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The map label for this gene is sigH [H]

Identifier: 220930948

GI number: 220930948

Start: 118418

End: 119065

Strand: Direct

Name: sigH [H]

Synonym: Hore_01000

Alternate gene names: 220930948

Gene position: 118418-119065 (Clockwise)

Preceding gene: 220930947

Following gene: 220930949

Centisome position: 4.59

GC content: 37.65

Gene sequence:

>648_bases
TTGAAGGGAAATGCACAGGAAAAATATGATTATGAAGATTATAATCAATTGGGGGATGAGGAGTTAGTAGAATTAGTCCA
TAAGGGAGATAAAATAGCTGAAGAAACACTGATAAAGAGATATAAAAATTTTGTCCTGGCCAAGTCTAGGTCTTATTTTT
TGGTAGGAGCAGATAGAGAAGACATTGTCCAGGAAGGAATGATTGGGTTATATAAAGCTATTAGAGACTATAAGGTTGAT
AGACTGGCTTCTTTCCGGGCTTTTGCTGAATTATGCATTACCCGGCAGATTATTACAGCTATTAAAGCAGCTACCCGCCA
GAAGCATCAGCCGTTAAATTCTTACGTTTCCTTAAACCGCCCTATTTATGATGAAGAATCGGACCGGACCCTGCTGGATG
TTCTAAATGGGGGAAAGCTTTCCGATCCTGAAGAACTTTTTGTGAGCAAGGAAACCTATAATTTAATTGAAAATAAAATT
ACTGAAATGCTTAGCCAGCTGGAATTAAATGTTCTCCAGGAATATCTTGAAGGCAAATCATACCAGGATATAGCCGAAAG
TTTAGACAAGCACGTGAAATCAGTTGATAATGCACTTCAGCGGGTTAAGAGAAAGCTGGAAATTTTCCTGGAGAAACATA
ATATTTAA

Upstream 100 bases:

>100_bases
TTATTTTTGAGGTTGTCCGACAACGGCGCTCAACTTAATACTGCTTGACTATGTTCTATTAAATAAGTATAATAGAGATG
TATAAGGGAGGGGATCCTGG

Downstream 100 bases:

>100_bases
TTTTCATTATGCCACCATCCGGTGGTGCTTTTACATAATGACTGCTATAATTAAAAAGTATTTCTGGCAGCAGTCACTTG
CATTACAAGACTAAAACAAG

Product: RNA polymerase factor sigma-70

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MKGNAQEKYDYEDYNQLGDEELVELVHKGDKIAEETLIKRYKNFVLAKSRSYFLVGADREDIVQEGMIGLYKAIRDYKVD
RLASFRAFAELCITRQIITAIKAATRQKHQPLNSYVSLNRPIYDEESDRTLLDVLNGGKLSDPEELFVSKETYNLIENKI
TEMLSQLELNVLQEYLEGKSYQDIAESLDKHVKSVDNALQRVKRKLEIFLEKHNI

Sequences:

>Translated_215_residues
MKGNAQEKYDYEDYNQLGDEELVELVHKGDKIAEETLIKRYKNFVLAKSRSYFLVGADREDIVQEGMIGLYKAIRDYKVD
RLASFRAFAELCITRQIITAIKAATRQKHQPLNSYVSLNRPIYDEESDRTLLDVLNGGKLSDPEELFVSKETYNLIENKI
TEMLSQLELNVLQEYLEGKSYQDIAESLDKHVKSVDNALQRVKRKLEIFLEKHNI
>Mature_215_residues
MKGNAQEKYDYEDYNQLGDEELVELVHKGDKIAEETLIKRYKNFVLAKSRSYFLVGADREDIVQEGMIGLYKAIRDYKVD
RLASFRAFAELCITRQIITAIKAATRQKHQPLNSYVSLNRPIYDEESDRTLLDVLNGGKLSDPEELFVSKETYNLIENKI
TEMLSQLELNVLQEYLEGKSYQDIAESLDKHVKSVDNALQRVKRKLEIFLEKHNI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in the transition to post-exponential phase in the beginning of sporulation [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=195, Percent_Identity=29.2307692307692, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014218
- InterPro:   IPR016371
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 24985; Mature: 24985

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGNAQEKYDYEDYNQLGDEELVELVHKGDKIAEETLIKRYKNFVLAKSRSYFLVGADRE
CCCCCCCCCCHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCHH
DIVQEGMIGLYKAIRDYKVDRLASFRAFAELCITRQIITAIKAATRQKHQPLNSYVSLNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
PIYDEESDRTLLDVLNGGKLSDPEELFVSKETYNLIENKITEMLSQLELNVLQEYLEGKS
CCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YQDIAESLDKHVKSVDNALQRVKRKLEIFLEKHNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKGNAQEKYDYEDYNQLGDEELVELVHKGDKIAEETLIKRYKNFVLAKSRSYFLVGADRE
CCCCCCCCCCHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCHH
DIVQEGMIGLYKAIRDYKVDRLASFRAFAELCITRQIITAIKAATRQKHQPLNSYVSLNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
PIYDEESDRTLLDVLNGGKLSDPEELFVSKETYNLIENKITEMLSQLELNVLQEYLEGKS
CCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YQDIAESLDKHVKSVDNALQRVKRKLEIFLEKHNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3277943; 6322121 [H]