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The map label for this gene is dacF [H]

Identifier: 220931542

GI number: 220931542

Start: 757580

End: 758773

Strand: Direct

Name: dacF [H]

Synonym: Hore_06980

Alternate gene names: 220931542

Gene position: 757580-758773 (Clockwise)

Preceding gene: 220931541

Following gene: 220931543

Centisome position: 29.38

GC content: 38.44

Gene sequence:

>1194_bases
ATGTTTTGCAGGGTTAATAAAGGAAAATTTATTAATATAATCTTATTCTTTTTTTTATTTATTCTGGTATTCCCCTTTTA
TACCATGGCAGAACAACCGTCTTTTGATTTAACAGCAAAATCAGCCCTATTGATGCAGGTTAATACAGGTGAAATTCTTT
ATGAGAAGAATCCTCATCAAAAATTACCACCGGCAAGTATGACAAAAATTATGACAATGTTATTAGTAATGGAAGCCCTT
GAAGATGGAAGAGCCAGCTTGAGAGATTCCATCATAGTCAGTGAGAGGGCAGCCAGTATGGGTGGGTCACAGGTTTACCT
GGAACCGGGTGAAGAGATGACTCTTGAAGACCTGATGAAGGCTATAGCCATAGCCTCTGCCAATGATGCCTGTGTTGCCG
TTGCTGAATACCTGTACGGTACTGAAGAGGACTTTGTTAAAAAAATGAATGAAAAAGCCCGGGAACTGGGATGTAAAAAT
ACATACTTTTATAATACAAATGGTCTCCCCCCTGGTGACCCCTCTGTAGAAGGAAACTATACTTCAGCTTACGATCTGGC
GATTATGTCCCGGGAGTTATTAAAATATCCAGAGGTACTTAAATGGACGTCAACCTGGATAGATTACCTCCGGGATGGGG
AATTTGTCTTAAATAACACCAACAGGCTGGTTAGACATTATAAAGGTGTTGATGGTATAAAAACCGGGTATACGGAAGAG
GCTAAATTCTGTGTAGCGACTACTGCGGCCAGGAATGGTATCAGGTTTATTGCTGTTATTATGGGGGCTGATACTTCAAA
GGCAAGATTTAAAGAGGCTGCCCAGCTTCTTTCTTATGGGTTTAGTAAATACAGGAGCTTTATCGTAGCCGATAAAGAAG
AAGTAATAGATGAAATAACTATTCAAAACGCCAAGAATGAACAGGCCCGGGTTTTAACCAGAAATAAAGTAGTAATCCCT
GTAAAAAAGGGAGAAGAGGATGAAATAACGAAAAAAATTATAATAAAAGAAAACATAAAGGCCCCCCTGAAAAAGGGAGA
TTCAGTAGGTAAAATCAGGATATATAAAGGTAATTTATTATTAAAGGAAGCGGATCTTGTCATTGATAGAGATGTAACCA
GGGCTTCGTTGTGGCAAATGATAACTAGAATAATCAAAATGATGGTAACCAGTCTGGTTAAACTTTTTACTTGA

Upstream 100 bases:

>100_bases
TAATCGAATAATTACTTTAAAACAATACCTGTCCCTCCTGCATAAACCCCGAGATTAGGTAATAAATATCTACTATAAGG
GTAAAGGAGGGACTATATAT

Downstream 100 bases:

>100_bases
AGAAGGAAAACCCTTTTTATCATAGAATATAGATATAATTGATAAATATCTAACTAACGCTGTTGTAAAGAGGTGGTAAA
GATGACAGTATTTAATAGAA

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MFCRVNKGKFINIILFFFLFILVFPFYTMAEQPSFDLTAKSALLMQVNTGEILYEKNPHQKLPPASMTKIMTMLLVMEAL
EDGRASLRDSIIVSERAASMGGSQVYLEPGEEMTLEDLMKAIAIASANDACVAVAEYLYGTEEDFVKKMNEKARELGCKN
TYFYNTNGLPPGDPSVEGNYTSAYDLAIMSRELLKYPEVLKWTSTWIDYLRDGEFVLNNTNRLVRHYKGVDGIKTGYTEE
AKFCVATTAARNGIRFIAVIMGADTSKARFKEAAQLLSYGFSKYRSFIVADKEEVIDEITIQNAKNEQARVLTRNKVVIP
VKKGEEDEITKKIIIKENIKAPLKKGDSVGKIRIYKGNLLLKEADLVIDRDVTRASLWQMITRIIKMMVTSLVKLFT

Sequences:

>Translated_397_residues
MFCRVNKGKFINIILFFFLFILVFPFYTMAEQPSFDLTAKSALLMQVNTGEILYEKNPHQKLPPASMTKIMTMLLVMEAL
EDGRASLRDSIIVSERAASMGGSQVYLEPGEEMTLEDLMKAIAIASANDACVAVAEYLYGTEEDFVKKMNEKARELGCKN
TYFYNTNGLPPGDPSVEGNYTSAYDLAIMSRELLKYPEVLKWTSTWIDYLRDGEFVLNNTNRLVRHYKGVDGIKTGYTEE
AKFCVATTAARNGIRFIAVIMGADTSKARFKEAAQLLSYGFSKYRSFIVADKEEVIDEITIQNAKNEQARVLTRNKVVIP
VKKGEEDEITKKIIIKENIKAPLKKGDSVGKIRIYKGNLLLKEADLVIDRDVTRASLWQMITRIIKMMVTSLVKLFT
>Mature_397_residues
MFCRVNKGKFINIILFFFLFILVFPFYTMAEQPSFDLTAKSALLMQVNTGEILYEKNPHQKLPPASMTKIMTMLLVMEAL
EDGRASLRDSIIVSERAASMGGSQVYLEPGEEMTLEDLMKAIAIASANDACVAVAEYLYGTEEDFVKKMNEKARELGCKN
TYFYNTNGLPPGDPSVEGNYTSAYDLAIMSRELLKYPEVLKWTSTWIDYLRDGEFVLNNTNRLVRHYKGVDGIKTGYTEE
AKFCVATTAARNGIRFIAVIMGADTSKARFKEAAQLLSYGFSKYRSFIVADKEEVIDEITIQNAKNEQARVLTRNKVVIP
VKKGEEDEITKKIIIKENIKAPLKKGDSVGKIRIYKGNLLLKEADLVIDRDVTRASLWQMITRIIKMMVTSLVKLFT

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=391, Percent_Identity=31.2020460358056, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI87082022, Length=370, Percent_Identity=30.8108108108108, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1786851, Length=372, Percent_Identity=28.2258064516129, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI87082054, Length=283, Percent_Identity=27.5618374558304, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 44866; Mature: 44866

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFCRVNKGKFINIILFFFLFILVFPFYTMAEQPSFDLTAKSALLMQVNTGEILYEKNPHQ
CEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCEEEEEECCCCEEEECCCCC
KLPPASMTKIMTMLLVMEALEDGRASLRDSIIVSERAASMGGSQVYLEPGEEMTLEDLMK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHH
AIAIASANDACVAVAEYLYGTEEDFVKKMNEKARELGCKNTYFYNTNGLPPGDPSVEGNY
HHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCC
TSAYDLAIMSRELLKYPEVLKWTSTWIDYLRDGEFVLNNTNRLVRHYKGVDGIKTGYTEE
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHCCCCCCCCCCCCC
AKFCVATTAARNGIRFIAVIMGADTSKARFKEAAQLLSYGFSKYRSFIVADKEEVIDEIT
CCEEEEEHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IQNAKNEQARVLTRNKVVIPVKKGEEDEITKKIIIKENIKAPLKKGDSVGKIRIYKGNLL
HCCCCCCHHHEEECCEEEEEECCCCHHHHHHHHHHHHCCCCHHHCCCCCCEEEEEECCEE
LKEADLVIDRDVTRASLWQMITRIIKMMVTSLVKLFT
EEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFCRVNKGKFINIILFFFLFILVFPFYTMAEQPSFDLTAKSALLMQVNTGEILYEKNPHQ
CEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCEEEEEECCCCEEEECCCCC
KLPPASMTKIMTMLLVMEALEDGRASLRDSIIVSERAASMGGSQVYLEPGEEMTLEDLMK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHH
AIAIASANDACVAVAEYLYGTEEDFVKKMNEKARELGCKNTYFYNTNGLPPGDPSVEGNY
HHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCC
TSAYDLAIMSRELLKYPEVLKWTSTWIDYLRDGEFVLNNTNRLVRHYKGVDGIKTGYTEE
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHCCCCCCCCCCCCC
AKFCVATTAARNGIRFIAVIMGADTSKARFKEAAQLLSYGFSKYRSFIVADKEEVIDEIT
CCEEEEEHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IQNAKNEQARVLTRNKVVIPVKKGEEDEITKKIIIKENIKAPLKKGDSVGKIRIYKGNLL
HCCCCCCHHHEEECCEEEEEECCCCHHHHHHHHHHHHCCCCHHHCCCCCCEEEEEECCEE
LKEADLVIDRDVTRASLWQMITRIIKMMVTSLVKLFT
EEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]