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The map label for this gene is sbcD [H]

Identifier: 220931248

GI number: 220931248

Start: 412184

End: 413491

Strand: Direct

Name: sbcD [H]

Synonym: Hore_04020

Alternate gene names: 220931248

Gene position: 412184-413491 (Clockwise)

Preceding gene: 220931247

Following gene: 220931249

Centisome position: 15.99

GC content: 42.74

Gene sequence:

>1308_bases
ATGGACTGTATACAAAATCAATATAAGTTTATTGTACATGAACTTATAACTGTTGTTATAAAAAGGGGGAGAGGTCCTTT
GAGAATTTTACATACTGCTGACTGGCATCTGGGGAAACACCTGGAGGGATGGAGTAGATATGAAGAACAAAAAGAATTTG
TTGAAGAAATAATTGAAATAGCTGATGATAATAAGGTAGACATGGTTTTAATATGTGGGGATATATTTGACACTACTAAC
CCTCCAGCGGAGGCGGAACAGCTTTTTTTTCAGGCGATGGATTACCTGTCAAAAGGTGGGGAGAGGGTAATCTGTTTGAT
CTCTGGTAACCATGATAGTCCCAACCGTCTTATGGCCCCGGGGCCTCTGGCTTCCAGACAGGGAATTTTTATTATGGATG
AGCCCCGGGGAGACAGGTATAAGCTGGATGATGACCGGGTGTTAAACCGTGGTCAGGGGTACATAGAACTTGAAATTAAC
GGAGAAGGTGTTGTCCTTACGGCTCTGCCCTATCCTTCTGAGAGCCGGTTAAACCAGGTCTTTTCATGGACCGGTGATGA
CCGGGCAGTGCAGGAAAGTTATTCCCGTCGAGTGGGTCAGATTTTTTCCCATTTAGAGCAATATTATCGTGAAAATACAA
TCAACATTGCCATGAGTCACCTTTTCGTTGCCGGGGGTCAGACTACCCGGTCTGAAAGACCCATCCAGGTTGGTGGCAGC
CTGACAGTATTACCGGAACACCTTCCAGAAAAATCCCAGTACACAGCCCTGGGCCACCTCCATCGTTATCAAATTGCTTC
TTCAGCCCGGAGGGCTTACTATTCAGGTTCTCCGTTGCAGTATAGCCTCAGTGAAAAAGATCATAAAAAGTGTGTTAACC
TTGTAGAGCTTCATCCGGGAGAGGAGGCCCGGATTGAACAGGTTGAATTGACAACAAAAAAACCAATCGAGGTCTGGGAA
GCAGAAGGGGTTGAAGAGGCTATAAAAATGGTTGAGGCCAATAAGGACCGTTCTGTCTGGGCATACCTTTATATAAATGT
GGATAAAACTCTACTTCAGTCTGAAATTAAAAAAATAAAGGAAATTAAAAAGGATATTCTATGTATTAACCCCATAACCC
CTGAAGAGAAATATGAATGGGAAACTATAAAGATGCCTGATGAGGACCTGGATATAATGGAATTGTTTAAAGAATATTAT
CGTAAGACAAAAAAAGTTGAGCCTGATGACGATATTATCAGGATGTTTAGCAGTATTGTTAATGATACCAGGGAAAAGGG
GGAGCATGATGAGACCGCTGCTTCTTAA

Upstream 100 bases:

>100_bases
TATCTTATATATTATCAGGAAGAAGAGGAAGGATTTTTTATAATAAACAAGAATTATATAATGATAACAAAACTTATGTT
CACTTTAAAATGTGGTTTTG

Downstream 100 bases:

>100_bases
AATTTCGGGTTTAAATAGCTTTGTCCAGGAAGAGATTATTAATTTTAAAAAGCTGACCGAAAAAGGGTTATTTGGTATTT
TTGGTCCCACCGGGAGTGGT

Product: Exodeoxyribonuclease I subunit D

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MDCIQNQYKFIVHELITVVIKRGRGPLRILHTADWHLGKHLEGWSRYEEQKEFVEEIIEIADDNKVDMVLICGDIFDTTN
PPAEAEQLFFQAMDYLSKGGERVICLISGNHDSPNRLMAPGPLASRQGIFIMDEPRGDRYKLDDDRVLNRGQGYIELEIN
GEGVVLTALPYPSESRLNQVFSWTGDDRAVQESYSRRVGQIFSHLEQYYRENTINIAMSHLFVAGGQTTRSERPIQVGGS
LTVLPEHLPEKSQYTALGHLHRYQIASSARRAYYSGSPLQYSLSEKDHKKCVNLVELHPGEEARIEQVELTTKKPIEVWE
AEGVEEAIKMVEANKDRSVWAYLYINVDKTLLQSEIKKIKEIKKDILCINPITPEEKYEWETIKMPDEDLDIMELFKEYY
RKTKKVEPDDDIIRMFSSIVNDTREKGEHDETAAS

Sequences:

>Translated_435_residues
MDCIQNQYKFIVHELITVVIKRGRGPLRILHTADWHLGKHLEGWSRYEEQKEFVEEIIEIADDNKVDMVLICGDIFDTTN
PPAEAEQLFFQAMDYLSKGGERVICLISGNHDSPNRLMAPGPLASRQGIFIMDEPRGDRYKLDDDRVLNRGQGYIELEIN
GEGVVLTALPYPSESRLNQVFSWTGDDRAVQESYSRRVGQIFSHLEQYYRENTINIAMSHLFVAGGQTTRSERPIQVGGS
LTVLPEHLPEKSQYTALGHLHRYQIASSARRAYYSGSPLQYSLSEKDHKKCVNLVELHPGEEARIEQVELTTKKPIEVWE
AEGVEEAIKMVEANKDRSVWAYLYINVDKTLLQSEIKKIKEIKKDILCINPITPEEKYEWETIKMPDEDLDIMELFKEYY
RKTKKVEPDDDIIRMFSSIVNDTREKGEHDETAAS
>Mature_435_residues
MDCIQNQYKFIVHELITVVIKRGRGPLRILHTADWHLGKHLEGWSRYEEQKEFVEEIIEIADDNKVDMVLICGDIFDTTN
PPAEAEQLFFQAMDYLSKGGERVICLISGNHDSPNRLMAPGPLASRQGIFIMDEPRGDRYKLDDDRVLNRGQGYIELEIN
GEGVVLTALPYPSESRLNQVFSWTGDDRAVQESYSRRVGQIFSHLEQYYRENTINIAMSHLFVAGGQTTRSERPIQVGGS
LTVLPEHLPEKSQYTALGHLHRYQIASSARRAYYSGSPLQYSLSEKDHKKCVNLVELHPGEEARIEQVELTTKKPIEVWE
AEGVEEAIKMVEANKDRSVWAYLYINVDKTLLQSEIKKIKEIKKDILCINPITPEEKYEWETIKMPDEDLDIMELFKEYY
RKTKKVEPDDDIIRMFSSIVNDTREKGEHDETAAS

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=358, Percent_Identity=26.536312849162, Blast_Score=118, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 50113; Mature: 50113

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDCIQNQYKFIVHELITVVIKRGRGPLRILHTADWHLGKHLEGWSRYEEQKEFVEEIIEI
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHCCCCHHHHHHHHHHHHHHHHHHH
ADDNKVDMVLICGDIFDTTNPPAEAEQLFFQAMDYLSKGGERVICLISGNHDSPNRLMAP
CCCCCEEEEEEECCHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEECC
GPLASRQGIFIMDEPRGDRYKLDDDRVLNRGQGYIELEINGEGVVLTALPYPSESRLNQV
CCCCCCCCEEEEECCCCCCEECCHHHHHHCCCCEEEEEECCCEEEEEECCCCCHHHHHHH
FSWTGDDRAVQESYSRRVGQIFSHLEQYYRENTINIAMSHLFVAGGQTTRSERPIQVGGS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEHHHHEECCCCCCCCCCEEECCC
LTVLPEHLPEKSQYTALGHLHRYQIASSARRAYYSGSPLQYSLSEKDHKKCVNLVELHPG
EEECHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCC
EEARIEQVELTTKKPIEVWEAEGVEEAIKMVEANKDRSVWAYLYINVDKTLLQSEIKKIK
CCCCEEEEEECCCCCCHHHHCCCHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHHH
EIKKDILCINPITPEEKYEWETIKMPDEDLDIMELFKEYYRKTKKVEPDDDIIRMFSSIV
HHHHHEEEECCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
NDTREKGEHDETAAS
HHHHHCCCCCCCCCC
>Mature Secondary Structure
MDCIQNQYKFIVHELITVVIKRGRGPLRILHTADWHLGKHLEGWSRYEEQKEFVEEIIEI
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHCCCCHHHHHHHHHHHHHHHHHHH
ADDNKVDMVLICGDIFDTTNPPAEAEQLFFQAMDYLSKGGERVICLISGNHDSPNRLMAP
CCCCCEEEEEEECCHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEECC
GPLASRQGIFIMDEPRGDRYKLDDDRVLNRGQGYIELEINGEGVVLTALPYPSESRLNQV
CCCCCCCCEEEEECCCCCCEECCHHHHHHCCCCEEEEEECCCEEEEEECCCCCHHHHHHH
FSWTGDDRAVQESYSRRVGQIFSHLEQYYRENTINIAMSHLFVAGGQTTRSERPIQVGGS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEHHHHEECCCCCCCCCCEEECCC
LTVLPEHLPEKSQYTALGHLHRYQIASSARRAYYSGSPLQYSLSEKDHKKCVNLVELHPG
EEECHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCC
EEARIEQVELTTKKPIEVWEAEGVEEAIKMVEANKDRSVWAYLYINVDKTLLQSEIKKIK
CCCCEEEEEECCCCCCHHHHCCCHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHHH
EIKKDILCINPITPEEKYEWETIKMPDEDLDIMELFKEYYRKTKKVEPDDDIIRMFSSIV
HHHHHEEEECCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
NDTREKGEHDETAAS
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10568751; 1646786; 8493111 [H]