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The map label for this gene is hydN [C]

Identifier: 220931057

GI number: 220931057

Start: 214625

End: 216100

Strand: Direct

Name: hydN [C]

Synonym: Hore_02090

Alternate gene names: 220931057

Gene position: 214625-216100 (Clockwise)

Preceding gene: 220931056

Following gene: 220931058

Centisome position: 8.32

GC content: 43.36

Gene sequence:

>1476_bases
ATGGCAGGAGAAGTATCTGAAGTTACCAGGATCAGGAGAAAGGTTTTGACCGAGATAGCCCGTCTTACTTTTGAAGATCG
TCTGGTGGAAGAGGTAGATTATATTCCCCGGAGGTTGACTGAAAATGGTATTTCCAATTATCGCTGTTGTGTCTATAAGG
AAAGAGCAATTTTAAAAGAGAGGGTTAAACTGGCCCTGGGGGTTGCCCCTGATGAAGTGGATGATGAAGAGAAGAGTCTT
TCTGAAATAGCGAGTAATGTATTACAGGGCCAACTGGGGAACAGTGATAGAAATATTGCTATTATTGAAGAAGCCTGTGA
CCAGTGTTCAATCGATAAAATAGTGGTAACCAATGCCTGTCGTAATTGTGTTGCCCACCACTGTGTAAATTCCTGTCCCC
GGGGCGCCATTACCATTGTTAATAATCAGGCCTATGTTATCAGGGAAAAGTGTGTGGAGTGCGGGCTCTGTGTTAAAGCC
TGCCCCTATGGCGCCATTCTTGAAGTTGAAAGACCCTGTACCAGTGCCTGTTCCCTTGATGCAGTTGTTCCCGGGGAGAA
GAGTACAGCAGAAATAGATGATAATAATTGTATTGAATGTGGCTCCTGTATAGAGGCCTGTCCCTTTGGAGCAATTTCCT
ATAAATCTGAAATTGTCAGGGTTGTTCAAATGTTAAAAAGTGGAGACATTAATACTGTAGCCCTGGTTGCTCCTTCATAT
ATTGGACAGTTTGGACCAAGGGTTGACTGGGAAAAACTATGTACTGGTCTTAAAAGGCTGGGATTTCACGATGTAATACC
GGTGGCCCTCGGTGCTGATAAGGTAATTGAAGAGGAAAGCAGGGAATTTCAGGCGATGACTGATAAACCAATGTTTAATT
CCTGTTGTCCTTCCTTTGTTAACTTAATAGAATTAAAATTTCCTGAATATTTATCACAGGTATCTACTACAGAATCACCG
ATGCTTAAAGCTGCTTATCTGGCTAAAGAAGATGGTGAACTGGTTCAGTGTGTCTTTATCGGCCCCTGTCTTGCTAAAAA
GAGTGAGGCCAGGAATAAGGGGCAGGGTTTGATAGATGCTGTTTTGACCTTTGAAGAAATTGCAGCCATGCTGGTGGCTA
AAGGTATTAACCTGGCAAAAATAACCGAAACGGGGGATCTATCTGAAGAACACCGGTCCCCTTCCATACCGGCTCAGGCC
TTCTGTGAAGCCGGGGGAGTTGGAAGTGCTATCAGTGGAAAACTTAATAATAATGATAATGGTATAAGGTTTCACCAGGT
TGATGGTGCTTCAGAATGTCTTAAAGTGTTAAATCAGATTAAAGCAGGTAAGATTAAAGCAGATTTTGTTGAAGGTATGG
GTTGCCAGGGGGGCTGTATTGGTGGCCCCGGAACGCTGGTAAACCGGCGAGTGGCTTCCGGATTATTAAAAAAGCTTATA
AAATCCAGGGGTGGTGAAAAAGTTGGCACAAAGTAA

Upstream 100 bases:

>100_bases
GGTAATGGTTAACGACACTGTAATTTATAATGCAACACCGGAGCGGGTTAAGGAAGCAATTACTGAAATAATTATGGCAT
AAAGGGGTAGGTGATAAGAT

Downstream 100 bases:

>100_bases
AGGGAGGGTTAAATCACAGGGGATACCGGTAGGAGTCATAAATCGTTTGCCCCATTATTACCGTTATTTGAGTTCACTTA
TGGCAAGGGATGTGGAAAGA

Product: hydrogenase large subunit domain-containing protein

Products: NA

Alternate protein names: CpI; Fe-only hydrogenase; [Fe] hydrogenase [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MAGEVSEVTRIRRKVLTEIARLTFEDRLVEEVDYIPRRLTENGISNYRCCVYKERAILKERVKLALGVAPDEVDDEEKSL
SEIASNVLQGQLGNSDRNIAIIEEACDQCSIDKIVVTNACRNCVAHHCVNSCPRGAITIVNNQAYVIREKCVECGLCVKA
CPYGAILEVERPCTSACSLDAVVPGEKSTAEIDDNNCIECGSCIEACPFGAISYKSEIVRVVQMLKSGDINTVALVAPSY
IGQFGPRVDWEKLCTGLKRLGFHDVIPVALGADKVIEEESREFQAMTDKPMFNSCCPSFVNLIELKFPEYLSQVSTTESP
MLKAAYLAKEDGELVQCVFIGPCLAKKSEARNKGQGLIDAVLTFEEIAAMLVAKGINLAKITETGDLSEEHRSPSIPAQA
FCEAGGVGSAISGKLNNNDNGIRFHQVDGASECLKVLNQIKAGKIKADFVEGMGCQGGCIGGPGTLVNRRVASGLLKKLI
KSRGGEKVGTK

Sequences:

>Translated_491_residues
MAGEVSEVTRIRRKVLTEIARLTFEDRLVEEVDYIPRRLTENGISNYRCCVYKERAILKERVKLALGVAPDEVDDEEKSL
SEIASNVLQGQLGNSDRNIAIIEEACDQCSIDKIVVTNACRNCVAHHCVNSCPRGAITIVNNQAYVIREKCVECGLCVKA
CPYGAILEVERPCTSACSLDAVVPGEKSTAEIDDNNCIECGSCIEACPFGAISYKSEIVRVVQMLKSGDINTVALVAPSY
IGQFGPRVDWEKLCTGLKRLGFHDVIPVALGADKVIEEESREFQAMTDKPMFNSCCPSFVNLIELKFPEYLSQVSTTESP
MLKAAYLAKEDGELVQCVFIGPCLAKKSEARNKGQGLIDAVLTFEEIAAMLVAKGINLAKITETGDLSEEHRSPSIPAQA
FCEAGGVGSAISGKLNNNDNGIRFHQVDGASECLKVLNQIKAGKIKADFVEGMGCQGGCIGGPGTLVNRRVASGLLKKLI
KSRGGEKVGTK
>Mature_490_residues
AGEVSEVTRIRRKVLTEIARLTFEDRLVEEVDYIPRRLTENGISNYRCCVYKERAILKERVKLALGVAPDEVDDEEKSLS
EIASNVLQGQLGNSDRNIAIIEEACDQCSIDKIVVTNACRNCVAHHCVNSCPRGAITIVNNQAYVIREKCVECGLCVKAC
PYGAILEVERPCTSACSLDAVVPGEKSTAEIDDNNCIECGSCIEACPFGAISYKSEIVRVVQMLKSGDINTVALVAPSYI
GQFGPRVDWEKLCTGLKRLGFHDVIPVALGADKVIEEESREFQAMTDKPMFNSCCPSFVNLIELKFPEYLSQVSTTESPM
LKAAYLAKEDGELVQCVFIGPCLAKKSEARNKGQGLIDAVLTFEEIAAMLVAKGINLAKITETGDLSEEHRSPSIPAQAF
CEAGGVGSAISGKLNNNDNGIRFHQVDGASECLKVLNQIKAGKIKADFVEGMGCQGGCIGGPGTLVNRRVASGLLKKLIK
SRGGEKVGTK

Specific function: Electron Transport From Formate To Hydrogen. [C]

COG id: COG4624

COG function: function code R; Iron only hydrogenase large subunit, C-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012675
- InterPro:   IPR009016
- InterPro:   IPR004108
- InterPro:   IPR003149
- InterPro:   IPR013352
- InterPro:   IPR001041 [H]

Pfam domain/function: PF02906 Fe_hyd_lg_C; PF02256 Fe_hyd_SSU [H]

EC number: =1.12.7.2 [H]

Molecular weight: Translated: 53083; Mature: 52951

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
5.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGEVSEVTRIRRKVLTEIARLTFEDRLVEEVDYIPRRLTENGISNYRCCVYKERAILKE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHH
RVKLALGVAPDEVDDEEKSLSEIASNVLQGQLGNSDRNIAIIEEACDQCSIDKIVVTNAC
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHCCCCCCHHHHHHHH
RNCVAHHCVNSCPRGAITIVNNQAYVIREKCVECGLCVKACPYGAILEVERPCTSACSLD
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCHHHHCCCCCEEEECCCCCCCCCCC
AVVPGEKSTAEIDDNNCIECGSCIEACPFGAISYKSEIVRVVQMLKSGDINTVALVAPSY
CCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCHH
IGQFGPRVDWEKLCTGLKRLGFHDVIPVALGADKVIEEESREFQAMTDKPMFNSCCPSFV
HHHCCCCCCHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
NLIELKFPEYLSQVSTTESPMLKAAYLAKEDGELVQCVFIGPCLAKKSEARNKGQGLIDA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHCCCHHHHH
VLTFEEIAAMLVAKGINLAKITETGDLSEEHRSPSIPAQAFCEAGGVGSAISGKLNNNDN
HHHHHHHHHHHHHCCCCEEEECCCCCCCHHHCCCCCCHHHHHHCCCCCCHHCCCCCCCCC
GIRFHQVDGASECLKVLNQIKAGKIKADFVEGMGCQGGCIGGPGTLVNRRVASGLLKKLI
CEEEEECCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KSRGGEKVGTK
HHCCCCCCCCC
>Mature Secondary Structure 
AGEVSEVTRIRRKVLTEIARLTFEDRLVEEVDYIPRRLTENGISNYRCCVYKERAILKE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHH
RVKLALGVAPDEVDDEEKSLSEIASNVLQGQLGNSDRNIAIIEEACDQCSIDKIVVTNAC
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHCCCCCCHHHHHHHH
RNCVAHHCVNSCPRGAITIVNNQAYVIREKCVECGLCVKACPYGAILEVERPCTSACSLD
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCHHHHCCCCCEEEECCCCCCCCCCC
AVVPGEKSTAEIDDNNCIECGSCIEACPFGAISYKSEIVRVVQMLKSGDINTVALVAPSY
CCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCHH
IGQFGPRVDWEKLCTGLKRLGFHDVIPVALGADKVIEEESREFQAMTDKPMFNSCCPSFV
HHHCCCCCCHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
NLIELKFPEYLSQVSTTESPMLKAAYLAKEDGELVQCVFIGPCLAKKSEARNKGQGLIDA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHCCCHHHHH
VLTFEEIAAMLVAKGINLAKITETGDLSEEHRSPSIPAQAFCEAGGVGSAISGKLNNNDN
HHHHHHHHHHHHHCCCCEEEECCCCCCCHHHCCCCCCHHHHHHCCCCCCHHCCCCCCCCC
GIRFHQVDGASECLKVLNQIKAGKIKADFVEGMGCQGGCIGGPGTLVNRRVASGLLKKLI
CEEEEECCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KSRGGEKVGTK
HHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1911757; 9836629; 10529166 [H]