Click here to switch to the map view.

The map label for this gene is hisH [H]

Identifier: 220931070

GI number: 220931070

Start: 229656

End: 230279

Strand: Direct

Name: hisH [H]

Synonym: Hore_02220

Alternate gene names: 220931070

Gene position: 229656-230279 (Clockwise)

Preceding gene: 220931069

Following gene: 220931071

Centisome position: 8.91

GC content: 41.51

Gene sequence:

>624_bases
GTGATTGTTGTTATTGATTATGGTATTGGAAATCTGGGGAGTGTTGTTAAAGCCTTTAAATATTTAGGTGTTCCTGTGAA
ATTAACAGCCAGCCCTGATGAAATCAGGGAAGCAGATGGAATAGTCTTACCCGGAGTCGGGGCCTTTGGACATGGGGTTG
AGAATTTAGAGAAATATAACCTGAAAAGGGTTATCAGGGAACTGATAGAAGAAGGAAAACCGTTTTTAGGTATATGCCTG
GGTATGCAGCTACTATTTTCCGGTAGTGAAGAGGCCCCGGGAGTTAGAGGACTGGGTATAATTAAAGGGATTGTCCAGAA
GTTTGACCCCTCAAACGTGGGCAAAATACCCCATATTGGCTGGAATAAAGTTAATATTATTAAAGAAGACCCTTTATTTT
ATAATCTCAATACCTCTCCTTATCTTTACTTTGTTCATAGCTTTTTTGCCCGGACATCGGAAGGGGATAATATCATCGGG
GAAACCTGTTATGGGAAACAGAGATTTGTCTCCGTTGTCAGGTCAAACAATGCCTGGGAAATACAGGGTCACCCAGAAAA
GAGTAGCCGGACGGGTTTGAAGATTCTTCAAAATTTCAGTGAGGTGGTTAACAGGTGGAAGTAA

Upstream 100 bases:

>100_bases
AAGGTTTTGGTCGAGCCCTGGATGTAGCATTGACCGGAGAAGAAAGGTTAAATAATACTCCCCTGTCCTCCAAAGGTTCT
CTGGGGGAGGGTGGTTAAAA

Downstream 100 bases:

>100_bases
TACCGGCAGTTGATATAAAAGATGGTAGTTGTGTCCGTCTTAAAAAAGGTGACTTTAATAAAAGGCGGGTCTACAGTACC
AGTCCAGTAGATGTGGCTCT

Product: imidazole glycerol phosphate synthase, glutamine amidotransferase subunit

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MIVVIDYGIGNLGSVVKAFKYLGVPVKLTASPDEIREADGIVLPGVGAFGHGVENLEKYNLKRVIRELIEEGKPFLGICL
GMQLLFSGSEEAPGVRGLGIIKGIVQKFDPSNVGKIPHIGWNKVNIIKEDPLFYNLNTSPYLYFVHSFFARTSEGDNIIG
ETCYGKQRFVSVVRSNNAWEIQGHPEKSSRTGLKILQNFSEVVNRWK

Sequences:

>Translated_207_residues
MIVVIDYGIGNLGSVVKAFKYLGVPVKLTASPDEIREADGIVLPGVGAFGHGVENLEKYNLKRVIRELIEEGKPFLGICL
GMQLLFSGSEEAPGVRGLGIIKGIVQKFDPSNVGKIPHIGWNKVNIIKEDPLFYNLNTSPYLYFVHSFFARTSEGDNIIG
ETCYGKQRFVSVVRSNNAWEIQGHPEKSSRTGLKILQNFSEVVNRWK
>Mature_207_residues
MIVVIDYGIGNLGSVVKAFKYLGVPVKLTASPDEIREADGIVLPGVGAFGHGVENLEKYNLKRVIRELIEEGKPFLGICL
GMQLLFSGSEEAPGVRGLGIIKGIVQKFDPSNVGKIPHIGWNKVNIIKEDPLFYNLNTSPYLYFVHSFFARTSEGDNIIG
ETCYGKQRFVSVVRSNNAWEIQGHPEKSSRTGLKILQNFSEVVNRWK

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=202, Percent_Identity=37.1287128712871, Blast_Score=119, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6319725, Length=209, Percent_Identity=40.6698564593301, Blast_Score=145, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22967; Mature: 22967

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVVIDYGIGNLGSVVKAFKYLGVPVKLTASPDEIREADGIVLPGVGAFGHGVENLEKYN
CEEEEECCCCCHHHHHHHHHHHCCCEEEECCHHHHHHCCCEEECCCCHHHCCHHHHHHHH
LKRVIRELIEEGKPFLGICLGMQLLFSGSEEAPGVRGLGIIKGIVQKFDPSNVGKIPHIG
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
WNKVNIIKEDPLFYNLNTSPYLYFVHSFFARTSEGDNIIGETCYGKQRFVSVVRSNNAWE
CCEEEEEECCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCEEE
IQGHPEKSSRTGLKILQNFSEVVNRWK
EECCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIVVIDYGIGNLGSVVKAFKYLGVPVKLTASPDEIREADGIVLPGVGAFGHGVENLEKYN
CEEEEECCCCCHHHHHHHHHHHCCCEEEECCHHHHHHCCCEEECCCCHHHCCHHHHHHHH
LKRVIRELIEEGKPFLGICLGMQLLFSGSEEAPGVRGLGIIKGIVQKFDPSNVGKIPHIG
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
WNKVNIIKEDPLFYNLNTSPYLYFVHSFFARTSEGDNIIGETCYGKQRFVSVVRSNNAWE
CCEEEEEECCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCEEE
IQGHPEKSSRTGLKILQNFSEVVNRWK
EECCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA