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The map label for this gene is pyk [H]

Identifier: 220931170

GI number: 220931170

Start: 330449

End: 332203

Strand: Direct

Name: pyk [H]

Synonym: Hore_03220

Alternate gene names: 220931170

Gene position: 330449-332203 (Clockwise)

Preceding gene: 220931169

Following gene: 220931172

Centisome position: 12.82

GC content: 40.57

Gene sequence:

>1755_bases
ATGAGGAAAACAAAGATTGTGTGTACGATTGGACCTGTTAGTGAAAGCAAAGAAATGCTGAAAAAACTTGCAGAAGCTGG
TATGAATGTAGCCAGGCTTAACTTCTCCCATGGTGACCATGAAGAACATGGAAATCGAATTGATACCATCCGTAAAGTAG
AGGCTGAACTTGGTAAACCAATTGGAATTATGCTGGATACAAAAGGTCCAGAAATCCGTACTGGTATGTTAAAGGATGAT
AAGGTTGAATTAAAAAAGGGAGAAGAGATTATTCTGACTACAGAAGACATAGAAGGCGATGAGAACAGGGTTTCTGTAAG
TTATAAAGGCCTTCCTGATGATCTCCATGAAGGGGCTACTGTTTTAATTGATGATGGATTGATTGGTTTAGAGGTATTGG
AGATTAAGGGTACCGAAATAAGATGTAAAATTGTAAATGGTGGTCTCCTTGGCTCCAGAAAAGGGGTAAATCTTCCTGGA
ATATCTGTAAATTTACCGGCCCTGACCGAAAAAGATGAAAGTGATATTAGATTTGGAGTAAGAAAAGGTATTCATTTTAT
TGCAGCCTCTTTTGTTAGAAAGGCCCAGGATGTTATCGAAATTCGTAAAATACTGGAAGAAGAAGGAAAAGAAGATATAC
TTATTATTGCCAAGATAGAAAACCAGGAAGGTGTCGATAATATTGATGAAATAATTGATGTGGCTGATGGAATTATGGTT
GCCAGGGGTGACCTGGGGGTTGAGATTCCTGCAGAACAGGTACCTGTAATCCAGAAGTCTATCATCAAAAAGTGTAATGA
AAAGGCAAAACCGGTTATTACTGCAACTCAAATGTTGGATTCAATGATAAGAAATCCGAGACCAACAAGGGCCGAAGCTT
CCGATGTGGCCAATGCTATTTTTGACGGCACTGATGCTACTATGCTTTCTGGAGAATCAGCTGCCGGTGATTATCCGGTA
GAAGCAGTAAAGACTATGGCACGTATAGCCGAAGAGACAGAAAAATCCCTTTATTACCGGGATGTAATAAGCAATAGAAG
GACCTATAGACCTCAGACTGTGACCGATGCTATCAGTTTTGCTTCTTGTGAAACTGCAACTGATTTAGGAGCCCAGGCCA
TTATTACATCTACCGAGTCTGGTTTAACAGCCAGGATGGTGTCAAGATACAGGCCATTGGTACCTATTGTTGCTGTTACC
CCTGATGAGAGGGTTCAACATGCCTTAACTGTCAGCTGGGGTGTTTATCCATTAACAGTTAAGAAGAGTAACTCTACTGA
TGAAATGATGGATGTTTCTATAAAGACAGCCCAGGAGAACAGATTAATTAAAAGTGGTGATTTAGTTGTAATTACAGCCG
GTGCCCCAGTAGGTATACCGGGAACTACTAATCTTATTAAAGTTGATGTCGTTGGTGAACCACTGGTTGAAGGTCAGGGT
GTTGGCAAAGGAATTGTAACCGGTCAGGTTAAAATTGCTCATACTGCTAAAGAAGCTGTTGAAAAAATTGAAGAAGGGGA
TATACTGGTAACCGGTATTACCGATAAGGAATATTTACCTGCTATGAAAAAAGCAGGGGCTATAATAGCTGAACAGGGGG
GTCTAACATCACATCCCGCTATTGTGGGGTTAGAACTGGGTATTCCCGTAATAGTAAAGGCTGAAAAGGCTACTGAAAAT
CTTGTAGAAGGAGAAATTATAACTATCGATGGAGTACGGGGCCTGGTTTACCCTGGAACTGTTCATCTAAAATAG

Upstream 100 bases:

>100_bases
AACTGCCCTTTTTACCAGGTTTCCTACATAATTGTGTTATAACCTGAATACCCGGATAATGTTAGATTACAATATATTCA
ATTTATGGAGGTAATAAGGT

Downstream 100 bases:

>100_bases
GAGCAAAGACAAAGGAGAAGTAGCTGATTTGGGTATTTGTGTACTAAGATAAGTAGATAATTTACCCTGTGATAATAATT
ATTAGATATAAATTAAATAA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK; Vegetative protein 17; VEG17 [H]

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MRKTKIVCTIGPVSESKEMLKKLAEAGMNVARLNFSHGDHEEHGNRIDTIRKVEAELGKPIGIMLDTKGPEIRTGMLKDD
KVELKKGEEIILTTEDIEGDENRVSVSYKGLPDDLHEGATVLIDDGLIGLEVLEIKGTEIRCKIVNGGLLGSRKGVNLPG
ISVNLPALTEKDESDIRFGVRKGIHFIAASFVRKAQDVIEIRKILEEEGKEDILIIAKIENQEGVDNIDEIIDVADGIMV
ARGDLGVEIPAEQVPVIQKSIIKKCNEKAKPVITATQMLDSMIRNPRPTRAEASDVANAIFDGTDATMLSGESAAGDYPV
EAVKTMARIAEETEKSLYYRDVISNRRTYRPQTVTDAISFASCETATDLGAQAIITSTESGLTARMVSRYRPLVPIVAVT
PDERVQHALTVSWGVYPLTVKKSNSTDEMMDVSIKTAQENRLIKSGDLVVITAGAPVGIPGTTNLIKVDVVGEPLVEGQG
VGKGIVTGQVKIAHTAKEAVEKIEEGDILVTGITDKEYLPAMKKAGAIIAEQGGLTSHPAIVGLELGIPVIVKAEKATEN
LVEGEIITIDGVRGLVYPGTVHLK

Sequences:

>Translated_584_residues
MRKTKIVCTIGPVSESKEMLKKLAEAGMNVARLNFSHGDHEEHGNRIDTIRKVEAELGKPIGIMLDTKGPEIRTGMLKDD
KVELKKGEEIILTTEDIEGDENRVSVSYKGLPDDLHEGATVLIDDGLIGLEVLEIKGTEIRCKIVNGGLLGSRKGVNLPG
ISVNLPALTEKDESDIRFGVRKGIHFIAASFVRKAQDVIEIRKILEEEGKEDILIIAKIENQEGVDNIDEIIDVADGIMV
ARGDLGVEIPAEQVPVIQKSIIKKCNEKAKPVITATQMLDSMIRNPRPTRAEASDVANAIFDGTDATMLSGESAAGDYPV
EAVKTMARIAEETEKSLYYRDVISNRRTYRPQTVTDAISFASCETATDLGAQAIITSTESGLTARMVSRYRPLVPIVAVT
PDERVQHALTVSWGVYPLTVKKSNSTDEMMDVSIKTAQENRLIKSGDLVVITAGAPVGIPGTTNLIKVDVVGEPLVEGQG
VGKGIVTGQVKIAHTAKEAVEKIEEGDILVTGITDKEYLPAMKKAGAIIAEQGGLTSHPAIVGLELGIPVIVKAEKATEN
LVEGEIITIDGVRGLVYPGTVHLK
>Mature_584_residues
MRKTKIVCTIGPVSESKEMLKKLAEAGMNVARLNFSHGDHEEHGNRIDTIRKVEAELGKPIGIMLDTKGPEIRTGMLKDD
KVELKKGEEIILTTEDIEGDENRVSVSYKGLPDDLHEGATVLIDDGLIGLEVLEIKGTEIRCKIVNGGLLGSRKGVNLPG
ISVNLPALTEKDESDIRFGVRKGIHFIAASFVRKAQDVIEIRKILEEEGKEDILIIAKIENQEGVDNIDEIIDVADGIMV
ARGDLGVEIPAEQVPVIQKSIIKKCNEKAKPVITATQMLDSMIRNPRPTRAEASDVANAIFDGTDATMLSGESAAGDYPV
EAVKTMARIAEETEKSLYYRDVISNRRTYRPQTVTDAISFASCETATDLGAQAIITSTESGLTARMVSRYRPLVPIVAVT
PDERVQHALTVSWGVYPLTVKKSNSTDEMMDVSIKTAQENRLIKSGDLVVITAGAPVGIPGTTNLIKVDVVGEPLVEGQG
VGKGIVTGQVKIAHTAKEAVEKIEEGDILVTGITDKEYLPAMKKAGAIIAEQGGLTSHPAIVGLELGIPVIVKAEKATEN
LVEGEIITIDGVRGLVYPGTVHLK

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=487, Percent_Identity=45.1745379876797, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI33286420, Length=487, Percent_Identity=45.1745379876797, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI33286418, Length=487, Percent_Identity=45.3798767967146, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI10835121, Length=487, Percent_Identity=45.9958932238193, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI32967597, Length=487, Percent_Identity=45.9958932238193, Blast_Score=404, Evalue=1e-113,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=50, Blast_Score=267, Evalue=2e-71,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=50, Blast_Score=267, Evalue=2e-71,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=47.8813559322034, Blast_Score=209, Evalue=7e-54,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=47.8813559322034, Blast_Score=209, Evalue=7e-54,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=46.5116279069767, Blast_Score=182, Evalue=9e-46,
Organism=Escherichia coli, GI1787965, Length=473, Percent_Identity=53.6997885835095, Blast_Score=480, Evalue=1e-136,
Organism=Escherichia coli, GI1788160, Length=474, Percent_Identity=40.7172995780591, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1787994, Length=109, Percent_Identity=44.0366972477064, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17544584, Length=482, Percent_Identity=45.4356846473029, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI71984413, Length=484, Percent_Identity=47.1074380165289, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17506829, Length=484, Percent_Identity=47.1074380165289, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI71984406, Length=484, Percent_Identity=47.1074380165289, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17506831, Length=484, Percent_Identity=47.1074380165289, Blast_Score=386, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6319279, Length=486, Percent_Identity=45.2674897119342, Blast_Score=392, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324923, Length=487, Percent_Identity=42.7104722792608, Blast_Score=370, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24648964, Length=487, Percent_Identity=48.0492813141684, Blast_Score=413, Evalue=1e-115,
Organism=Drosophila melanogaster, GI28571814, Length=487, Percent_Identity=48.0492813141684, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24648966, Length=409, Percent_Identity=48.4107579462103, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24581235, Length=484, Percent_Identity=36.9834710743802, Blast_Score=298, Evalue=9e-81,
Organism=Drosophila melanogaster, GI24646914, Length=272, Percent_Identity=47.7941176470588, Blast_Score=231, Evalue=1e-60,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 62904; Mature: 62904

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTKIVCTIGPVSESKEMLKKLAEAGMNVARLNFSHGDHEEHGNRIDTIRKVEAELGKP
CCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHCCCHHHHHHHHHHHHCCC
IGIMLDTKGPEIRTGMLKDDKVELKKGEEIILTTEDIEGDENRVSVSYKGLPDDLHEGAT
EEEEEECCCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCHHHHCCCE
VLIDDGLIGLEVLEIKGTEIRCKIVNGGLLGSRKGVNLPGISVNLPALTEKDESDIRFGV
EEEECCCEEEEEEEEECCEEEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHH
RKGIHFIAASFVRKAQDVIEIRKILEEEGKEDILIIAKIENQEGVDNIDEIIDVADGIMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHCCEEE
ARGDLGVEIPAEQVPVIQKSIIKKCNEKAKPVITATQMLDSMIRNPRPTRAEASDVANAI
EECCCCCCCCHHHCCHHHHHHHHHHCHHCCCEEHHHHHHHHHHCCCCCCCCCHHHHHHHH
FDGTDATMLSGESAAGDYPVEAVKTMARIAEETEKSLYYRDVISNRRTYRPQTVTDAISF
HCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
ASCETATDLGAQAIITSTESGLTARMVSRYRPLVPIVAVTPDERVQHALTVSWGVYPLTV
HCCCCHHHCCCEEEEEECCCCHHHHHHHHCCCCEEEEEECCHHHHCEEEEEEECEEEEEE
KKSNSTDEMMDVSIKTAQENRLIKSGDLVVITAGAPVGIPGTTNLIKVDVVGEPLVEGQG
ECCCCCCHHEEEEEEECCCCCEEECCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCC
VGKGIVTGQVKIAHTAKEAVEKIEEGDILVTGITDKEYLPAMKKAGAIIAEQGGLTSHPA
CCCCEEEEEEEEEHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCEEEEECCCCCCCCE
IVGLELGIPVIVKAEKATENLVEGEIITIDGVRGLVYPGTVHLK
EEEEECCCCEEEEECHHHHHHHCCCEEEEECCCCEECCCEEEEC
>Mature Secondary Structure
MRKTKIVCTIGPVSESKEMLKKLAEAGMNVARLNFSHGDHEEHGNRIDTIRKVEAELGKP
CCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHCCCHHHHHHHHHHHHCCC
IGIMLDTKGPEIRTGMLKDDKVELKKGEEIILTTEDIEGDENRVSVSYKGLPDDLHEGAT
EEEEEECCCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCHHHHCCCE
VLIDDGLIGLEVLEIKGTEIRCKIVNGGLLGSRKGVNLPGISVNLPALTEKDESDIRFGV
EEEECCCEEEEEEEEECCEEEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHH
RKGIHFIAASFVRKAQDVIEIRKILEEEGKEDILIIAKIENQEGVDNIDEIIDVADGIMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHCCEEE
ARGDLGVEIPAEQVPVIQKSIIKKCNEKAKPVITATQMLDSMIRNPRPTRAEASDVANAI
EECCCCCCCCHHHCCHHHHHHHHHHCHHCCCEEHHHHHHHHHHCCCCCCCCCHHHHHHHH
FDGTDATMLSGESAAGDYPVEAVKTMARIAEETEKSLYYRDVISNRRTYRPQTVTDAISF
HCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
ASCETATDLGAQAIITSTESGLTARMVSRYRPLVPIVAVTPDERVQHALTVSWGVYPLTV
HCCCCHHHCCCEEEEEECCCCHHHHHHHHCCCCEEEEEECCHHHHCEEEEEEECEEEEEE
KKSNSTDEMMDVSIKTAQENRLIKSGDLVVITAGAPVGIPGTTNLIKVDVVGEPLVEGQG
ECCCCCCHHEEEEEEECCCCCEEECCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCC
VGKGIVTGQVKIAHTAKEAVEKIEEGDILVTGITDKEYLPAMKKAGAIIAEQGGLTSHPA
CCCCEEEEEEEEEHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCEEEEECCCCCCCCE
IVGLELGIPVIVKAEKATENLVEGEIITIDGVRGLVYPGTVHLK
EEEEECCCCEEEEECHHHHHHHCCCEEEEECCCCEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 9298659 [H]