Click here to switch to the map view.

The map label for this gene is nuoG [C]

Identifier: 220931477

GI number: 220931477

Start: 682455

End: 684191

Strand: Direct

Name: nuoG [C]

Synonym: Hore_06330

Alternate gene names: 220931477

Gene position: 682455-684191 (Clockwise)

Preceding gene: 220931476

Following gene: 220931480

Centisome position: 26.47

GC content: 42.2

Gene sequence:

>1737_bases
ATGTCTGATAAAGTAAATATAACATTAGATGGTAAAAGTTTAACTGTAGATAAAGATAAAACTATTCTGGAAGTAGCCCG
GGAAGCCGGGATTAAGATTCCTACCCTCTGTTACCTGGAAGAAATTAATGAAATTGGTAGCTGCAGGGTCTGTGTAGTGG
AAGTGAATGGAAAAATACAGCCTGCCTGCGTTACTCCGGTAAGTGAAGGCCTGGAAATCACAACAACTTCACCCAGGATT
CGTGAAGCCAGGAGGATATCCCTCGAGTTAATAATTTCAGACCATCCTATGGAATGTTTGACCTGTAGTCGGAATGGAAA
TTGTGAACTCCAGAGACTGGCAGAGGACTTTGGAATAAGTGAGATAACTTATGAGGGTGAACAGTCACATTTTGAACCTG
ATCTTTCATCACCTTCAATTGTCAGGGATCCCGATAAATGTATTTTATGCCGGCGTTGTGTTAGTGTCTGTGAACAGGTT
CAGGGGGTTGCTGCCTTAACTCCCAATGAAAGGGGATTTTCTACCATAATTACCCCTGCCTTTGGTCAAAAACTGGGTGA
AATAGCATGTGCTAACTGTGGTCAGTGTATAAATGCGTGTCCAGTTGGAGCCCTTTCTGAAAAGGATGACACCGAAAAGG
TCTGGGAAGCCCTGGCTAATCCCGATAAACATGTAGTGGTCCAGACAGCACCTGCTGTCAGGGTATCGATTGGTGAAGTA
TTTGGAATGAAACCCGGTAGTCTGGTAACAGGAAAACTGATGGCTGGTTTAAGACGGCTTGGTTTTGATAAGGTTTTTGA
TACCAACTTTACTGCTGACTTGACCATAATGGAAGAAGGTCATGAATTAATTGAAAGACTGAAAAACAATGAAAGGCTAC
CGTTGATTACTTCCTGTAGTCCGGGCTGGATTAAGTTTATTGAACACTTCTACCCAAGTTACCTTGAGCATATCTCAAGC
TGTAAATCTCCTCAACAGATGTTTGGGGCCCTTGCCAAAACTTATTACCCTGAAAATAATGGTATAGACCCGGAAGATGT
ATTTGTAGTTTCGGTTATGCCCTGTACTGCTAAAAAATTCGAAATAACAAGACCCGGTATGGATAGTAGTGGGTATCAGG
ACGTAGATGTGGTTCTTACCACAAGGGAGCTGGCAAAAATGTTTAAACAGGCCGGGATTGACTTTGTGAATCTCCCTGAT
GAAGAATATGATAAACCCCTCGGTATTTCGACTGGTGCCGGTACTATTTTTGGAACAACAGGTGGCGTTATGGAAGCAGC
CTTAAGAACTGCCTATGAGGTATTAACAGGGGAGGAATTACCCGGTCTGGAATTTGAGGATGTAAGGGGTTTAGAGGGGA
TTAAGGAATGTGAAATTGAAATTAACGGTCAGAAAATAAAAGTTGCCGTAGCTCATGGACTTTCCAATGCTCATAAGGTA
CTTCAAAATATAGACGACTATCATTTTATTGAAATTATGGCCTGCCCTGGTGGTTGTGTTGGTGGTGGTGGTCAGCCCTA
TCCTACCAATGAAGAAACTATAAGATTAAGGGCCCAGGGCCTTTACCGGGATGATAAGGAACATCAGATCAGGAAATCCC
ACGAAAATCCTGTTGTCAAAAAACTATATGAAGAATTTCTTGGCAAACCATTGAGTCATAAGTCTCATGAATTACTACAC
ACCGGGTATGTTGTAAGATCAAAATACCCGGCCAATGTTGAATCTGATGCGGTTTAA

Upstream 100 bases:

>100_bases
AAGAAGAGGCCCATGTAATCGATCTGGATAAATGTATTAAGTGTGGGTCCTGTCTTGATAAATGTCCATTTAATGCTGTT
CAGAAAGGAGCGTAATTGAA

Downstream 100 bases:

>100_bases
TAATAAATAAAAGAAAAGCCCTCTTAATAAGAGGGCTTTTTTTAATGTTCAAAAATTATATACAGGTGTTTTTATGGGTT
AATTTCAGGAATAAAAAATA

Product: NADH dehydrogenase (ubiquinone) 75 kDa subunit

Products: NA

Alternate protein names: CpI; Fe-only hydrogenase; [Fe] hydrogenase [H]

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MSDKVNITLDGKSLTVDKDKTILEVAREAGIKIPTLCYLEEINEIGSCRVCVVEVNGKIQPACVTPVSEGLEITTTSPRI
REARRISLELIISDHPMECLTCSRNGNCELQRLAEDFGISEITYEGEQSHFEPDLSSPSIVRDPDKCILCRRCVSVCEQV
QGVAALTPNERGFSTIITPAFGQKLGEIACANCGQCINACPVGALSEKDDTEKVWEALANPDKHVVVQTAPAVRVSIGEV
FGMKPGSLVTGKLMAGLRRLGFDKVFDTNFTADLTIMEEGHELIERLKNNERLPLITSCSPGWIKFIEHFYPSYLEHISS
CKSPQQMFGALAKTYYPENNGIDPEDVFVVSVMPCTAKKFEITRPGMDSSGYQDVDVVLTTRELAKMFKQAGIDFVNLPD
EEYDKPLGISTGAGTIFGTTGGVMEAALRTAYEVLTGEELPGLEFEDVRGLEGIKECEIEINGQKIKVAVAHGLSNAHKV
LQNIDDYHFIEIMACPGGCVGGGGQPYPTNEETIRLRAQGLYRDDKEHQIRKSHENPVVKKLYEEFLGKPLSHKSHELLH
TGYVVRSKYPANVESDAV

Sequences:

>Translated_578_residues
MSDKVNITLDGKSLTVDKDKTILEVAREAGIKIPTLCYLEEINEIGSCRVCVVEVNGKIQPACVTPVSEGLEITTTSPRI
REARRISLELIISDHPMECLTCSRNGNCELQRLAEDFGISEITYEGEQSHFEPDLSSPSIVRDPDKCILCRRCVSVCEQV
QGVAALTPNERGFSTIITPAFGQKLGEIACANCGQCINACPVGALSEKDDTEKVWEALANPDKHVVVQTAPAVRVSIGEV
FGMKPGSLVTGKLMAGLRRLGFDKVFDTNFTADLTIMEEGHELIERLKNNERLPLITSCSPGWIKFIEHFYPSYLEHISS
CKSPQQMFGALAKTYYPENNGIDPEDVFVVSVMPCTAKKFEITRPGMDSSGYQDVDVVLTTRELAKMFKQAGIDFVNLPD
EEYDKPLGISTGAGTIFGTTGGVMEAALRTAYEVLTGEELPGLEFEDVRGLEGIKECEIEINGQKIKVAVAHGLSNAHKV
LQNIDDYHFIEIMACPGGCVGGGGQPYPTNEETIRLRAQGLYRDDKEHQIRKSHENPVVKKLYEEFLGKPLSHKSHELLH
TGYVVRSKYPANVESDAV
>Mature_577_residues
SDKVNITLDGKSLTVDKDKTILEVAREAGIKIPTLCYLEEINEIGSCRVCVVEVNGKIQPACVTPVSEGLEITTTSPRIR
EARRISLELIISDHPMECLTCSRNGNCELQRLAEDFGISEITYEGEQSHFEPDLSSPSIVRDPDKCILCRRCVSVCEQVQ
GVAALTPNERGFSTIITPAFGQKLGEIACANCGQCINACPVGALSEKDDTEKVWEALANPDKHVVVQTAPAVRVSIGEVF
GMKPGSLVTGKLMAGLRRLGFDKVFDTNFTADLTIMEEGHELIERLKNNERLPLITSCSPGWIKFIEHFYPSYLEHISSC
KSPQQMFGALAKTYYPENNGIDPEDVFVVSVMPCTAKKFEITRPGMDSSGYQDVDVVLTTRELAKMFKQAGIDFVNLPDE
EYDKPLGISTGAGTIFGTTGGVMEAALRTAYEVLTGEELPGLEFEDVRGLEGIKECEIEINGQKIKVAVAHGLSNAHKVL
QNIDDYHFIEIMACPGGCVGGGGQPYPTNEETIRLRAQGLYRDDKEHQIRKSHENPVVKKLYEEFLGKPLSHKSHELLHT
GYVVRSKYPANVESDAV

Specific function: Ndh-1 Shuttles Electrons From NADH, Via Fmn And Iron- Sulfur (Fe-S) Centers, To Quinones In The Respiratory Chain. The Immediate Electron Acceptor For The Enzyme In This Species Is Believed To Be Ubiquinone. Couples The Redox Reaction To Proton Translocat

COG id: COG4624

COG function: function code R; Iron only hydrogenase large subunit, C-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI11968051, Length=403, Percent_Identity=30.7692307692308, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI6912524, Length=404, Percent_Identity=28.4653465346535, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI134284357, Length=336, Percent_Identity=29.7619047619048, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI84452151, Length=336, Percent_Identity=29.7619047619048, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI14165461, Length=374, Percent_Identity=27.807486631016, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI33519475, Length=254, Percent_Identity=29.9212598425197, Blast_Score=119, Evalue=8e-27,
Organism=Escherichia coli, GI145693161, Length=248, Percent_Identity=26.2096774193548, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI32566231, Length=256, Percent_Identity=28.90625, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17565758, Length=225, Percent_Identity=30.2222222222222, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI193209088, Length=256, Percent_Identity=28.90625, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71995015, Length=386, Percent_Identity=28.4974093264249, Blast_Score=111, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6324089, Length=419, Percent_Identity=24.582338902148, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI116007464, Length=325, Percent_Identity=29.5384615384615, Blast_Score=156, Evalue=4e-38,
Organism=Drosophila melanogaster, GI116007468, Length=325, Percent_Identity=29.5384615384615, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI116007470, Length=325, Percent_Identity=29.5384615384615, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI116007466, Length=325, Percent_Identity=29.5384615384615, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI116007462, Length=283, Percent_Identity=28.9752650176678, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24640559, Length=217, Percent_Identity=32.7188940092166, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24640557, Length=217, Percent_Identity=32.7188940092166, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012675
- InterPro:   IPR009016
- InterPro:   IPR004108
- InterPro:   IPR003149
- InterPro:   IPR013352
- InterPro:   IPR001041 [H]

Pfam domain/function: PF02906 Fe_hyd_lg_C; PF02256 Fe_hyd_SSU [H]

EC number: =1.12.7.2 [H]

Molecular weight: Translated: 63595; Mature: 63464

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00641 COMPLEX1_75K_1 ; PS51085 2FE2S_FER_2 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKVNITLDGKSLTVDKDKTILEVAREAGIKIPTLCYLEEINEIGSCRVCVVEVNGKIQ
CCCCEEEEECCCEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCC
PACVTPVSEGLEITTTSPRIREARRISLELIISDHPMECLTCSRNGNCELQRLAEDFGIS
CEEECCCCCCEEEEECCHHHHHHHEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHCCCC
EITYEGEQSHFEPDLSSPSIVRDPDKCILCRRCVSVCEQVQGVAALTPNERGFSTIITPA
EEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEECCH
FGQKLGEIACANCGQCINACPVGALSEKDDTEKVWEALANPDKHVVVQTAPAVRVSIGEV
HHHHHHHHHHHCHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCEEEEHHHH
FGMKPGSLVTGKLMAGLRRLGFDKVFDTNFTADLTIMEEGHELIERLKNNERLPLITSCS
HCCCCCCEEHHHHHHHHHHCCCHHHCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEECCC
PGWIKFIEHFYPSYLEHISSCKSPQQMFGALAKTYYPENNGIDPEDVFVVSVMPCTAKKF
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEE
EITRPGMDSSGYQDVDVVLTTRELAKMFKQAGIDFVNLPDEEYDKPLGISTGAGTIFGTT
EEECCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCEEECCCHHCCCCCCCCCCCCEEEECC
GGVMEAALRTAYEVLTGEELPGLEFEDVRGLEGIKECEIEINGQKIKVAVAHGLSNAHKV
CHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCEEEEEEECCCEEEEEEECCCHHHHHH
LQNIDDYHFIEIMACPGGCVGGGGQPYPTNEETIRLRAQGLYRDDKEHQIRKSHENPVVK
HHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCHHHH
KLYEEFLGKPLSHKSHELLHTGYVVRSKYPANVESDAV
HHHHHHCCCCCCCCCHHHHHHCEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SDKVNITLDGKSLTVDKDKTILEVAREAGIKIPTLCYLEEINEIGSCRVCVVEVNGKIQ
CCCEEEEECCCEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCC
PACVTPVSEGLEITTTSPRIREARRISLELIISDHPMECLTCSRNGNCELQRLAEDFGIS
CEEECCCCCCEEEEECCHHHHHHHEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHCCCC
EITYEGEQSHFEPDLSSPSIVRDPDKCILCRRCVSVCEQVQGVAALTPNERGFSTIITPA
EEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEECCH
FGQKLGEIACANCGQCINACPVGALSEKDDTEKVWEALANPDKHVVVQTAPAVRVSIGEV
HHHHHHHHHHHCHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCEEEEHHHH
FGMKPGSLVTGKLMAGLRRLGFDKVFDTNFTADLTIMEEGHELIERLKNNERLPLITSCS
HCCCCCCEEHHHHHHHHHHCCCHHHCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEECCC
PGWIKFIEHFYPSYLEHISSCKSPQQMFGALAKTYYPENNGIDPEDVFVVSVMPCTAKKF
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEE
EITRPGMDSSGYQDVDVVLTTRELAKMFKQAGIDFVNLPDEEYDKPLGISTGAGTIFGTT
EEECCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCEEECCCHHCCCCCCCCCCCCEEEECC
GGVMEAALRTAYEVLTGEELPGLEFEDVRGLEGIKECEIEINGQKIKVAVAHGLSNAHKV
CHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCEEEEEEECCCEEEEEEECCCHHHHHH
LQNIDDYHFIEIMACPGGCVGGGGQPYPTNEETIRLRAQGLYRDDKEHQIRKSHENPVVK
HHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCHHHH
KLYEEFLGKPLSHKSHELLHTGYVVRSKYPANVESDAV
HHHHHHCCCCCCCCCHHHHHHCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1911757; 9836629; 10529166 [H]