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The map label for this gene is glgB

Identifier: 220931537

GI number: 220931537

Start: 749779

End: 751671

Strand: Reverse

Name: glgB

Synonym: Hore_06930

Alternate gene names: 220931537

Gene position: 751671-749779 (Counterclockwise)

Preceding gene: 220931556

Following gene: 220931536

Centisome position: 29.16

GC content: 37.88

Gene sequence:

>1893_bases
TTGAAAGATAAAATACCGACTGATTATGACCGATATTTATTTCACCAGGGAAGCCACTATAAAAGCTATGAATTTTTAGG
AGCTCACCCCTGCGAAGAAAATGGAAAAACAGGGGTTCGTTTTTCAGTCTGGGCCCCTAACGCTGCCGAAGTCAGGGTTA
TTGGCAGTTTCAATAATTGGGATGGTAATATAAATCCCATGGAAAGAATTAATGATTCTGGAATATGGACTACCTTCATA
CCTGAGGCCAAAAAGGGAGATCTTTATAAATATGAAATATTAACAAAATATGGAGATACCAGAATAAAGTCTGATCCTTA
TGCCTTTTATGCTGAACGCAAACCAAAAACTGCCTCAATTGTTTACTCCCTTAATAATTACAAGTGGAATGATAAAAAAT
GGATGGACAAGAAAAAAGATTATACAGTGTATAATAAACCGGTCTTAATATATGAAGTCCACCTCGGGTCCTGGAAACGT
AAGGAAAATGGGGATTATCTAACCTACCGGGAACTGGCAAATGAGCTTGTCGAATATGTCAAAGATATGGGGTATAACTA
CATTGAATTGTTACCGGTTGCAGAACACCCCTTTGACGGTTCCTGGGGTTATCAGTTAACCAACTACTATTCTGTTACGA
GTCGTTATGGGACACCTGAAGATTTTATGTATTTTATCGACAAATGTCACCAGAATGGTATCGGGGTTATCCTGGACTGG
GTACCCGGGCATTTCTGCAAAGATGACCATGGACTAAGGTTATTTGATGGAACTGCCCTTTATGAACCCCAGGATCCCCG
GAAGGCTGAAAATGAATGGGATACTTTAAACTTTGATTTTAACCAGCCAGAAGTATGGAGTTTCCTTATCTCTAATGCAG
TTTTCTGGTTTGATATCTACCATATCGACGGACTCAGGGTTGATGCTGTAAGTAACCTTTTATATTTAAACCATGGAAAA
GGTGACGGGGAATGGGTCCCCAACAAATATGGTGGCCATGAAAACCTGGAAGCTATTGATTTTATAAAAACCCTTAATAA
AGTAATTTTTGAGTATTTCCCCAATCCCCTGATGATTGCTGAAGAATCCAGCGCCTGGCCCCTGGTAACTTACCCAGCTC
ATCTTGGTGGTCTCGGTTTTAATTATAAGTGGAATATGGGATGGATGAATGATACCCTGGAATACATGGAAATGGATTCT
ATATATCGTAAATACCATCATAACCTCCTGACCTTCTCTTTTATGTACATGTTTTCAGAAAACTTTGTTTTACCTCTATC
CCATGATGAAGTAGTTCATGGAAAAAAATCTTTACTTGATAAAATGCCCGGTGATTACTGGCAGAAATTTGCTAATTTAA
GAACCCTTTATGGATATATGATGGGACACCCCGGTAAAAAATTATTATTTATGGGTGGAGAATTTGGACAATTTATCGAG
TGGAACTATAAACAGGGACTTGACTGGCTATTACTCGATTATGATATGCATAAAAAACTCCAGAATTATGTCAGGGAATT
AAACTACTTCTATAGACAGGAAAAAACCCTGTGGGAAGGGGATCATGAAGATGGGGGATTTGAATGGATTGATCCCCATG
ACAGTGAACAGAGTATCATTACATTTATGAGAAAAAACAAAGATGGAAGTGACTATACTATTATTGTCTGTAATTTTACT
CCTGAAGTCAGACATAATTACAGAATTGGTGTTCCTGAGTTTAAAGAATATAAGGAGGTATTTAATAGTGACCTGGAAAA
ATTCGGTGGGTCCGGCCAGAAAAATTCTACAATTATACAGCCAAGTGAACAACCATGGCATAACAGACCATATTCAATAG
AAATAACAATACCTCCTCTGGCAACAATATTTTTTAAACCCCTGAATCAATAA

Upstream 100 bases:

>100_bases
GGAATTATTTATTAAAGAGGAAAATTTTTATTATTTTTTAATACAGGGATGAATATACATTTAATATAACCCATTTTACA
AAAGAAAGGATGGTGAAAAA

Downstream 100 bases:

>100_bases
AAAGAAATAGTTTAAACTTTATTACTTTTAATAAAACTAAATAGCCGGTTATTTTTATATAAAAATAAATATATAAATTT
TTATTGGGTTTAAATTTAAA

Product: 1,4-alpha-glucan branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 630; Mature: 630

Protein sequence:

>630_residues
MKDKIPTDYDRYLFHQGSHYKSYEFLGAHPCEENGKTGVRFSVWAPNAAEVRVIGSFNNWDGNINPMERINDSGIWTTFI
PEAKKGDLYKYEILTKYGDTRIKSDPYAFYAERKPKTASIVYSLNNYKWNDKKWMDKKKDYTVYNKPVLIYEVHLGSWKR
KENGDYLTYRELANELVEYVKDMGYNYIELLPVAEHPFDGSWGYQLTNYYSVTSRYGTPEDFMYFIDKCHQNGIGVILDW
VPGHFCKDDHGLRLFDGTALYEPQDPRKAENEWDTLNFDFNQPEVWSFLISNAVFWFDIYHIDGLRVDAVSNLLYLNHGK
GDGEWVPNKYGGHENLEAIDFIKTLNKVIFEYFPNPLMIAEESSAWPLVTYPAHLGGLGFNYKWNMGWMNDTLEYMEMDS
IYRKYHHNLLTFSFMYMFSENFVLPLSHDEVVHGKKSLLDKMPGDYWQKFANLRTLYGYMMGHPGKKLLFMGGEFGQFIE
WNYKQGLDWLLLDYDMHKKLQNYVRELNYFYRQEKTLWEGDHEDGGFEWIDPHDSEQSIITFMRKNKDGSDYTIIVCNFT
PEVRHNYRIGVPEFKEYKEVFNSDLEKFGGSGQKNSTIIQPSEQPWHNRPYSIEITIPPLATIFFKPLNQ

Sequences:

>Translated_630_residues
MKDKIPTDYDRYLFHQGSHYKSYEFLGAHPCEENGKTGVRFSVWAPNAAEVRVIGSFNNWDGNINPMERINDSGIWTTFI
PEAKKGDLYKYEILTKYGDTRIKSDPYAFYAERKPKTASIVYSLNNYKWNDKKWMDKKKDYTVYNKPVLIYEVHLGSWKR
KENGDYLTYRELANELVEYVKDMGYNYIELLPVAEHPFDGSWGYQLTNYYSVTSRYGTPEDFMYFIDKCHQNGIGVILDW
VPGHFCKDDHGLRLFDGTALYEPQDPRKAENEWDTLNFDFNQPEVWSFLISNAVFWFDIYHIDGLRVDAVSNLLYLNHGK
GDGEWVPNKYGGHENLEAIDFIKTLNKVIFEYFPNPLMIAEESSAWPLVTYPAHLGGLGFNYKWNMGWMNDTLEYMEMDS
IYRKYHHNLLTFSFMYMFSENFVLPLSHDEVVHGKKSLLDKMPGDYWQKFANLRTLYGYMMGHPGKKLLFMGGEFGQFIE
WNYKQGLDWLLLDYDMHKKLQNYVRELNYFYRQEKTLWEGDHEDGGFEWIDPHDSEQSIITFMRKNKDGSDYTIIVCNFT
PEVRHNYRIGVPEFKEYKEVFNSDLEKFGGSGQKNSTIIQPSEQPWHNRPYSIEITIPPLATIFFKPLNQ
>Mature_630_residues
MKDKIPTDYDRYLFHQGSHYKSYEFLGAHPCEENGKTGVRFSVWAPNAAEVRVIGSFNNWDGNINPMERINDSGIWTTFI
PEAKKGDLYKYEILTKYGDTRIKSDPYAFYAERKPKTASIVYSLNNYKWNDKKWMDKKKDYTVYNKPVLIYEVHLGSWKR
KENGDYLTYRELANELVEYVKDMGYNYIELLPVAEHPFDGSWGYQLTNYYSVTSRYGTPEDFMYFIDKCHQNGIGVILDW
VPGHFCKDDHGLRLFDGTALYEPQDPRKAENEWDTLNFDFNQPEVWSFLISNAVFWFDIYHIDGLRVDAVSNLLYLNHGK
GDGEWVPNKYGGHENLEAIDFIKTLNKVIFEYFPNPLMIAEESSAWPLVTYPAHLGGLGFNYKWNMGWMNDTLEYMEMDS
IYRKYHHNLLTFSFMYMFSENFVLPLSHDEVVHGKKSLLDKMPGDYWQKFANLRTLYGYMMGHPGKKLLFMGGEFGQFIE
WNYKQGLDWLLLDYDMHKKLQNYVRELNYFYRQEKTLWEGDHEDGGFEWIDPHDSEQSIITFMRKNKDGSDYTIIVCNFT
PEVRHNYRIGVPEFKEYKEVFNSDLEKFGGSGQKNSTIIQPSEQPWHNRPYSIEITIPPLATIFFKPLNQ

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=663, Percent_Identity=27.6018099547511, Blast_Score=206, Evalue=4e-53,
Organism=Escherichia coli, GI1789839, Length=622, Percent_Identity=50, Blast_Score=642, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554896, Length=666, Percent_Identity=27.1771771771772, Blast_Score=207, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI32564391, Length=613, Percent_Identity=26.7536704730832, Blast_Score=187, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6320826, Length=671, Percent_Identity=26.3785394932936, Blast_Score=192, Evalue=9e-50,
Organism=Drosophila melanogaster, GI28573410, Length=646, Percent_Identity=27.8637770897833, Blast_Score=197, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_HALOH (B8CVY1)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002508445.1
- ProteinModelPortal:   B8CVY1
- GeneID:   7312928
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_06930
- HOGENOM:   HBG287139
- OMA:   DGTCLYE
- ProtClustDB:   CLSK2479208
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat

EC number: =2.4.1.18

Molecular weight: Translated: 74402; Mature: 74402

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: ACT_SITE 204-204 ACT_SITE 239-239 ACT_SITE 244-244 ACT_SITE 306-306 ACT_SITE 308-308 ACT_SITE 361-361 ACT_SITE 428-428 ACT_SITE 429-429

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDKIPTDYDRYLFHQGSHYKSYEFLGAHPCEENGKTGVRFSVWAPNAAEVRVIGSFNNW
CCCCCCCCHHHHHHCCCCCCCCEEECCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCC
DGNINPMERINDSGIWTTFIPEAKKGDLYKYEILTKYGDTRIKSDPYAFYAERKPKTASI
CCCCCHHHHCCCCCCEEEECCCCCCCCEEEEEEEECCCCCCCCCCCEEEEECCCCCEEEE
VYSLNNYKWNDKKWMDKKKDYTVYNKPVLIYEVHLGSWKRKENGDYLTYRELANELVEYV
EEEECCCEECCHHHCCCCCCCEEECCCEEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHH
KDMGYNYIELLPVAEHPFDGSWGYQLTNYYSVTSRYGTPEDFMYFIDKCHQNGIGVILDW
HHCCCCEEEEEECCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEC
VPGHFCKDDHGLRLFDGTALYEPQDPRKAENEWDTLNFDFNQPEVWSFLISNAVFWFDIY
CCCCCCCCCCCEEEECCEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHCEEEEEEEE
HIDGLRVDAVSNLLYLNHGKGDGEWVPNKYGGHENLEAIDFIKTLNKVIFEYFPNPLMIA
EECCEEEHHHCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
EESSAWPLVTYPAHLGGLGFNYKWNMGWMNDTLEYMEMDSIYRKYHHNLLTFSFMYMFSE
ECCCCCCEEECCHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NFVLPLSHDEVVHGKKSLLDKMPGDYWQKFANLRTLYGYMMGHPGKKLLFMGGEFGQFIE
CCEEECCCCHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEE
WNYKQGLDWLLLDYDMHKKLQNYVRELNYFYRQEKTLWEGDHEDGGFEWIDPHDSEQSII
EEHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCEEECCCCCCHHHHH
TFMRKNKDGSDYTIIVCNFTPEVRHNYRIGVPEFKEYKEVFNSDLEKFGGSGQKNSTIIQ
HHHHCCCCCCCEEEEEEECCHHHCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEC
PSEQPWHNRPYSIEITIPPLATIFFKPLNQ
CCCCCCCCCCEEEEEECCCCEEHHHCCCCC
>Mature Secondary Structure
MKDKIPTDYDRYLFHQGSHYKSYEFLGAHPCEENGKTGVRFSVWAPNAAEVRVIGSFNNW
CCCCCCCCHHHHHHCCCCCCCCEEECCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCC
DGNINPMERINDSGIWTTFIPEAKKGDLYKYEILTKYGDTRIKSDPYAFYAERKPKTASI
CCCCCHHHHCCCCCCEEEECCCCCCCCEEEEEEEECCCCCCCCCCCEEEEECCCCCEEEE
VYSLNNYKWNDKKWMDKKKDYTVYNKPVLIYEVHLGSWKRKENGDYLTYRELANELVEYV
EEEECCCEECCHHHCCCCCCCEEECCCEEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHH
KDMGYNYIELLPVAEHPFDGSWGYQLTNYYSVTSRYGTPEDFMYFIDKCHQNGIGVILDW
HHCCCCEEEEEECCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEC
VPGHFCKDDHGLRLFDGTALYEPQDPRKAENEWDTLNFDFNQPEVWSFLISNAVFWFDIY
CCCCCCCCCCCEEEECCEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHCEEEEEEEE
HIDGLRVDAVSNLLYLNHGKGDGEWVPNKYGGHENLEAIDFIKTLNKVIFEYFPNPLMIA
EECCEEEHHHCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
EESSAWPLVTYPAHLGGLGFNYKWNMGWMNDTLEYMEMDSIYRKYHHNLLTFSFMYMFSE
ECCCCCCEEECCHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NFVLPLSHDEVVHGKKSLLDKMPGDYWQKFANLRTLYGYMMGHPGKKLLFMGGEFGQFIE
CCEEECCCCHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEE
WNYKQGLDWLLLDYDMHKKLQNYVRELNYFYRQEKTLWEGDHEDGGFEWIDPHDSEQSII
EEHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCEEECCCCCCHHHHH
TFMRKNKDGSDYTIIVCNFTPEVRHNYRIGVPEFKEYKEVFNSDLEKFGGSGQKNSTIIQ
HHHHCCCCCCCEEEEEEECCHHHCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEC
PSEQPWHNRPYSIEITIPPLATIFFKPLNQ
CCCCCCCCCCEEEEEECCCCEEHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA