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The map label for this gene is rluB [H]

Identifier: 220931567

GI number: 220931567

Start: 781748

End: 782461

Strand: Direct

Name: rluB [H]

Synonym: Hore_07230

Alternate gene names: 220931567

Gene position: 781748-782461 (Clockwise)

Preceding gene: 220931566

Following gene: 220931568

Centisome position: 30.32

GC content: 38.94

Gene sequence:

>714_bases
ATGGAAAGACTACAAAAATACATGGCCCATGCTGGGGTAGCTTCCCGTCGTAAGTCCGAAAAAATAATTACTCAGGGGAG
AGTAAAAGTTAATGGAAAAATTGTAACTGAACTTGGTACCAAGATAGATCCCGATAAGGATATTGTTGAGGTAGATGGTA
GGGTTATAAGCAAGGAAAAGTATGTATATATACTTTTAAATAAACCGGTCGGTTATATTACTTCAGTATCTGATCCCCAT
GGGAGAAAAACTGTTACCGGGCTGATTAAAGGTGTTTCCCAGCGTATTTACCCGGTGGGCAGGCTTGATTATGATACCAG
TGGGTTATTGATACTGACAAATGATGGTGAGTTAACATATGTTTTAACTCATCCTTCCCATATGGTTGAAAAAACATATC
GGGTTGAGGTCAAAGGTTTTCCTTCTGATGAAGTTTTGAAGAAACTGGAGACAGGGGTCAAATTAAAAGATGGTCTTACT
GCTCCGGCCCGGGTGTCGGCAGTAAAAAGGAAAGAGAAGAACACAATTTTTAACCTGACCATCCATGAGGGTAGAAACAG
GCAGGTAAGAAGAATGTGTGATAAAATTGGATTTCCTGTAGTTAACTTAAAAAGAATTGCCTTTGGTCCCCTCAATCTCA
AAGGGCTTAAAACCGGACAGTACAGATTTCTCACTGAAAAAGAAGTTAAAGCCTTAAAGAACATAAAAGAATAA

Upstream 100 bases:

>100_bases
TACAGGTTAGTCTTATATAGTCCAAACAGGTCAGGCCTGGCCATCTTGAAGTTAACATTAAATAGAGATATAATAAATAA
TAGAGGTGCAAATACATATT

Downstream 100 bases:

>100_bases
AATTAAAATGAAGGAATTTTTTAATAAATGACGAATAAATAAAATAGCCCTACATAATTTTAGTAAATAAAGTAATATTT
TAAAGTAATAATTAAAAATT

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MERLQKYMAHAGVASRRKSEKIITQGRVKVNGKIVTELGTKIDPDKDIVEVDGRVISKEKYVYILLNKPVGYITSVSDPH
GRKTVTGLIKGVSQRIYPVGRLDYDTSGLLILTNDGELTYVLTHPSHMVEKTYRVEVKGFPSDEVLKKLETGVKLKDGLT
APARVSAVKRKEKNTIFNLTIHEGRNRQVRRMCDKIGFPVVNLKRIAFGPLNLKGLKTGQYRFLTEKEVKALKNIKE

Sequences:

>Translated_237_residues
MERLQKYMAHAGVASRRKSEKIITQGRVKVNGKIVTELGTKIDPDKDIVEVDGRVISKEKYVYILLNKPVGYITSVSDPH
GRKTVTGLIKGVSQRIYPVGRLDYDTSGLLILTNDGELTYVLTHPSHMVEKTYRVEVKGFPSDEVLKKLETGVKLKDGLT
APARVSAVKRKEKNTIFNLTIHEGRNRQVRRMCDKIGFPVVNLKRIAFGPLNLKGLKTGQYRFLTEKEVKALKNIKE
>Mature_237_residues
MERLQKYMAHAGVASRRKSEKIITQGRVKVNGKIVTELGTKIDPDKDIVEVDGRVISKEKYVYILLNKPVGYITSVSDPH
GRKTVTGLIKGVSQRIYPVGRLDYDTSGLLILTNDGELTYVLTHPSHMVEKTYRVEVKGFPSDEVLKKLETGVKLKDGLT
APARVSAVKRKEKNTIFNLTIHEGRNRQVRRMCDKIGFPVVNLKRIAFGPLNLKGLKTGQYRFLTEKEVKALKNIKE

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=244, Percent_Identity=39.7540983606557, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1790453, Length=230, Percent_Identity=34.7826086956522, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=35.8974358974359, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI87081838, Length=178, Percent_Identity=39.8876404494382, Blast_Score=122, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 26731; Mature: 26731

Theoretical pI: Translated: 10.59; Mature: 10.59

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLQKYMAHAGVASRRKSEKIITQGRVKVNGKIVTELGTKIDPDKDIVEVDGRVISKEK
CHHHHHHHHHCCHHHHHHHHHHHHCCEEEECCEEEEECCCCCCCCCCEEEECCEEECCCC
YVYILLNKPVGYITSVSDPHGRKTVTGLIKGVSQRIYPVGRLDYDTSGLLILTNDGELTY
EEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEE
VLTHPSHMVEKTYRVEVKGFPSDEVLKKLETGVKLKDGLTAPARVSAVKRKEKNTIFNLT
EEECCHHHHCCEEEEEEECCCHHHHHHHHHCCCEECCCCCCCHHHHHHHHHCCCEEEEEE
IHEGRNRQVRRMCDKIGFPVVNLKRIAFGPLNLKGLKTGQYRFLTEKEVKALKNIKE
EECCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCEEEECHHHHHHHHHCCC
>Mature Secondary Structure
MERLQKYMAHAGVASRRKSEKIITQGRVKVNGKIVTELGTKIDPDKDIVEVDGRVISKEK
CHHHHHHHHHCCHHHHHHHHHHHHCCEEEECCEEEEECCCCCCCCCCEEEECCEEECCCC
YVYILLNKPVGYITSVSDPHGRKTVTGLIKGVSQRIYPVGRLDYDTSGLLILTNDGELTY
EEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEE
VLTHPSHMVEKTYRVEVKGFPSDEVLKKLETGVKLKDGLTAPARVSAVKRKEKNTIFNLT
EEECCHHHHCCEEEEEEECCCHHHHHHHHHCCCEECCCCCCCHHHHHHHHHCCCEEEEEE
IHEGRNRQVRRMCDKIGFPVVNLKRIAFGPLNLKGLKTGQYRFLTEKEVKALKNIKE
EECCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCEEEECHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]