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The map label for this gene is nuoF2 [H]

Identifier: 220931029

GI number: 220931029

Start: 185624

End: 187498

Strand: Direct

Name: nuoF2 [H]

Synonym: Hore_01800

Alternate gene names: 220931029

Gene position: 185624-187498 (Clockwise)

Preceding gene: 220931028

Following gene: 220931030

Centisome position: 7.2

GC content: 43.47

Gene sequence:

>1875_bases
ATGATCAAGAGTAAGACTGATTTAGAAGAGGCAATTAAAAAAGCTAAAGATAAGCTGCAGGAAGAAGGGTTAAGGATTCT
TGTCTGTGCCGGAACCGGATGTGTGGCCGGAGGTTCCCTTGAAATCTTTGATGAACTCAAAAAGAAAGTGGAGAATTTAA
GTTTACCGGTTAAAGTAGGATTGCTTGAAGAAAAGGAGAGCAATGTTACAGTAAAAACCAGTGGATGTCATGGATTTTGT
GAAAAAGGTCCACTGGTAAAAATTGAACCCCTTGGTATTTTATTTACTAAAGTAAGCCAGGATGATGTTGATGAGATCAT
TAATGATACTATTAAAGCCGGGAAATTAGTAGATAAATTAATTTATAAAAGCCCTGATGGTGAGGCTTATTCTAATGAAA
AAGAAATACCATTTTATGCAAACCAGAACCGGATTGCTTTAAGTAACTGTGGTAATATAGATCCGGAAGACCTTGATGAT
TATCTGGCCCATGGTGGGTATAAGGCTCTGAGTAAGGCCTTACTGGAAATGAGTCCAGAAGAAGTATGTAAAGAAGTTAC
CGAGTCGGGCCTCCGTGGCCGTGGTGGAGGTGGCTTTCCTACCGGCAAGAAGTGGGAGTTTGCTTATCGGGAAAAGGCTG
ATCAGAAGTATGTAATTGTAAATGGTGACGAAGGTGACCCCGGAGCCTTTATGGACAGAAGTATTATGGAAGGTGATCCT
CACCGGGTTATTGAAGGTATTACTATTGCTGGTTATGCCACTGGAGCCACCAAAGGTTATATTTATGTCAGGGCTGAATA
CCCTCTGGCTGTTAAACGTTTGAGAAAAGCCATTAATGATGCTTATGATCAAGGTTTACTCGGTGAAGATATCCTGGGAA
GTGGCTTCGACTTTGATCTTATGATAAAAGAAGGGGCCGGGGCTTTTGTCTGTGGTGAAGAGACTGCCCTGATGGCTTCT
ATTGAAGGTAAGAGGGGTATGCCCAATCCGAAACCACCGTTCCCTGCCCAGTCAGGGTTATGGGGTAAACCTACAACCAT
CAATAATGTGGAAACCCTGGCCAATGTTCCCCAGATTATTCTTAAAGGTGCTGACTGGTTTACCAGCCTGGGTACTGAAA
ATAGTCCGGGAACCAAAACCTTTGCTATTACCGGTGATGTTGCCAATACCGGCTTGATTGAAGTACCCATGGGTATGACC
CTGAGGGAACTGGTCTATGATATCGGTGGAGGTATTAAGAATAATAATAAGTTTAAAGCAGTTCAGATCGGTGGTCCTTC
CGGAGGGTGTCTGACTGAAGAGCACCTGGATATGCCCATGGATTTTGACTCCCTGCAGGAAGTAGGCGCCATGATTGGAT
CCGGTGGTCTTGTTGTCATGGATGAAAATACCTGTATGGTAGAAGTGGCCCGTTTCTTTATGGAGTTTACCCAGAACGAA
TCCTGTGGAAAATGTGTTCTCTGTCGTGAAGGGACTAAACAGATGTTAAAGATATTGGAGAGAATTGTTGAAGGTAAAGG
TACCATGGAAGACCTGGATTTACTGGAAGAACTGGCTTTAGCTGTTAAAGACGGGTCCCTCTGCGGGCTTGGTAAAACTG
CTCCGAACCCGGTTTTAACAACCCTGAAATACTTCCGGGATGAATATGAAGCCCATGTTAAGGACAAGAAATGTCCGGCC
GGTGTCTGTGAGGCCCTGAAATCCTATAAGATTAATCCTGACTTATGTAAGGGTTGTAGTCTATGTGCCCGTAAGTGTCC
TGTTGATGCAATCAGTGGTAAGGTTAAAGAGCCCTTTGTAATTGATCAGGATAAATGTATTAAGTGTGGTGCCTGTTATG
AGGCCTGTAAATTTAACGCTGTGGAGGTGGGATAA

Upstream 100 bases:

>100_bases
GGTCTGGCCCCTGTAGTGACAATAAATGGTAAGGTTTATGGAAAAATGACTCCTGAAGCCATTGAGGTAATTATAGATGA
ACTTATGGGGAGGGATACAG

Downstream 100 bases:

>100_bases
ATGTCTGATACTTTAGTAATAAACGGAAAAACTGTTGCGATAAATGGTGAGAAGAATCTGCTTGAGCTGATAAGAAAACA
GGGGATTGAGCTACCAACCT

Product: NADH dehydrogenase I subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 624; Mature: 624

Protein sequence:

>624_residues
MIKSKTDLEEAIKKAKDKLQEEGLRILVCAGTGCVAGGSLEIFDELKKKVENLSLPVKVGLLEEKESNVTVKTSGCHGFC
EKGPLVKIEPLGILFTKVSQDDVDEIINDTIKAGKLVDKLIYKSPDGEAYSNEKEIPFYANQNRIALSNCGNIDPEDLDD
YLAHGGYKALSKALLEMSPEEVCKEVTESGLRGRGGGGFPTGKKWEFAYREKADQKYVIVNGDEGDPGAFMDRSIMEGDP
HRVIEGITIAGYATGATKGYIYVRAEYPLAVKRLRKAINDAYDQGLLGEDILGSGFDFDLMIKEGAGAFVCGEETALMAS
IEGKRGMPNPKPPFPAQSGLWGKPTTINNVETLANVPQIILKGADWFTSLGTENSPGTKTFAITGDVANTGLIEVPMGMT
LRELVYDIGGGIKNNNKFKAVQIGGPSGGCLTEEHLDMPMDFDSLQEVGAMIGSGGLVVMDENTCMVEVARFFMEFTQNE
SCGKCVLCREGTKQMLKILERIVEGKGTMEDLDLLEELALAVKDGSLCGLGKTAPNPVLTTLKYFRDEYEAHVKDKKCPA
GVCEALKSYKINPDLCKGCSLCARKCPVDAISGKVKEPFVIDQDKCIKCGACYEACKFNAVEVG

Sequences:

>Translated_624_residues
MIKSKTDLEEAIKKAKDKLQEEGLRILVCAGTGCVAGGSLEIFDELKKKVENLSLPVKVGLLEEKESNVTVKTSGCHGFC
EKGPLVKIEPLGILFTKVSQDDVDEIINDTIKAGKLVDKLIYKSPDGEAYSNEKEIPFYANQNRIALSNCGNIDPEDLDD
YLAHGGYKALSKALLEMSPEEVCKEVTESGLRGRGGGGFPTGKKWEFAYREKADQKYVIVNGDEGDPGAFMDRSIMEGDP
HRVIEGITIAGYATGATKGYIYVRAEYPLAVKRLRKAINDAYDQGLLGEDILGSGFDFDLMIKEGAGAFVCGEETALMAS
IEGKRGMPNPKPPFPAQSGLWGKPTTINNVETLANVPQIILKGADWFTSLGTENSPGTKTFAITGDVANTGLIEVPMGMT
LRELVYDIGGGIKNNNKFKAVQIGGPSGGCLTEEHLDMPMDFDSLQEVGAMIGSGGLVVMDENTCMVEVARFFMEFTQNE
SCGKCVLCREGTKQMLKILERIVEGKGTMEDLDLLEELALAVKDGSLCGLGKTAPNPVLTTLKYFRDEYEAHVKDKKCPA
GVCEALKSYKINPDLCKGCSLCARKCPVDAISGKVKEPFVIDQDKCIKCGACYEACKFNAVEVG
>Mature_624_residues
MIKSKTDLEEAIKKAKDKLQEEGLRILVCAGTGCVAGGSLEIFDELKKKVENLSLPVKVGLLEEKESNVTVKTSGCHGFC
EKGPLVKIEPLGILFTKVSQDDVDEIINDTIKAGKLVDKLIYKSPDGEAYSNEKEIPFYANQNRIALSNCGNIDPEDLDD
YLAHGGYKALSKALLEMSPEEVCKEVTESGLRGRGGGGFPTGKKWEFAYREKADQKYVIVNGDEGDPGAFMDRSIMEGDP
HRVIEGITIAGYATGATKGYIYVRAEYPLAVKRLRKAINDAYDQGLLGEDILGSGFDFDLMIKEGAGAFVCGEETALMAS
IEGKRGMPNPKPPFPAQSGLWGKPTTINNVETLANVPQIILKGADWFTSLGTENSPGTKTFAITGDVANTGLIEVPMGMT
LRELVYDIGGGIKNNNKFKAVQIGGPSGGCLTEEHLDMPMDFDSLQEVGAMIGSGGLVVMDENTCMVEVARFFMEFTQNE
SCGKCVLCREGTKQMLKILERIVEGKGTMEDLDLLEELALAVKDGSLCGLGKTAPNPVLTTLKYFRDEYEAHVKDKKCPA
GVCEALKSYKINPDLCKGCSLCARKCPVDAISGKVKEPFVIDQDKCIKCGACYEACKFNAVEVG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=417, Percent_Identity=40.0479616306954, Blast_Score=311, Evalue=1e-84,
Organism=Homo sapiens, GI260656005, Length=417, Percent_Identity=40.0479616306954, Blast_Score=311, Evalue=1e-84,
Organism=Escherichia coli, GI1788620, Length=404, Percent_Identity=43.8118811881188, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17531727, Length=401, Percent_Identity=41.3965087281795, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI45550452, Length=389, Percent_Identity=41.1311053984576, Blast_Score=314, Evalue=9e-86,
Organism=Drosophila melanogaster, GI19920794, Length=418, Percent_Identity=39.2344497607655, Blast_Score=305, Evalue=6e-83,
Organism=Drosophila melanogaster, GI24653819, Length=401, Percent_Identity=33.6658354114713, Blast_Score=240, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24653817, Length=353, Percent_Identity=32.0113314447592, Blast_Score=206, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 67540; Mature: 67540

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKSKTDLEEAIKKAKDKLQEEGLRILVCAGTGCVAGGSLEIFDELKKKVENLSLPVKVG
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEE
LLEEKESNVTVKTSGCHGFCEKGPLVKIEPLGILFTKVSQDDVDEIINDTIKAGKLVDKL
EEECCCCCEEEEECCCCCCCCCCCEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHHHHH
IYKSPDGEAYSNEKEIPFYANQNRIALSNCGNIDPEDLDDYLAHGGYKALSKALLEMSPE
HHCCCCCCCCCCCCCCCEEECCCEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCHH
EVCKEVTESGLRGRGGGGFPTGKKWEFAYREKADQKYVIVNGDEGDPGAFMDRSIMEGDP
HHHHHHHHCCCCCCCCCCCCCCCCEEEHHHHCCCCEEEEEECCCCCCCHHHCCHHHCCCH
HRVIEGITIAGYATGATKGYIYVRAEYPLAVKRLRKAINDAYDQGLLGEDILGSGFDFDL
HHHHCCEEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCEEE
MIKEGAGAFVCGEETALMASIEGKRGMPNPKPPFPAQSGLWGKPTTINNVETLANVPQII
EEECCCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LKGADWFTSLGTENSPGTKTFAITGDVANTGLIEVPMGMTLRELVYDIGGGIKNNNKFKA
HCCCHHHHHCCCCCCCCCEEEEEECCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCEEE
VQIGGPSGGCLTEEHLDMPMDFDSLQEVGAMIGSGGLVVMDENTCMVEVARFFMEFTQNE
EEECCCCCCCCCHHHCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCC
SCGKCVLCREGTKQMLKILERIVEGKGTMEDLDLLEELALAVKDGSLCGLGKTAPNPVLT
CCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHH
TLKYFRDEYEAHVKDKKCPAGVCEALKSYKINPDLCKGCSLCARKCPVDAISGKVKEPFV
HHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCEE
IDQDKCIKCGACYEACKFNAVEVG
ECCCCCEECHHHHHHHCCCEEECC
>Mature Secondary Structure
MIKSKTDLEEAIKKAKDKLQEEGLRILVCAGTGCVAGGSLEIFDELKKKVENLSLPVKVG
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEE
LLEEKESNVTVKTSGCHGFCEKGPLVKIEPLGILFTKVSQDDVDEIINDTIKAGKLVDKL
EEECCCCCEEEEECCCCCCCCCCCEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHHHHH
IYKSPDGEAYSNEKEIPFYANQNRIALSNCGNIDPEDLDDYLAHGGYKALSKALLEMSPE
HHCCCCCCCCCCCCCCCEEECCCEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCHH
EVCKEVTESGLRGRGGGGFPTGKKWEFAYREKADQKYVIVNGDEGDPGAFMDRSIMEGDP
HHHHHHHHCCCCCCCCCCCCCCCCEEEHHHHCCCCEEEEEECCCCCCCHHHCCHHHCCCH
HRVIEGITIAGYATGATKGYIYVRAEYPLAVKRLRKAINDAYDQGLLGEDILGSGFDFDL
HHHHCCEEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCEEE
MIKEGAGAFVCGEETALMASIEGKRGMPNPKPPFPAQSGLWGKPTTINNVETLANVPQII
EEECCCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LKGADWFTSLGTENSPGTKTFAITGDVANTGLIEVPMGMTLRELVYDIGGGIKNNNKFKA
HCCCHHHHHCCCCCCCCCEEEEEECCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCEEE
VQIGGPSGGCLTEEHLDMPMDFDSLQEVGAMIGSGGLVVMDENTCMVEVARFFMEFTQNE
EEECCCCCCCCCHHHCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCC
SCGKCVLCREGTKQMLKILERIVEGKGTMEDLDLLEELALAVKDGSLCGLGKTAPNPVLT
CCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHH
TLKYFRDEYEAHVKDKKCPAGVCEALKSYKINPDLCKGCSLCARKCPVDAISGKVKEPFV
HHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCEE
IDQDKCIKCGACYEACKFNAVEVG
ECCCCCEECHHHHHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]