Click here to switch to the map view.

The map label for this gene is pfkA

Identifier: 220931169

GI number: 220931169

Start: 329307

End: 330305

Strand: Direct

Name: pfkA

Synonym: Hore_03210

Alternate gene names: 220931169

Gene position: 329307-330305 (Clockwise)

Preceding gene: 220931168

Following gene: 220931170

Centisome position: 12.77

GC content: 41.54

Gene sequence:

>999_bases
ATGAAGAAAATAGGTGTATTAACAAGCGGTGGAGATGCCCCGGGAATGAATGCTGCCATCAGGTCTGTGGTTAGAACTGG
TATATATCACGGACTTGATGTTGTTGGGATAAGAAGGGGTTATGCCGGTTTAATCGAAGGTGATTTTTATTTAATGGACA
GGGGTTCTGTGGCCGATATTATTCAACGTGGGGGAACTATTCTCCTTTCAGCCCGTTGTGAAGAATTTAAAACAGAAGAA
GGCCGGCAAAAGGCCCTTAAAAAAATGAAAGAAGAAGGTATTGAAGGCCTGGTTGTTATTGGTGGTGACGGCTCCTTCAG
GGGAGCAGAAAAAGTAAGCAAAGAGCTGGGTATTCCTGCAATTGGAATTCCGGGTACTATAGATAATGATCTGGCCTGTA
CTGATTTTACTATTGGATTTGATACTGCCATGAATACTATTATCGATACTATCAATAAAATCAGGGATACGGCTACTTCC
CATGAAAGGACCTTTGTTGTAGAAACAATGGGACGCAGATCCGGGTTTTTAACCCTTATGGCCGGTCTAGCCGGTGGTGC
CGAATCCATTTTAATACCTGAAATTGACTTTGATCTGGATGATGTTTCCCAGAAATTAAAAAAAGGTTATGAACGTGGTA
AACTGCATAGTATTATTCTGGTAGCTGAAGGTGTTGGTGATGACTTCAGTACCAACCGTGATATAAACCACAGTAAGGCC
TTTGTTATTGGCAAGCAGATAGCTGAAAAAACGGGACTCGAAACAAGGGTCATTATCCTTGGTCATATTCAGAGGGGTGG
CTCTCCTACAGCTATGGACCGGATTCTGGCAAGTCGAATGGGGTCTAAAGCGGTAGAATTGCTCCTGGCTAGTGAATCTA
ATAAAATGGTAGGATATGTTAATAATAAACTTGTAACCTGTGATATTAGCGAAGCCCTCAGTAAGGAAAAACAGGTCGAA
AAAGATGTATATAAGCTAGCCAATATTCTTTCTATGTAA

Upstream 100 bases:

>100_bases
GAGAAGGTATCATAAATTCAGGAAAATGGGTATATATACCGTTACCAGTAAAACTAAAAATCTAAATAATGTTCAATCTA
ACTCATAGGGAGGTATTGGA

Downstream 100 bases:

>100_bases
TAATGGTTAATAAGTGTTTGTTTTTGAAAAACTAATATGTACTAACTGCCCTTTTTACCAGGTTTCCTACATAATTGTGT
TATAACCTGAATACCCGGAT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKKIGVLTSGGDAPGMNAAIRSVVRTGIYHGLDVVGIRRGYAGLIEGDFYLMDRGSVADIIQRGGTILLSARCEEFKTEE
GRQKALKKMKEEGIEGLVVIGGDGSFRGAEKVSKELGIPAIGIPGTIDNDLACTDFTIGFDTAMNTIIDTINKIRDTATS
HERTFVVETMGRRSGFLTLMAGLAGGAESILIPEIDFDLDDVSQKLKKGYERGKLHSIILVAEGVGDDFSTNRDINHSKA
FVIGKQIAEKTGLETRVIILGHIQRGGSPTAMDRILASRMGSKAVELLLASESNKMVGYVNNKLVTCDISEALSKEKQVE
KDVYKLANILSM

Sequences:

>Translated_332_residues
MKKIGVLTSGGDAPGMNAAIRSVVRTGIYHGLDVVGIRRGYAGLIEGDFYLMDRGSVADIIQRGGTILLSARCEEFKTEE
GRQKALKKMKEEGIEGLVVIGGDGSFRGAEKVSKELGIPAIGIPGTIDNDLACTDFTIGFDTAMNTIIDTINKIRDTATS
HERTFVVETMGRRSGFLTLMAGLAGGAESILIPEIDFDLDDVSQKLKKGYERGKLHSIILVAEGVGDDFSTNRDINHSKA
FVIGKQIAEKTGLETRVIILGHIQRGGSPTAMDRILASRMGSKAVELLLASESNKMVGYVNNKLVTCDISEALSKEKQVE
KDVYKLANILSM
>Mature_332_residues
MKKIGVLTSGGDAPGMNAAIRSVVRTGIYHGLDVVGIRRGYAGLIEGDFYLMDRGSVADIIQRGGTILLSARCEEFKTEE
GRQKALKKMKEEGIEGLVVIGGDGSFRGAEKVSKELGIPAIGIPGTIDNDLACTDFTIGFDTAMNTIIDTINKIRDTATS
HERTFVVETMGRRSGFLTLMAGLAGGAESILIPEIDFDLDDVSQKLKKGYERGKLHSIILVAEGVGDDFSTNRDINHSKA
FVIGKQIAEKTGLETRVIILGHIQRGGSPTAMDRILASRMGSKAVELLLASESNKMVGYVNNKLVTCDISEALSKEKQVE
KDVYKLANILSM

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI11321601, Length=350, Percent_Identity=42.8571428571429, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI48762920, Length=350, Percent_Identity=41.4285714285714, Blast_Score=237, Evalue=9e-63,
Organism=Homo sapiens, GI266453619, Length=357, Percent_Identity=40.0560224089636, Blast_Score=229, Evalue=2e-60,
Organism=Homo sapiens, GI266453768, Length=357, Percent_Identity=40.0560224089636, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI266453748, Length=357, Percent_Identity=40.0560224089636, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI4505749, Length=357, Percent_Identity=40.0560224089636, Blast_Score=229, Evalue=3e-60,
Organism=Escherichia coli, GI1790350, Length=321, Percent_Identity=50.4672897196262, Blast_Score=306, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI25147584, Length=350, Percent_Identity=40.2857142857143, Blast_Score=221, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI17558788, Length=317, Percent_Identity=39.1167192429022, Blast_Score=211, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI72003601, Length=297, Percent_Identity=32.996632996633, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6321679, Length=349, Percent_Identity=39.2550143266476, Blast_Score=223, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6323861, Length=360, Percent_Identity=36.1111111111111, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24652337, Length=351, Percent_Identity=39.031339031339, Blast_Score=228, Evalue=3e-60,
Organism=Drosophila melanogaster, GI17647809, Length=351, Percent_Identity=39.031339031339, Blast_Score=228, Evalue=3e-60,
Organism=Drosophila melanogaster, GI28573326, Length=350, Percent_Identity=38.2857142857143, Blast_Score=225, Evalue=3e-59,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF_HALOH (B8D1K5)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002508077.1
- ProteinModelPortal:   B8D1K5
- GeneID:   7314707
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_03210
- HOGENOM:   HBG405044
- OMA:   RCEEFKT
- ProtClustDB:   CLSK2806201
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476
- TIGRFAMs:   TIGR02482

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 35771; Mature: 35771

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 128-128 BINDING 163-163 BINDING 256-256 BINDING 262-262 BINDING 265-265

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIGVLTSGGDAPGMNAAIRSVVRTGIYHGLDVVGIRRGYAGLIEGDFYLMDRGSVADI
CCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCEEEEECCCHHHH
IQRGGTILLSARCEEFKTEEGRQKALKKMKEEGIEGLVVIGGDGSFRGAEKVSKELGIPA
HHCCCEEEEECCHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCC
IGIPGTIDNDLACTDFTIGFDTAMNTIIDTINKIRDTATSHERTFVVETMGRRSGFLTLM
CCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHH
AGLAGGAESILIPEIDFDLDDVSQKLKKGYERGKLHSIILVAEGVGDDFSTNRDINHSKA
HHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCHH
FVIGKQIAEKTGLETRVIILGHIQRGGSPTAMDRILASRMGSKAVELLLASESNKMVGYV
HHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCHHHHHEEEECCCCCEEEEE
NNKLVTCDISEALSKEKQVEKDVYKLANILSM
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKIGVLTSGGDAPGMNAAIRSVVRTGIYHGLDVVGIRRGYAGLIEGDFYLMDRGSVADI
CCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCEEEEECCCHHHH
IQRGGTILLSARCEEFKTEEGRQKALKKMKEEGIEGLVVIGGDGSFRGAEKVSKELGIPA
HHCCCEEEEECCHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCC
IGIPGTIDNDLACTDFTIGFDTAMNTIIDTINKIRDTATSHERTFVVETMGRRSGFLTLM
CCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHH
AGLAGGAESILIPEIDFDLDDVSQKLKKGYERGKLHSIILVAEGVGDDFSTNRDINHSKA
HHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCHH
FVIGKQIAEKTGLETRVIILGHIQRGGSPTAMDRILASRMGSKAVELLLASESNKMVGYV
HHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCHHHHHEEEECCCCCEEEEE
NNKLVTCDISEALSKEKQVEKDVYKLANILSM
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA