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The map label for this gene is nuoF2 [H]

Identifier: 220931476

GI number: 220931476

Start: 680665

End: 682449

Strand: Direct

Name: nuoF2 [H]

Synonym: Hore_06320

Alternate gene names: 220931476

Gene position: 680665-682449 (Clockwise)

Preceding gene: 220931475

Following gene: 220931477

Centisome position: 26.4

GC content: 40.95

Gene sequence:

>1785_bases
ATGAAGAAATATACGATATGCGTCTGTGGGGGAACCGGATGTTTATCGTCCGAAAGTGAAGACGTTAAAAATGAGTTTAT
TACCCTGATAGAAAAAGAAGGTTTAACAGAACAGGTTACTGTTAAAGAGACAGGTTGTATGGGAACCTGTGATCTTGGTC
CGGTAGCTTTAATTGACCCGGACAATGTTTTTTATTGTAAGCTTAAACCAGAGGATGTTTCTGATATTATTAATTCCCAT
ATTAAACAGGGGAGTATAGTAGAACGTCTGGTATTCGGTTATGATAAAGATAATCCCATAAGTGAACATGATGATATTCC
CTTCTTTAAAGGGCAGAAAAAAATTGCCTTAAGGAATACCGGGGTAATTGATCCCGATAATATTGAAGATTATTTATCCC
ATGACGGATATAAGGCCCTGGAGAAAGTCTTAACTGAAATGTCTCCAGAGGAAGTAATTGAAGAAATAAAAGAATCAGGA
TTAAGGGGCCGTGGTGGTGGCGGTTTCCCGACAGGATTAAAATGGCAATTTACCGCCCAGTCTGAAGGAGAACAAAAATA
TATAGTCTGTAATGCAGATGAAGGTGACCCTGGTGCCTTTATGGATAGAAGTATACTGGAGGGGGATCCTCACAGTATAA
TTGAAGCTATGGCTATTGCCGGTTATGCTGTAGGAGCCAATCAGGGTTATGTCTATGTCCGGGCAGAGTACCCTCTGGCT
GTAAAGAGGATGGAAAAGGCTATATCTGATGCCAGGGAAAATGGATTTATTGGAAATAATATATTTGATACTAATTTTAA
GTTTGACCTGGAGATTAGAGTTGGGGCCGGTGCTTTTGTTTGTGGTGAAGAAACGGCTCTTATGGCTTCCATTGAAGGTG
AAAGGGGAGAACCACGTCCCAAGCCACCATTTCCGGCCCAGTCTGGTTTATGGGGAAAACCAACCCTTATTAATAATGTA
GAGACCTATGCCAATATACCGGTTATTATTTTAAAGGGGGCTAAATGGTTTAACCATATTGGTACTGAAAAAAGTAAAGG
TACTAAAGTGTTTGCTGTAGCCGGTGATATTAATACTACCGGGCTTGTTGAAGTTCCGATGGGTACTACCCTAAGAGAGA
TAATTTTTGATATTTGTGGAGGAATACCGGAAGGCAAGAAGTTTAAAGCAGCCCAGACCGGGGGACCATCCGGGGGTTGT
ATCCCCAGGGAACACCTTGATATCAGTATGGATTATGATTCCCTGATTGAAATTGGCTCAATGATGGGATCCGGTGGATT
AATCGTTATGGATGAAAATACCTGTATGGTTGACGTTGCCAGGTTTTTTCTCGATTTTACCCAGGATGAATCATGTGGCA
AGTGTGTCCCCTGTCGGATTGGAACCAAAAGGATGCTTGAAATATTAGAAAGAATTACAAAAGGTCAGGGAGAACCTGGA
GATATTGAAAAGTTAGAGAAACTGGGTAAATATATAAAAGAAAATGCCCTGTGTGGTCTGGGGCAAACTGCCCCTAATCC
TGTCCTGAGTACAATTAAGTACTTCAGGGATGAATATGAAGCCCATATTAAAGATAAAAATTGTCCGGCCGGAAGTTGTC
GCAATTTAACCAGGTATAAAATTATAGCCGAAGAATGCCGTAACTGTGGTCTATGTGCTAAAGTCTGCCCGGTTGATGCT
ATTACCAAAGAAGAAGAGGCCCATGTAATCGATCTGGATAAATGTATTAAGTGTGGGTCCTGTCTTGATAAATGTCCATT
TAATGCTGTTCAGAAAGGAGCGTAA

Upstream 100 bases:

>100_bases
GATGGAGATTATTGAATCCTATCGTAAATAATATATTTTTTGATGTGATAAATATATCTTTCACAAACTTAATAATATCT
GTTTTTAAGGAGGAAAATCG

Downstream 100 bases:

>100_bases
TTGAAATGTCTGATAAAGTAAATATAACATTAGATGGTAAAAGTTTAACTGTAGATAAAGATAAAACTATTCTGGAAGTA
GCCCGGGAAGCCGGGATTAA

Product: NADH dehydrogenase (ubiquinone) 51 kDa subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MKKYTICVCGGTGCLSSESEDVKNEFITLIEKEGLTEQVTVKETGCMGTCDLGPVALIDPDNVFYCKLKPEDVSDIINSH
IKQGSIVERLVFGYDKDNPISEHDDIPFFKGQKKIALRNTGVIDPDNIEDYLSHDGYKALEKVLTEMSPEEVIEEIKESG
LRGRGGGGFPTGLKWQFTAQSEGEQKYIVCNADEGDPGAFMDRSILEGDPHSIIEAMAIAGYAVGANQGYVYVRAEYPLA
VKRMEKAISDARENGFIGNNIFDTNFKFDLEIRVGAGAFVCGEETALMASIEGERGEPRPKPPFPAQSGLWGKPTLINNV
ETYANIPVIILKGAKWFNHIGTEKSKGTKVFAVAGDINTTGLVEVPMGTTLREIIFDICGGIPEGKKFKAAQTGGPSGGC
IPREHLDISMDYDSLIEIGSMMGSGGLIVMDENTCMVDVARFFLDFTQDESCGKCVPCRIGTKRMLEILERITKGQGEPG
DIEKLEKLGKYIKENALCGLGQTAPNPVLSTIKYFRDEYEAHIKDKNCPAGSCRNLTRYKIIAEECRNCGLCAKVCPVDA
ITKEEEAHVIDLDKCIKCGSCLDKCPFNAVQKGA

Sequences:

>Translated_594_residues
MKKYTICVCGGTGCLSSESEDVKNEFITLIEKEGLTEQVTVKETGCMGTCDLGPVALIDPDNVFYCKLKPEDVSDIINSH
IKQGSIVERLVFGYDKDNPISEHDDIPFFKGQKKIALRNTGVIDPDNIEDYLSHDGYKALEKVLTEMSPEEVIEEIKESG
LRGRGGGGFPTGLKWQFTAQSEGEQKYIVCNADEGDPGAFMDRSILEGDPHSIIEAMAIAGYAVGANQGYVYVRAEYPLA
VKRMEKAISDARENGFIGNNIFDTNFKFDLEIRVGAGAFVCGEETALMASIEGERGEPRPKPPFPAQSGLWGKPTLINNV
ETYANIPVIILKGAKWFNHIGTEKSKGTKVFAVAGDINTTGLVEVPMGTTLREIIFDICGGIPEGKKFKAAQTGGPSGGC
IPREHLDISMDYDSLIEIGSMMGSGGLIVMDENTCMVDVARFFLDFTQDESCGKCVPCRIGTKRMLEILERITKGQGEPG
DIEKLEKLGKYIKENALCGLGQTAPNPVLSTIKYFRDEYEAHIKDKNCPAGSCRNLTRYKIIAEECRNCGLCAKVCPVDA
ITKEEEAHVIDLDKCIKCGSCLDKCPFNAVQKGA
>Mature_594_residues
MKKYTICVCGGTGCLSSESEDVKNEFITLIEKEGLTEQVTVKETGCMGTCDLGPVALIDPDNVFYCKLKPEDVSDIINSH
IKQGSIVERLVFGYDKDNPISEHDDIPFFKGQKKIALRNTGVIDPDNIEDYLSHDGYKALEKVLTEMSPEEVIEEIKESG
LRGRGGGGFPTGLKWQFTAQSEGEQKYIVCNADEGDPGAFMDRSILEGDPHSIIEAMAIAGYAVGANQGYVYVRAEYPLA
VKRMEKAISDARENGFIGNNIFDTNFKFDLEIRVGAGAFVCGEETALMASIEGERGEPRPKPPFPAQSGLWGKPTLINNV
ETYANIPVIILKGAKWFNHIGTEKSKGTKVFAVAGDINTTGLVEVPMGTTLREIIFDICGGIPEGKKFKAAQTGGPSGGC
IPREHLDISMDYDSLIEIGSMMGSGGLIVMDENTCMVDVARFFLDFTQDESCGKCVPCRIGTKRMLEILERITKGQGEPG
DIEKLEKLGKYIKENALCGLGQTAPNPVLSTIKYFRDEYEAHIKDKNCPAGSCRNLTRYKIIAEECRNCGLCAKVCPVDA
ITKEEEAHVIDLDKCIKCGSCLDKCPFNAVQKGA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=393, Percent_Identity=41.9847328244275, Blast_Score=316, Evalue=4e-86,
Organism=Homo sapiens, GI260656005, Length=393, Percent_Identity=41.9847328244275, Blast_Score=316, Evalue=4e-86,
Organism=Escherichia coli, GI1788620, Length=404, Percent_Identity=43.5643564356436, Blast_Score=372, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17531727, Length=395, Percent_Identity=40.5063291139241, Blast_Score=304, Evalue=7e-83,
Organism=Drosophila melanogaster, GI19920794, Length=393, Percent_Identity=41.9847328244275, Blast_Score=317, Evalue=2e-86,
Organism=Drosophila melanogaster, GI45550452, Length=395, Percent_Identity=40.7594936708861, Blast_Score=299, Evalue=3e-81,
Organism=Drosophila melanogaster, GI24653819, Length=385, Percent_Identity=32.2077922077922, Blast_Score=228, Evalue=7e-60,
Organism=Drosophila melanogaster, GI24653817, Length=355, Percent_Identity=30.1408450704225, Blast_Score=194, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 64861; Mature: 64861

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00645 COMPLEX1_51K_2 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYTICVCGGTGCLSSESEDVKNEFITLIEKEGLTEQVTVKETGCMGTCDLGPVALIDP
CCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCEEEECC
DNVFYCKLKPEDVSDIINSHIKQGSIVERLVFGYDKDNPISEHDDIPFFKGQKKIALRNT
CCEEEEEECCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECC
GVIDPDNIEDYLSHDGYKALEKVLTEMSPEEVIEEIKESGLRGRGGGGFPTGLKWQFTAQ
CCCCCCCHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECC
SEGEQKYIVCNADEGDPGAFMDRSILEGDPHSIIEAMAIAGYAVGANQGYVYVRAEYPLA
CCCCEEEEEEECCCCCCCHHHCCHHHCCCHHHHHHHHHHHHEEEECCCCEEEEEECCCHH
VKRMEKAISDARENGFIGNNIFDTNFKFDLEIRVGAGAFVCGEETALMASIEGERGEPRP
HHHHHHHHHHHHHCCCCCCCEECCCEEEEEEEEECCCEEEECCCCEEEEEECCCCCCCCC
KPPFPAQSGLWGKPTLINNVETYANIPVIILKGAKWFNHIGTEKSKGTKVFAVAGDINTT
CCCCCCCCCCCCCCCHHCCCHHHCCCCEEEEECCHHHHHCCCCCCCCCEEEEEECCCCCC
GLVEVPMGTTLREIIFDICGGIPEGKKFKAAQTGGPSGGCIPREHLDISMDYDSLIEIGS
CEEEECCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCHHCCEEEECHHHHHHHHH
MMGSGGLIVMDENTCMVDVARFFLDFTQDESCGKCVPCRIGTKRMLEILERITKGQGEPG
HCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHCCCCCCC
DIEKLEKLGKYIKENALCGLGQTAPNPVLSTIKYFRDEYEAHIKDKNCPAGSCRNLTRYK
CHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
IIAEECRNCGLCAKVCPVDAITKEEEAHVIDLDKCIKCGSCLDKCPFNAVQKGA
HHHHHHHCCCCCHHHCCCHHHCCCCCCCEECHHHHHHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure
MKKYTICVCGGTGCLSSESEDVKNEFITLIEKEGLTEQVTVKETGCMGTCDLGPVALIDP
CCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCEEEECC
DNVFYCKLKPEDVSDIINSHIKQGSIVERLVFGYDKDNPISEHDDIPFFKGQKKIALRNT
CCEEEEEECCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECC
GVIDPDNIEDYLSHDGYKALEKVLTEMSPEEVIEEIKESGLRGRGGGGFPTGLKWQFTAQ
CCCCCCCHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECC
SEGEQKYIVCNADEGDPGAFMDRSILEGDPHSIIEAMAIAGYAVGANQGYVYVRAEYPLA
CCCCEEEEEEECCCCCCCHHHCCHHHCCCHHHHHHHHHHHHEEEECCCCEEEEEECCCHH
VKRMEKAISDARENGFIGNNIFDTNFKFDLEIRVGAGAFVCGEETALMASIEGERGEPRP
HHHHHHHHHHHHHCCCCCCCEECCCEEEEEEEEECCCEEEECCCCEEEEEECCCCCCCCC
KPPFPAQSGLWGKPTLINNVETYANIPVIILKGAKWFNHIGTEKSKGTKVFAVAGDINTT
CCCCCCCCCCCCCCCHHCCCHHHCCCCEEEEECCHHHHHCCCCCCCCCEEEEEECCCCCC
GLVEVPMGTTLREIIFDICGGIPEGKKFKAAQTGGPSGGCIPREHLDISMDYDSLIEIGS
CEEEECCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCHHCCEEEECHHHHHHHHH
MMGSGGLIVMDENTCMVDVARFFLDFTQDESCGKCVPCRIGTKRMLEILERITKGQGEPG
HCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHCCCCCCC
DIEKLEKLGKYIKENALCGLGQTAPNPVLSTIKYFRDEYEAHIKDKNCPAGSCRNLTRYK
CHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
IIAEECRNCGLCAKVCPVDAITKEEEAHVIDLDKCIKCGSCLDKCPFNAVQKGA
HHHHHHHCCCCCHHHCCCHHHCCCCCCCEECHHHHHHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]