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The map label for this gene is gloB [H]

Identifier: 221230712

GI number: 221230712

Start: 2727587

End: 2728375

Strand: Reverse

Name: gloB [H]

Synonym: MLBr_02303

Alternate gene names: 221230712

Gene position: 2728375-2727587 (Counterclockwise)

Preceding gene: 221230713

Following gene: 221230710

Centisome position: 83.49

GC content: 61.09

Gene sequence:

>789_bases
GTGGCCGAGGTGGTCAAGTGGCTAGCCCATCCCGCATACGGCCGGCTGCGGGCGGTCACCGACACAGCGTCGGTTCTGTT
GGCTGATAACCCTGGTTTGTTGACGTTGGAGGGCACCAACACTTGGGTGTTGCGTGGCCCGGGCAGCGGTGAGTTAGTCA
TTGTTGATCCCGGGCCAGGTGATGAACACATTGTACAGGTTGCTACGTTGGGTCGCATCGCGTTGGTGTTGATTAGCCAC
CGGCACCCCGACCATACTGACGGCATCGACAAGTTGGTCGAGCTGACCGGCGCACCGGTGTGCGCCGCAGACCCGCAGTT
TTTACGGGGCGATGGGGTGACATTGACCGACCGTGAGGTGATCGATGCTGGTGGGTTGACGATTACCGTGTTGGCTACCC
CGGGCCACACCGCCGATTCTATGTCGTTCGTCCTCGCTGACGCCGTGCTGACCGCCGATAGTGTGTTGGGCCACGGTACC
ACCGTGATCGATCCCGACGACGGCAGCCTGGCGGACTACTTGGAATCACTACATCGGCTAAGCGGCCTGGGCCGGCGAAC
GGTCTTGCCTGGACACGGGCCGGACTTGGCTAACCTGGAATCGGTCGCGTCAGGGTACCTGGTGCATCGGCAAGAGCGGC
TGGAGCAGGTGCGCGCTGCGTTACGGGAACTTGGTGACGACGCGACTGCACGGCAGGTCGTTGAACATGTTTATGTCGAC
GTCGACGAGAAGTTCTGGGATGCGGCCGAATGGTCTGTTCAAGCGCAATTGGATTATCTGCGCCGCTAA

Upstream 100 bases:

>100_bases
GTGATAGAGGTAGGCATGCGCGTTCGGCAGTCGGTGTGGCAGAGCTACCGCTGGGTGCACCAGTGGAAATTGAGTTGATC
GTGGAACTAGGCTAGCTTCC

Downstream 100 bases:

>100_bases
CACTCGCCGAGATTGATGTTGTCGTGGAAAAATGTCGAGAAAGCTTGTCGGCAAGTCGATTTCGATGTTAACGGCGCTTA
CCTCGCTCGTCGGGCGAGGC

Product: putative hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MAEVVKWLAHPAYGRLRAVTDTASVLLADNPGLLTLEGTNTWVLRGPGSGELVIVDPGPGDEHIVQVATLGRIALVLISH
RHPDHTDGIDKLVELTGAPVCAADPQFLRGDGVTLTDREVIDAGGLTITVLATPGHTADSMSFVLADAVLTADSVLGHGT
TVIDPDDGSLADYLESLHRLSGLGRRTVLPGHGPDLANLESVASGYLVHRQERLEQVRAALRELGDDATARQVVEHVYVD
VDEKFWDAAEWSVQAQLDYLRR

Sequences:

>Translated_262_residues
MAEVVKWLAHPAYGRLRAVTDTASVLLADNPGLLTLEGTNTWVLRGPGSGELVIVDPGPGDEHIVQVATLGRIALVLISH
RHPDHTDGIDKLVELTGAPVCAADPQFLRGDGVTLTDREVIDAGGLTITVLATPGHTADSMSFVLADAVLTADSVLGHGT
TVIDPDDGSLADYLESLHRLSGLGRRTVLPGHGPDLANLESVASGYLVHRQERLEQVRAALRELGDDATARQVVEHVYVD
VDEKFWDAAEWSVQAQLDYLRR
>Mature_261_residues
AEVVKWLAHPAYGRLRAVTDTASVLLADNPGLLTLEGTNTWVLRGPGSGELVIVDPGPGDEHIVQVATLGRIALVLISHR
HPDHTDGIDKLVELTGAPVCAADPQFLRGDGVTLTDREVIDAGGLTITVLATPGHTADSMSFVLADAVLTADSVLGHGTT
VIDPDDGSLADYLESLHRLSGLGRRTVLPGHGPDLANLESVASGYLVHRQERLEQVRAALRELGDDATARQVVEHVYVDV
DEKFWDAAEWSVQAQLDYLRR

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI7705793, Length=256, Percent_Identity=31.640625, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI193205216, Length=240, Percent_Identity=31.6666666666667, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17537503, Length=240, Percent_Identity=31.6666666666667, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI20129357, Length=278, Percent_Identity=29.8561151079137, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28076; Mature: 27945

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVVKWLAHPAYGRLRAVTDTASVLLADNPGLLTLEGTNTWVLRGPGSGELVIVDPGPG
CHHHHHHHHCCCCCCEEEEECCEEEEEECCCCEEEEECCCEEEEECCCCCCEEEECCCCC
DEHIVQVATLGRIALVLISHRHPDHTDGIDKLVELTGAPVCAADPQFLRGDGVTLTDREV
HHHEEEHHHHHHEEEEEEECCCCCCCHHHHHHHHHCCCCEECCCCCEECCCCCEECCCCE
IDAGGLTITVLATPGHTADSMSFVLADAVLTADSVLGHGTTVIDPDDGSLADYLESLHRL
EECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHH
SGLGRRTVLPGHGPDLANLESVASGYLVHRQERLEQVRAALRELGDDATARQVVEHVYVD
HCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
VDEKFWDAAEWSVQAQLDYLRR
CCHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEVVKWLAHPAYGRLRAVTDTASVLLADNPGLLTLEGTNTWVLRGPGSGELVIVDPGPG
HHHHHHHHCCCCCCEEEEECCEEEEEECCCCEEEEECCCEEEEECCCCCCEEEECCCCC
DEHIVQVATLGRIALVLISHRHPDHTDGIDKLVELTGAPVCAADPQFLRGDGVTLTDREV
HHHEEEHHHHHHEEEEEEECCCCCCCHHHHHHHHHCCCCEECCCCCEECCCCCEECCCCE
IDAGGLTITVLATPGHTADSMSFVLADAVLTADSVLGHGTTVIDPDDGSLADYLESLHRL
EECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHH
SGLGRRTVLPGHGPDLANLESVASGYLVHRQERLEQVRAALRELGDDATARQVVEHVYVD
HCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
VDEKFWDAAEWSVQAQLDYLRR
CCHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA