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The map label for this gene is glcB

Identifier: 221230593

GI number: 221230593

Start: 2457921

End: 2460116

Strand: Direct

Name: glcB

Synonym: MLBr_02069

Alternate gene names: 221230593

Gene position: 2457921-2460116 (Clockwise)

Preceding gene: 221230592

Following gene: 221230594

Centisome position: 75.21

GC content: 59.43

Gene sequence:

>2196_bases
ATGACTGATCGCGTGTCGGCGGGGAATTTGCGCGTCGCCCGTGTGCTTTACGACTTCGTCAACAACGAAGCCCTGCCTGG
CACCGACATCAACCCAAACAGCTTCTGGTCGGGCGTCGCCAAGGTCGTCGCCGACCTCACCCCGCAAAACCAAAGTTTGC
TGAACTCCCGCGATGAGCTGCAGGCCCAGATTGACAAATGGCACCGCCATCGTGTGATCGAGCCATTCGACGTGGATGCT
TATCGGCAGTTCCTCATCGATATCGGCTATCTGCTGCCTGAACCCGATGACTTCACCATCAGCACCTCAGGAGTAGACGA
CGAGATCACCATGACCGCCGGCCCGCAGCTGGTGGTGCCGGTACTTAACGCACGGTTCGCACTCAACGCCGCCAACGCCC
GCTGGGGCTCCCTTTACGACGCTCTATATGGCACCGACACCATACCGGAAACCGAGGGAGCCGAAAAGGGCTCTGAGTAC
AACAAGATTCGCGGCGACAAGGTGATCGCCTACGCACGCAAGTTTATGGATCAGGCCGTCCCGCTGGCTTCTGACTCATG
GACTAACGCTACGGGAGTGAGCATCTTCGACGGCCAGCTTCAGATCGCCATCGGCACGAATTCCACCGGATTAGCCAGTC
CCGAAAAGTTTGTCGGCTACAATCGCCAGTTGCGATCCTCCAACTGGTCGGTGCTGTTGGCCAATCACGGCCTGCACATC
GAGGTCTTGATCGACCCGGAATCACCAATAGGCAAGACTGACCCCGTCGGCATCAAGGATGTGATCCTGGAATCGGCCAT
CACCACGATCATGGACTTCGAAGACTCAGTCACTGCCGTCGACGCCGACGACAAGGTACGCGGCTACCGCAACTGGCTCG
GCCTGAACAAAGGTGACTTAACCGAGGAAGTCAACAAGGACGGCAAGACCTTCACCCGCGTGCTCAACGCCGACCGCAGC
TACACCACGCCCGACGGCGGTGAATTGACCCTGCCCGGGCGCAGCCTGTTGTTCGTCCGCAACGTGGGCCATCTGACGAC
CAGCGACGCGATTCTCGTGGACGGAGGTGACGGTCAGGAAAAAGAAGTGTTCGAGGGCATCATAGATGCAGTGTTCACCG
GTCTGGCCGCAATCCACGGACTCAAAACCGGCGAAGCTAATGGCCCACTGACCAACAGCCGCACCGGTTCCATCTACATC
GTCAAGCCGAAAATGCACGGTCCCGCGGAGGTTGCGTTCACTTGCGAGCTGTTCAGCCGCGTCGAAGACGTGCTGGGGTT
GCCGCAGGGCACCTTGAAGGTCGGCATCATGGATGAGGAGCGGCGTACGACACTCAATTTGAAGGCGTGCATCAAGGCCG
CCGCCGATCGGGTCGTGTTTATCAACACTGGCTTCTTGGACCGCACCGGCGACGAGATCCACACCTCAATGGAGGCCGGC
CCGATGATTCGTAAGGGTGCGATGAAAAACAGCACCTGGATCAAAGCCTACGAGGACGCCAATGTTGACATCGGACTAGC
CGCAGGCTTCAAGGGCAAAGCACAGATCGGCAAGGGCATGTGGGCAATGACCGAATTAATGGCTGATATGGTCGAGCAGA
AGATCGGCCAGCCTAAGGCGGGCGCCACCACCGCGTGGGTACCCTCGCCCACTGCGGCCACGCTGCATGCGATGCACTAC
CACCAGGTCGACGTGGCTGCCGTGCAACAAGAACTGACCGGCCAGAGACGTGCCACCGTTGACCAGCTGTTGACCATTCC
GTTGGCCAAGGAACTGGCCTGGGCTCCCGAGGAGATCCGCGAGGAGGTCGATAATGACTGTCAGTCCATCCTCGGTTATG
TGGTGCGCTGGGTGGACCAGGGTATCGGTTGCTCGAAGGTGCCTGACATCCACAATGTGGCACTAATGGAGGACCGCGCC
ACCCTGCGGATCTCGAGCCAGTTGCTAGCAAACTGGCTGCGCCACGGCGTAATCACCAGCGAGGACGTGCGGGCCAGCCT
GGAACGGATGGCGCCGTTAGTCGATCAGCAAAACGCCGAAGACCCAGCATATCGCCCGATGGCGCCTAATTTCGATGACA
GCATCGCGTTCCTAGCGGCGCAGGAACTGATACTGTCCGGCGCACAACAGCCCAACGGCTATACCGAGCCGATCCTGCAT
CGCCGTCGTCGCGAATTCAAGGCACAGAACCGGTGA

Upstream 100 bases:

>100_bases
CGCTCGTCAGGCTGTTGGCCGGTAGGCTTATGCGCGTAGACCGTGAGAGCCACATCGGGGACCGTTGGGGCCGCCAGTAA
GCCCAATATGGAGGAGAACC

Downstream 100 bases:

>100_bases
CGGATGCAGGCTGCTGGCGGCCAGCTGTGAGAGCACCACCAGCCGCGCCGCGGCTAAGGACAGAATCGGGTCCATCTAGA
TAGAACCGCTGCCTTACGCC

Product: malate synthase G

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 731; Mature: 730

Protein sequence:

>731_residues
MTDRVSAGNLRVARVLYDFVNNEALPGTDINPNSFWSGVAKVVADLTPQNQSLLNSRDELQAQIDKWHRHRVIEPFDVDA
YRQFLIDIGYLLPEPDDFTISTSGVDDEITMTAGPQLVVPVLNARFALNAANARWGSLYDALYGTDTIPETEGAEKGSEY
NKIRGDKVIAYARKFMDQAVPLASDSWTNATGVSIFDGQLQIAIGTNSTGLASPEKFVGYNRQLRSSNWSVLLANHGLHI
EVLIDPESPIGKTDPVGIKDVILESAITTIMDFEDSVTAVDADDKVRGYRNWLGLNKGDLTEEVNKDGKTFTRVLNADRS
YTTPDGGELTLPGRSLLFVRNVGHLTTSDAILVDGGDGQEKEVFEGIIDAVFTGLAAIHGLKTGEANGPLTNSRTGSIYI
VKPKMHGPAEVAFTCELFSRVEDVLGLPQGTLKVGIMDEERRTTLNLKACIKAAADRVVFINTGFLDRTGDEIHTSMEAG
PMIRKGAMKNSTWIKAYEDANVDIGLAAGFKGKAQIGKGMWAMTELMADMVEQKIGQPKAGATTAWVPSPTAATLHAMHY
HQVDVAAVQQELTGQRRATVDQLLTIPLAKELAWAPEEIREEVDNDCQSILGYVVRWVDQGIGCSKVPDIHNVALMEDRA
TLRISSQLLANWLRHGVITSEDVRASLERMAPLVDQQNAEDPAYRPMAPNFDDSIAFLAAQELILSGAQQPNGYTEPILH
RRRREFKAQNR

Sequences:

>Translated_731_residues
MTDRVSAGNLRVARVLYDFVNNEALPGTDINPNSFWSGVAKVVADLTPQNQSLLNSRDELQAQIDKWHRHRVIEPFDVDA
YRQFLIDIGYLLPEPDDFTISTSGVDDEITMTAGPQLVVPVLNARFALNAANARWGSLYDALYGTDTIPETEGAEKGSEY
NKIRGDKVIAYARKFMDQAVPLASDSWTNATGVSIFDGQLQIAIGTNSTGLASPEKFVGYNRQLRSSNWSVLLANHGLHI
EVLIDPESPIGKTDPVGIKDVILESAITTIMDFEDSVTAVDADDKVRGYRNWLGLNKGDLTEEVNKDGKTFTRVLNADRS
YTTPDGGELTLPGRSLLFVRNVGHLTTSDAILVDGGDGQEKEVFEGIIDAVFTGLAAIHGLKTGEANGPLTNSRTGSIYI
VKPKMHGPAEVAFTCELFSRVEDVLGLPQGTLKVGIMDEERRTTLNLKACIKAAADRVVFINTGFLDRTGDEIHTSMEAG
PMIRKGAMKNSTWIKAYEDANVDIGLAAGFKGKAQIGKGMWAMTELMADMVEQKIGQPKAGATTAWVPSPTAATLHAMHY
HQVDVAAVQQELTGQRRATVDQLLTIPLAKELAWAPEEIREEVDNDCQSILGYVVRWVDQGIGCSKVPDIHNVALMEDRA
TLRISSQLLANWLRHGVITSEDVRASLERMAPLVDQQNAEDPAYRPMAPNFDDSIAFLAAQELILSGAQQPNGYTEPILH
RRRREFKAQNR
>Mature_730_residues
TDRVSAGNLRVARVLYDFVNNEALPGTDINPNSFWSGVAKVVADLTPQNQSLLNSRDELQAQIDKWHRHRVIEPFDVDAY
RQFLIDIGYLLPEPDDFTISTSGVDDEITMTAGPQLVVPVLNARFALNAANARWGSLYDALYGTDTIPETEGAEKGSEYN
KIRGDKVIAYARKFMDQAVPLASDSWTNATGVSIFDGQLQIAIGTNSTGLASPEKFVGYNRQLRSSNWSVLLANHGLHIE
VLIDPESPIGKTDPVGIKDVILESAITTIMDFEDSVTAVDADDKVRGYRNWLGLNKGDLTEEVNKDGKTFTRVLNADRSY
TTPDGGELTLPGRSLLFVRNVGHLTTSDAILVDGGDGQEKEVFEGIIDAVFTGLAAIHGLKTGEANGPLTNSRTGSIYIV
KPKMHGPAEVAFTCELFSRVEDVLGLPQGTLKVGIMDEERRTTLNLKACIKAAADRVVFINTGFLDRTGDEIHTSMEAGP
MIRKGAMKNSTWIKAYEDANVDIGLAAGFKGKAQIGKGMWAMTELMADMVEQKIGQPKAGATTAWVPSPTAATLHAMHYH
QVDVAAVQQELTGQRRATVDQLLTIPLAKELAWAPEEIREEVDNDCQSILGYVVRWVDQGIGCSKVPDIHNVALMEDRAT
LRISSQLLANWLRHGVITSEDVRASLERMAPLVDQQNAEDPAYRPMAPNFDDSIAFLAAQELILSGAQQPNGYTEPILHR
RRREFKAQNR

Specific function: Accounts For Almost The Entire Malate-Synthesizing Activity In Cells Metabolizing Glyoxylate. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family. GlcB subfamily

Homologues:

Organism=Escherichia coli, GI1789348, Length=729, Percent_Identity=56.9272976680384, Blast_Score=830, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MASZ_MYCLB (B8ZSN3)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002504009.1
- EnsemblBacteria:   EBMYCT00000085840
- GeneID:   7325139
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00070000032288
- HOGENOM:   HBG350005
- OMA:   PKMHGPD
- ProtClustDB:   PRK02999
- GO:   GO:0005737
- HAMAP:   MF_00641
- InterPro:   IPR011076
- InterPro:   IPR001465
- InterPro:   IPR006253
- PANTHER:   PTHR21631:SF1
- TIGRFAMs:   TIGR01345

Pfam domain/function: PF01274 Malate_synthase; SSF51645 Malat_synth_like

EC number: =2.3.3.9

Molecular weight: Translated: 80143; Mature: 80012

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: ACT_SITE 340-340 ACT_SITE 638-638

