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The map label for this gene is nth [H]

Identifier: 221230710

GI number: 221230710

Start: 2725648

End: 2726409

Strand: Reverse

Name: nth [H]

Synonym: MLBr_02301

Alternate gene names: 221230710

Gene position: 2726409-2725648 (Counterclockwise)

Preceding gene: 221230712

Following gene: 221230709

Centisome position: 83.43

GC content: 59.97

Gene sequence:

>762_bases
ATGCCCCTCACGCCTGGTGTCGACGTGGCCCGGCGCTGGTCCGGGGAAACCAGACTGGGTTTGGTGCGACGGGCGCGGAG
GATGAATCGTGCATTGGCGCAAGCATTTCCGCATGTGTACTGTGAATTGGATTTCACGTCGCCGCTGGAGTTGACGGTGG
CCACCATCCTTTCGGCGCAGAGCACCGATAAGCGGGTGAACTTGACGACACCAGCTGTGTTTGCGCGTTACCGGTCGGCG
CTGGACTACATGCAAGCGGATCGCGCTGAACTAGAAAACTTCATACGTCCTACGGGTTTCTTCCGTAACAAGGCGGCTTC
GCTTATCAGGCTCGGGCAGGCCTTGGTCGAGCGGTTCGATGGCGAGGTGCCCTCGACCATGGTTGACCTGTTTACGTTAC
CCGGTGTAGGACGCAAGACCGCTAATGTCATTCTGGGAAATGCGTTCGGTATCCCCGGGATCACTGTCGACACGCATTTT
GGACGATTAGTGCGGCGATGGCGTTGGACGGCCGAAGAGGATCCAGTCAAGGTGGAGCATGCTGTCGGTGAACTGATCGA
ACGCGATCAGTGGACTTTGCTGAGCCACCGAGTGATCTTCCACGGTCGTCGGGTGTGCCACGCGCGCAAACCGGCATGCG
GTGTTTGCGTACTTGCCAAGGACTGTCCCTCCTTCGGCCTTGGCCCCACTGAACCGCTGCTGGCCGCGCCTCTCGTCCAA
GGCCCGGAAGCCGGGCACTTGCTGGCCCTGGCTGGACTATAA

Upstream 100 bases:

>100_bases
ACACAAGGAGAGTAAACCTGGCTCAGGTCTCAGTGGGATTACGAAATTCGGTACTCTGTCGTGGGTGACAGCAGCAAAGT
TATACGGGCGTTCTAAAGTA

Downstream 100 bases:

>100_bases
GTTCAGGTTGTAGATAACGGACCGTCCAACTCTGCCGCGTTGGGACCGCAATGAAGACCTTAACCCCGAAAACCTGTGGG
ACCATCGTAGTACTTGCAGT

Product: putative endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MPLTPGVDVARRWSGETRLGLVRRARRMNRALAQAFPHVYCELDFTSPLELTVATILSAQSTDKRVNLTTPAVFARYRSA
LDYMQADRAELENFIRPTGFFRNKAASLIRLGQALVERFDGEVPSTMVDLFTLPGVGRKTANVILGNAFGIPGITVDTHF
GRLVRRWRWTAEEDPVKVEHAVGELIERDQWTLLSHRVIFHGRRVCHARKPACGVCVLAKDCPSFGLGPTEPLLAAPLVQ
GPEAGHLLALAGL

Sequences:

>Translated_253_residues
MPLTPGVDVARRWSGETRLGLVRRARRMNRALAQAFPHVYCELDFTSPLELTVATILSAQSTDKRVNLTTPAVFARYRSA
LDYMQADRAELENFIRPTGFFRNKAASLIRLGQALVERFDGEVPSTMVDLFTLPGVGRKTANVILGNAFGIPGITVDTHF
GRLVRRWRWTAEEDPVKVEHAVGELIERDQWTLLSHRVIFHGRRVCHARKPACGVCVLAKDCPSFGLGPTEPLLAAPLVQ
GPEAGHLLALAGL
>Mature_252_residues
PLTPGVDVARRWSGETRLGLVRRARRMNRALAQAFPHVYCELDFTSPLELTVATILSAQSTDKRVNLTTPAVFARYRSAL
DYMQADRAELENFIRPTGFFRNKAASLIRLGQALVERFDGEVPSTMVDLFTLPGVGRKTANVILGNAFGIPGITVDTHFG
RLVRRWRWTAEEDPVKVEHAVGELIERDQWTLLSHRVIFHGRRVCHARKPACGVCVLAKDCPSFGLGPTEPLLAAPLVQG
PEAGHLLALAGL

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=178, Percent_Identity=29.7752808988764, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1787920, Length=205, Percent_Identity=36.5853658536585, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17554540, Length=181, Percent_Identity=30.939226519337, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6324530, Length=190, Percent_Identity=31.0526315789474, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319304, Length=195, Percent_Identity=26.1538461538462, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45550361, Length=175, Percent_Identity=30.8571428571429, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 27974; Mature: 27843

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTPGVDVARRWSGETRLGLVRRARRMNRALAQAFPHVYCELDFTSPLELTVATILSAQ
CCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHC
STDKRVNLTTPAVFARYRSALDYMQADRAELENFIRPTGFFRNKAASLIRLGQALVERFD
CCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
GEVPSTMVDLFTLPGVGRKTANVILGNAFGIPGITVDTHFGRLVRRWRWTAEEDPVKVEH
CCCCHHHHHHHHCCCCCCHHHHEEEECCCCCCCCEECHHHHHHHHHHCCCCCCCCCHHHH
AVGELIERDQWTLLSHRVIFHGRRVCHARKPACGVCVLAKDCPSFGLGPTEPLLAAPLVQ
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHCCC
GPEAGHLLALAGL
CCCCCCEEEECCC
>Mature Secondary Structure 
PLTPGVDVARRWSGETRLGLVRRARRMNRALAQAFPHVYCELDFTSPLELTVATILSAQ
CCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHC
STDKRVNLTTPAVFARYRSALDYMQADRAELENFIRPTGFFRNKAASLIRLGQALVERFD
CCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
GEVPSTMVDLFTLPGVGRKTANVILGNAFGIPGITVDTHFGRLVRRWRWTAEEDPVKVEH
CCCCHHHHHHHHCCCCCCHHHHEEEECCCCCCCCEECHHHHHHHHHHCCCCCCCCCHHHH
AVGELIERDQWTLLSHRVIFHGRRVCHARKPACGVCVLAKDCPSFGLGPTEPLLAAPLVQ
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHCCC
GPEAGHLLALAGL
CCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]