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The map label for this gene is ligA

Identifier: 220931078

GI number: 220931078

Start: 236182

End: 238194

Strand: Direct

Name: ligA

Synonym: Hore_02300

Alternate gene names: 220931078

Gene position: 236182-238194 (Clockwise)

Preceding gene: 220931077

Following gene: 220931079

Centisome position: 9.16

GC content: 44.86

Gene sequence:

>2013_bases
ATGGCCAGTAAAGTAGAACAGGAAATAAGGGATTTAAGGGAGAAGATACGGTATCATGAGTACCGGTATTATGTTCTAGA
TGACCCGGAAATATCTGATGCTGAATTTGATGAATTGATACAGAGGCTTATTGATCTGGAGGAAAAGCACCCTGGCCTGG
TGACCCCGGATTCTCCTACTCAACGGGTCGGTGGAGAGCCTCTTGATAAATTTGATAAAGTAGAGCACCGGGTTCCCATG
CTGAGCCTTGGTAATGCATTTAATGAGGGTGATTTAACGAATTTTGCCAGGCGGATTTACCGGCTCCTTGATACAGGCAA
AATAGACTTCGTGGTGGAACATAAAATCGATGGGTTATCAGCGATATTAACCTATCAGGGTGGTAGGTTGATCCGGGGAG
CAACCCGCGGTAATGGAGTGGTGGGGGAAGATGTTACCGCCAATATAAAGACCATCCCTTCTGTACCCCTCAGGTTAAAA
AAAGATGTTGATATCGAAGTCCGGGGTGAGGTATATATAAAAAAAGATGATTTTAGTAAATTAAATGAAAGGAGATTGAA
GAAGGGAGAAGAACCCTTTGCTAATCCCCGGAATGCAGCGGCAGGTTCAATTAGACAGCTTGACCCCAGGCTGGCTGCCG
CCCGTCCCCTGTCCTTTATTGCCTACGATGTAGTTGCCTATGAAGGAGAAGGTTTACAGACCCATGTCGGGGCCCTGGAA
TTACTCCGGGAGCTTGGTTTTAAGGTGAACTGGTATCGTAAATGTGATGATATTACAGAGGTAGTAAATATTTGTAAGGA
CTGGGTGGATAAAAGAGAAGAATTACCCTTTGAGATAGATGGTATGGTTATTAAGGTCAATGAACTGGGTCTGAGAGAAC
AGCTGGGAGCTACTGCCAAAAGCCCCCGCTGGGCCATTGCTTATAAATTCCCTGCCCAGCAGAAGACAACTGTTGTTAAA
GATATTATTATTTCGGTAGGCCGGACCGGGGCCTTAACACCGACAGCTGTCCTGGAGCCTGTTGAGGTTGATGGTTCTAC
AGTCAGCAGGGCAACTCTCCATAATGAAGATGAAATAAGGAGAAAAGATGTTAGAATAGGGGATCATGTCCTGGTTCAAA
AAGCAGGTGATGTCATCCCTGAAGTGGTTAAGGTTATAAAGAGTAAACGGGATGGCAGTGAACAGATATTTCACATGCCC
GAAACCTGCCCTGCCTGTGGTGGGGAGGTTGTGAGGGAAGAAGGTGAAGCTGTTTTAAGGTGTGTTAATGTGACCGGGTG
CCCTGCCCAGAGACGGGAAGGAATTCTTCACTTTGTCTCCCGGAATGCCATGAATATTGATGGCGTGGGGCCAGCCCTGA
TAGACCAGCTTTTAGAAAAAGGGTTGATAGAAGATTATGCTGATTTATATTACCTTAAAAAAGAGGATTTGATTCCTCTG
GAAAGAATGGGGGAAAAATCAGCTACTAATGCCATTGAGGCTATCAGGGCCAGTAAGGACAGGCCTCTCTTTAGAGTTAT
TTTTGCCCTGGGGATCAGGCATGTCGGTCTGGGGGTAGCCCGGGTTTTAACTGAAAAATACCGGTCATTGTCTGATTTAA
TGAGGGCATCCGGGGAAGAACTGGTGGCTATTGATGAAATTGGTCCTACTATAGCCCGGAGTATTATTGAATTTTTTAAG
GAGCCCCATAACCGGGAGGTTATTAATAAATTAAAAGAAGCCGGGGTGAGACTTGAGGAGAAGGAAAATGGAAAAGAAGA
GCAGAATTTATACCTGTCAGGAAAAACATTTGTCTTTACCGGTAAATTAGATGGTTTTACCCGGAGTGAAGCCAGAGATA
AGGTTATAGCAGCAGGAGGAAAGGTTACTTCAAGTGTCAGCCGGAAAACCGACTATGTAGTGGTCGGAGATAGTCCCGGT
TCCAAATATGATAAAGCCCGGGAGTTGGGGGTAACTATTTTAGATGAAGATAAATTTAAAGAAGTGTTAAAAGCGGGGGA
TAATAATGGTTAG

Upstream 100 bases:

>100_bases
TGAAAACTCTTCTGGCCGAGTATGCCCCCATTCAGAAGGCTTAATCACCGGAAAACTTAACCACTAGAAGACTTAACCAT
GAGGAAAGGCAGGTGGGATT

Downstream 100 bases:

>100_bases
GTTATCACTGGGTATATCTCTGGTTATGGTTGTCATTCTTCTGGCAACGAAAAGTTCTCATTTGTGGCCGGGTACTTTTT
TCTTCCTGGTTCAATTGGTA

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 670; Mature: 669

Protein sequence:

>670_residues
MASKVEQEIRDLREKIRYHEYRYYVLDDPEISDAEFDELIQRLIDLEEKHPGLVTPDSPTQRVGGEPLDKFDKVEHRVPM
LSLGNAFNEGDLTNFARRIYRLLDTGKIDFVVEHKIDGLSAILTYQGGRLIRGATRGNGVVGEDVTANIKTIPSVPLRLK
KDVDIEVRGEVYIKKDDFSKLNERRLKKGEEPFANPRNAAAGSIRQLDPRLAAARPLSFIAYDVVAYEGEGLQTHVGALE
LLRELGFKVNWYRKCDDITEVVNICKDWVDKREELPFEIDGMVIKVNELGLREQLGATAKSPRWAIAYKFPAQQKTTVVK
DIIISVGRTGALTPTAVLEPVEVDGSTVSRATLHNEDEIRRKDVRIGDHVLVQKAGDVIPEVVKVIKSKRDGSEQIFHMP
ETCPACGGEVVREEGEAVLRCVNVTGCPAQRREGILHFVSRNAMNIDGVGPALIDQLLEKGLIEDYADLYYLKKEDLIPL
ERMGEKSATNAIEAIRASKDRPLFRVIFALGIRHVGLGVARVLTEKYRSLSDLMRASGEELVAIDEIGPTIARSIIEFFK
EPHNREVINKLKEAGVRLEEKENGKEEQNLYLSGKTFVFTGKLDGFTRSEARDKVIAAGGKVTSSVSRKTDYVVVGDSPG
SKYDKARELGVTILDEDKFKEVLKAGDNNG

Sequences:

>Translated_670_residues
MASKVEQEIRDLREKIRYHEYRYYVLDDPEISDAEFDELIQRLIDLEEKHPGLVTPDSPTQRVGGEPLDKFDKVEHRVPM
LSLGNAFNEGDLTNFARRIYRLLDTGKIDFVVEHKIDGLSAILTYQGGRLIRGATRGNGVVGEDVTANIKTIPSVPLRLK
KDVDIEVRGEVYIKKDDFSKLNERRLKKGEEPFANPRNAAAGSIRQLDPRLAAARPLSFIAYDVVAYEGEGLQTHVGALE
LLRELGFKVNWYRKCDDITEVVNICKDWVDKREELPFEIDGMVIKVNELGLREQLGATAKSPRWAIAYKFPAQQKTTVVK
DIIISVGRTGALTPTAVLEPVEVDGSTVSRATLHNEDEIRRKDVRIGDHVLVQKAGDVIPEVVKVIKSKRDGSEQIFHMP
ETCPACGGEVVREEGEAVLRCVNVTGCPAQRREGILHFVSRNAMNIDGVGPALIDQLLEKGLIEDYADLYYLKKEDLIPL
ERMGEKSATNAIEAIRASKDRPLFRVIFALGIRHVGLGVARVLTEKYRSLSDLMRASGEELVAIDEIGPTIARSIIEFFK
EPHNREVINKLKEAGVRLEEKENGKEEQNLYLSGKTFVFTGKLDGFTRSEARDKVIAAGGKVTSSVSRKTDYVVVGDSPG
SKYDKARELGVTILDEDKFKEVLKAGDNNG
>Mature_669_residues
ASKVEQEIRDLREKIRYHEYRYYVLDDPEISDAEFDELIQRLIDLEEKHPGLVTPDSPTQRVGGEPLDKFDKVEHRVPML
SLGNAFNEGDLTNFARRIYRLLDTGKIDFVVEHKIDGLSAILTYQGGRLIRGATRGNGVVGEDVTANIKTIPSVPLRLKK
DVDIEVRGEVYIKKDDFSKLNERRLKKGEEPFANPRNAAAGSIRQLDPRLAAARPLSFIAYDVVAYEGEGLQTHVGALEL
LRELGFKVNWYRKCDDITEVVNICKDWVDKREELPFEIDGMVIKVNELGLREQLGATAKSPRWAIAYKFPAQQKTTVVKD
IIISVGRTGALTPTAVLEPVEVDGSTVSRATLHNEDEIRRKDVRIGDHVLVQKAGDVIPEVVKVIKSKRDGSEQIFHMPE
TCPACGGEVVREEGEAVLRCVNVTGCPAQRREGILHFVSRNAMNIDGVGPALIDQLLEKGLIEDYADLYYLKKEDLIPLE
RMGEKSATNAIEAIRASKDRPLFRVIFALGIRHVGLGVARVLTEKYRSLSDLMRASGEELVAIDEIGPTIARSIIEFFKE
PHNREVINKLKEAGVRLEEKENGKEEQNLYLSGKTFVFTGKLDGFTRSEARDKVIAAGGKVTSSVSRKTDYVVVGDSPGS
KYDKARELGVTILDEDKFKEVLKAGDNNG

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=669, Percent_Identity=46.6367713004484, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI87082305, Length=567, Percent_Identity=23.2804232804233, Blast_Score=119, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_HALOH (B8D122)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002507986.1
- ProteinModelPortal:   B8D122
- GeneID:   7312550
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_02300
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74873; Mature: 74742

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 115-115 BINDING 113-113 BINDING 136-136 BINDING 170-170 BINDING 285-285 BINDING 309-309

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKVEQEIRDLREKIRYHEYRYYVLDDPEISDAEFDELIQRLIDLEEKHPGLVTPDSPT
CCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCH
QRVGGEPLDKFDKVEHRVPMLSLGNAFNEGDLTNFARRIYRLLDTGKIDFVVEHKIDGLS
HHCCCCCHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCE
AILTYQGGRLIRGATRGNGVVGEDVTANIKTIPSVPLRLKKDVDIEVRGEVYIKKDDFSK
EEEEECCCEEEEECCCCCCCCCCCCCCCCEECCCCCCEECCCCCEEECCEEEEECCCHHH
LNERRLKKGEEPFANPRNAAAGSIRQLDPRLAAARPLSFIAYDVVAYEGEGLQTHVGALE
HHHHHHHCCCCCCCCCCCCCCCCHHHCCCHHHHHCCHHHHHHHHEEECCCCCHHHHHHHH
LLRELGFKVNWYRKCDDITEVVNICKDWVDKREELPFEIDGMVIKVNELGLREQLGATAK
HHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEEECCCCHHHHCCCCC
SPRWAIAYKFPAQQKTTVVKDIIISVGRTGALTPTAVLEPVEVDGSTVSRATLHNEDEIR
CCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCEECCCCCCHHHHCCCHHHHH
RKDVRIGDHVLVQKAGDVIPEVVKVIKSKRDGSEQIFHMPETCPACGGEVVREEGEAVLR
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHEECCCCCCCCCHHHHHHHCHHHEE
CVNVTGCPAQRREGILHFVSRNAMNIDGVGPALIDQLLEKGLIEDYADLYYLKKEDLIPL
EECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCH
ERMGEKSATNAIEAIRASKDRPLFRVIFALGIRHVGLGVARVLTEKYRSLSDLMRASGEE
HHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LVAIDEIGPTIARSIIEFFKEPHNREVINKLKEAGVRLEEKENGKEEQNLYLSGKTFVFT
EEEEHHCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEHHCCCCCCCCEEECCCEEEEE
GKLDGFTRSEARDKVIAAGGKVTSSVSRKTDYVVVGDSPGSKYDKARELGVTILDEDKFK
ECCCCCCHHHHHCCEEECCCCHHHHHHCCCCEEEEECCCCCHHHHHHHCCCEEECHHHHH
EVLKAGDNNG
HHHHCCCCCC
>Mature Secondary Structure 
ASKVEQEIRDLREKIRYHEYRYYVLDDPEISDAEFDELIQRLIDLEEKHPGLVTPDSPT
CHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCH
QRVGGEPLDKFDKVEHRVPMLSLGNAFNEGDLTNFARRIYRLLDTGKIDFVVEHKIDGLS
HHCCCCCHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCE
AILTYQGGRLIRGATRGNGVVGEDVTANIKTIPSVPLRLKKDVDIEVRGEVYIKKDDFSK
EEEEECCCEEEEECCCCCCCCCCCCCCCCEECCCCCCEECCCCCEEECCEEEEECCCHHH
LNERRLKKGEEPFANPRNAAAGSIRQLDPRLAAARPLSFIAYDVVAYEGEGLQTHVGALE
HHHHHHHCCCCCCCCCCCCCCCCHHHCCCHHHHHCCHHHHHHHHEEECCCCCHHHHHHHH
LLRELGFKVNWYRKCDDITEVVNICKDWVDKREELPFEIDGMVIKVNELGLREQLGATAK
HHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEEECCCCHHHHCCCCC
SPRWAIAYKFPAQQKTTVVKDIIISVGRTGALTPTAVLEPVEVDGSTVSRATLHNEDEIR
CCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCEECCCCCCHHHHCCCHHHHH
RKDVRIGDHVLVQKAGDVIPEVVKVIKSKRDGSEQIFHMPETCPACGGEVVREEGEAVLR
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHEECCCCCCCCCHHHHHHHCHHHEE
CVNVTGCPAQRREGILHFVSRNAMNIDGVGPALIDQLLEKGLIEDYADLYYLKKEDLIPL
EECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCH
ERMGEKSATNAIEAIRASKDRPLFRVIFALGIRHVGLGVARVLTEKYRSLSDLMRASGEE
HHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LVAIDEIGPTIARSIIEFFKEPHNREVINKLKEAGVRLEEKENGKEEQNLYLSGKTFVFT
EEEEHHCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEHHCCCCCCCCEEECCCEEEEE
GKLDGFTRSEARDKVIAAGGKVTSSVSRKTDYVVVGDSPGSKYDKARELGVTILDEDKFK
ECCCCCCHHHHHCCEEECCCCHHHHHHCCCCEEEEECCCCCHHHHHHHCCCEEECHHHHH
EVLKAGDNNG
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA