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The map label for this gene is dus1 [H]

Identifier: 220930917

GI number: 220930917

Start: 80808

End: 81809

Strand: Direct

Name: dus1 [H]

Synonym: Hore_00690

Alternate gene names: 220930917

Gene position: 80808-81809 (Clockwise)

Preceding gene: 220930916

Following gene: 220930919

Centisome position: 3.13

GC content: 41.62

Gene sequence:

>1002_bases
ATGAAGATCGGCAACCTGATTGTAGAAAACCCCATTATCCTGGCTCCAATGGCAGGTGTTACTGATTATCCTTTTCGTCA
AATTTCCCGGGAAATGAACTGCCAGCTAATTTATACAGAAATGGTTAGTAGTAAGGGTTTAATATATGGTAATCGTAAGA
CCAGGGATTTAATGGATTTTGATAGAGGGAAAAAAGGACTTATCGGGATTCAGATTTTTGGGGGAGATCCTGAAACCATG
GCCCGGGCAGCGGTACAGATAAAAAATCACTATAGACCGGATTTAATAGACATAAATATGGGTTGCCCAACCCCCAAGAT
AGTTAAAAATGGATCTGGAGCTGCATTAATGAAAGTTCCTGAGCGGGCCAAAAGAGTGATGGAGGCAGTAGCATCAGCTG
TTGACATTCCGGTTACTGTTAAGATACGTATGGGGTGGGACCATAGCCATGTAAATGCTGTAGATATAGCTAAAATTGCC
GAAGAGGTTGGATTGAAGGCGGTTGCTATTCACGGGAGAACCCGGGAGGATTTTTATAGGGGAGAAGCCAACTGGGATAT
AATCAGAGAGGTAAAAAGGGAAGTAACTATACCTGTTATTGGAAATGGGGATGTGTTTACCCCTGAAGATGCTAAAAAGA
TGATGGAAGAGACCGGATGTGATGGAGTTATGGTGGCCCGGGGGGTAAGAGGAAACCCCTGGCTTTTAAAGCGGGCATAT
TATTTAGTAAAAAAGGGGGAACATTTACCCGGTCCCACCTGGGAAGAAAGAATTAATATGGCTATAAAACACCTTGATAT
GGCAGTGGCTTATTATGGAGAAAAAGTTGCTATACCCAGGATGAGGAAACATATTGGCTGGTATCTTAAAGGGTTACCGG
GTAACAGTGATATTAAAGACAGAGTAAACAAAATGAATTGCTACCAGGGGGTTAAAGAGGTCTTGATTAAATATAGAACA
GAACTGGGTCAGAAAGAAAAGAATAATACTTTTATTCCTTGA

Upstream 100 bases:

>100_bases
GCATCTGAAACAGATGAAATTGATAAAGTAGATCCCTTTTTAACCCTTGACGGACTCTACTATATTGCCAAAATAAACGG
AATTGTGGAGTGAATAACTT

Downstream 100 bases:

>100_bases
TCATCTCTAGAATCATGTCACCGGCTTCATCCATAGCATGGGCCCCCAGTAGGAGAATTATATCATCGGGTGTAACCTTA
TCCAGAACATTTTTTAAGGC

Product: putative TIM-barrel protein, nifR3 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKIGNLIVENPIILAPMAGVTDYPFRQISREMNCQLIYTEMVSSKGLIYGNRKTRDLMDFDRGKKGLIGIQIFGGDPETM
ARAAVQIKNHYRPDLIDINMGCPTPKIVKNGSGAALMKVPERAKRVMEAVASAVDIPVTVKIRMGWDHSHVNAVDIAKIA
EEVGLKAVAIHGRTREDFYRGEANWDIIREVKREVTIPVIGNGDVFTPEDAKKMMEETGCDGVMVARGVRGNPWLLKRAY
YLVKKGEHLPGPTWEERINMAIKHLDMAVAYYGEKVAIPRMRKHIGWYLKGLPGNSDIKDRVNKMNCYQGVKEVLIKYRT
ELGQKEKNNTFIP

Sequences:

>Translated_333_residues
MKIGNLIVENPIILAPMAGVTDYPFRQISREMNCQLIYTEMVSSKGLIYGNRKTRDLMDFDRGKKGLIGIQIFGGDPETM
ARAAVQIKNHYRPDLIDINMGCPTPKIVKNGSGAALMKVPERAKRVMEAVASAVDIPVTVKIRMGWDHSHVNAVDIAKIA
EEVGLKAVAIHGRTREDFYRGEANWDIIREVKREVTIPVIGNGDVFTPEDAKKMMEETGCDGVMVARGVRGNPWLLKRAY
YLVKKGEHLPGPTWEERINMAIKHLDMAVAYYGEKVAIPRMRKHIGWYLKGLPGNSDIKDRVNKMNCYQGVKEVLIKYRT
ELGQKEKNNTFIP
>Mature_333_residues
MKIGNLIVENPIILAPMAGVTDYPFRQISREMNCQLIYTEMVSSKGLIYGNRKTRDLMDFDRGKKGLIGIQIFGGDPETM
ARAAVQIKNHYRPDLIDINMGCPTPKIVKNGSGAALMKVPERAKRVMEAVASAVDIPVTVKIRMGWDHSHVNAVDIAKIA
EEVGLKAVAIHGRTREDFYRGEANWDIIREVKREVTIPVIGNGDVFTPEDAKKMMEETGCDGVMVARGVRGNPWLLKRAY
YLVKKGEHLPGPTWEERINMAIKHLDMAVAYYGEKVAIPRMRKHIGWYLKGLPGNSDIKDRVNKMNCYQGVKEVLIKYRT
ELGQKEKNNTFIP

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=290, Percent_Identity=28.2758620689655, Blast_Score=134, Evalue=8e-32,
Organism=Homo sapiens, GI239788462, Length=293, Percent_Identity=28.3276450511945, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI31742496, Length=227, Percent_Identity=32.5991189427313, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI40807366, Length=230, Percent_Identity=31.304347826087, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI8923374, Length=250, Percent_Identity=28, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1789660, Length=318, Percent_Identity=38.0503144654088, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI1788462, Length=239, Percent_Identity=33.0543933054393, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI145693211, Length=241, Percent_Identity=28.6307053941909, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17543114, Length=287, Percent_Identity=27.1777003484321, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25144369, Length=248, Percent_Identity=29.0322580645161, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17510279, Length=246, Percent_Identity=29.6747967479675, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17507177, Length=231, Percent_Identity=26.8398268398268, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323433, Length=303, Percent_Identity=26.7326732673267, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323560, Length=304, Percent_Identity=27.6315789473684, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323437, Length=230, Percent_Identity=25.6521739130435, Blast_Score=90, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24585320, Length=266, Percent_Identity=29.6992481203008, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI45549423, Length=239, Percent_Identity=32.2175732217573, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24580595, Length=227, Percent_Identity=29.9559471365639, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI19920448, Length=227, Percent_Identity=29.9559471365639, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI19921524, Length=232, Percent_Identity=29.7413793103448, Blast_Score=102, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37523; Mature: 37523

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGNLIVENPIILAPMAGVTDYPFRQISREMNCQLIYTEMVSSKGLIYGNRKTRDLMDF
CCCCCEEECCCEEEEECCCCCCCCHHHHHHHCCCEEEEEEHHCCCCEEECCCCCHHHHHH
DRGKKGLIGIQIFGGDPETMARAAVQIKNHYRPDLIDINMGCPTPKIVKNGSGAALMKVP
CCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHEECCCCCEEECCH
ERAKRVMEAVASAVDIPVTVKIRMGWDHSHVNAVDIAKIAEEVGLKAVAIHGRTREDFYR
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEECCCCHHHHHC
GEANWDIIREVKREVTIPVIGNGDVFTPEDAKKMMEETGCDGVMVARGVRGNPWLLKRAY
CCCCHHHHHHHHHHEEEEEECCCCEECCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHH
YLVKKGEHLPGPTWEERINMAIKHLDMAVAYYGEKVAIPRMRKHIGWYLKGLPGNSDIKD
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHCCEEECCCCCCHHHH
RVNKMNCYQGVKEVLIKYRTELGQKEKNNTFIP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKIGNLIVENPIILAPMAGVTDYPFRQISREMNCQLIYTEMVSSKGLIYGNRKTRDLMDF
CCCCCEEECCCEEEEECCCCCCCCHHHHHHHCCCEEEEEEHHCCCCEEECCCCCHHHHHH
DRGKKGLIGIQIFGGDPETMARAAVQIKNHYRPDLIDINMGCPTPKIVKNGSGAALMKVP
CCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHEECCCCCEEECCH
ERAKRVMEAVASAVDIPVTVKIRMGWDHSHVNAVDIAKIAEEVGLKAVAIHGRTREDFYR
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEECCCCHHHHHC
GEANWDIIREVKREVTIPVIGNGDVFTPEDAKKMMEETGCDGVMVARGVRGNPWLLKRAY
CCCCHHHHHHHHHHEEEEEECCCCEECCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHH
YLVKKGEHLPGPTWEERINMAIKHLDMAVAYYGEKVAIPRMRKHIGWYLKGLPGNSDIKD
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHCCEEECCCCCCHHHH
RVNKMNCYQGVKEVLIKYRTELGQKEKNNTFIP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]