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The map label for this gene is pstA1

Identifier: 221230607

GI number: 221230607

Start: 2489056

End: 2489970

Strand: Reverse

Name: pstA1

Synonym: MLBr_02093

Alternate gene names: 221230607

Gene position: 2489970-2489056 (Counterclockwise)

Preceding gene: 221230608

Following gene: 221230605

Centisome position: 76.19

GC content: 57.49

Gene sequence:

>915_bases
ATGAATCTTGATACCCTCGACCGACCGGTCAAAGCGGTAGTGTTGCGCCCCATCAGCGTGCGACGCCGCATCAAAAACAA
CGTTGCGACAATGTTTTTCTTCACCTCGTTTCTCATTGCGCTGGTTCCGCTAGTTTGGCTGTTGTCAGTCGTGGTCGCGC
GAGGTTTCTACGCTGTAACTCGGTTCGACTGGTGGACCCACTCGCTACGCGGTGTGATGCCAGAAGAGTTTGCCGGCGGA
GTATACCACGCGCTGTATGGGTCGTTGGTGCAGGCTGTGGTGGCCGCCATCATGGCCGTGCCGCTGGGATCGATGACAGC
CGTATATCTGGTGGAATACGGTTCTGGCCCGTTCGTACGGGTGACCACGTTCATGGTTGACGTGCTCGCCGGAGTGCCCT
CGATCGTAGCGGCGTTGTTTATCTTCTGCCTGTGGATCGCCACCCTGGGATTCCAACAATCTGCCTTCGCTGTGTCGTTG
GCGTTGGTTTTGTTGATGTTGCCAGTGGTGGTGCGGTCTACCGAGGAAATACTGAGGTTGGTTCCTGACGAACTGCGAGA
AGCCTGCTACGCGTTGGGTATTCCGAAATGGAAGACAATTGTGCGCATTGTCTTTCCAATCGCGACGCCGGGCATCATCT
CCGGTGTCTTGTTGTCCATTGCGCGCGTCATCGGCGAAACTGCGCCGGTGCTCGTCCTGGTTGGGTACAGCCGTTCCATC
AACTTCGACATTTTTGACGGCAACATGGCCTCGTTGCCGCTGCTGATCTACACCGAACTGACCAATCCCGAATACGCGGG
TTTTCTGCGGGTTTGGGGCGCGGCACTGACTCTGATCATCTTGGTTGCCGGGATCAATCTCTTCGGCGCGGCGTTCCGCT
TCCTGGCTACCCGGCGGTGCAGCGGGTGCCGGTGA

Upstream 100 bases:

>100_bases
CAGCAGGATTTGCACTGTTCTTCCTGACGTTCGTCGTCAACGCGGTCGCTCGCGCAATCTCCGGCGGAAGGGTGAACGGA
TAAGCTGCGGTGAGCTCTCG

Downstream 100 bases:

>100_bases
CACCGGGCGCGTTAGGTCTCCTGGTCTCCTCGCTCGGCAACCGTGCTGATGCGATCCCGAAAGCCACCATATAAGCGAAG
TCCTTGCTGGTGATCAGGAT

Product: membrane-bound component of phosphate transport

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MNLDTLDRPVKAVVLRPISVRRRIKNNVATMFFFTSFLIALVPLVWLLSVVVARGFYAVTRFDWWTHSLRGVMPEEFAGG
VYHALYGSLVQAVVAAIMAVPLGSMTAVYLVEYGSGPFVRVTTFMVDVLAGVPSIVAALFIFCLWIATLGFQQSAFAVSL
ALVLLMLPVVVRSTEEILRLVPDELREACYALGIPKWKTIVRIVFPIATPGIISGVLLSIARVIGETAPVLVLVGYSRSI
NFDIFDGNMASLPLLIYTELTNPEYAGFLRVWGAALTLIILVAGINLFGAAFRFLATRRCSGCR

Sequences:

>Translated_304_residues
MNLDTLDRPVKAVVLRPISVRRRIKNNVATMFFFTSFLIALVPLVWLLSVVVARGFYAVTRFDWWTHSLRGVMPEEFAGG
VYHALYGSLVQAVVAAIMAVPLGSMTAVYLVEYGSGPFVRVTTFMVDVLAGVPSIVAALFIFCLWIATLGFQQSAFAVSL
ALVLLMLPVVVRSTEEILRLVPDELREACYALGIPKWKTIVRIVFPIATPGIISGVLLSIARVIGETAPVLVLVGYSRSI
NFDIFDGNMASLPLLIYTELTNPEYAGFLRVWGAALTLIILVAGINLFGAAFRFLATRRCSGCR
>Mature_304_residues
MNLDTLDRPVKAVVLRPISVRRRIKNNVATMFFFTSFLIALVPLVWLLSVVVARGFYAVTRFDWWTHSLRGVMPEEFAGG
VYHALYGSLVQAVVAAIMAVPLGSMTAVYLVEYGSGPFVRVTTFMVDVLAGVPSIVAALFIFCLWIATLGFQQSAFAVSL
ALVLLMLPVVVRSTEEILRLVPDELREACYALGIPKWKTIVRIVFPIATPGIISGVLLSIARVIGETAPVLVLVGYSRSI
NFDIFDGNMASLPLLIYTELTNPEYAGFLRVWGAALTLIILVAGINLFGAAFRFLATRRCSGCR

Specific function: Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane

COG id: COG0581

COG function: function code P; ABC-type phosphate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI1790163, Length=277, Percent_Identity=35.0180505415162, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1790164, Length=190, Percent_Identity=27.8947368421053, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PSTA_MYCLE (Q50097)

Other databases:

- EMBL:   U15184
- EMBL:   AL583924
- PIR:   H87170
- RefSeq:   NP_302393.1
- ProteinModelPortal:   Q50097
- SMR:   Q50097
- EnsemblBacteria:   EBMYCT00000029319
- GeneID:   908938
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML2093
- NMPDR:   fig|272631.1.peg.1265
- Leproma:   ML2093
- GeneTree:   EBGT00050000015074
- HOGENOM:   HBG705496
- OMA:   ITFFVDV
- ProtClustDB:   CLSK881166
- BioCyc:   MLEP272631:ML2093-MONOMER
- InterPro:   IPR000515
- InterPro:   IPR005672
- TIGRFAMs:   TIGR00974

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 33310; Mature: 33310

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x227d76f0)-; HASH(0x451a3850)-; HASH(0x451a2794)-; HASH(0x451a1f78)-; HASH(0x451a2b24)-; HASH(0x451a244c)-;

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDTLDRPVKAVVLRPISVRRRIKNNVATMFFFTSFLIALVPLVWLLSVVVARGFYAVT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
RFDWWTHSLRGVMPEEFAGGVYHALYGSLVQAVVAAIMAVPLGSMTAVYLVEYGSGPFVR
HHHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCCHH
VTTFMVDVLAGVPSIVAALFIFCLWIATLGFQQSAFAVSLALVLLMLPVVVRSTEEILRL
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHH
VPDELREACYALGIPKWKTIVRIVFPIATPGIISGVLLSIARVIGETAPVLVLVGYSRSI
CCHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
NFDIFDGNMASLPLLIYTELTNPEYAGFLRVWGAALTLIILVAGINLFGAAFRFLATRRC
CEEEECCCHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGCR
CCCH
>Mature Secondary Structure
MNLDTLDRPVKAVVLRPISVRRRIKNNVATMFFFTSFLIALVPLVWLLSVVVARGFYAVT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
RFDWWTHSLRGVMPEEFAGGVYHALYGSLVQAVVAAIMAVPLGSMTAVYLVEYGSGPFVR
HHHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCCHH
VTTFMVDVLAGVPSIVAALFIFCLWIATLGFQQSAFAVSLALVLLMLPVVVRSTEEILRL
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHH
VPDELREACYALGIPKWKTIVRIVFPIATPGIISGVLLSIARVIGETAPVLVLVGYSRSI
CCHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
NFDIFDGNMASLPLLIYTELTNPEYAGFLRVWGAALTLIILVAGINLFGAAFRFLATRRC
CEEEECCCHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGCR
CCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11234002