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The map label for this gene is dnaN [H]

Identifier: 220930852

GI number: 220930852

Start: 2332

End: 3435

Strand: Direct

Name: dnaN [H]

Synonym: Hore_00020

Alternate gene names: 220930852

Gene position: 2332-3435 (Clockwise)

Preceding gene: 220930851

Following gene: 220930853

Centisome position: 0.09

GC content: 37.05

Gene sequence:

>1104_bases
ATGGAATTTACAATTTCCCAGAAAGATTTTTCCAGGAGCATAAATATAGTCCAGCGCGCGGTTTCTTCTAAAAATACTAT
GCCCATCCTCACCGGAATTTACCTTGAAGCCAGAAAAGATAAAGGACTACACATGAAGGCAACAGATCTGGAATTAGGGA
TAGAATACTGGGTTAAAGCAGATATTATAACTGAAGGGAAAATAGTTTTACCAGCCAGTCATCTCAGTGGTATTATCAGG
GAATTACCTGATGACAATATTAACTTTAAAGCTGACCTTGATAAATATGAGGCTGAATTGACCTGTTTAAGTTCCAGGTT
TAAAATTAAGGGATTTGATGCTGATGAATTTCCCCAGTTACCTGAGGTTAACATTCCCCACAAAATTACTTTAAATTGTG
GTGACCTCAATAGATTAATAGATGAAGTTAAATTTTCAACTTCAACCGACCAGACTCAACCAGCTTTAACCGGGGGTTCT
ATGGTTATTGATAGTGGTAAAATAAGGATGGTTGCTACCAATACATACCGGCTATCCTTATCTTCACTGGATCTCGATGG
TAGCCCTGAAGGAAAGAAAGAAGTAATTTTACCGGGCAATACTTTAAGTGAATTAAGTCATCTCCTTGATGATGATGGAG
AAGTCGAAATTTTAATTGGGGATAACTATGTCAGGTTTAATTTTAATGGTATAGTCCTGGTATCCCGGGTTATAGAAGGG
CAGTTTCCCAATTACCAGCAGGTTTTACCAAAAGAATATAACACTAAAGTCAGGGTTGATCGTAATAATTTATTACGGTC
AGTAAGAAGGGTGTCCCTGATTGCACGGCTTGATTCCAATGTAATTTCTATTTCAGTTAAGGATAACAAAATGATTATTA
CATCCCAGGATTCAGAAGAGGGTTTTGCCCGGGAAGAAATTGAAATAGAAAGCCAGGGTCCTGAACAGAATATTAATATA
GATGCCGGTTATTTACTTGATGTCTTAAAGGTACTGAATGATGATGTGGTTATTCTGGAACTTATTGGACCTTTAAATCC
TTTAACTTTAAAGAAAGAAAATAGTGATGATTTTATTTACCTGGTAATGCCCGTCCGCCGGTAA

Upstream 100 bases:

>100_bases
CTACAGGTATTACTACTATAAGTACTGGTTTAAATAAATAAATTAAATTATAATTTTAATCTTTTTTTCTGTGGACAACT
TTCGAAATGGAGGGTATGTT

Downstream 100 bases:

>100_bases
ACGGGAGGTTATAAAATGAAAGATATAAAGATTAATACTGAAAAAATAAAACTGGATCAATTACTTAAATATGCCAATAT
TGTGGCCACTGGAGGACAGG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MEFTISQKDFSRSINIVQRAVSSKNTMPILTGIYLEARKDKGLHMKATDLELGIEYWVKADIITEGKIVLPASHLSGIIR
ELPDDNINFKADLDKYEAELTCLSSRFKIKGFDADEFPQLPEVNIPHKITLNCGDLNRLIDEVKFSTSTDQTQPALTGGS
MVIDSGKIRMVATNTYRLSLSSLDLDGSPEGKKEVILPGNTLSELSHLLDDDGEVEILIGDNYVRFNFNGIVLVSRVIEG
QFPNYQQVLPKEYNTKVRVDRNNLLRSVRRVSLIARLDSNVISISVKDNKMIITSQDSEEGFAREEIEIESQGPEQNINI
DAGYLLDVLKVLNDDVVILELIGPLNPLTLKKENSDDFIYLVMPVRR

Sequences:

>Translated_367_residues
MEFTISQKDFSRSINIVQRAVSSKNTMPILTGIYLEARKDKGLHMKATDLELGIEYWVKADIITEGKIVLPASHLSGIIR
ELPDDNINFKADLDKYEAELTCLSSRFKIKGFDADEFPQLPEVNIPHKITLNCGDLNRLIDEVKFSTSTDQTQPALTGGS
MVIDSGKIRMVATNTYRLSLSSLDLDGSPEGKKEVILPGNTLSELSHLLDDDGEVEILIGDNYVRFNFNGIVLVSRVIEG
QFPNYQQVLPKEYNTKVRVDRNNLLRSVRRVSLIARLDSNVISISVKDNKMIITSQDSEEGFAREEIEIESQGPEQNINI
DAGYLLDVLKVLNDDVVILELIGPLNPLTLKKENSDDFIYLVMPVRR
>Mature_367_residues
MEFTISQKDFSRSINIVQRAVSSKNTMPILTGIYLEARKDKGLHMKATDLELGIEYWVKADIITEGKIVLPASHLSGIIR
ELPDDNINFKADLDKYEAELTCLSSRFKIKGFDADEFPQLPEVNIPHKITLNCGDLNRLIDEVKFSTSTDQTQPALTGGS
MVIDSGKIRMVATNTYRLSLSSLDLDGSPEGKKEVILPGNTLSELSHLLDDDGEVEILIGDNYVRFNFNGIVLVSRVIEG
QFPNYQQVLPKEYNTKVRVDRNNLLRSVRRVSLIARLDSNVISISVKDNKMIITSQDSEEGFAREEIEIESQGPEQNINI
DAGYLLDVLKVLNDDVVILELIGPLNPLTLKKENSDDFIYLVMPVRR

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=370, Percent_Identity=25.6756756756757, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41214; Mature: 41214

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFTISQKDFSRSINIVQRAVSSKNTMPILTGIYLEARKDKGLHMKATDLELGIEYWVKA
CCEEECHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCEEEECCCEECEEEEEEE
DIITEGKIVLPASHLSGIIRELPDDNINFKADLDKYEAELTCLSSRFKIKGFDADEFPQL
EEEECCEEEEEHHHHHHHHHHCCCCCCEEEECCHHHHHEEEEECCCEEEECCCHHHCCCC
PEVNIPHKITLNCGDLNRLIDEVKFSTSTDQTQPALTGGSMVIDSGKIRMVATNTYRLSL
CCCCCCEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCEEEEEECEEEEEE
SSLDLDGSPEGKKEVILPGNTLSELSHLLDDDGEVEILIGDNYVRFNFNGIVLVSRVIEG
EEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCCEEEEECCCEEEEECCCEEEEEEHHCC
QFPNYQQVLPKEYNTKVRVDRNNLLRSVRRVSLIARLDSNVISISVKDNKMIITSQDSEE
CCCCHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEECCCCC
GFAREEIEIESQGPEQNINIDAGYLLDVLKVLNDDVVILELIGPLNPLTLKKENSDDFIY
CCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCEEE
LVMPVRR
EEEECCC
>Mature Secondary Structure
MEFTISQKDFSRSINIVQRAVSSKNTMPILTGIYLEARKDKGLHMKATDLELGIEYWVKA
CCEEECHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCEEEECCCEECEEEEEEE
DIITEGKIVLPASHLSGIIRELPDDNINFKADLDKYEAELTCLSSRFKIKGFDADEFPQL
EEEECCEEEEEHHHHHHHHHHCCCCCCEEEECCHHHHHEEEEECCCEEEECCCHHHCCCC
PEVNIPHKITLNCGDLNRLIDEVKFSTSTDQTQPALTGGSMVIDSGKIRMVATNTYRLSL
CCCCCCEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCEEEEEECEEEEEE
SSLDLDGSPEGKKEVILPGNTLSELSHLLDDDGEVEILIGDNYVRFNFNGIVLVSRVIEG
EEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCCEEEEECCCEEEEECCCEEEEEEHHCC
QFPNYQQVLPKEYNTKVRVDRNNLLRSVRRVSLIARLDSNVISISVKDNKMIITSQDSEE
CCCCHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEECCCCC
GFAREEIEIESQGPEQNINIDAGYLLDVLKVLNDDVVILELIGPLNPLTLKKENSDDFIY
CCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCEEE
LVMPVRR
EEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA