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The map label for this gene is murE [H]

Identifier: 220930918

GI number: 220930918

Start: 81799

End: 83241

Strand: Reverse

Name: murE [H]

Synonym: Hore_00700

Alternate gene names: 220930918

Gene position: 83241-81799 (Counterclockwise)

Preceding gene: 220930930

Following gene: 220930913

Centisome position: 3.23

GC content: 36.45

Gene sequence:

>1443_bases
ATGGTAATGGAAAGAAAGGAAGACCTCCCTGCCTTTACAGATATAACCTGTGATTCAAGAGAAGTAAAACCGGGATATGC
TTTTGTAGCCATAACGGGGTTCAAAAATGATGGACATAATTTTATTGAAGAAGCTATTAATAAAGGGGCCAGTGTCATAT
TCACGGAAAAGGAAATAAAATCCCGGAGTGGTATTCCCATCGTCCGGGTTAAAGATAGTCGCTCTATACTGGGAAAGCTG
GCAGCTGAATTTTATAATTACCCATCCCGCAAATTAAAACTGATAGGTGTTACCGGAACAAACGGGAAAACAACAACCAC
CCACCTGATTTATCATTTACTTAACTATAAAGGTAAAAATTCAGGGCTAATAGGTACAGTAAAAGTCGATACCGGTAAGA
GAACAATACCCGGAAACTTAACAACACCTGAACCGGTTACCCTACAAAAATATCTTGATGAAATGGTAAAACACAATCTT
AATTATGCCTGTATGGAAGTCTCCTCCCATGGTATCAAATTAAAGAGGATTACCGGTAGTCAATTTGCAATAAAAGTTGG
TACTAACATTACAGTCGACCATTTTGACCTTCATTCAGACTTCTCTGATTATGTCCAGGTAAAAAAATCCTTTCTGGAAG
ATAAAAACCCCCATACCCTTGTTTTATTAAATAATGATGACCATTATTTAAGTTCTTTTGGTAATATCGCTAAAAATCAA
ATTGGGTATGGGATTACCACAAATACAGATATTACAGCAGAAAATATTAATTACAGCGGAATTACTACTGACTTTGTTTA
CTCACTAAATAAATCAATAAAAACCAGCCAGGGTAATAAAATAGGACCGATGAAGGTCCCTATTAATCTTAAAATACCTG
GAGAACATAACATTTATAATGCCCTGGTAGCTATAACAATTGGCCTCTATTATGGTTTAACACCTGAAAAAATCCAGGAC
TTTTTTGGCCAGTTCAGGGGAATCTGGCGGAGATTCCAGGTGATTTACAACAAAGATTTTGCAATTATAGATGACTGTGC
CCATAATCCCGGTAGCTATGGAGCAGTTTTTAAAACAGTAAGCCAGCTCAAATATAAAAATCTATTAATTGTTAATGCTA
TCAGAGGAAACAGAGGAGCCCAGATCAATAAAGGAAATGCAGAAACAATAGGAAAGTGGCTTAAAAAATTTCACAATTAC
TATTTGTTAACCACAAACTGTAATGATGTTGTTAAACCAATTGATAAGGTTAGCCCTGAGGAAGAAAAAGTTTTCCTGCA
GGCCCTGGACAAAAACAATATTAAATATAGCCATCTCGATCAATTAAAACCTGCCTTAAAAAATGTTCTGGATAAGGTTA
CACCCGATGATATAATTCTCCTACTGGGGGCCCATGCTATGGATGAAGCCGGTGACATGATTCTAGAGATGATCAAGGAA
TAA

Upstream 100 bases:

>100_bases
GTTATAATAATAAAGAAAGCGAAATAGTCTTATAGTAAGAAGTAAACTCTTTTAAAAAAATTTTACTTACTTATAAAATA
GATGCTGGGGGTTAATGGCA

Downstream 100 bases:

>100_bases
AAGTATTATTCTTTTCTTTCTGACCCAGTTCTGTTCTATATTTAATCAAGACCTCTTTAACCCCCTGGTAGCAATTCATT
TTGTTTACTCTGTCTTTAAT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-- lysine ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MVMERKEDLPAFTDITCDSREVKPGYAFVAITGFKNDGHNFIEEAINKGASVIFTEKEIKSRSGIPIVRVKDSRSILGKL
AAEFYNYPSRKLKLIGVTGTNGKTTTTHLIYHLLNYKGKNSGLIGTVKVDTGKRTIPGNLTTPEPVTLQKYLDEMVKHNL
NYACMEVSSHGIKLKRITGSQFAIKVGTNITVDHFDLHSDFSDYVQVKKSFLEDKNPHTLVLLNNDDHYLSSFGNIAKNQ
IGYGITTNTDITAENINYSGITTDFVYSLNKSIKTSQGNKIGPMKVPINLKIPGEHNIYNALVAITIGLYYGLTPEKIQD
FFGQFRGIWRRFQVIYNKDFAIIDDCAHNPGSYGAVFKTVSQLKYKNLLIVNAIRGNRGAQINKGNAETIGKWLKKFHNY
YLLTTNCNDVVKPIDKVSPEEEKVFLQALDKNNIKYSHLDQLKPALKNVLDKVTPDDIILLLGAHAMDEAGDMILEMIKE

Sequences:

>Translated_480_residues
MVMERKEDLPAFTDITCDSREVKPGYAFVAITGFKNDGHNFIEEAINKGASVIFTEKEIKSRSGIPIVRVKDSRSILGKL
AAEFYNYPSRKLKLIGVTGTNGKTTTTHLIYHLLNYKGKNSGLIGTVKVDTGKRTIPGNLTTPEPVTLQKYLDEMVKHNL
NYACMEVSSHGIKLKRITGSQFAIKVGTNITVDHFDLHSDFSDYVQVKKSFLEDKNPHTLVLLNNDDHYLSSFGNIAKNQ
IGYGITTNTDITAENINYSGITTDFVYSLNKSIKTSQGNKIGPMKVPINLKIPGEHNIYNALVAITIGLYYGLTPEKIQD
FFGQFRGIWRRFQVIYNKDFAIIDDCAHNPGSYGAVFKTVSQLKYKNLLIVNAIRGNRGAQINKGNAETIGKWLKKFHNY
YLLTTNCNDVVKPIDKVSPEEEKVFLQALDKNNIKYSHLDQLKPALKNVLDKVTPDDIILLLGAHAMDEAGDMILEMIKE
>Mature_480_residues
MVMERKEDLPAFTDITCDSREVKPGYAFVAITGFKNDGHNFIEEAINKGASVIFTEKEIKSRSGIPIVRVKDSRSILGKL
AAEFYNYPSRKLKLIGVTGTNGKTTTTHLIYHLLNYKGKNSGLIGTVKVDTGKRTIPGNLTTPEPVTLQKYLDEMVKHNL
NYACMEVSSHGIKLKRITGSQFAIKVGTNITVDHFDLHSDFSDYVQVKKSFLEDKNPHTLVLLNNDDHYLSSFGNIAKNQ
IGYGITTNTDITAENINYSGITTDFVYSLNKSIKTSQGNKIGPMKVPINLKIPGEHNIYNALVAITIGLYYGLTPEKIQD
FFGQFRGIWRRFQVIYNKDFAIIDDCAHNPGSYGAVFKTVSQLKYKNLLIVNAIRGNRGAQINKGNAETIGKWLKKFHNY
YLLTTNCNDVVKPIDKVSPEEEKVFLQALDKNNIKYSHLDQLKPALKNVLDKVTPDDIILLLGAHAMDEAGDMILEMIKE

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=274, Percent_Identity=33.5766423357664, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1786274, Length=365, Percent_Identity=22.7397260273973, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 53779; Mature: 53779

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS00435 PEROXIDASE_1 ; PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMERKEDLPAFTDITCDSREVKPGYAFVAITGFKNDGHNFIEEAINKGASVIFTEKEIK
CCCCCCCCCCCEEECCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCEEEEEHHHHH
SRSGIPIVRVKDSRSILGKLAAEFYNYPSRKLKLIGVTGTNGKTTTTHLIYHLLNYKGKN
CCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCC
SGLIGTVKVDTGKRTIPGNLTTPEPVTLQKYLDEMVKHNLNYACMEVSSHGIKLKRITGS
CCEEEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCC
QFAIKVGTNITVDHFDLHSDFSDYVQVKKSFLEDKNPHTLVLLNNDDHYLSSFGNIAKNQ
EEEEEECCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHH
IGYGITTNTDITAENINYSGITTDFVYSLNKSIKTSQGNKIGPMKVPINLKIPGEHNIYN
CCCCEECCCCEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCHHH
ALVAITIGLYYGLTPEKIQDFFGQFRGIWRRFQVIYNKDFAIIDDCAHNPGSYGAVFKTV
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHH
SQLKYKNLLIVNAIRGNRGAQINKGNAETIGKWLKKFHNYYLLTTNCNDVVKPIDKVSPE
HHHHHCCEEEEEEEECCCCCEECCCCHHHHHHHHHHHCCEEEEEECCHHHHHHHHHCCCH
EEKVFLQALDKNNIKYSHLDQLKPALKNVLDKVTPDDIILLLGAHAMDEAGDMILEMIKE
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVMERKEDLPAFTDITCDSREVKPGYAFVAITGFKNDGHNFIEEAINKGASVIFTEKEIK
CCCCCCCCCCCEEECCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCEEEEEHHHHH
SRSGIPIVRVKDSRSILGKLAAEFYNYPSRKLKLIGVTGTNGKTTTTHLIYHLLNYKGKN
CCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCC
SGLIGTVKVDTGKRTIPGNLTTPEPVTLQKYLDEMVKHNLNYACMEVSSHGIKLKRITGS
CCEEEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCC
QFAIKVGTNITVDHFDLHSDFSDYVQVKKSFLEDKNPHTLVLLNNDDHYLSSFGNIAKNQ
EEEEEECCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHH
IGYGITTNTDITAENINYSGITTDFVYSLNKSIKTSQGNKIGPMKVPINLKIPGEHNIYN
CCCCEECCCCEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCHHH
ALVAITIGLYYGLTPEKIQDFFGQFRGIWRRFQVIYNKDFAIIDDCAHNPGSYGAVFKTV
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHH
SQLKYKNLLIVNAIRGNRGAQINKGNAETIGKWLKKFHNYYLLTTNCNDVVKPIDKVSPE
HHHHHCCEEEEEEEECCCCCEECCCCHHHHHHHHHHHCCEEEEEECCHHHHHHHHHCCCH
EEKVFLQALDKNNIKYSHLDQLKPALKNVLDKVTPDDIILLLGAHAMDEAGDMILEMIKE
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]