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The map label for this gene is dus

Identifier: 221230646

GI number: 221230646

Start: 2600201

End: 2601355

Strand: Direct

Name: dus

Synonym: MLBr_02186

Alternate gene names: 221230646

Gene position: 2600201-2601355 (Clockwise)

Preceding gene: 221230645

Following gene: 221230647

Centisome position: 79.56

GC content: 62.94

Gene sequence:

>1155_bases
GTGTTGGCTCCGATGGCCGGAGTAACGAACGTCACATTCCGGACACTGTGCCGTGAATTTGGTTCAACGCGTGGCAACCT
GCTGCGCCCAGCTCCACCGCACTTGCGATCGCCACTAGAACAATCGCAGGTAGGCACCATCAGCGGACTGTACGTCTGCG
AAATGGTGACCGCGCGTGCACTAGTCGAGCGAAATGCAGTCACGATACACATGACCACGTTCGCACCGGACGAGTCGCCC
CGGTCGTTGCAGCTCTACACCGTGGACCCGGCTACCACGTACACGGCCGCCAAGATGGTCGCTGACGAAGGTTTGGCCGA
TCACATCGATATGAATTTCGGCTGCCCGATGCCCAAGGTCACCAGACGCGGCGGCGGGGCAGCGCTGCCATACAAACGGC
GACTGTTTGGCCAGATCGTCGCTGCCGCTGTGCGTGCTACCGAAGGCACCAAAATACCGGTGACCGTCAAGTTCCGCATC
GGCATCGACGACGAGCATCACACCCACCTAGATGCCGGCCGTATCGCCGAAGCTGAGGGCGCAGCCGCGGTCGCATTGCA
CGCCCGGACGGCGGCTCAACGCTACTCCGGCACCGCTGATTGGGCACAGATCGCCCAACTCAAACAGCAGGTAAGGACGA
TTCCTGTGCTCGGCAATGGGGACATCTACGACGCGAGCGACGCGCTGGCCATGATGGCCGTCACTGGGTGCGACGGCGTG
GTCATCGGCCGCGGCTGCCTGGGCCGTCCGTGGCTGTTCGCCGAGCTGTCGGCCGCGTTCACCGGCAGACCTGCCCCCGC
CCCGCCCACACTCGGCGAAGTCGCCGAAATCGTCCGTCGGCACGGCAAACTGCTAGTCGCCCATTTCGGCGAAGACAAAG
GCATGCGCGACATCCGCAAACACATCGCGTGGTACCTGCACGGCTTTCCCGCCGGATCCGAATTGCGAAACGCACTGGCA
CTGGTCAAAACGCTCGATGAACTTGACTGCCTATTAAACCGGTTGGACGGAGCTGTTCCGTTCCCGGATGCCGCGATCGG
ACCCCGGGGTAGGCAGGGTTCACCCGCCTGGGTGGCATTGCCCGACAGCTGGCTGACTGACCCTGACGACTGCACAGTGC
CGATCGGCGCCGACATCATGGAATCTGGTGGCTGA

Upstream 100 bases:

>100_bases
ATCACCGCACGCAAGGTGCTATCTACCGAAGGCACGCTGAGGATGAGTGGGCGGTAGTTGACCATTGCGCATCGGCCCGA
TAGAGCTTACCAGCCCGGTT

Downstream 100 bases:

>100_bases
ATCGAAACTCTGCCAAAACCGCGTCTCGATCGATAATTCAGTACCATATGCCCTTAAGTGGCAGTTGTAGCGCTGATAAT
ACCAGCGGTGCATTGACCAT

Product: transcriptional regulator (NifR3/Smm1 family)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MLAPMAGVTNVTFRTLCREFGSTRGNLLRPAPPHLRSPLEQSQVGTISGLYVCEMVTARALVERNAVTIHMTTFAPDESP
RSLQLYTVDPATTYTAAKMVADEGLADHIDMNFGCPMPKVTRRGGGAALPYKRRLFGQIVAAAVRATEGTKIPVTVKFRI
GIDDEHHTHLDAGRIAEAEGAAAVALHARTAAQRYSGTADWAQIAQLKQQVRTIPVLGNGDIYDASDALAMMAVTGCDGV
VIGRGCLGRPWLFAELSAAFTGRPAPAPPTLGEVAEIVRRHGKLLVAHFGEDKGMRDIRKHIAWYLHGFPAGSELRNALA
LVKTLDELDCLLNRLDGAVPFPDAAIGPRGRQGSPAWVALPDSWLTDPDDCTVPIGADIMESGG

Sequences:

>Translated_384_residues
MLAPMAGVTNVTFRTLCREFGSTRGNLLRPAPPHLRSPLEQSQVGTISGLYVCEMVTARALVERNAVTIHMTTFAPDESP
RSLQLYTVDPATTYTAAKMVADEGLADHIDMNFGCPMPKVTRRGGGAALPYKRRLFGQIVAAAVRATEGTKIPVTVKFRI
GIDDEHHTHLDAGRIAEAEGAAAVALHARTAAQRYSGTADWAQIAQLKQQVRTIPVLGNGDIYDASDALAMMAVTGCDGV
VIGRGCLGRPWLFAELSAAFTGRPAPAPPTLGEVAEIVRRHGKLLVAHFGEDKGMRDIRKHIAWYLHGFPAGSELRNALA
LVKTLDELDCLLNRLDGAVPFPDAAIGPRGRQGSPAWVALPDSWLTDPDDCTVPIGADIMESGG
>Mature_384_residues
MLAPMAGVTNVTFRTLCREFGSTRGNLLRPAPPHLRSPLEQSQVGTISGLYVCEMVTARALVERNAVTIHMTTFAPDESP
RSLQLYTVDPATTYTAAKMVADEGLADHIDMNFGCPMPKVTRRGGGAALPYKRRLFGQIVAAAVRATEGTKIPVTVKFRI
GIDDEHHTHLDAGRIAEAEGAAAVALHARTAAQRYSGTADWAQIAQLKQQVRTIPVLGNGDIYDASDALAMMAVTGCDGV
VIGRGCLGRPWLFAELSAAFTGRPAPAPPTLGEVAEIVRRHGKLLVAHFGEDKGMRDIRKHIAWYLHGFPAGSELRNALA
LVKTLDELDCLLNRLDGAVPFPDAAIGPRGRQGSPAWVALPDSWLTDPDDCTVPIGADIMESGG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family

Homologues:

Organism=Homo sapiens, GI239788483, Length=308, Percent_Identity=28.5714285714286, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI239788462, Length=308, Percent_Identity=28.5714285714286, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI40807366, Length=383, Percent_Identity=26.1096605744125, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI31742496, Length=253, Percent_Identity=29.6442687747036, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1789660, Length=326, Percent_Identity=32.8220858895706, Blast_Score=158, Evalue=7e-40,
Organism=Escherichia coli, GI1788462, Length=143, Percent_Identity=39.1608391608392, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI145693211, Length=283, Percent_Identity=27.208480565371, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17543114, Length=313, Percent_Identity=24.9201277955272, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=220, Percent_Identity=30.4545454545455, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17507177, Length=261, Percent_Identity=24.904214559387, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323433, Length=225, Percent_Identity=25.7777777777778, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323437, Length=258, Percent_Identity=27.906976744186, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24585320, Length=305, Percent_Identity=27.5409836065574, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI19921524, Length=239, Percent_Identity=28.4518828451883, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24580595, Length=325, Percent_Identity=25.8461538461538, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19920448, Length=325, Percent_Identity=25.8461538461538, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUS_MYCLE (Q50049)

Other databases:

- EMBL:   U15182
- EMBL:   AL583924
- PIR:   E87182
- RefSeq:   NP_302432.1
- ProteinModelPortal:   Q50049
- SMR:   Q50049
- EnsemblBacteria:   EBMYCT00000027918
- GeneID:   909337
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML2186
- NMPDR:   fig|272631.1.peg.1304
- Leproma:   ML2186
- GeneTree:   EBGT00050000017379
- HOGENOM:   HBG557545
- OMA:   GIDDDHL
- ProtClustDB:   CLSK2751572
- BioCyc:   MLEP272631:ML2186-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11082
- PIRSF:   PIRSF006621
- TIGRFAMs:   TIGR00737

Pfam domain/function: PF01207 Dus

EC number: NA

Molecular weight: Translated: 41101; Mature: 41101

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAPMAGVTNVTFRTLCREFGSTRGNLLRPAPPHLRSPLEQSQVGTISGLYVCEMVTARA
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHCCCHHHHHHHHHHHHHH
LVERNAVTIHMTTFAPDESPRSLQLYTVDPATTYTAAKMVADEGLADHIDMNFGCPMPKV
HHHCCCEEEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHCCCHHHCCCCCCCCCCHH
TRRGGGAALPYKRRLFGQIVAAAVRATEGTKIPVTVKFRIGIDDEHHTHLDAGRIAEAEG
HCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCC
AAAVALHARTAAQRYSGTADWAQIAQLKQQVRTIPVLGNGDIYDASDALAMMAVTGCDGV
CEEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCHHEEEEEEECCCCE
VIGRGCLGRPWLFAELSAAFTGRPAPAPPTLGEVAEIVRRHGKLLVAHFGEDKGMRDIRK
EECCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
HIAWYLHGFPAGSELRNALALVKTLDELDCLLNRLDGAVPFPDAAIGPRGRQGSPAWVAL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEC
PDSWLTDPDDCTVPIGADIMESGG
CCCCCCCCCCCEECCCCHHHCCCC
>Mature Secondary Structure
MLAPMAGVTNVTFRTLCREFGSTRGNLLRPAPPHLRSPLEQSQVGTISGLYVCEMVTARA
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHCCCHHHHHHHHHHHHHH
LVERNAVTIHMTTFAPDESPRSLQLYTVDPATTYTAAKMVADEGLADHIDMNFGCPMPKV
HHHCCCEEEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHCCCHHHCCCCCCCCCCHH
TRRGGGAALPYKRRLFGQIVAAAVRATEGTKIPVTVKFRIGIDDEHHTHLDAGRIAEAEG
HCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCC
AAAVALHARTAAQRYSGTADWAQIAQLKQQVRTIPVLGNGDIYDASDALAMMAVTGCDGV
CEEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCHHEEEEEEECCCCE
VIGRGCLGRPWLFAELSAAFTGRPAPAPPTLGEVAEIVRRHGKLLVAHFGEDKGMRDIRK
EECCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
HIAWYLHGFPAGSELRNALALVKTLDELDCLLNRLDGAVPFPDAAIGPRGRQGSPAWVAL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEC
PDSWLTDPDDCTVPIGADIMESGG
CCCCCCCCCCCEECCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002