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The map label for this gene is dacB [H]

Identifier: 220931566

GI number: 220931566

Start: 780503

End: 781636

Strand: Direct

Name: dacB [H]

Synonym: Hore_07220

Alternate gene names: 220931566

Gene position: 780503-781636 (Clockwise)

Preceding gene: 220931565

Following gene: 220931567

Centisome position: 30.27

GC content: 39.86

Gene sequence:

>1134_bases
ATGTTAAAAAAAACAGGAGTTTTGTTATTACTACTGGTTTTATTGTCTGTTAATATTTATAACACCAATTATGTAAAGGC
CAGTTCATTACCCAGGGTTGATGCTGCCGCTGCTGCCGTAGTTGATGTGGACACAGGTCAGGTTTTATACAATCACCGAA
TGAATCATAAATTGCCACCTGCCAGTACAACTAAAATCCTGACAACAATAATTGCTATTGAAGAGGGAGAACTAAATGAA
ATGGTAACCGTAAGTCGACGGGCAGCCTATCAGGAAGGTTCTTCGATATGGTTACAGGAAGGTGAGAAATTACCCTTAGA
AGATCTATTGTACGGGGTTATGCTGGCTTCAGGTAATGATGCTGCAGTTGCCGTTGCTGAACATATATCAGGTTCTGTAA
AAAAATTTTCTGAACTGATGAATAAACGGGCTAAAGAAATAGGGGCCACCAATAGCAATTTTTTAAATCCCAGTGGTCTA
CCTCAGTCCGGGCATTATTCAACTGCCCATGACCTGGCCATGATTATGAGGTATGCGTTAAAGAATGATATTTTTGCCCG
GATTACGTCAACAAAATATAAAACTATCTCCTGGCCAGGTAAAGAATGGGACCGGGGCCTTAGAAATCATAATAAACTTC
TCTGGAAATACCCTGATATTACTGGAGGTAAAACCGGTTATACCAGGGCTGCCGGTCGTTGTCTGGTAGCTTCTGCAAAG
CGGGATAACCGACATGTTGTAGCTGTTGTCTTAAACTGTCCCAATGACTGGGTGGAAATTAGAAAACTGCTTGACTATGG
CCTTAATAATTTTAAAAAAATAAGATATATAAAAAAAGGGCAGGTTTTACGGACCCTGGAGTGGACCAGGTCACGGGAGA
AAACTTTCAAGCTGGTTACCGGGGAAGATCTGGATATTGTTGTGCCTGAAGGGGGAAGTATAAAAATTAAAAAAGAAATA
GTTCTTAACCCTGATATTACTCTGCCCATTAATAAGGGTGATGTAGTTGGTGAACTCATTCTATACAGTGGGCAACGGAT
TATTGGAAAAGCAGATGTGATTTCTGGTAATGACCTGAATTTTAACTCTATTTTTTTGAGATTCTGGCATAAAATTAGTT
CCCGGGATAATTGA

Upstream 100 bases:

>100_bases
GTACCACTGCTGGTTCCATTATTCCTTTAGTTTAACCATGATAATAAAACCCATCTCCGGTGCATATAATTTTAAATAGT
AACCGGGAATGGGTGGGAGT

Downstream 100 bases:

>100_bases
AACGGGTACACTACAGGTTAGTCTTATATAGTCCAAACAGGTCAGGCCTGGCCATCTTGAAGTTAACATTAAATAGAGAT
ATAATAAATAATAGAGGTGC

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MLKKTGVLLLLLVLLSVNIYNTNYVKASSLPRVDAAAAAVVDVDTGQVLYNHRMNHKLPPASTTKILTTIIAIEEGELNE
MVTVSRRAAYQEGSSIWLQEGEKLPLEDLLYGVMLASGNDAAVAVAEHISGSVKKFSELMNKRAKEIGATNSNFLNPSGL
PQSGHYSTAHDLAMIMRYALKNDIFARITSTKYKTISWPGKEWDRGLRNHNKLLWKYPDITGGKTGYTRAAGRCLVASAK
RDNRHVVAVVLNCPNDWVEIRKLLDYGLNNFKKIRYIKKGQVLRTLEWTRSREKTFKLVTGEDLDIVVPEGGSIKIKKEI
VLNPDITLPINKGDVVGELILYSGQRIIGKADVISGNDLNFNSIFLRFWHKISSRDN

Sequences:

>Translated_377_residues
MLKKTGVLLLLLVLLSVNIYNTNYVKASSLPRVDAAAAAVVDVDTGQVLYNHRMNHKLPPASTTKILTTIIAIEEGELNE
MVTVSRRAAYQEGSSIWLQEGEKLPLEDLLYGVMLASGNDAAVAVAEHISGSVKKFSELMNKRAKEIGATNSNFLNPSGL
PQSGHYSTAHDLAMIMRYALKNDIFARITSTKYKTISWPGKEWDRGLRNHNKLLWKYPDITGGKTGYTRAAGRCLVASAK
RDNRHVVAVVLNCPNDWVEIRKLLDYGLNNFKKIRYIKKGQVLRTLEWTRSREKTFKLVTGEDLDIVVPEGGSIKIKKEI
VLNPDITLPINKGDVVGELILYSGQRIIGKADVISGNDLNFNSIFLRFWHKISSRDN
>Mature_377_residues
MLKKTGVLLLLLVLLSVNIYNTNYVKASSLPRVDAAAAAVVDVDTGQVLYNHRMNHKLPPASTTKILTTIIAIEEGELNE
MVTVSRRAAYQEGSSIWLQEGEKLPLEDLLYGVMLASGNDAAVAVAEHISGSVKKFSELMNKRAKEIGATNSNFLNPSGL
PQSGHYSTAHDLAMIMRYALKNDIFARITSTKYKTISWPGKEWDRGLRNHNKLLWKYPDITGGKTGYTRAAGRCLVASAK
RDNRHVVAVVLNCPNDWVEIRKLLDYGLNNFKKIRYIKKGQVLRTLEWTRSREKTFKLVTGEDLDIVVPEGGSIKIKKEI
VLNPDITLPINKGDVVGELILYSGQRIIGKADVISGNDLNFNSIFLRFWHKISSRDN

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=357, Percent_Identity=32.2128851540616, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI87082022, Length=383, Percent_Identity=30.0261096605744, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1786851, Length=329, Percent_Identity=30.0911854103343, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI87082054, Length=228, Percent_Identity=27.6315789473684, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42078; Mature: 42078

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKTGVLLLLLVLLSVNIYNTNYVKASSLPRVDAAAAAVVDVDTGQVLYNHRMNHKLPP
CCCCHHHHHHHHHHHHCEEECCCEEECCCCCCCCHHHEEEEECCCCCEEECCCCCCCCCC
ASTTKILTTIIAIEEGELNEMVTVSRRAAYQEGSSIWLQEGEKLPLEDLLYGVMLASGND
CCHHHHEEHEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHEEECCCC
AAVAVAEHISGSVKKFSELMNKRAKEIGATNSNFLNPSGLPQSGHYSTAHDLAMIMRYAL
CEEHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KNDIFARITSTKYKTISWPGKEWDRGLRNHNKLLWKYPDITGGKTGYTRAAGRCLVASAK
HCCCEEEEECCCEEEEECCCHHHHHHHHCCCEEEEECCCCCCCCCCCHHHCCEEEEEECC
RDNRHVVAVVLNCPNDWVEIRKLLDYGLNNFKKIRYIKKGQVLRTLEWTRSREKTFKLVT
CCCCEEEEEEEECCCHHHHHHHHHHHCCCCHHHHEEECCCCEEEHHHHHCCCCCEEEEEE
GEDLDIVVPEGGSIKIKKEIVLNPDITLPINKGDVVGELILYSGQRIIGKADVISGNDLN
CCCCEEEECCCCCEEEEEEEEECCCEEEEECCCCHHHHHHHHCCCEEECCEEEECCCCCC
FNSIFLRFWHKISSRDN
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLKKTGVLLLLLVLLSVNIYNTNYVKASSLPRVDAAAAAVVDVDTGQVLYNHRMNHKLPP
CCCCHHHHHHHHHHHHCEEECCCEEECCCCCCCCHHHEEEEECCCCCEEECCCCCCCCCC
ASTTKILTTIIAIEEGELNEMVTVSRRAAYQEGSSIWLQEGEKLPLEDLLYGVMLASGND
CCHHHHEEHEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHEEECCCC
AAVAVAEHISGSVKKFSELMNKRAKEIGATNSNFLNPSGLPQSGHYSTAHDLAMIMRYAL
CEEHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KNDIFARITSTKYKTISWPGKEWDRGLRNHNKLLWKYPDITGGKTGYTRAAGRCLVASAK
HCCCEEEEECCCEEEEECCCHHHHHHHHCCCEEEEECCCCCCCCCCCHHHCCEEEEEECC
RDNRHVVAVVLNCPNDWVEIRKLLDYGLNNFKKIRYIKKGQVLRTLEWTRSREKTFKLVT
CCCCEEEEEEEECCCHHHHHHHHHHHCCCCHHHHEEECCCCEEEHHHHHCCCCCEEEEEE
GEDLDIVVPEGGSIKIKKEIVLNPDITLPINKGDVVGELILYSGQRIIGKADVISGNDLN
CCCCEEEECCCCCEEEEEEEEECCCEEEEECCCCHHHHHHHHCCCEEECCEEEECCCCCC
FNSIFLRFWHKISSRDN
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]