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRVSAGNLRVARVLYDFVNNEALPGTDINPNSFWSGVAKVVADLTPQNQSLLNSRDEL
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCHHHH
QAQIDKWHRHRVIEPFDVDAYRQFLIDIGYLLPEPDDFTISTSGVDDEITMTAGPQLVVP
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEECCCCEEEEE
VLNARFALNAANARWGSLYDALYGTDTIPETEGAEKGSEYNKIRGDKVIAYARKFMDQAV
ECCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHC
PLASDSWTNATGVSIFDGQLQIAIGTNSTGLASPEKFVGYNRQLRSSNWSVLLANHGLHI
CCCCCCCCCCCCCEEECCEEEEEEECCCCCCCCCHHHHCCCHHCCCCCCEEEEECCCEEE
EVLIDPESPIGKTDPVGIKDVILESAITTIMDFEDSVTAVDADDKVRGYRNWLGLNKGDL
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCCCCH
TEEVNKDGKTFTRVLNADRSYTTPDGGELTLPGRSLLFVRNVGHLTTSDAILVDGGDGQE
HHHHCCCHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEECCCCEECCCEEEEECCCCCH
KEVFEGIIDAVFTGLAAIHGLKTGEANGPLTNSRTGSIYIVKPKMHGPAEVAFTCELFSR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
VEDVLGLPQGTLKVGIMDEERRTTLNLKACIKAAADRVVFINTGFLDRTGDEIHTSMEAG
HHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCC
PMIRKGAMKNSTWIKAYEDANVDIGLAAGFKGKAQIGKGMWAMTELMADMVEQKIGQPKA
CHHHHCCCCCCCEEEEECCCCCEEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCC
GATTAWVPSPTAATLHAMHYHQVDVAAVQQELTGQRRATVDQLLTIPLAKELAWAPEEIR
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHH
EEVDNDCQSILGYVVRWVDQGIGCSKVPDIHNVALMEDRATLRISSQLLANWLRHGVITS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCC
EDVRASLERMAPLVDQQNAEDPAYRPMAPNFDDSIAFLAAQELILSGAQQPNGYTEPILH
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHH
RRRREFKAQNR
HHHHHHHCCCC
>Mature Secondary Structure 
TDRVSAGNLRVARVLYDFVNNEALPGTDINPNSFWSGVAKVVADLTPQNQSLLNSRDEL
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCHHHH
QAQIDKWHRHRVIEPFDVDAYRQFLIDIGYLLPEPDDFTISTSGVDDEITMTAGPQLVVP
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEECCCCEEEEE
VLNARFALNAANARWGSLYDALYGTDTIPETEGAEKGSEYNKIRGDKVIAYARKFMDQAV
ECCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHC
PLASDSWTNATGVSIFDGQLQIAIGTNSTGLASPEKFVGYNRQLRSSNWSVLLANHGLHI
CCCCCCCCCCCCCEEECCEEEEEEECCCCCCCCCHHHHCCCHHCCCCCCEEEEECCCEEE
EVLIDPESPIGKTDPVGIKDVILESAITTIMDFEDSVTAVDADDKVRGYRNWLGLNKGDL
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCCCCH
TEEVNKDGKTFTRVLNADRSYTTPDGGELTLPGRSLLFVRNVGHLTTSDAILVDGGDGQE
HHHHCCCHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEECCCCEECCCEEEEECCCCCH
KEVFEGIIDAVFTGLAAIHGLKTGEANGPLTNSRTGSIYIVKPKMHGPAEVAFTCELFSR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
VEDVLGLPQGTLKVGIMDEERRTTLNLKACIKAAADRVVFINTGFLDRTGDEIHTSMEAG
HHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCC
PMIRKGAMKNSTWIKAYEDANVDIGLAAGFKGKAQIGKGMWAMTELMADMVEQKIGQPKA
CHHHHCCCCCCCEEEEECCCCCEEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCC
GATTAWVPSPTAATLHAMHYHQVDVAAVQQELTGQRRATVDQLLTIPLAKELAWAPEEIR
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHH
EEVDNDCQSILGYVVRWVDQGIGCSKVPDIHNVALMEDRATLRISSQLLANWLRHGVITS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCC
EDVRASLERMAPLVDQQNAEDPAYRPMAPNFDDSIAFLAAQELILSGAQQPNGYTEPILH
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHH
RRRREFKAQNR
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA