEEEEECCCCEEEEEEECCCH
EKVAEVVVPDFQLSLAIGKEGQNARLAAKLTGWKVDIKSESQFEKETGEVEKKIPADDGE
HHHHHHCCCCEEEEEEECCCCCCCEEEEEEECEEEEECCCHHHHHHHHHHHHCCCCCCCC
KVDEVGVEDDSLDIEES
CHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]