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The map label for this gene is hisA

Identifier: 220931071

GI number: 220931071

Start: 230270

End: 230983

Strand: Direct

Name: hisA

Synonym: Hore_02230

Alternate gene names: 220931071

Gene position: 230270-230983 (Clockwise)

Preceding gene: 220931070

Following gene: 220931072

Centisome position: 8.93

GC content: 43.56

Gene sequence:

>714_bases
GTGGAAGTAATACCGGCAGTTGATATAAAAGATGGTAGTTGTGTCCGTCTTAAAAAAGGTGACTTTAATAAAAGGCGGGT
CTACAGTACCAGTCCAGTAGATGTGGCTCTGTACTGGGAAAAGCACGGGGCTTCCCGTCTCCATATTGTTGACCTTGATG
GGGCTAAATCGGGCTGGCCCACACACCTTAAGACAATTAGAGAAATTGCTTTAAGGGTTAATATACCGCTGCAGGTTGGA
GGGGGTATCAGGTCCCTTAAGGTAATAAAAAAATACCTGGATTCCGGTGTTGACAGAATTATCCTGGGGACGGTGGCCCT
TAAAAACCCGGAGCTGGTTAAAAGGGCCCTTGATAATTTTGGTTCAAACAGGATCGTGGTCGGGGTTGATGCCAGAGGTG
GTAAAGTAGCTACAGAAGGCTGGCTTAAAACCAGTCAGGTTACTGTAGAGGATATAATATCTGAGATGGAAGAAGTGGGA
GTAAAAACCTTTATTTATACCGATATCAACAGGGATGGTATGTTAAAAGGCCCCGATATTGAAGGAATAAAAAGGGTGTT
AAAATCAACTAAAGCCAGAATTATAGCTTCTGGAGGTATTTCGTCTCGCCAGGACTTAATTAACCTGAAGGCCATAGGTA
TTAAAGCGGCTATTGTAGGGAAGGCCCTTTATGAAGGCAATTTGCCTCTGGAAGTGTTAAATCAATATCCGTGA

Upstream 100 bases:

>100_bases
TTGTCAGGTCAAACAATGCCTGGGAAATACAGGGTCACCCAGAAAAGAGTAGCCGGACGGGTTTGAAGATTCTTCAAAAT
TTCAGTGAGGTGGTTAACAG

Downstream 100 bases:

>100_bases
AGTGTATACATATATAAGAAAATGGGGGAGAAGGTATGTTAACAAAAAGAATCATACCCTGTCTTGATGTTAAGGGTGGA
AGGGTTGTTAAGGGGGTAAA

Product: phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MEVIPAVDIKDGSCVRLKKGDFNKRRVYSTSPVDVALYWEKHGASRLHIVDLDGAKSGWPTHLKTIREIALRVNIPLQVG
GGIRSLKVIKKYLDSGVDRIILGTVALKNPELVKRALDNFGSNRIVVGVDARGGKVATEGWLKTSQVTVEDIISEMEEVG
VKTFIYTDINRDGMLKGPDIEGIKRVLKSTKARIIASGGISSRQDLINLKAIGIKAAIVGKALYEGNLPLEVLNQYP

Sequences:

>Translated_237_residues
MEVIPAVDIKDGSCVRLKKGDFNKRRVYSTSPVDVALYWEKHGASRLHIVDLDGAKSGWPTHLKTIREIALRVNIPLQVG
GGIRSLKVIKKYLDSGVDRIILGTVALKNPELVKRALDNFGSNRIVVGVDARGGKVATEGWLKTSQVTVEDIISEMEEVG
VKTFIYTDINRDGMLKGPDIEGIKRVLKSTKARIIASGGISSRQDLINLKAIGIKAAIVGKALYEGNLPLEVLNQYP
>Mature_237_residues
MEVIPAVDIKDGSCVRLKKGDFNKRRVYSTSPVDVALYWEKHGASRLHIVDLDGAKSGWPTHLKTIREIALRVNIPLQVG
GGIRSLKVIKKYLDSGVDRIILGTVALKNPELVKRALDNFGSNRIVVGVDARGGKVATEGWLKTSQVTVEDIISEMEEVG
VKTFIYTDINRDGMLKGPDIEGIKRVLKSTKARIIASGGISSRQDLINLKAIGIKAAIVGKALYEGNLPLEVLNQYP

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=235, Percent_Identity=35.3191489361702, Blast_Score=154, Evalue=4e-39,
Organism=Escherichia coli, GI1788336, Length=216, Percent_Identity=29.1666666666667, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_HALOH (B8D115)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002507979.1
- ProteinModelPortal:   B8D115
- SMR:   B8D115
- GeneID:   7312543
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_02230
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 25911; Mature: 25911

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVIPAVDIKDGSCVRLKKGDFNKRRVYSTSPVDVALYWEKHGASRLHIVDLDGAKSGWP
CCCCCCEECCCCCEEEEECCCCCCCEEEECCCCEEEEEEECCCCCEEEEEECCCCCCCCC
THLKTIREIALRVNIPLQVGGGIRSLKVIKKYLDSGVDRIILGTVALKNPELVKRALDNF
HHHHHHHHHHHEECCCEEECCCHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHC
GSNRIVVGVDARGGKVATEGWLKTSQVTVEDIISEMEEVGVKTFIYTDINRDGMLKGPDI
CCCEEEEEEECCCCEEECCCCEECCCEEHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCH
EGIKRVLKSTKARIIASGGISSRQDLINLKAIGIKAAIVGKALYEGNLPLEVLNQYP
HHHHHHHHHHHCEEEECCCCCCHHHHEEEEECCCHHHHHHHHHHCCCCCHHHHCCCC
>Mature Secondary Structure
MEVIPAVDIKDGSCVRLKKGDFNKRRVYSTSPVDVALYWEKHGASRLHIVDLDGAKSGWP
CCCCCCEECCCCCEEEEECCCCCCCEEEECCCCEEEEEEECCCCCEEEEEECCCCCCCCC
THLKTIREIALRVNIPLQVGGGIRSLKVIKKYLDSGVDRIILGTVALKNPELVKRALDNF
HHHHHHHHHHHEECCCEEECCCHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHC
GSNRIVVGVDARGGKVATEGWLKTSQVTVEDIISEMEEVGVKTFIYTDINRDGMLKGPDI
CCCEEEEEEECCCCEEECCCCEECCCEEHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCH
EGIKRVLKSTKARIIASGGISSRQDLINLKAIGIKAAIVGKALYEGNLPLEVLNQYP
HHHHHHHHHHHCEEEECCCCCCHHHHEEEEECCCHHHHHHHHHHCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA