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The map label for this gene is pcrA [H]

Identifier: 220931077

GI number: 220931077

Start: 233978

End: 236125

Strand: Direct

Name: pcrA [H]

Synonym: Hore_02290

Alternate gene names: 220931077

Gene position: 233978-236125 (Clockwise)

Preceding gene: 220931076

Following gene: 220931078

Centisome position: 9.08

GC content: 42.18

Gene sequence:

>2148_bases
TTGTTGTCTGATAGCAGTGATATTCTGACAGGATTAAATCCTGAACAAAAGAAAGCTGTAGAACATTTTGAAGGCCCCCT
GTTGATTTTAGCGGGGGCCGGTAGTGGGAAAACCAGGGTGTTAACCCACCGCATTGCATATCTAATTGAAAATTATGGTG
TTAATCCCCTTCAGATACTGGCAGTAACCTTTACCAATAAGGCTGCCGGTGAGATGAAGGAGAGGGTTGATAATCTTCTG
GGAGGAATGGCTGGAGACCTCTGGGTCAGCACCTTTCATTCATTATGTGCCCGTATTTTACGGAAAGAGATAGGTAAGAT
CGGTTATGATAATAACTTTGTAATCTTCGATACCGATGACCAGCAGAAATTAATATCCAGGATTTTAAAAGAATTAAATC
TCGATCCGAAAAAAACCAGGCCAAGGGCTATCTTATCTGAAATTAGCAGGGCCAAAAATGAGTTAATTGATCCCCGGTCC
TATGCCAATAATGTTGGTGATTACTTTCAGGATATAACAGCCCGTATTTATCCCCTGTACCAGGAAAGGCTTAAGGAGAG
CAACGCTCTTGATTTTGACGATTTAATTATGAAAACCATTGAGGTTTTTGTGGACAACCCCATGGTTTTAGAGTATTACC
AGGAACGATTCAAGTATATCCTTGTTGACGAGTACCAGGATGTTAACTTTGCCCAGTATAAACTGGTTCAACTCCTGGCC
AATAAATACCGGAATCTCTGTGTTGTCGGTGACCCCGACCAGGGAATATACGGTTTCCGGGGGGCTGATATTCGTAATAT
CCTTAATTTTGAGGAAGATTACCCGGAGGCCAGGGTTATAAAGCTGGAACAGAACTACCGCTCCAAAGAGAAAATATTAA
AGGCTGCCCATCATGTCATCCGCAATAATACAGCCCGTAAGGAAAAGCGTCTCTGGACAAAACGGGGTAAAGGTGAAGAT
TTGAAGCTTTATGTGGCTTTTGATGACAAAGATGAGGCTTCCTATGTCTGCCGGAAAATAAAAGAATTAAAGAGGGAGAA
AAATTATAAATTTAGTGATTTTGCTGTGCTCTACCGTACCAATTCCCAGTCCCGGTCTGTAGAAGAGATGATGGTTAAGT
ATGCCATACCTTACCAGATTGTTGGTGGATTTCGGTTTTATGACCGGATGGAAATTAAAGATATTCTGGCCTATCTCAGG
GTTATCTATAATCCATCCGATGAAGTCAGTTTATTGCGGATTATTAATCGTCCTAAAAGGGGCATAGGGCAGGGAACCAT
TAGCAAGTTATCAAGATATGCCAGAGAGAGGGGGATAAGCCTGTATAAGGCTGGAACAGAGGCAGAATCTAACCCTTATC
TTACCGCTTCTTTTAAAAAGAGGGTTAAAGCCTTTTTTGACCTTCTTGAGGAACTGAGGGAAAAAAGTGAAACCTTAAGT
ATTGATACGTTAACCCATCAGGTAGTGACCAGAACGGGGTACCAGCGTGAACTTAATGAAGAAGGTACCCAGCAGGCCCG
AAACCGGCTGGAAAATATCCAGGAGTTATTTTCAGTGATTGAGGAGTTTATGAAGGGTAATGAAAATAAAACTCTGGGTG
CTTTCCTGGAAGAAGTATCACTCATTTCTGATGTAGATAATATGGAGGATAATCAGAATGTGGTTACCCTGATGACACTC
CATTCTGCCAAAGGGCTGGAATTTCCAGTTGTTTTTATTATCGGAATGGAAGAGGGTTTATTCCCTCACGCCAACTCCAT
GATGGATCATGAAGAACTGGAAGAAGAGAGGCGATTATGTTATGTTGGTATAACCAGGGCCAGGGACGAACTATATTTGA
CTAGGGCCCGGGAGCGGATGAGGTTTGGTCAACGGAAACCCTACCCCCCTTCCAGGTTTTTAGATGAAATTCCTCCACAG
TTATTTGAGGATAATAATGATAAAAAAGAGGAAATATTGGGTAATATAAAAGAGAAAGAAAGTAAAACCGGAGAATATAA
AGTAGGAGATACAGTGGTTCACCCCAGGTGGGGAAAAGGACAGATTGTAGGTGTCAGGGAAAACAGGGGGCTTGAGCTTA
AGATAAACTTCGGAAAAGGGAAGGTGAAAACTCTTCTGGCCGAGTATGCCCCCATTCAGAAGGCTTAA

Upstream 100 bases:

>100_bases
TTTTGAATACTGATATAAGAATAATTTATAGAATGATATAAAAAAGAAGGATATATTAATATATTTATAAATAAAATAGA
CTTAGTAACAGGGAGTGGTA

Downstream 100 bases:

>100_bases
TCACCGGAAAACTTAACCACTAGAAGACTTAACCATGAGGAAAGGCAGGTGGGATTATGGCCAGTAAAGTAGAACAGGAA
ATAAGGGATTTAAGGGAGAA

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 715; Mature: 715

Protein sequence:

>715_residues
MLSDSSDILTGLNPEQKKAVEHFEGPLLILAGAGSGKTRVLTHRIAYLIENYGVNPLQILAVTFTNKAAGEMKERVDNLL
GGMAGDLWVSTFHSLCARILRKEIGKIGYDNNFVIFDTDDQQKLISRILKELNLDPKKTRPRAILSEISRAKNELIDPRS
YANNVGDYFQDITARIYPLYQERLKESNALDFDDLIMKTIEVFVDNPMVLEYYQERFKYILVDEYQDVNFAQYKLVQLLA
NKYRNLCVVGDPDQGIYGFRGADIRNILNFEEDYPEARVIKLEQNYRSKEKILKAAHHVIRNNTARKEKRLWTKRGKGED
LKLYVAFDDKDEASYVCRKIKELKREKNYKFSDFAVLYRTNSQSRSVEEMMVKYAIPYQIVGGFRFYDRMEIKDILAYLR
VIYNPSDEVSLLRIINRPKRGIGQGTISKLSRYARERGISLYKAGTEAESNPYLTASFKKRVKAFFDLLEELREKSETLS
IDTLTHQVVTRTGYQRELNEEGTQQARNRLENIQELFSVIEEFMKGNENKTLGAFLEEVSLISDVDNMEDNQNVVTLMTL
HSAKGLEFPVVFIIGMEEGLFPHANSMMDHEELEEERRLCYVGITRARDELYLTRARERMRFGQRKPYPPSRFLDEIPPQ
LFEDNNDKKEEILGNIKEKESKTGEYKVGDTVVHPRWGKGQIVGVRENRGLELKINFGKGKVKTLLAEYAPIQKA

Sequences:

>Translated_715_residues
MLSDSSDILTGLNPEQKKAVEHFEGPLLILAGAGSGKTRVLTHRIAYLIENYGVNPLQILAVTFTNKAAGEMKERVDNLL
GGMAGDLWVSTFHSLCARILRKEIGKIGYDNNFVIFDTDDQQKLISRILKELNLDPKKTRPRAILSEISRAKNELIDPRS
YANNVGDYFQDITARIYPLYQERLKESNALDFDDLIMKTIEVFVDNPMVLEYYQERFKYILVDEYQDVNFAQYKLVQLLA
NKYRNLCVVGDPDQGIYGFRGADIRNILNFEEDYPEARVIKLEQNYRSKEKILKAAHHVIRNNTARKEKRLWTKRGKGED
LKLYVAFDDKDEASYVCRKIKELKREKNYKFSDFAVLYRTNSQSRSVEEMMVKYAIPYQIVGGFRFYDRMEIKDILAYLR
VIYNPSDEVSLLRIINRPKRGIGQGTISKLSRYARERGISLYKAGTEAESNPYLTASFKKRVKAFFDLLEELREKSETLS
IDTLTHQVVTRTGYQRELNEEGTQQARNRLENIQELFSVIEEFMKGNENKTLGAFLEEVSLISDVDNMEDNQNVVTLMTL
HSAKGLEFPVVFIIGMEEGLFPHANSMMDHEELEEERRLCYVGITRARDELYLTRARERMRFGQRKPYPPSRFLDEIPPQ
LFEDNNDKKEEILGNIKEKESKTGEYKVGDTVVHPRWGKGQIVGVRENRGLELKINFGKGKVKTLLAEYAPIQKA
>Mature_715_residues
MLSDSSDILTGLNPEQKKAVEHFEGPLLILAGAGSGKTRVLTHRIAYLIENYGVNPLQILAVTFTNKAAGEMKERVDNLL
GGMAGDLWVSTFHSLCARILRKEIGKIGYDNNFVIFDTDDQQKLISRILKELNLDPKKTRPRAILSEISRAKNELIDPRS
YANNVGDYFQDITARIYPLYQERLKESNALDFDDLIMKTIEVFVDNPMVLEYYQERFKYILVDEYQDVNFAQYKLVQLLA
NKYRNLCVVGDPDQGIYGFRGADIRNILNFEEDYPEARVIKLEQNYRSKEKILKAAHHVIRNNTARKEKRLWTKRGKGED
LKLYVAFDDKDEASYVCRKIKELKREKNYKFSDFAVLYRTNSQSRSVEEMMVKYAIPYQIVGGFRFYDRMEIKDILAYLR
VIYNPSDEVSLLRIINRPKRGIGQGTISKLSRYARERGISLYKAGTEAESNPYLTASFKKRVKAFFDLLEELREKSETLS
IDTLTHQVVTRTGYQRELNEEGTQQARNRLENIQELFSVIEEFMKGNENKTLGAFLEEVSLISDVDNMEDNQNVVTLMTL
HSAKGLEFPVVFIIGMEEGLFPHANSMMDHEELEEERRLCYVGITRARDELYLTRARERMRFGQRKPYPPSRFLDEIPPQ
LFEDNNDKKEEILGNIKEKESKTGEYKVGDTVVHPRWGKGQIVGVRENRGLELKINFGKGKVKTLLAEYAPIQKA

Specific function: DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substra

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=726, Percent_Identity=39.5316804407713, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI48994965, Length=635, Percent_Identity=41.7322834645669, Blast_Score=450, Evalue=1e-127,
Organism=Escherichia coli, GI1787196, Length=356, Percent_Identity=29.2134831460674, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6322369, Length=459, Percent_Identity=33.1154684095861, Blast_Score=216, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6324477, Length=689, Percent_Identity=25.544267053701, Blast_Score=152, Evalue=3e-37,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 82748; Mature: 82748

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSDSSDILTGLNPEQKKAVEHFEGPLLILAGAGSGKTRVLTHRIAYLIENYGVNPLQIL
CCCCCCCHHCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEE
AVTFTNKAAGEMKERVDNLLGGMAGDLWVSTFHSLCARILRKEIGKIGYDNNFVIFDTDD
EEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
QQKLISRILKELNLDPKKTRPRAILSEISRAKNELIDPRSYANNVGDYFQDITARIYPLY
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
QERLKESNALDFDDLIMKTIEVFVDNPMVLEYYQERFKYILVDEYQDVNFAQYKLVQLLA
HHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHH
NKYRNLCVVGDPDQGIYGFRGADIRNILNFEEDYPEARVIKLEQNYRSKEKILKAAHHVI
HHCCCEEEECCCCCCCCCCCCCCHHHHHCCHHCCCCCEEEEEHHHCCHHHHHHHHHHHHH
RNNTARKEKRLWTKRGKGEDLKLYVAFDDKDEASYVCRKIKELKREKNYKFSDFAVLYRT
HCCCHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
NSQSRSVEEMMVKYAIPYQIVGGFRFYDRMEIKDILAYLRVIYNPSDEVSLLRIINRPKR
CCCCHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
GIGQGTISKLSRYARERGISLYKAGTEAESNPYLTASFKKRVKAFFDLLEELREKSETLS
CCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCEE
IDTLTHQVVTRTGYQRELNEEGTQQARNRLENIQELFSVIEEFMKGNENKTLGAFLEEVS
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LISDVDNMEDNQNVVTLMTLHSAKGLEFPVVFIIGMEEGLFPHANSMMDHEELEEERRLC
HHHHHHCCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCEE
YVGITRARDELYLTRARERMRFGQRKPYPPSRFLDEIPPQLFEDNNDKKEEILGNIKEKE
EEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHCCCCCHHHHHHCCHHHHH
SKTGEYKVGDTVVHPRWGKGQIVGVRENRGLELKINFGKGKVKTLLAEYAPIQKA
CCCCCEECCCEEECCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLSDSSDILTGLNPEQKKAVEHFEGPLLILAGAGSGKTRVLTHRIAYLIENYGVNPLQIL
CCCCCCCHHCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEE
AVTFTNKAAGEMKERVDNLLGGMAGDLWVSTFHSLCARILRKEIGKIGYDNNFVIFDTDD
EEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
QQKLISRILKELNLDPKKTRPRAILSEISRAKNELIDPRSYANNVGDYFQDITARIYPLY
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
QERLKESNALDFDDLIMKTIEVFVDNPMVLEYYQERFKYILVDEYQDVNFAQYKLVQLLA
HHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHH
NKYRNLCVVGDPDQGIYGFRGADIRNILNFEEDYPEARVIKLEQNYRSKEKILKAAHHVI
HHCCCEEEECCCCCCCCCCCCCCHHHHHCCHHCCCCCEEEEEHHHCCHHHHHHHHHHHHH
RNNTARKEKRLWTKRGKGEDLKLYVAFDDKDEASYVCRKIKELKREKNYKFSDFAVLYRT
HCCCHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
NSQSRSVEEMMVKYAIPYQIVGGFRFYDRMEIKDILAYLRVIYNPSDEVSLLRIINRPKR
CCCCHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
GIGQGTISKLSRYARERGISLYKAGTEAESNPYLTASFKKRVKAFFDLLEELREKSETLS
CCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCEE
IDTLTHQVVTRTGYQRELNEEGTQQARNRLENIQELFSVIEEFMKGNENKTLGAFLEEVS
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LISDVDNMEDNQNVVTLMTLHSAKGLEFPVVFIIGMEEGLFPHANSMMDHEELEEERRLC
HHHHHHCCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCEE
YVGITRARDELYLTRARERMRFGQRKPYPPSRFLDEIPPQLFEDNNDKKEEILGNIKEKE
EEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHCCCCCHHHHHHCCHHHHH
SKTGEYKVGDTVVHPRWGKGQIVGVRENRGLELKINFGKGKVKTLLAEYAPIQKA
CCCCCEECCCEEECCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9592155; 8934527; 10199404; 10388562; 10454638 [H]