Click here to switch to the map view.

The map label for this gene is tufA [H]

Identifier: 220930949

GI number: 220930949

Start: 119180

End: 120373

Strand: Direct

Name: tufA [H]

Synonym: Hore_01010

Alternate gene names: 220930949

Gene position: 119180-120373 (Clockwise)

Preceding gene: 220930948

Following gene: 220930950

Centisome position: 4.62

GC content: 44.64

Gene sequence:

>1194_bases
ATGGCAAAAGAAAAATTTGAAAGGACAAAACCGCATGTTAATATTGGAACCATAGGTCACGTAGACCACGGTAAGACTAC
TTTAACAGCAGCGATAACAGCTGTATTATCAAATCTGGGTAGTGCTGAAGTAATGCCCTTTGATTCTATCGATAATGCAC
CTGAAGAAAAAGAAAGAGGAATAACCATTGCTACCTCTCACGTTGAATATGAGACTGCTAATAGGCACTATGCCCACGTT
GACTGTCCAGGTCATGCTGACTATGTAAAGAATATGATTACCGGGGCCGCCCAGATGGACGGAGCTATTCTGGTAGTATC
AGCAGCAGACGGACCGATGCCCCAGACCAGAGAACATATTCTTCTGGCCCGTCAGGTAGGTGTACCGAGCATAGTTGTCT
TTTTAAACAAGGCCGATATGGTAGATGACGAAGAGTTAATTGAGCTGGTAGAGATGGAAGTAAGGGAATTATTAAATGAA
TATGAATTCCCTGGAGATGACGTACCGGTAATAGTTGGATCAGCATTACAGGCTCTTGAAAATCCTGATCCTGAAGGAGA
ATGGGGTAAGAAGATCATTGAACTTATGGAAGCAGTAGATGAATACATTCCCGAACCAGAAAGGGATAAAGACAAACCAT
TCTTGATGCCAGTAGAAGACGTATTCAGTATTACCGGACGTGGAACAGTAGCTACCGGTAGGGTAGAGAGAGGAACCTTA
CATCCCGGTGACGAGGTAGAAATTATCGGGATTAAAGAGACCCAGGAGACCGTAGTAACCGGGGTAGAGATGTTCCGTAA
GTTACTGGATGAAGCGGTAGCAGGAGATAACATTGGAGCCCTGTTAAGGGGTATAGGAAGAGATGAGATAGAAAGGGGTC
AGGTATTAGCCAAGCCAGGGAGTATAACCCCACATACCCACTTCAAGGCTGAGGTATATGTTCTGACCAAAGAAGAAGGA
GGAAGGCATACTCCATTTTTTGAAGGATACAGGCCCCAGTTTTATTTCAGGACCACAGACGTAACCGGGACTATCAGCTT
ACCAGAGGGAGTAGAGATGGTAATGCCCGGTGACAATGTAGAGATGGAAGTAAAATTAATTACTCCCATTGCTATGGAAG
AAGGATTAAGGTTTGCTATCCGTGAAGGTGGCCGTACCGTTGGTGCCGGTGTTGTCAGCCAGATTATTGAGTAA

Upstream 100 bases:

>100_bases
CCATCCGGTGGTGCTTTTACATAATGACTGCTATAATTAAAAAGTATTTCTGGCAGCAGTCACTTGCATTACAAGACTAA
AACAAGGAGGATGAAAAAGG

Downstream 100 bases:

>100_bases
AAATATCTCCCTCTCTTAGTTGAGATAGTTTAACAGGAATGGTTTGGACAATAAAAGCATGTGCTCTTTGGTGGAGCACA
TGCTTAAAAATTGATGTTGA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu-A [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MAKEKFERTKPHVNIGTIGHVDHGKTTLTAAITAVLSNLGSAEVMPFDSIDNAPEEKERGITIATSHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPSIVVFLNKADMVDDEELIELVEMEVRELLNE
YEFPGDDVPVIVGSALQALENPDPEGEWGKKIIELMEAVDEYIPEPERDKDKPFLMPVEDVFSITGRGTVATGRVERGTL
HPGDEVEIIGIKETQETVVTGVEMFRKLLDEAVAGDNIGALLRGIGRDEIERGQVLAKPGSITPHTHFKAEVYVLTKEEG
GRHTPFFEGYRPQFYFRTTDVTGTISLPEGVEMVMPGDNVEMEVKLITPIAMEEGLRFAIREGGRTVGAGVVSQIIE

Sequences:

>Translated_397_residues
MAKEKFERTKPHVNIGTIGHVDHGKTTLTAAITAVLSNLGSAEVMPFDSIDNAPEEKERGITIATSHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPSIVVFLNKADMVDDEELIELVEMEVRELLNE
YEFPGDDVPVIVGSALQALENPDPEGEWGKKIIELMEAVDEYIPEPERDKDKPFLMPVEDVFSITGRGTVATGRVERGTL
HPGDEVEIIGIKETQETVVTGVEMFRKLLDEAVAGDNIGALLRGIGRDEIERGQVLAKPGSITPHTHFKAEVYVLTKEEG
GRHTPFFEGYRPQFYFRTTDVTGTISLPEGVEMVMPGDNVEMEVKLITPIAMEEGLRFAIREGGRTVGAGVVSQIIE
>Mature_396_residues
AKEKFERTKPHVNIGTIGHVDHGKTTLTAAITAVLSNLGSAEVMPFDSIDNAPEEKERGITIATSHVEYETANRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPSIVVFLNKADMVDDEELIELVEMEVRELLNEY
EFPGDDVPVIVGSALQALENPDPEGEWGKKIIELMEAVDEYIPEPERDKDKPFLMPVEDVFSITGRGTVATGRVERGTLH
PGDEVEIIGIKETQETVVTGVEMFRKLLDEAVAGDNIGALLRGIGRDEIERGQVLAKPGSITPHTHFKAEVYVLTKEEGG
RHTPFFEGYRPQFYFRTTDVTGTISLPEGVEMVMPGDNVEMEVKLITPIAMEEGLRFAIREGGRTVGAGVVSQIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=57.9746835443038, Blast_Score=478, Evalue=1e-135,
Organism=Homo sapiens, GI223555963, Length=443, Percent_Identity=30.4740406320542, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI5729864, Length=443, Percent_Identity=30.4740406320542, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI4503475, Length=442, Percent_Identity=30.0904977375566, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI4503471, Length=442, Percent_Identity=29.6380090497738, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI54607086, Length=347, Percent_Identity=30.5475504322767, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI46094014, Length=373, Percent_Identity=28.1501340482574, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI194018522, Length=338, Percent_Identity=28.1065088757396, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI194018520, Length=338, Percent_Identity=28.1065088757396, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI194097354, Length=338, Percent_Identity=28.1065088757396, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI4503507, Length=278, Percent_Identity=28.0575539568345, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI157426893, Length=268, Percent_Identity=25, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=34.8484848484849, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI82546879, Length=435, Percent_Identity=23.448275862069, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI310132016, Length=109, Percent_Identity=35.7798165137615, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI310110807, Length=109, Percent_Identity=35.7798165137615, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI310123363, Length=109, Percent_Identity=35.7798165137615, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=76.7676767676768, Blast_Score=603, Evalue=1e-174,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=76.7676767676768, Blast_Score=603, Evalue=1e-174,
Organism=Escherichia coli, GI2367247, Length=380, Percent_Identity=30, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI48994988, Length=238, Percent_Identity=32.7731092436975, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1789108, Length=334, Percent_Identity=29.0419161676647, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788922, Length=331, Percent_Identity=25.6797583081571, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17556456, Length=405, Percent_Identity=52.3456790123457, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI25141371, Length=388, Percent_Identity=44.8453608247423, Blast_Score=342, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI17552884, Length=449, Percent_Identity=29.8440979955457, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=449, Percent_Identity=29.8440979955457, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI32566303, Length=427, Percent_Identity=29.03981264637, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI32566629, Length=452, Percent_Identity=27.212389380531, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI115532067, Length=433, Percent_Identity=27.7136258660508, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=430, Percent_Identity=27.6744186046512, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=301, Percent_Identity=29.2358803986711, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=37.012987012987, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17509919, Length=282, Percent_Identity=27.3049645390071, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=238, Percent_Identity=27.3109243697479, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=66.8341708542714, Blast_Score=540, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6325337, Length=440, Percent_Identity=29.7727272727273, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=440, Percent_Identity=29.7727272727273, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=455, Percent_Identity=24.6153846153846, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320377, Length=342, Percent_Identity=27.1929824561404, Blast_Score=89, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=271, Percent_Identity=27.6752767527675, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=259, Percent_Identity=29.3436293436293, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=176, Percent_Identity=30.1136363636364, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281363316, Length=395, Percent_Identity=56.9620253164557, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI17864358, Length=395, Percent_Identity=56.9620253164557, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI19921738, Length=388, Percent_Identity=50, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI45553807, Length=457, Percent_Identity=29.3216630196937, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI45553816, Length=457, Percent_Identity=29.3216630196937, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24651721, Length=457, Percent_Identity=29.3216630196937, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI17864154, Length=457, Percent_Identity=29.3216630196937, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24652838, Length=457, Percent_Identity=29.5404814004376, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI17137572, Length=457, Percent_Identity=29.5404814004376, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI19922690, Length=306, Percent_Identity=31.3725490196078, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI45550900, Length=451, Percent_Identity=28.159645232816, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI17137380, Length=347, Percent_Identity=29.3948126801153, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24647048, Length=431, Percent_Identity=25.9860788863109, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24647046, Length=431, Percent_Identity=25.9860788863109, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI78706572, Length=259, Percent_Identity=27.027027027027, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28572034, Length=233, Percent_Identity=35.6223175965665, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28574573, Length=146, Percent_Identity=33.5616438356164, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43521; Mature: 43390

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERTKPHVNIGTIGHVDHGKTTLTAAITAVLSNLGSAEVMPFDSIDNAPEEKERG
CCCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHCC
ITIATSHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPSIVVFLNKADMVDDEELIELVEMEVRELLNEYEFPGDDVPVIVGSALQALE
HHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHC
NPDPEGEWGKKIIELMEAVDEYIPEPERDKDKPFLMPVEDVFSITGRGTVATGRVERGTL
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHCCCCEEEECCCCCCCC
HPGDEVEIIGIKETQETVVTGVEMFRKLLDEAVAGDNIGALLRGIGRDEIERGQVLAKPG
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHCCCEEECCC
SITPHTHFKAEVYVLTKEEGGRHTPFFEGYRPQFYFRTTDVTGTISLPEGVEMVMPGDNV
CCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCC
EMEVKLITPIAMEEGLRFAIREGGRTVGAGVVSQIIE
EEEEEEECCHHHHHCCEEEHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AKEKFERTKPHVNIGTIGHVDHGKTTLTAAITAVLSNLGSAEVMPFDSIDNAPEEKERG
CCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHCC
ITIATSHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPSIVVFLNKADMVDDEELIELVEMEVRELLNEYEFPGDDVPVIVGSALQALE
HHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHC
NPDPEGEWGKKIIELMEAVDEYIPEPERDKDKPFLMPVEDVFSITGRGTVATGRVERGTL
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHCCCCEEEECCCCCCCC
HPGDEVEIIGIKETQETVVTGVEMFRKLLDEAVAGDNIGALLRGIGRDEIERGQVLAKPG
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHCCCEEECCC
SITPHTHFKAEVYVLTKEEGGRHTPFFEGYRPQFYFRTTDVTGTISLPEGVEMVMPGDNV
CCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCC
EMEVKLITPIAMEEGLRFAIREGGRTVGAGVVSQIIE
EEEEEEECCHHHHHCCEEEHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]